LOCUS       NM_001044291             935 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1),
            mRNA.
ACCESSION   NM_001044291 XM_001079206
VERSION     NM_001044291.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Ivatt RM, Sanchez-Martinez A, Godena VK, Brown S, Ziviani E and
            Whitworth AJ.
  TITLE     Genome-wide RNAi screen identifies the Parkinson disease GWAS risk
            locus SREBF1 as a regulator of mitophagy
  JOURNAL   Proc Natl Acad Sci U S A 111 (23), 8494-8499 (2014)
   PUBMED   24912190
REFERENCE   2  (bases 1 to 935)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 935)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 935)
  AUTHORS   Wahl MC, Will CL and Luhrmann R.
  TITLE     The spliceosome: design principles of a dynamic RNP machine
  JOURNAL   Cell 136 (4), 701-718 (2009)
   PUBMED   19239890
  REMARK    Review article
REFERENCE   5  (bases 1 to 935)
  AUTHORS   Rzymski T, Grzmil P, Meinhardt A, Wolf S and Burfeind P.
  TITLE     PHF5A represents a bridge protein between splicing proteins and
            ATP-dependent helicases and is differentially expressed during
            mouse spermatogenesis
  JOURNAL   Cytogenet Genome Res 121 (3-4), 232-244 (2008)
   PUBMED   18758164
REFERENCE   6  (bases 1 to 935)
  AUTHORS   Cazalla D, Newton K and Caceres JF.
  TITLE     A novel SR-related protein is required for the second step of
            Pre-mRNA splicing
  JOURNAL   Mol Cell Biol 25 (8), 2969-2980 (2005)
   PUBMED   15798186
REFERENCE   7  (bases 1 to 935)
  AUTHORS   Jurica MS, Licklider LJ, Gygi SR, Grigorieff N and Moore MJ.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   8  (bases 1 to 935)
  AUTHORS   Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P,
            Sleeman J, Lamond A and Mann M.
  TITLE     Mass spectrometry and EST-database searching allows
            characterization of the multi-protein spliceosome complex
  JOURNAL   Nat Genet 20 (1), 46-50 (1998)
   PUBMED   9731529
REFERENCE   9  (bases 1 to 935)
  AUTHORS   Zamore PD and Green MR.
  TITLE     Biochemical characterization of U2 snRNP auxiliary factor: an
            essential pre-mRNA splicing factor with a novel intranuclear
            distribution
  JOURNAL   EMBO J 10 (1), 207-214 (1991)
   PUBMED   1824937
REFERENCE   10 (bases 1 to 935)
  AUTHORS   Zamore PD and Green MR.
  TITLE     Identification, purification, and biochemical characterization of
            U2 small nuclear ribonucleoprotein auxiliary factor
  JOURNAL   Proc Natl Acad Sci U S A 86 (23), 9243-9247 (1989)
   PUBMED   2531895
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC105905.1.
            
            On Aug 24, 2006 this sequence version replaced XM_001079206.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105905.1, CD372826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..935
                     /gene="U2af1"
                     /note="U2 small nuclear RNA auxiliary factor 1"
                     /db_xref="GeneID:687575"
                     /db_xref="RGD:1584190"
     exon            1..95
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..771
                     /gene="U2af1"
                     /codon_start=1
                     /product="splicing factor U2AF 35 kDa subunit"
                     /protein_id="NP_001037756.1"
                     /db_xref="GeneID:687575"
                     /db_xref="RGD:1584190"
                     /translation="MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ
                     TIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVC
                     DNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEM
                     GECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGG
                     GGGGRERDRRRSRDRERSGRF"
     exon            96..183
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            184..250
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            251..300
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            301..399
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            400..533
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            534..626
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
     exon            627..906
                     /gene="U2af1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcggcacag tcggcagcgt cggctgccgt gccggagtcg tccgctggaa aatggcggaa
       61 tacttggcct ccatcttcgg caccgagaaa gacaaagtca actgttcatt ttatttcaaa
      121 attggagcgt gtcgtcatgg agacagatgt tctcggttgc acaataaacc gacctttagc
      181 cagaccattg ccctcttgaa catttaccgt aaccctcaaa actcttccca gtctgctgac
      241 ggtttgcgct gtgcggtgag cgacgtggag atgcaggagc actatgacga gttctttgag
      301 gaagtcttca ctgagatgga agagaagtac ggggaggtcg aggagatgaa cgtctgtgac
      361 aacctaggag accacctggt ggggaatgtg tatgtcaagt tccgacgtga ggaggatgcg
      421 gagaaagcag tgatcgactt gaacaaccgc tggtttaatg ggcagccaat ccacgcagag
      481 ctgtccccag taactgactt cagggaagct tgctgccgcc agtatgaaat gggggagtgc
      541 acaagagggg gcttctgcaa cttcatgcat ctgaagccga tctcaagaga gctgcgacgg
      601 gagctgtatg ggcgccggcg caagaagcat agatccaggt cccgatccag ggagcggcgt
      661 tcccgatcga gagaccgtgg acgcggtggt ggaggtggag gtggaggcgg tggaggacgt
      721 gagcgtgaca ggaggcggtc aagagaccgg gagagatctg ggcgattctg agccgagccg
      781 tttttaccat gtgctagaca gtgttgtagt tggttggcaa gccagttcat agtgggattt
      841 tttttttaaa aaacaacaaa aaaaaacaaa gatgggtttc tgaataaaat ttgtagtgat
      901 acagtaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
