LOCUS       NM_001108471            1963 bp    mRNA    linear   ROD 17-JUL-2023
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2M (Ube2m), mRNA.
ACCESSION   NM_001108471 XM_001062179 XM_341790
VERSION     NM_001108471.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg
            LA, Neale G, Murray PJ, Roussel MF and Schulman BA.
  TITLE     E2-RING expansion of the NEDD8 cascade confers specificity to
            cullin modification
  JOURNAL   Mol Cell 33 (4), 483-495 (2009)
   PUBMED   19250909
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM and Schulman
            BA.
  TITLE     Structural basis for recruitment of Ubc12 by an E2 binding domain
            in NEDD8's E1
  JOURNAL   Mol Cell 17 (3), 341-350 (2005)
   PUBMED   15694336
REFERENCE   3  (bases 1 to 1963)
  AUTHORS   Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF and
            Schulman BA.
  TITLE     A unique E1-E2 interaction required for optimal conjugation of the
            ubiquitin-like protein NEDD8
  JOURNAL   Nat Struct Mol Biol 11 (10), 927-935 (2004)
   PUBMED   15361859
REFERENCE   4  (bases 1 to 1963)
  AUTHORS   Chen Y, Liu W, McPhie DL, Hassinger L and Neve RL.
  TITLE     APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is
            increased in Alzheimer's disease brain
  JOURNAL   J Cell Biol 163 (1), 27-33 (2003)
   PUBMED   14557245
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474090.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_341790.3, XM_001062179.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CV118842.1, CO556027.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132291, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1963
                     /gene="Ube2m"
                     /note="ubiquitin-conjugating enzyme E2M"
                     /db_xref="GeneID:361509"
                     /db_xref="RGD:1307614"
     exon            1..682
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
     CDS             574..1125
                     /gene="Ube2m"
                     /EC_number="6.3.2.19"
                     /note="ubiquitin-conjugating enzyme E2M (UBC12 homolog,
                     yeast)"
                     /codon_start=1
                     /product="NEDD8-conjugating enzyme Ubc12"
                     /protein_id="NP_001101941.1"
                     /db_xref="GeneID:361509"
                     /db_xref="RGD:1307614"
                     /translation="MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLP
                     KTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHP
                     NIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLF
                     EQNVQRSMRGGYIGSTYFERCLK"
     exon            683..777
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
     exon            778..816
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
     exon            817..920
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
     exon            921..984
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1963
                     /gene="Ube2m"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgcagccga gccaggaaaa accgaagcgg acagacgacg gaaagtgccg aagcggcggg
       61 gtgcccgagg ccgttgccga cctccgcatc gaagccgcgc ctgccgcgga agctgagcct
      121 ccaccggtac cctgcggtac cctgccgccg caagcgcggc tgccgcctcc tccttccacc
      181 tcaccgcagc ctcctagcaa agggagcaag taggcgggag agcggcggac cggtaaaggc
      241 cggcgtccgc agccgcagcg gggactcaca agacccgagc ggaagagggg gaggagggcg
      301 cgggggccgt agcaggggcg gggccgagga tagccgagtc ctcccgagca cttccgaaca
      361 cacccgaggt gctaagccag gccagagccg gactacggga gccgaggcgg gccgtgcggt
      421 gggcgcggag cagcgcggca ggccgggcgg gcggcagcgg aggaggccgc ggtagcgggt
      481 ccgaggagcg gaagcggcgc aggcggcggc ggggggccgg gtggccgggg tcccgggccc
      541 cgcggcggct gcggcggcgg cggcggcggc aggatgatca agctgttctc gctgaagcag
      601 cagaagaagg aggaggagtc ggccggcggc accaagggca gcagcaagaa ggcgtcggcg
      661 gcgcagctgc ggattcagaa ggacattaac gagctgaacc tgcccaagac gtgtgacatc
      721 agcttctcag acccagacga cctcctcaac ttcaaactgg ttatctgtcc tgatgaaggc
      781 ttctacaaga gcggcaagtt tgtgttcagc tttaaggtgg gacagggtta cccacatgac
      841 cctcccaagg tgaagtgtga aacaatggtt tatcatccca acattgacct cgagggcaac
      901 gtctgcctca acatcctcag agaggactgg aagccagtcc ttacgataaa ctccataatt
      961 tatggcctgc agtatctctt cttggagccc aaccccgagg acccactgaa caaggaggct
     1021 gccgaggtcc tgcagaacaa tcggcggctg tttgaacaaa acgtgcagcg ctccatgaga
     1081 ggtggttaca tcgggtccac ctacttcgag cgctgcctga aatagggtta gcaattaccc
     1141 atccctgcca gggttactgg cccaggcatc ccctgcaaat atttattggg ggcccaggta
     1201 gggtgtgtgg ggtagccttg gcccctgcct tggccttgcc tctcctccct gtcacctgcc
     1261 cctagttatt ttttttgacc accacgtgat tatggtcggt gctgcctccc ccgacctgct
     1321 cagcgatggg aaatgaattg gcttgtttag cgccccccct cccgctgggt gctgtccaac
     1381 cccccactct tgactgtggg gtaagtgggc aatgggcctg ggtcaccagg ctcaagcaac
     1441 ccaccccacc accactggag gtcccaccag gctattaaag gggaatgtta ctgcatggcc
     1501 tctgcctctt ccttctgtgg ggtgggaggt acaagtgagg tccaccagcg tctataatga
     1561 agataaattt gtggccttgg gttgggtggg accactcact gggatgaggc gcaagcaatg
     1621 gaagaatcag gtaatttcag ctcctggccc ttacaccaac ataagggagc ctacaagtca
     1681 tttccagtct tgccccttta tgccatgtgt gggcccgcca atttggttcc tgccaggcac
     1741 ttgctaagat acgataaatg atctgcccaa aactgggtgg agtatgggcc ttccagaatg
     1801 aatagccagt cctgctgcgc tcccgccgtg ggcctgcgat gctggcttgg gtggggcttg
     1861 taccttctgc tgaggtgcgg ggtcctttgc gcatgcgctt ttatggctgg ctgtgcgcgt
     1921 gcgtcttagc acttcccagc attcccggag agggtgggtc gtg
//
