LOCUS       NM_001184712            2799 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus thioredoxin reductase 3 (Txnrd3), transcript
            variant 1, mRNA.
ACCESSION   NM_001184712
VERSION     NM_001184712.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Liu,A., Li,F., Xu,P., Chen,Y., Liang,X., Zheng,S., Meng,H., Zhu,Y.,
            Mo,J., Gong,C. and Zhou,J.C.
  TITLE     Gpx4, Selenov, and Txnrd3 Are Three Most Testis-Abundant
            Selenogenes Resistant to Dietary Selenium Concentrations and
            Actively Expressed During Reproductive Ages in Rats
  JOURNAL   Biol Trace Elem Res 201 (1), 250-259 (2023)
   PUBMED   35076866
  REMARK    GeneRIF: Gpx4, Selenov, and Txnrd3 Are Three Most Testis-Abundant
            Selenogenes Resistant to Dietary Selenium Concentrations and
            Actively Expressed During Reproductive Ages in Rats.
            Erratum:[Biol Trace Elem Res. 2022 Mar 1;:. PMID: 35233715]
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Hughes,D.J., Kunicka,T., Schomburg,L., Liska,V., Swan,N. and
            Soucek,P.
  TITLE     Expression of Selenoprotein Genes and Association with Selenium
            Status in Colorectal Adenoma and Colorectal Cancer
  JOURNAL   Nutrients 10 (11), 1812 (2018)
   PUBMED   30469315
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2799)
  AUTHORS   Cui,W., Dai,X., Marcho,C., Han,Z., Zhang,K., Tremblay,K.D. and
            Mager,J.
  TITLE     Towards Functional Annotation of the Preimplantation Transcriptome:
            An RNAi Screen in Mammalian Embryos
  JOURNAL   Sci Rep 6, 37396 (2016)
   PUBMED   27869233
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2799)
  AUTHORS   Gladyshev,V.N., Arner,E.S., Berry,M.J., Brigelius-Flohe,R.,
            Bruford,E.A., Burk,R.F., Carlson,B.A., Castellano,S., Chavatte,L.,
            Conrad,M., Copeland,P.R., Diamond,A.M., Driscoll,D.M., Ferreiro,A.,
            Flohe,L., Green,F.R., Guigo,R., Handy,D.E., Hatfield,D.L.,
            Hesketh,J., Hoffmann,P.R., Holmgren,A., Hondal,R.J., Howard,M.T.,
            Huang,K., Kim,H.Y., Kim,I.Y., Kohrle,J., Krol,A., Kryukov,G.V.,
            Lee,B.J., Lee,B.C., Lei,X.G., Liu,Q., Lescure,A., Lobanov,A.V.,
            Loscalzo,J., Maiorino,M., Mariotti,M., Sandeep Prabhu,K.,
            Rayman,M.P., Rozovsky,S., Salinas,G., Schmidt,E.E., Schomburg,L.,
            Schweizer,U., Simonovic,M., Sunde,R.A., Tsuji,P.A., Tweedie,S.,
            Ursini,F., Whanger,P.D. and Zhang,Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   5  (bases 1 to 2799)
  AUTHORS   Mariotti,M., Ridge,P.G., Zhang,Y., Lobanov,A.V., Pringle,T.H.,
            Guigo,R., Hatfield,D.L. and Gladyshev,V.N.
  TITLE     Composition and evolution of the vertebrate and mammalian
            selenoproteomes
  JOURNAL   PLoS One 7 (3), e33066 (2012)
   PUBMED   22479358
REFERENCE   6  (bases 1 to 2799)
  AUTHORS   Gerashchenko,M.V., Su,D. and Gladyshev,V.N.
  TITLE     CUG start codon generates thioredoxin/glutathione reductase
            isoforms in mouse testes
  JOURNAL   J Biol Chem 285 (7), 4595-4602 (2010)
   PUBMED   20018845
REFERENCE   7  (bases 1 to 2799)
  AUTHORS   Sun,Q.A., Kirnarsky,L., Sherman,S. and Gladyshev,V.N.
  TITLE     Selenoprotein oxidoreductase with specificity for thioredoxin and
            glutathione systems
  JOURNAL   Proc Natl Acad Sci U S A 98 (7), 3673-3678 (2001)
   PUBMED   11259642
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN936395.1, JAXUCZ010000004.1,
            CB585038.1, CK603679.1, BF564749.1, CB327915.1 and BG380508.1.
            
            [WARNING] On Jan 27, 2025 this sequence was replaced by
            NM_001184712.2.
            
            Summary: The protein encoded by this gene belongs to the pyridine
            nucleotide-disulfide oxidoreductase family, and is a member of the
            thioredoxin (Trx) system. Three thioredoxin reductase (TrxR)
            isozymes are found in mammals. TrxRs are selenocysteine-containing
            flavoenzymes, which reduce thioredoxins, as well as other
            substrates, and play a key role in redox homoeostasis. This gene
            encodes the third TrxR, which unlike the other two isozymes,
            contains an additional N-terminal glutaredoxin (Grx) domain, and
            shows highest expression in testis. The Grx domain allows this
            isozyme to participate in both Trx and glutathione systems. It
            functions as a homodimer containing FAD, and selenocysteine (Sec)
            at the active site. Sec is encoded by UGA codon that normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, the Sec insertion sequence
            (SECIS) element, which is necessary for the recognition of UGA as a
            Sec codon rather than as a stop signal. There is evidence for two
            isoforms resulting from the use of a non-AUG (CUG), and an in-frame
            downstream AUG as translation initiation codons (PMID:20018845).
            [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) contains two in-frame
            translation initiation codons: an upstream non-AUG (CUG) and a
            downstream AUG codon. The longer isoform (1) represented in this
            RefSeq results from the use of the CUG start codon.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript and
            the encoded protein are supported by orthologous mouse data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.16854.1,
                                           SRR26643294.18974.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon        :: inferred from conservation
            protein contains selenocysteine :: inferred from conservation
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-378               DN936395.1         52-429              c
            379-415             JAXUCZ010000004.1  123630146-123630182
            416-476             JAXUCZ010000004.1  123636688-123636748
            477-586             JAXUCZ010000004.1  123637671-123637780
            587-691             JAXUCZ010000004.1  123640241-123640345
            692-764             JAXUCZ010000004.1  123640554-123640626
            765-884             JAXUCZ010000004.1  123649198-123649317
            885-1027            JAXUCZ010000004.1  123656791-123656933
            1028-1604           CB585038.1         13-589
            1605-2014           CK603679.1         301-710
            2015-2186           BF564749.1         308-479
            2187-2768           CB327915.1         16-597              c
            2769-2799           BG380508.1         1-31                c
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..2799
                     /gene="Txnrd3"
                     /note="thioredoxin reductase 3"
                     /db_xref="GeneID:297437"
                     /db_xref="RGD:1308363"
     exon            1..415
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..2104
                     /gene="Txnrd3"
                     /EC_number="1.8.1.9"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     is encoded by transcript variant 1; non-AUG (CUG)
                     translation initiation codon"
                     /codon_start=1
                     /transl_except=(pos:2096..2098,aa:Sec)
                     /product="thioredoxin reductase 3 isoform 1"
                     /protein_id="NP_001171641.1"
                     /db_xref="GeneID:297437"
                     /db_xref="RGD:1308363"
                     /translation="MEKPPSPPPPPRAQTSPGQGKAGVVPNRRLGAVRGGLMSSPPGR
                     RARLTSPGTSRPPAEVREEVRRRLRDLIEGNRVMIFSKSYCPHSSRVKELFSSLGVNY
                     YILELDQVDDGANVQEVLTEISNQKTVPNIFVNKVHVGGCDRIFQAHQNGLLQKLLQD
                     DSTHDYDLIVIGGGSGGLSCAKEAANLGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCI
                     PKKLMHQAALLGHALQDARKYGWDYNQQVKHNWETMREAIQNHIGSLNWGYRVTLREK
                     GVTYVNSFGEFVELHKIKATNKKGQETFYTASKFVIATGERPRYLGIQGDKEYCITSD
                     DLFSLPYCPGNTLIVGASYVGLECAGFLAGLGLDVTVMVRSVLLRGFDQEMAEKVGSY
                     LEQQGVKFQRKFTPILVQQLEKGLPGRLKVVAKSTEGPETVEGTYNTVLLAIGRDSCT
                     RKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDVLEGKPELTPVAIQAGKLLAR
                     RLFGISLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEMYTKENLEVYHTFFWPLEWTV
                     AGRDNNTCYAKIICNKFDNDRVIGFHLLGPNAGEVTQGFAAAMKCGLTKQLLDDTIGI
                     HPTCGEVFTTMEITKSSGLDITQKGCUG"
     exon            416..476
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            477..586
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            587..691
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            692..764
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            765..884
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1027
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1143
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1369
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1462
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1539
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1696
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1804
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1900
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2035
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2783
                     /gene="Txnrd3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2241..2322
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Txnrd3"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
ORIGIN      
        1 gtggccccgt agcagtcccg tgcgcccgtg gccagtgcgc agcgttctca agttctcagc
       61 ggctttgctg gtgtcgtccc ctaggcggct gcccgcgact cggttcccca gcaggcccgg
      121 ctcggccggg agcgacaacg aagccctgga gaagccaccg tccccgccgc cgcctcctcg
      181 ggcgcaaacc tcgcctgggc aggggaaggc tggagtcgtc cccaaccgcc gcctgggtgc
      241 tgttcgtggc ggcctcatgt cgtcgccacc cggccgccgc gcccgcctga cgtcccccgg
      301 gaccagccgc ccgcccgctg aggtccgcga ggaggtgcgg cgccgcctgc gggatctcat
      361 cgagggcaac agggttatga tcttcagcaa gagttactgt ccacacagct cgcgggttaa
      421 ggaactcttt tcatccttgg gagtgaacta ttacatcctg gaacttgatc aagttgatga
      481 tggggccaat gttcaggaag tgctgacaga aatcagtaac cagaaaacgg tgcccaatat
      541 ttttgtgaat aaagtgcacg tgggtggatg tgaccggatt ttccaggcac atcagaatgg
      601 tttactgcag aagctccttc aggatgactc aactcatgat tatgatctca tcgtcatcgg
      661 cgggggctct ggcggcctct cttgtgccaa ggaagctgcc aacttgggaa agaaggtcat
      721 ggtgctagac tttgtggttc cctcgcctca gggcacgtcc tggggccttg gcggcacctg
      781 tgtgaatgta ggctgtatcc ctaagaagct gatgcatcag gcagccctcc tggggcatgc
      841 tctgcaagat gcgaggaaat atggctggga ttataaccag caggtgaagc acaactggga
      901 gaccatgagg gaagcgatcc agaaccacat tggctccttg aactggggct acagggtaac
      961 tcttcgggag aaaggcgtga cctatgtcaa ctccttcggg gagtttgtgg aactgcataa
     1021 aataaaggca accaataaga aagggcagga aactttttac accgcttcga agtttgtcat
     1081 agcaacaggg gaaaggccgc ggtacttggg aatccaggga gataaagagt actgtattac
     1141 cagcgacgac ctgttctctc tgccatactg cccagggaac acattaatcg ttggcgcctc
     1201 gtatgttggt ctggagtgtg caggcttttt ggctggcttg gggctagatg tgacagtcat
     1261 ggtacgctct gtccttcttc gtggctttga tcaagaaatg gcagagaaag tgggatccta
     1321 cctggaacaa caaggcgtca agttccaaag gaaattcacc cccattttgg ttcaacagtt
     1381 ggagaaaggc ttacccggaa gattgaaagt cgtggctaag tccactgaag gaccagagac
     1441 agtagaaggg acatacaaca cggttttgtt agcaattggt cgtgactcct gtacaaggaa
     1501 aatagggctg gagaagatcg gggtcaaaat caatgagaag aatggcaaaa taccagtaaa
     1561 tgatgtggaa cagaccaatg tgcctcatgt ttatgctatt ggggacgtac tggagggcaa
     1621 accagagctc acacctgttg ccatacaggc aggcaagctg ctagctcgaa gactctttgg
     1681 gatctcttta gaaaagtgtg attacattaa cgtcccaaca acggtgttca cacctctgga
     1741 atacggctgt tgtggactgt ctgaagagaa agccattgaa atgtatacga aggagaatct
     1801 ggaagtgtat cacaccttct tttggcctct tgagtggaca gttgctggca gagacaacaa
     1861 cacctgttat gcaaagataa tctgcaacaa atttgacaat gaccgtgtga taggatttca
     1921 cctcctgggg ccaaatgctg gtgaagtcac acaggggttt gcagctgcaa tgaaatgtgg
     1981 gcttacgaag cagctactgg atgacaccat tggaatccac cccacctgtg gtgaggtatt
     2041 tacaaccatg gaaatcacaa agtcctcagg gctggacatt actcagaaag gctgctgagg
     2101 ctagccctgc tgctgttggg ttccctgtca cattctcact ctgtacccat gacatctgct
     2161 tgggttctgg gcttccttct ggcgtagaga tgagaaggtg gcagaggacc agcagcagca
     2221 gcatctgtag tctcagctga tggcgtgcag cagccaggct gcatccttga cgccttggct
     2281 cggaacctgc agaggtgagc caaggccgac tcctgcacgt cagcctcgac tccccccaca
     2341 agcccttgga tggcacagtg agcccacccc cctttcccag ttgccagtgc tttggcccct
     2401 cactttgttt ccatgaagag attatctttt ttcagttgtg aacttgtctg taaagaggcc
     2461 tgtgtgccta gagtgcggcc cttacaggtc catggtgtct gaggaggcag gttagagcag
     2521 ttggctgcac aggcctcctg ctaataggcc atatgcactg tatagccatt taaaggacta
     2581 tttaacctgc tgtcagaaac ctgactcata ccgcactcct tgcacagctt ctaggggaga
     2641 accttgaagc cattctcctc ctagcttata gtctaacaga gcatcacata gaagagaaaa
     2701 gtcctgcatt gctgccgttt tcccatgtct atcttcagat attaactatt aaaaaatacg
     2761 taactacagt ttgtgtgatc taaaaaaaaa aaaaaaaaa
//
