LOCUS       NM_031138               2109 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus ubiquitin-conjugating enzyme E2B (Ube2b), mRNA.
ACCESSION   NM_031138
VERSION     NM_031138.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Peng,Y., Zhang,Q., Cheng,H., Yan,G. and Xing,C.
  TITLE     Upregulation of ubiquitin conjugating enzyme E2B (Ube2b)
            ameliorates neuropathic pain by regulating Kcna2 (potassium
            voltage-gated channel subfamily A member 2) in primary afferent
            neurons
  JOURNAL   Bioengineered 12 (1), 7470-7480 (2021)
   PUBMED   34632937
  REMARK    GeneRIF: Upregulation of ubiquitin conjugating enzyme E2B (Ube2b)
            ameliorates neuropathic pain by regulating Kcna2 (potassium
            voltage-gated channel subfamily A member 2) in primary afferent
            neurons.
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Chen,Z.G., Wang,Y.J., Chen,R.S., Geng,F., Gan,C.L., Wang,W.S.,
            Liu,X., Zhou,H., He,L., Hu,G. and Liu,J.G.
  TITLE     Ube2b-dependent degradation of DNMT3a relieves a transcriptional
            brake on opiate-induced synaptic and behavioral plasticity
  JOURNAL   Mol Psychiatry 26 (4), 1162-1177 (2021)
   PUBMED   31576007
  REMARK    GeneRIF: Ube2b-dependent degradation of DNMT3a relieves a
            transcriptional brake on opiate-induced synaptic and behavioral
            plasticity.
REFERENCE   3  (bases 1 to 2109)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   4  (bases 1 to 2109)
  AUTHORS   Kim,J., Guermah,M., McGinty,R.K., Lee,J.S., Tang,Z., Milne,T.A.,
            Shilatifard,A., Muir,T.W. and Roeder,R.G.
  TITLE     RAD6-Mediated transcription-coupled H2B ubiquitylation directly
            stimulates H3K4 methylation in human cells
  JOURNAL   Cell 137 (3), 459-471 (2009)
   PUBMED   19410543
REFERENCE   5  (bases 1 to 2109)
  AUTHORS   Ryu,K.S., Choi,Y.S., Ko,J., Kim,S.O., Kim,H.J., Cheong,H.K.,
            Jeon,Y.H., Choi,B.S. and Cheong,C.
  TITLE     Direct characterization of E2-dependent target specificity and
            processivity using an artificial p27-linker-E2 ubiquitination
            system
  JOURNAL   BMB Rep 41 (12), 852-857 (2008)
   PUBMED   19123975
REFERENCE   6  (bases 1 to 2109)
  AUTHORS   Grootegoed,J.A., Baarends,W.M., Roest,H.P. and Hoeijmakers,J.H.
  TITLE     Knockout mouse model and gametogenic failure
  JOURNAL   Mol Cell Endocrinol 145 (1-2), 161-166 (1998)
   PUBMED   9922113
  REMARK    Review article
REFERENCE   7  (bases 1 to 2109)
  AUTHORS   Gantla,S., Bakker,C.T., Deocharan,B., Thummala,N.R., Zweiner,J.,
            Sinaasappel,M., Roy Chowdhury,J., Bosma,P.J. and Roy Chowdhury,N.
  TITLE     Splice-site mutations: a novel genetic mechanism of Crigler-Najjar
            syndrome type 1
  JOURNAL   Am J Hum Genet 62 (3), 585-592 (1998)
   PUBMED   9497253
REFERENCE   8  (bases 1 to 2109)
  AUTHORS   Wing,S.S. and Banville,D.
  TITLE     14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and
            regulation upon fasting and by insulin
  JOURNAL   Am J Physiol 267 (1 Pt 1), E39-E48 (1994)
   PUBMED   8048511
REFERENCE   9  (bases 1 to 2109)
  AUTHORS   Wing,S.S., Dumas,F. and Banville,D.
  TITLE     A rabbit reticulocyte ubiquitin carrier protein that supports
            ubiquitin-dependent proteolysis (E214k) is homologous to the yeast
            DNA repair gene RAD6
  JOURNAL   J Biol Chem 267 (10), 6495-6501 (1992)
   PUBMED   1313008
REFERENCE   10 (bases 1 to 2109)
  AUTHORS   Koken,M.H., Reynolds,P., Jaspers-Dekker,I., Prakash,L., Prakash,S.,
            Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Structural and functional conservation of two human homologs of the
            yeast DNA repair gene RAD6
  JOURNAL   Proc Natl Acad Sci U S A 88 (20), 8865-8869 (1991)
   PUBMED   1717990
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DN931313.1, BC070946.1 and BE111816.1.
            
            On Apr 19, 2007 this sequence version replaced NM_031138.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DN931313.1, BC070946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DN931313.1         31-37
            8-1690              BC070946.1         1-1683
            1691-2109           BE111816.1         1-419               c
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..2109
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /note="ubiquitin-conjugating enzyme E2B"
                     /db_xref="GeneID:81816"
                     /db_xref="RGD:708345"
     exon            1..145
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..560
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /EC_number="2.3.2.23"
                     /note="E2(14k); ubiquitin-protein ligase B; ubiquitin
                     carrier protein B; RAD6 homolog B; ubiquitin-conjugating
                     enzyme E2(14k); ubiquitin-conjugating enzyme E2B, RAD6
                     homology; ubiquitin conjugating enzyme;
                     ubiquitin-conjugating enzyme E2B (RAD6 homolog, S.
                     cerevisiae); E2 ubiquitin-conjugating enzyme B"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 B"
                     /protein_id="NP_112400.1"
                     /db_xref="GeneID:81816"
                     /db_xref="RGD:708345"
                     /translation="MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPE
                     GTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDV
                     SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS"
     exon            146..226
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
     exon            227..252
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
     exon            253..342
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
     exon            343..431
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
     exon            432..2096
                     /gene="Ube2b"
                     /gene_synonym="HR6B"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggcggcgg cgacccacag tgattcggcc gctgcgccgg ggggtggggg ggccgaacgg
       61 gactcttttc tttcagactg accgcggggc agctgcggag catgtcgacc ccggcccgta
      121 ggaggctcat gcgggatttc aagcgattgc aagaggaccc acctgtgggg gtcagtggtg
      181 caccatctga aaacaacatc atgcagtgga acgcagttat atttggacca gaagggacac
      241 cctttgaaga tggtactttt aaactagtaa tagaattttc tgaagaatat ccaaataaac
      301 caccaaccgt taggttttta tccaaaatgt ttcatccaaa tgtgtatgct gacggcagca
      361 tatgcttaga catcctgcag aaccgatgga gccccacgta cgacgtctcc tccatcttaa
      421 cttcaattca gtctctgttg gatgagccga atccaaacag tccggccaat agccaagcag
      481 cacagcttta tcaggaaaac aaacgggagt atgagaagag ggtttcggcc attgttgagc
      541 agagctggaa tgactcataa tagacacctg gtctgtccac ctttccatcg tcgttgtgta
      601 taatttacct cccagtagga aggctagcaa gttttaagtg ccacgggttt taaggattct
      661 gcagagaaaa aagaaaaagt ccttcagttt agaacctaca aaagcttgtg tatcttgatt
      721 aatgtacttt ttattgcatg gtgtgaacta agttattgct gcataaattt gtaatatatc
      781 ctgtttgtat ttttttccaa gtgtataatg ttggtgtgga gttttcatga cagaatatac
      841 acattttgta aatctgtact tttttcaaat attgaatgcc ttatttttga attctttaga
      901 tttttaaatt ggagaaaagc acttaaagtt ttttatatat gaatattaca tgtaaagctg
      961 ttaaaataca taacttcagt gcaagagact ttgtcactta tttccttacc tgtgcaggag
     1021 ggactgccaa gtctctgctc ttcgccaggt gagagcctca tttccaactg cagaaggtca
     1081 ctcttctgtt ttatcaagaa agtctgcaaa tttgattcta aaacctattc tgatctccgg
     1141 ctttattttg aatgtactta tgttagttca gttgagtaac tatagactta ctggtctggt
     1201 tctgtccagc tctgtatttg ggccacttgt taatgtgcgt ttctcatgta aactgtacct
     1261 tttgtgactt cacaattggc acctcgtcct cgagcagagc tgacttactc tgctgcgtgg
     1321 cagcacctcc acaggctttt gattaagaag aaagtaaaac catcaccatt caccaaatgc
     1381 cgtgccgcgg tgttagcagt ggccaggtgc gctggtttga gcaaggcaga ggtttggaag
     1441 gaatctcggt gtaacttgaa tatttgcaaa ctacctttta atgcggctgc gcgctacctg
     1501 tttattccgg aactgttgag caccagggcc tgccgagttg cttctcacct cctcagttgt
     1561 attcaagttg tactgcctct tagtttactg ggtagactta aaacacagta ttgtagaaag
     1621 ctaagacgaa gctatcctat gccttcaagt agtatagaaa atggaaaata tacaagtaaa
     1681 ttctgttgaa cctcttgtgt agtctctgta gtagttgaaa cagctattgc agtgagcccg
     1741 agtttcttgt catccatacc accctcctag agacatgctc tggtggcctc tccgctggaa
     1801 cattgggttg cagagtgacc atgtcttctg tttacaagca aagtttaaac ctgggatgga
     1861 taagaactga tactttcctt taggagttgt tatcccaagg ggttcatgag ttactggtat
     1921 gtgctgtttc tgcaagaccc caatctccgt tcccagttcc cacttcagac agttcacagt
     1981 tgctcataac tccagctccc cggggatcca aagcttctca ctaaatcaga cactggcatt
     2041 cacataaaca gacactacat aattaaaata aaagtattaa tttattaagc ttagaaaaaa
     2101 aaaaaaaaa
//
