LOCUS       NM_001106642            3515 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2, J1 (Ube2j1),
            mRNA.
ACCESSION   NM_001106642 XM_001063852 XM_039109427 XM_216362
VERSION     NM_001106642.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Zhong,Y., Shen,H., Wang,Y., Yang,Y., Yang,P. and Fang,S.
  TITLE     Identification of ERAD components essential for dislocation of the
            null Hong Kong variant of alpha-1-antitrypsin (NHK)
  JOURNAL   Biochem Biophys Res Commun 458 (2), 424-428 (2015)
   PUBMED   25660456
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Koenig,P.A., Nicholls,P.K., Schmidt,F.I., Hagiwara,M., Maruyama,T.,
            Frydman,G.H., Watson,N., Page,D.C. and Ploegh,H.L.
  TITLE     The E2 ubiquitin-conjugating enzyme UBE2J1 is required for
            spermiogenesis in mice
  JOURNAL   J Biol Chem 289 (50), 34490-34502 (2014)
   PUBMED   25320092
REFERENCE   3  (bases 1 to 3515)
  AUTHORS   Menon,M.B., Tiedje,C., Lafera,J., Ronkina,N., Konen,T.,
            Kotlyarov,A. and Gaestel,M.
  TITLE     Endoplasmic reticulum-associated ubiquitin-conjugating enzyme
            Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in
            MK2-mediated TNFalpha production
  JOURNAL   Biochem J 456 (2), 163-172 (2013)
   PUBMED   24020373
  REMARK    Erratum:[Biochem J. 2014 Jan 1;457(1):229]
REFERENCE   4  (bases 1 to 3515)
  AUTHORS   El Khouri,E., Le Pavec,G., Toledano,M.B. and Delaunay-Moisan,A.
  TITLE     RNF185 is a novel E3 ligase of endoplasmic reticulum-associated
            degradation (ERAD) that targets cystic fibrosis transmembrane
            conductance regulator (CFTR)
  JOURNAL   J Biol Chem 288 (43), 31177-31191 (2013)
   PUBMED   24019521
REFERENCE   5  (bases 1 to 3515)
  AUTHORS   Sato,T., Sako,Y., Sho,M., Momohara,M., Suico,M.A., Shuto,T.,
            Nishitoh,H., Okiyoneda,T., Kokame,K., Kaneko,M., Taura,M.,
            Miyata,M., Chosa,K., Koga,T., Morino-Koga,S., Wada,I. and Kai,H.
  TITLE     STT3B-dependent posttranslational N-glycosylation as a surveillance
            system for secretory protein
  JOURNAL   Mol Cell 47 (1), 99-110 (2012)
   PUBMED   22607976
REFERENCE   6  (bases 1 to 3515)
  AUTHORS   Burr,M.L., Cano,F., Svobodova,S., Boyle,L.H., Boname,J.M. and
            Lehner,P.J.
  TITLE     HRD1 and UBE2J1 target misfolded MHC class I heavy chains for
            endoplasmic reticulum-associated degradation
  JOURNAL   Proc Natl Acad Sci U S A 108 (5), 2034-2039 (2011)
   PUBMED   21245296
REFERENCE   7  (bases 1 to 3515)
  AUTHORS   Imai,Y., Soda,M., Inoue,H., Hattori,N., Mizuno,Y. and Takahashi,R.
  TITLE     An unfolded putative transmembrane polypeptide, which can lead to
            endoplasmic reticulum stress, is a substrate of Parkin
  JOURNAL   Cell 105 (7), 891-902 (2001)
   PUBMED   11439185
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On or before Mar 4, 2021 this sequence version replaced
            XM_039109427.1, NM_001106642.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.125995.1,
                                           SRR26360172.1401466.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               JAXUCZ010000005.1  52218807-52219035
            230-303             JAXUCZ010000005.1  52222892-52222965
            304-435             JAXUCZ010000005.1  52223985-52224116
            436-520             JAXUCZ010000005.1  52226699-52226783
            521-626             JAXUCZ010000005.1  52227511-52227616
            627-756             JAXUCZ010000005.1  52229442-52229571
            757-876             JAXUCZ010000005.1  52230776-52230895
            877-3515            JAXUCZ010000005.1  52235167-52237805
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q21"
     gene            1..3515
                     /gene="Ube2j1"
                     /note="ubiquitin-conjugating enzyme E2, J1"
                     /db_xref="GeneID:297961"
                     /db_xref="RGD:1305067"
     exon            1..229
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     CDS             199..1155
                     /gene="Ube2j1"
                     /EC_number="6.3.2.19"
                     /note="ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog,
                     yeast)"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 J1"
                     /protein_id="NP_001100112.2"
                     /db_xref="GeneID:297961"
                     /db_xref="RGD:1305067"
                     /translation="METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFT
                     VRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETW
                     QPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVL
                     LPLKSGIDSSQADQEAKELARQISFKAEVNSSGKTIAESDLNQSFSLNDSQDDLPTTF
                     QGAAASTSYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRSSASPDVLQGQP
                     PRANHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL"
     exon            230..303
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            304..435
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            436..520
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            521..626
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            627..756
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            757..876
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
     exon            877..3515
                     /gene="Ube2j1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgtccgca gcccggcgcc ccgttcggcg acgccccggc gcccggccgg ccctcaggga
       61 ggcggacccc gcggcccgca cctcgcccgc ggtcgggctg gactgggcgg tgtcagcgga
      121 ggccctcgtc aggcgcgtcc gccgcgcgac acccgggcag ctgcggcgcc gacgagagcg
      181 gcagcgggga ggtccgccat ggagacccgc tataacctga agagcccggc tgttaaacga
      241 ttgatgaaag aagccgcaga attgaaagat ccaacagatc attaccacgc tcagcctctg
      301 gaggataacc tttttgaatg gcacttcaca gttagagggc ccccagattc cgattttgat
      361 ggaggagttt atcatggacg catagtactg ccaccagagt accccatgaa accacccagc
      421 attattctgc tgacggctaa tggacgattt gaagtgggca agaagatctg tttaagcatc
      481 tcaggacatc atcctgagac ctggcagcct tcatggagca taaggacagc cctgctggcc
      541 atcattgggt ttatgccaac taaaggagag ggagccatag gttctctaga ctacacgccc
      601 gaggagagga gagcacttgc caagaaatca caagacttct gttgtgaggg ctgtggctct
      661 gccatgaagg atgtcctgtt gcctttaaaa tctggaatcg attcaagcca ggctgaccaa
      721 gaagccaaag aactggctag acaaattagt tttaaggccg aagtcaattc atctggaaag
      781 actatcgctg aatcagactt aaaccagtct ttctcactaa atgattcaca agacgattta
      841 cctacaacat tccagggtgc tgcagcaagc acatcgtatg gcgcccagaa tccatcagga
      901 gcacccttgc cacaacctac ccagccagct cctaagaata cctccatgag ccctcggcaa
      961 cgtagagccc agcaacagag tcagagaagg tcgtctgctt cccccgatgt actccaaggc
     1021 cagccaccaa gagccaacca cacggagcac ggcgggtcag ccatgctgat catcatcctg
     1081 actttggcac tggcagctct catcttccga cggatatatc tggccaacga gtacatattt
     1141 gactttgagt tataatgctg gtctatgact tcagagctgt gactcagttg cgtcattaaa
     1201 cattctgcat tgggtataac ctaaggattg tttacaaaag attattttgt atttaccctt
     1261 cgttactttt tttattgtta taaattcatt gtgctataag tagacattca ggctgtggcc
     1321 cagcagtgtg tcggaagcca tagtccttgt cttactattt aatctacttc acagacaagt
     1381 aacttactgc ggttgctcac acccacgctg cttttctaca gagcgcagtg cttttctgtg
     1441 tgtcctttgg cattctcagc taagaaggtg tttccattga gtgttatggc tcctcagtct
     1501 cgcctgtgag tccttctcta gttagacagt gagggagaag atggcggtgg ggcacactgc
     1561 tcactgtctt cttgagcagt gtcattcata ggagagacat ggacggggat cgcccactct
     1621 gtcatctttg tactggaaac tgtgtgcagt gagaatgatt ttatttcaga catttatgaa
     1681 gatctttgtt atattttgtt ttggatttta cttgaagagg agagactctg cagcaccttg
     1741 gtatgcgcca tccccaatat ggacacaaat gaaagaaaca ggcttccaca gccttctcag
     1801 gccagggtcc aagccaagca gttgtttgat ttttccttca caagtaggtg aggaggtcac
     1861 caggtttgaa tctttacttt gatctgacat ggtttgaatt ttatgccctg ctctgtaatc
     1921 actctagctt ttcagtattc cagagatatg cttagtattc aaattttaat aggaaactct
     1981 gggcccaagg tacagcgatg gattaaccac aaataaacct ttatttagaa ctgttcactg
     2041 gcctaagaaa catgtaatga agaatccatc tacccttagt gccagcaagt gccccagacg
     2101 ctgacctgta ctcttgtcat cacagtgcct agtgagactg ctcaggacaa ggttgccttc
     2161 ccatgctcct tcagcaacct cgaggcactt cggagggccc tgcattgctg actcgactgt
     2221 ccccagcttt taaatatatc atgctcttga agcccatttg gaagtttgtt tttgtttttt
     2281 gttttttttt ttaaggagaa ctgaatataa atggtatttg ggttcagtcc acaactgtta
     2341 gctcatgcat cttttaggta tgtgtgggag ggtagttgta aagtcctcat gtggttttaa
     2401 ctaaatataa ttatcggcaa gataaaacag ctcgcaaaat cactttaaag gtgagcctgg
     2461 ttctcactcc tgtcatgtga tgttgggatc tgttgccact gaagagaatt cccctttgta
     2521 tttggtagct gggttttgtt gttactgttg ttatcttaac accagaagtt ttaaaaggga
     2581 ccgaatgcta tatataatat aatcttccag tttaatagag tagattttta tagttattta
     2641 aggattgttt atagatgagg ttggcaaaaa tctaaaaagc ccaaggactt tgtgaagact
     2701 tcatgtaagg aagactatag attaccattt taaaaataat gttcttgcca tgaaaaagca
     2761 tattcttagt ttataatatg agaaactgtg gttccatgat ctagtaagaa gtcagtaacg
     2821 ccgtgggaag cctgggtggc cttcccactt aggagaagaa ccctggcgct gtcgccctgg
     2881 gctgagacag ctcatatgct agagccgtgc ccctccagtc atccccctcc agttaggcct
     2941 ggctagtgtt tggagggagc tctgcggtct tgctgtgctt ctgtggtcac ttgtgctggg
     3001 tgtggaggga gatctcatcc actccctcac tgtcgctaac tggctacctc cttatgcatg
     3061 ttggcaattt ttagcacctg atacatacat caaagtggta cgttttgttt ttacccatga
     3121 tccgctatct aaaaattttc ctccaacccc caccttgggg ttagtggact catggaaggc
     3181 ctctgttgtc aaagcctcag tttaaccccc cagatccggc ataaggcaca aaccatgtag
     3241 aagctggtag gtaggtctta aattcttgtc tccccagttc tcagattcaa aactcaaggc
     3301 tttttcactg accagcatat catatgacta cacatcaaat tagtcaaaat agcatgcttg
     3361 aaaaaatatg cctgttttgc ttgtagcagt ctagcccgcg ggagccttcc agatctgtat
     3421 gtgggtgggg ctctgatgta gcactttacg ttttcttgct gctgctgttg gtttattcta
     3481 ctgaaataaa tgatttcatc aagcttctca gtgaa
//
