LOCUS       NM_001172127            4266 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2H (Ube2h), mRNA.
ACCESSION   NM_001172127
VERSION     NM_001172127.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Yi,J.S., Park,J.S., Ham,Y.M., Nguyen,N., Lee,N.R., Hong,J.,
            Kim,B.W., Lee,H., Lee,C.S., Jeong,B.C., Song,H.K., Cho,H.,
            Kim,Y.K., Lee,J.S., Park,K.S., Shin,H., Choi,I., Lee,S.H.,
            Park,W.J., Park,S.Y., Choi,C.S., Lin,P., Karunasiri,M., Tan,T.,
            Duann,P., Zhu,H., Ma,J. and Ko,Y.G.
  TITLE     MG53-induced IRS-1 ubiquitination negatively regulates skeletal
            myogenesis and insulin signalling
  JOURNAL   Nat Commun 4, 2354 (2013)
   PUBMED   23965929
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1 and CB739657.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582424.2227758.1,
                                           SRR26360176.1412269.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676785 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                JAXUCZ010000004.1  59897488-59897583   c
            97-173              JAXUCZ010000004.1  59851304-59851380   c
            174-184             JAXUCZ010000004.1  59850023-59850033   c
            185-641             CB739657.1         1-457
            642-4266            JAXUCZ010000004.1  59802102-59805726   c
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..4266
                     /gene="Ube2h"
                     /note="ubiquitin-conjugating enzyme E2H"
                     /db_xref="GeneID:296956"
                     /db_xref="RGD:2319288"
     exon            1..96
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..595
                     /gene="Ube2h"
                     /EC_number="6.3.2.19"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 H"
                     /protein_id="NP_001165598.1"
                     /db_xref="GeneID:296956"
                     /db_xref="RGD:2319288"
                     /translation="MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGT
                     PYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLT
                     NIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEG
                     TGDSSSESSMSDFSEDEAQDMEL"
     exon            97..173
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     exon            174..248
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     exon            249..288
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     exon            289..341
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     exon            342..470
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
     exon            471..4266
                     /gene="Ube2h"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcggcgc aggcgacggg gaggcagcgg cagcggcgac accatgtcat cccccagtcc
       61 cggcaagagg cggatggaca cggacgtagt caagctcatt gaaagcaaac atgaggttac
      121 aatcctagga ggactcaatg aatttgttgt caagttttat ggaccacaag gaacaccata
      181 tgaaggcggg gtgtggaaag ttagagtgga ccttcctgat aaataccctt tcaaatctcc
      241 atctatagga ttcatgaata aaattttcca tcccaacatt gatgaagcgt caggaactgt
      301 gtgtctagat gtaattaatc aaacttggac agctctctat gatcttacca atatatttga
      361 gtccttcctg ccccaattgc tggcctatcc taaccccata gatcctctca atggtgatgc
      421 tgcagccatg tacctccacc gaccagaaga gtacaagcag aaaattaaag agtacatcca
      481 gaagtatgca acggaggagg ccctgaagga gcaggaggag ggcactggcg acagctcatc
      541 ggagagttct atgtccgact tctcagaaga tgaggcccag gacatggagt tgtagtagaa
      601 aaggcatctg cttttcagaa agactattat atcctaacca tgagaagcag actctaatat
      661 tcatatttaa acaaagcaat tttttttatt actaaacaag gtttttatga ataatagcat
      721 tgatatatat atattatata tcacccttta gatcttgatt tcttggtcat ttctcaacct
      781 gaggtgcgta gcatattccc acattccatt ttggtagcag tatgcggtct gaatgcatgc
      841 atgcgtaagt ccatttggcc aagtcagcct gtgcgctact gaactactgt caagagattg
      901 ccgctctgag gtaaacatga gttaatgaca agtttgcttc ttttattgat ggttccaaag
      961 aaacaaacca aattctacca gctcttggat gaagatagaa ccgtgctttt ccagagtgga
     1021 aaggaaaggg ttgagacgcg tgctttgaga gcctgtgaag ctgcgctgtc tgtgtcagcc
     1081 actccatagt cctaagtggc cctttgtgag gagcagcgtg agacagagtt gatgagaagc
     1141 gagacttagg gctttggggt ttttttggct cagatttaag tgtgtttttc taggtagatt
     1201 cccgccctcc ctgctgccag acctgcagcc acattggttt tttgccttgg agaacatttg
     1261 ggaagcattt tctgaaccca tttccttttg ggggtgtcca gtttgtcatc cagcagagca
     1321 ttttgagaga acaggactga aggagaaagg tgactggaag acgccgagtg acatctgatc
     1381 cagcgccggc cggccgggcc tgtcggagac cattgccctg gactgagctg tgacactgga
     1441 agcagttctg tttggtgttt tgtttttctt ttttttttta gctttgtgtg aattgtactt
     1501 agacaagtta caggtttaat tttcactacc aagagagtct cctccctgga caaggtgctc
     1561 agtggaccat caaatggagg aagactggcc ttgttgcttg agccctgcac aacccttgga
     1621 actccagctt agttttcctc ccagaagctg ccaactgctg tgggagtgtg ggtagtgtgg
     1681 gtatccgaga cccaaaacat cctaaatttg aaatgggtaa cagttgaaac aaactgaagt
     1741 ttttgtacca aagtggctcg aagtgggaag aaccacttta gtaaattgtc atggaggcag
     1801 gttgagggga caccactttc tccctctgca gtaacagaca gacagacaga cagacagctg
     1861 gaagtgcctg gctggctgct accggccaac tgccatccag tgtgcccggc agtttgagac
     1921 ctgactctgg agggaatttg gagaccgttt agagagcagc tctgctctgt atttttgccg
     1981 aaaccagagc agtagacgta gccaatgtaa gaccttctga agacattttg aatgtcccac
     2041 tggactaacc tttctcttga tgtgtgtttt tagtttagaa gtgctatatt ccacaagggt
     2101 taagtcaatg aggttggaga acacctatct tttttctttt ttaattcaaa aagcacaatc
     2161 agtctttcct ttgaaaatct ataaaagtac aacttttagc atgattattt tcctaaataa
     2221 aaagacaaat aatttactta ttttacgtta attacgggca tgaagcaaaa ggtttctttg
     2281 gggaaaatgc agttctctag tgctggggtt ggttcactgc actgtccctg gcaccctggc
     2341 ttgttgaaag gtggctttag ctgtgtgcca tgtgaccgtt tgcctgccac gctctgttgt
     2401 ggttagggga gtttctgctc acacttgcag cttgctgccc tgcagattag ccctttgctc
     2461 atgctacagc cttcctgtgg gtgctacctt gggcaggctt gaactgctgg gtgatggtcg
     2521 tgagttcaaa caattaaaaa tgtgggtttt ttttcaatac tatataaaag tagtgctctg
     2581 attacctcct cctcaaaaaa aaaatccaga taattgcttt tgatattgtt ggggaaaaaa
     2641 taaaacagag ccaactttgg gaaacaggtg tgagcagcta cagctcttga cccttaccgg
     2701 gggccacttc gctccaatga agagacatca ttccttcccg tgcagggctg acagtgcaga
     2761 gtgtccatcc agacacatga tgaaggtgtc actggttctg cactgcagtt cccatgcctg
     2821 cccccaagtc actccaccct ggactttgtg caccagttct ggttctgtgc atggtgcagc
     2881 tctctggacc ttgactctgt tagaaagtgt gtaagctccc tggcccttcc ctgctcctag
     2941 ttattagatg accataccca taattgcaaa ggaccagaag gaaagtctga ctgtgactga
     3001 gaggactctg ggtccccgca gtaagctggt gggttctgca gcagggctgc tgtaacacca
     3061 agcccctttg gaggggcagc agcaagccag gctgctcgct ccttcccacc ctttatctac
     3121 cattttttga ggtcctgtct tgtgctttac tagtgaaagc catcctttgc ccttgttgat
     3181 gacttgagct cttgccaaac accgacaagc agagtggctg cagcttggca gcccaaacct
     3241 gccacgttcc tgtgcccagg gaagcccaga accggggcca gtgggctatg ttccgtggtt
     3301 gccaacctag accctggcat ttgaaattct ttttgtcagc agtctccacc ctctcacgag
     3361 tgctgagagc gtcaaccagc atttctattg atgagagtta ccagaagacg cagcatctct
     3421 ctctctccag caccactgtt ttttggtcca tttcaaacgc tgttctaatt gccaaccggc
     3481 tgatctaggc tcctgaggag ttaccccttc tcagaggaac tgcgccctgg aaaggactcc
     3541 ccgaagttct agttggcctt gacctgatac ttgcattttc taggccccct tttggggtgg
     3601 ctaagaatca tgacagatcc tctgccagtt tctagtcttg ctgggtctgg tttctgagta
     3661 ctggcattgg attttaaatg gcatgtgacc cttctatgtt ttagtgactc agaaaagtga
     3721 agccatttgc aaaagttagt ttgttttgtc aacttctaac tgggtcatag ccttgctgag
     3781 tgaaaccaac tcatttctga ttgggcacaa agaactgcat tctttggact tctgaatcca
     3841 tgttcatctt cctctggcca ctgaacacct tggccagctc tttaggaatt ggatggtgtg
     3901 atggcccttg gggagccatg gtgtgcagca ttttgctcac ctgaggagtg gccagggctc
     3961 ttggtttagc aattactcgg ctgtgggtgt gggaagaaat ggcctttaat tactgattgt
     4021 atttggtaca catgcgtgat acttgaagag aaaagggact taccagccct taattgaaat
     4081 ctaagctttt tactgctttc cccccctggc cctatctcct cccttccgcc ttccttgcat
     4141 tgtgatgatc tagtgggcat gtctgtcacc tggacaaatg cctcctctga ctataacctc
     4201 cttaatagtt gtgtataatg aaaactgtaa acttttttaa ataaaatgtg tatatacctt
     4261 ggcaaa
//
