LOCUS       NM_001007655            1636 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2),
            mRNA.
ACCESSION   NM_001007655 XM_008764352 XM_216595
VERSION     NM_001007655.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   van de Weijer,M.L., Bassik,M.C., Luteijn,R.D., Voorburg,C.M.,
            Lohuis,M.A., Kremmer,E., Hoeben,R.C., LeProust,E.M., Chen,S.,
            Hoelen,H., Ressing,M.E., Patena,W., Weissman,J.S., McManus,M.T.,
            Wiertz,E.J. and Lebbink,R.J.
  TITLE     A high-coverage shRNA screen identifies TMEM129 as an E3 ligase
            involved in ER-associated protein degradation
  JOURNAL   Nat Commun 5, 3832 (2014)
   PUBMED   24807418
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   3  (bases 1 to 1636)
  AUTHORS   El Khouri,E., Le Pavec,G., Toledano,M.B. and Delaunay-Moisan,A.
  TITLE     RNF185 is a novel E3 ligase of endoplasmic reticulum-associated
            degradation (ERAD) that targets cystic fibrosis transmembrane
            conductance regulator (CFTR)
  JOURNAL   J Biol Chem 288 (43), 31177-31191 (2013)
   PUBMED   24019521
REFERENCE   4  (bases 1 to 1636)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   5  (bases 1 to 1636)
  AUTHORS   Tiwari,S. and Weissman,A.M.
  TITLE     Endoplasmic reticulum (ER)-associated degradation of T cell
            receptor subunits. Involvement of ER-associated
            ubiquitin-conjugating enzymes (E2s)
  JOURNAL   J Biol Chem 276 (19), 16193-16200 (2001)
   PUBMED   11278356
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1 and BC079115.1.
            
            On or before Jun 23, 2015 this sequence version replaced
            XM_008764352.1, NM_001007655.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079115.1, SRR21887622.1710135.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                JAXUCZ010000005.1  171815612-171815621
            11-1636             BC079115.1         1-1626
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..1636
                     /gene="Ube2j2"
                     /note="ubiquitin-conjugating enzyme E2, J2"
                     /db_xref="GeneID:298689"
                     /db_xref="RGD:1359523"
     exon            1..335
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..498
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     CDS             463..1278
                     /gene="Ube2j2"
                     /EC_number="6.3.2.19"
                     /note="ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog,
                     yeast)"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 J2"
                     /protein_id="NP_001007656.2"
                     /db_xref="GeneID:298689"
                     /db_xref="RGD:1359523"
                     /translation="MHQAKALDSARKMSSNSNKRAPTTATQRLKQDYLRIKKDPVPYI
                     CAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCN
                     TRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLV
                     FNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLN
                     GHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE"
     exon            499..629
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            630..670
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            671..773
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            774..912
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            913..993
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1614
                     /gene="Ube2j2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgtccctt gagcggccat cttggaagct gagcgaccgt agcagcggag aggctgcggc
       61 gggtgcggtg ggcttagtcc cagcgcgggt gcggctcacg gcgcgggccc cactcttccg
      121 caccctgctc cggccttggc cacagcgaac tgaacgcggt agcggcctca gggagaggtg
      181 agcgctcgcg ggctgcgcgg ggccttgggc cgctcgttcg ccccgccgga gccggctgca
      241 gcggcgggcg gggactcggg gactccacac gtcgggtccc gttcgttacg tcgccggctg
      301 tgtccggacc cggagcgtcc cttcgcggac ccgaggtgct gggcaagttg agctctctgg
      361 cccacatggc ttctgaagct aagaacttgg ggacaatgct ttctggaagc atttcttggc
      421 catgctggac tgtaaccttg ttcctggtgt gagcattctt ggatgcatca ggcaaaagcc
      481 cttgattctg ccagaaagat gagcagtaac agcaataaga gagctccaac aactgcgaca
      541 caaaggttga aacaggacta ccttcgaatt aagaaagacc cagtgcctta catctgtgcg
      601 gagcccctcc cttcaaatat tcttgaatgg cattatgttg tccgaggccc tgagatgact
      661 ccttatgaag gtggctatta tcatggaaaa ctaatttttc ccagagaatt tccatttaag
      721 cctcctagta tttacatgat aacccccaat ggaagattta agtgcaacac aaggctgtgt
      781 ctctccatca cagatttcca tccagataca tggaatccag cgtggtccgt ctccaccatt
      841 ctaacagggc tcctgagctt tatggtggag aaaggcccca ccctggggag catagagaca
      901 tcggacttca cgaaaaaaca gctggcagca cagagtctag tgtttaattt aaaagataaa
      961 gtcttctgtg aattatttcc tgaagttgtg gaggaaatta aacagaaaca gaaagcacaa
     1021 gatgaactaa gtaacaggcc ccagaatctt cctttgccag acgtggttcc tgatggggag
     1081 atgcaccgtg gccagaatgg gatccagctc ctcaatgggc atgcacctgc tgctggccca
     1141 aaccttgctg ggctcccaca ggccaacagg catcatggac tcctgggcgg cgctttggcg
     1201 aacttgtttg ttatagttgg gtttgcggcc tttgcctaca cggtcaaata cgtgctgaga
     1261 agcatagcac aggagtgagg ttcacaagcc acaacctgca gttgctgctg agggacacag
     1321 gggccagagc ttgatgcagg cgagacggac atgctgctga gcacagggtg gcctgccgga
     1381 ctcctgggct tcacttttta aaaaccttaa acagaaagca aaaaccaaaa tgtgatctgg
     1441 aggagattgg aggttgacat ggacagtgcc actcacgctt tggttttact gcttatctcc
     1501 tgtgttgtct tttgaacctg tttagtaaac ctgttttgtt tgttttgttt tgttttgttt
     1561 ttcattttat tagatttggt cacttaaaaa tataaaacgt taatgcctat caaaaaaaaa
     1621 aaaaaaaaaa aaaaaa
//
