LOCUS       NM_001393772            2453 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2D 2 (Ube2d2),
            mRNA.
ACCESSION   NM_001393772
VERSION     NM_001393772.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Polge,C., Koulmann,N., Claustre,A., Jarzaguet,M., Serrurier,B.,
            Combaret,L., Bechet,D., Bigard,X., Attaix,D. and Taillandier,D.
  TITLE     UBE2D2 is not involved in MuRF1-dependent muscle wasting during
            hindlimb suspension
  JOURNAL   Int J Biochem Cell Biol 79, 488-493 (2016)
   PUBMED   27378730
  REMARK    GeneRIF: UBE2D2 is not the cognate ubiquitinating enzyme for MuRF1
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Starita,L.M., Pruneda,J.N., Lo,R.S., Fowler,D.M., Kim,H.J.,
            Hiatt,J.B., Shendure,J., Brzovic,P.S., Fields,S. and Klevit,R.E.
  TITLE     Activity-enhancing mutations in an E3 ubiquitin ligase identified
            by high-throughput mutagenesis
  JOURNAL   Proc Natl Acad Sci U S A 110 (14), E1263-E1272 (2013)
   PUBMED   23509263
REFERENCE   3  (bases 1 to 2453)
  AUTHORS   Hoxhaj,G., Najafov,A., Toth,R., Campbell,D.G., Prescott,A.R. and
            MacKintosh,C.
  TITLE     ZNRF2 is released from membranes by growth factors and, together
            with ZNRF1, regulates the Na+/K+ATPase
  JOURNAL   J Cell Sci 125 (Pt 19), 4662-4675 (2012)
   PUBMED   22797923
  REMARK    Erratum:[J Cell Sci. 2022 Mar 15;135(6):jcs259936. doi:
            10.1242/jcs.259936.. PMID: 35267011]
REFERENCE   4  (bases 1 to 2453)
  AUTHORS   Rong,J., Chen,L., Toth,J.I., Tcherpakov,M., Petroski,M.D. and
            Reed,J.C.
  TITLE     Bifunctional apoptosis regulator (BAR), an endoplasmic reticulum
            (ER)-associated E3 ubiquitin ligase, modulates BI-1 protein
            stability and function in ER Stress
  JOURNAL   J Biol Chem 286 (2), 1453-1463 (2011)
   PUBMED   21068390
REFERENCE   5  (bases 1 to 2453)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   6  (bases 1 to 2453)
  AUTHORS   Rajendra,R., Malegaonkar,D., Pungaliya,P., Marshall,H., Rasheed,Z.,
            Brownell,J., Liu,L.F., Lutzker,S., Saleem,A. and Rubin,E.H.
  TITLE     Topors functions as an E3 ubiquitin ligase with specific E2 enzymes
            and ubiquitinates p53
  JOURNAL   J Biol Chem 279 (35), 36440-36444 (2004)
   PUBMED   15247280
REFERENCE   7  (bases 1 to 2453)
  AUTHORS   Kikkert,M., Doolman,R., Dai,M., Avner,R., Hassink,G., van
            Voorden,S., Thanedar,S., Roitelman,J., Chau,V. and Wiertz,E.
  TITLE     Human HRD1 is an E3 ubiquitin ligase involved in degradation of
            proteins from the endoplasmic reticulum
  JOURNAL   J Biol Chem 279 (5), 3525-3534 (2004)
   PUBMED   14593114
REFERENCE   8  (bases 1 to 2453)
  AUTHORS   Anan,T., Nagata,Y., Koga,H., Honda,Y., Yabuki,N., Miyamoto,C.,
            Kuwano,A., Matsuda,I., Endo,F., Saya,H. and Nakao,M.
  TITLE     Human ubiquitin-protein ligase Nedd4: expression, subcellular
            localization and selective interaction with ubiquitin-conjugating
            enzymes
  JOURNAL   Genes Cells 3 (11), 751-763 (1998)
   PUBMED   9990509
REFERENCE   9  (bases 1 to 2453)
  AUTHORS   Wing,S.S., Bedard,N., Morales,C., Hingamp,P. and Trasler,J.
  TITLE     A novel rat homolog of the Saccharomyces cerevisiae
            ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct
            biochemical features is induced during spermatogenesis
  JOURNAL   Mol Cell Biol 16 (8), 4064-4072 (1996)
   PUBMED   8754804
REFERENCE   10 (bases 1 to 2453)
  AUTHORS   Wing,S.S. and Jain,P.
  TITLE     Molecular cloning, expression and characterization of a ubiquitin
            conjugation enzyme (E2(17)kB) highly expressed in rat testis
  JOURNAL   Biochem J 305 (Pt 1) (Pt 1), 125-132 (1995)
   PUBMED   7826319
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.1792118.1,
                                           SRR26360194.446173.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               JAXUCZ010000018.1  27638399-27638826
            429-492             JAXUCZ010000018.1  27660887-27660950
            493-524             JAXUCZ010000018.1  27671374-27671405
            525-602             JAXUCZ010000018.1  27671486-27671563
            603-708             JAXUCZ010000018.1  27671644-27671749
            709-802             JAXUCZ010000018.1  27674028-27674121
            803-2453            JAXUCZ010000018.1  27678736-27680386
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p11"
     gene            1..2453
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /note="ubiquitin-conjugating enzyme E2D 2"
                     /db_xref="GeneID:641452"
                     /db_xref="RGD:1591897"
     exon            1..428
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     CDS             405..848
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="E2(17)KB 2; ubiquitin-protein ligase D2; ubiquitin
                     carrier protein D2; ubiquitin-conjugating enzyme E2-17 kDa
                     2; ubiquitin-conjugating enzyme E2(17)KB 2; ubc2e
                     ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme
                     E2D 2 (UBC4/5 homolog, yeast); E2 ubiquitin-conjugating
                     enzyme D2; (E3-independent) E2 ubiquitin-conjugating
                     enzyme D2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 D2"
                     /protein_id="NP_001380701.1"
                     /db_xref="GeneID:641452"
                     /db_xref="RGD:1591897"
                     /translation="MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSP
                     YQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKV
                     LLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM"
     exon            429..492
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     exon            493..524
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     exon            525..602
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     exon            603..708
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     exon            709..802
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
     exon            803..2453
                     /gene="Ube2d2"
                     /gene_synonym="E217kB; Ubc2e"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgcgtccct tagtccacct gcaccaggca ggaaagcagg cttccctggc ttcgccaccg
       61 taagtcgctc ggcgatcctg gagcttgggg ctcttcctcc caggcggagg gatccacggc
      121 agccgaggag gcggcgctga gcctacgagg aagaggcagg ctacggcggc ggcggctgtg
      181 gcagcagcgc ataaaggggc cgccgccggg tgatgcggtg accgctgcgg caggcccaga
      241 agctgagtgg gcctcggccc tcagctcgtc gtatccgcgc tccggagctc cccgttctcc
      301 ggtcgactcg cgaccgctcg ggcggtctca agccccttag ccccttcccc gtccctcccc
      361 cgtccccgtc cccgccccgg ggccagccgc caccgtttcc caccatggct ctgaagagaa
      421 tccacaagga attgaatgac ctggctcgag atcctccagc acagtgttca gcaggtcctg
      481 ttggagatga tatgtttcat tggcaggcta caataatggg gccaaatgac agcccctatc
      541 agggtggagt atttttcttg actattcatt tcccaacaga ttaccccttc aaaccgccta
      601 aggttgcatt tacaacaaga atttatcatc caaatattaa cagtaatggc agcatttgtc
      661 ttgatattct acggtcacag tggtctccag cactaactat ttcaaaagta cttttgtcca
      721 tctgttctct gttgtgtgat cccaatccag atgatccttt agtgcctgag attgctcgga
      781 tctacaaaac agatagagaa aagtacaaca gaatagctcg ggaatggact cagaagtatg
      841 cgatgtaatt aaagaaatta ttggataacc tctacaaata aagctagggg aactctgaaa
      901 gagagaaagt ccttttgatt tccatttgac tgctttctat gagcccacgc ctcatcttcc
      961 cctgtgcaca tgtttacctg acacagcagt gctgcgtgtt gtacatactt ggaacaacaa
     1021 actagaaata ctgtacttct gtaccaacat tgcctcctag cagagaagtg tgtgtgtgag
     1081 aagccagttc tccgggcgta acctaggtgt gagactaaaa gctttcctta ttgacttaac
     1141 tttggataat ggcaagatgt gatgggtagt gggtacacag cgtgtgcttg aatgggagga
     1201 aaaagctcca gtgacccata ggagatggtc cttaagtgga ctccatctaa tcccctaaca
     1261 gtcgtgaaca gcatggtgaa gagcgtgaag ctgtttctgt attcctttca tcctgaaaga
     1321 cctacagctt aggagaaagt tatgaccaag cagagtaaac tacatgctgt attttaaggt
     1381 ctaagtttaa ctgatcaaca tttaaatgga ctggagctgt tagtacataa agtgtgatgg
     1441 actttgttcc caacactttc atatctccct gccctttcag ccttcctcct tttagcccct
     1501 ttccataatc tttggtttgt atgtggtttc tcagttaata catagctaat agctcttatt
     1561 tttcttatgt ttttaactgc tttaggccta tttcgatgta agggtgaaca ttcatttgat
     1621 ggaaacactt gtgtatattt aaagacccag ttgctcctct ggagcttgta cgttcaagaa
     1681 tgattaatct gtgtaataaa ctgattacta cagtcattac atataatttt gtgtgaatag
     1741 gctttttgat ttaaaaactt ggtctagcta agattagtgg tggatttttc ctcatccctg
     1801 aaatgttttc atttatattt gttgcatttc aaaatcatga aacaattcca gtttacatac
     1861 taaaagagat gtgagagtaa ttttattaaa caagatagag gcgtaagctt aaaggcattt
     1921 tcatgaaact tacatgtgca ccattccagg aacatgactg ttaaactaaa gaaatttgct
     1981 ttgttagtga aagtgagcag cacttcacta gaaccttacg acagtctctt ggtacatagg
     2041 acataggaca caaggtgttg ctgttgatga gctaattgat tgtaactata acgagtaact
     2101 gaccaaacct gggaaacatg agcacaatct tgtatcggag agtctacgtt attactttgc
     2161 actaacattt gcaggatgtc cattggtttt aaattgtact gtatgtggct ttttgaagtc
     2221 ttcctttgac tctagtaaaa tgtagtttgg aatttgtaaa caactgttaa tccagaaaca
     2281 ttcatgtgaa ctaggcaagt tatctcccct catccctttt cctaacctaa atgtaagcca
     2341 ggccagcttg aaggccatgg ctgacgctct ctagtcatca ggttctttga aatgcatctt
     2401 tacactcttg cacaaaaatt gaggaataaa tgaccactgc ttttggtttt aaa
//
