LOCUS       NM_001399660            1471 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2),
            transcript variant 3, mRNA.
ACCESSION   NM_001399660 XM_039093447
VERSION     NM_001399660.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Mizukami,S., Yafune,A., Watanabe,Y., Nakajima,K., Jin,M.,
            Yoshida,T. and Shibutani,M.
  TITLE     Identification of epigenetically downregulated Tmem70 and Ube2e2 in
            rat liver after 28-day treatment with hepatocarcinogenic
            thioacetamide showing gene product downregulation in hepatocellular
            preneoplastic and neoplastic lesions produced by tumor promotion
  JOURNAL   Toxicol Lett 266, 13-22 (2017)
   PUBMED   27914986
  REMARK    GeneRIF: The effect of thioacetamide on neoplastic liver changes
            and hypermethylation and downregulation of proteins, especially
            Tmem70 and Ube2e2, are reported.
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Eze,I.C., Imboden,M., Kumar,A., von Eckardstein,A., Stolz,D.,
            Gerbase,M.W., Kunzli,N., Pons,M., Kronenberg,F., Schindler,C. and
            Probst-Hensch,N.
  TITLE     Air pollution and diabetes association: Modification by type 2
            diabetes genetic risk score
  JOURNAL   Environ Int 94, 263-271 (2016)
   PUBMED   27281273
REFERENCE   3  (bases 1 to 1471)
  AUTHORS   Shibata,E., Abbas,T., Huang,X., Wohlschlegel,J.A. and Dutta,A.
  TITLE     Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G
            ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase
            complex
  JOURNAL   Mol Cell Biol 31 (15), 3136-3145 (2011)
   PUBMED   21628527
REFERENCE   4  (bases 1 to 1471)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   5  (bases 1 to 1471)
  AUTHORS   Takeuchi,T., Iwahara,S., Saeki,Y., Sasajima,H. and Yokosawa,H.
  TITLE     Link between the ubiquitin conjugation system and the ISG15
            conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2
            enzyme
  JOURNAL   J Biochem 138 (6), 711-719 (2005)
   PUBMED   16428300
REFERENCE   6  (bases 1 to 1471)
  AUTHORS   Kimura,M., Hattori,T., Matsuda,Y., Yoshioka,T., Sumi,N., Umeda,Y.,
            Nakashima,S. and Okano,Y.
  TITLE     cDNA cloning, characterization, and chromosome mapping of UBE2E2
            encoding a human ubiquitin-conjugating E2 enzyme
  JOURNAL   Cytogenet Cell Genet 78 (2), 107-111 (1997)
   PUBMED   9371400
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Jan 12, 2022 this sequence version replaced XM_039093447.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360172.130027.1, CK480708.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               JAXUCZ010000015.1  9411067-9411197
            132-315             JAXUCZ010000015.1  9417093-9417276
            316-366             JAXUCZ010000015.1  9429735-9429785
            367-499             JAXUCZ010000015.1  9631270-9631402
            500-647             JAXUCZ010000015.1  9666120-9666267
            648-1471            JAXUCZ010000015.1  9687908-9688731
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p16"
     gene            1..1471
                     /gene="Ube2e2"
                     /note="ubiquitin-conjugating enzyme E2E 2"
                     /db_xref="GeneID:361013"
                     /db_xref="RGD:1305644"
     exon            1..131
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            132..315
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..745
                     /gene="Ube2e2"
                     /EC_number="6.3.2.19"
                     /note="isoform 2 is encoded by transcript variant 3;
                     ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog,
                     yeast)"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 E2 isoform 2"
                     /protein_id="NP_001386589.1"
                     /db_xref="GeneID:361013"
                     /db_xref="RGD:1305644"
                     /translation="MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGK
                     ISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGG
                     VFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSI
                     CSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT"
     exon            316..366
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            367..499
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            500..647
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            648..1471
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcattcctg gacagaacta ggctcagcag gacctttcct gcagcagtta tggggtaaag
       61 cgaggcttca tccgggccca cgcgaagttt cccaaccgcg gggcaggtgg gtcctgcacg
      121 agcacaagct ggatctaaga tgtccaccga ggcacagaga gttgatgaca gtccaagcac
      181 tagcggagga agttctgatg gcgatcaacg ggagagtgtt cagcaggaac ctgagcggga
      241 gcaagttcag cccaagaaga aggaggggaa gatatccagc aagacggctg cgaagctgtc
      301 gaccagtgcc aaaagaattc agaaggagct tgcagaaatc acgctggacc cccctcccaa
      361 ctgtagtgct ggacccaaag gagacaacat ttatgaatgg aggtcaacta tattgggacc
      421 cccaggatct gtctatgaag gaggggtgtt ctttcttgac attacctttt caccagacta
      481 tccgtttaaa ccccctaagg tcaccttccg aacaagaatc tatcactgta atatcaacag
      541 ccagggcgtg atctgcctgg acatcctcaa ggacaactgg agtccggccc tgaccatctc
      601 taaagtcctc ctgtccatct gctcactact tacagactgc aaccctgccg accctctggt
      661 aggcagtatt gccacacagt acatgaccaa cagagccgag cacgaccgga tggccagaca
      721 gtggaccaag cggtacgcca cctaggggcc tacgtgtgcc agccgtgtgc aagcacattc
      781 accaagtgca tcaatagccc acctgcccct ccagacctcc gttctgtttc ctgtttttat
      841 taaattttaa actgttttgt gatggtatgt cgtttgtccc tcctccttcc tgccgccacc
      901 tcctcccctt ctttccggct ctctttctcc cttttactcc cttttcggtt tctgttaact
      961 tgaaaggttc ggggtccccc actgccgtgt ggatgggagc gtctgttcta agtacaaacc
     1021 ctgttggagc tgtcctggtg aggcttttca cacagagctt cgtgttaccg tgtggtttgg
     1081 gttttgttgt tgttgatgtt cgttctatct tttttctttt tcttttctgt gctctttggg
     1141 gggaacacac gttaagaatt atatctggtt tctacttaaa tgtagtgctt agggttaatt
     1201 ttttgtactg aagtctttat tggtgggtgc atgctactgg gaacaaggtt ttgtacaaaa
     1261 ggtccaatca gaatcactgt gcattactga gaccctgtct atcacaagcc ttctgtcccc
     1321 acacagaaga ctgtcagagt gaacaaaata atatgtattt tgatttactt aagtgcttgt
     1381 aaatttctta gggacctgcc acttttgact gtggatcagt tgatgtatac ttgtattatt
     1441 aatgcctcaa taaatcactg tggcggatcg a
//
