LOCUS       XM_008760431            1126 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus twinkle mtDNA helicase (Twnk),
            transcript variant X2, mRNA.
ACCESSION   XM_008760431
VERSION     XM_008760431.3
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051336.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_008760431.4.
            
            On Jan 21, 2021 this sequence version replaced XM_008760431.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..1126
                     /gene="Twnk"
                     /gene_synonym="Peo1"
                     /note="twinkle mtDNA helicase; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 6
                     ESTs, 1 Protein, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 53 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:309441"
                     /db_xref="RGD:1307884"
     CDS             252..947
                     /gene="Twnk"
                     /gene_synonym="Peo1"
                     /codon_start=1
                     /product="twinkle mtDNA helicase isoform X2"
                     /protein_id="XP_008758653.1"
                     /db_xref="GeneID:309441"
                     /db_xref="RGD:1307884"
                     /translation="MLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVI
                     DTMQHAVYVYDVCHVVIDNLQFMMGHEQLSTDRIAAQDYIVGAFRKFATDNSCHVTLV
                     IHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDV
                     GVFPLEFNKSSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGSAHQNSETCIGQDP
                     CQPDTSPSSRHKN"
ORIGIN      
        1 gcgagcggcg cgtgcgggca acccacgttt tggacagaac actagagagg agagtggtga
       61 cgcgaagctg ggggcgggag cgtggagctt ctgaagccga gggtagagcc ctggagcctg
      121 taagggagcc ggggccaaca ggcagtggca agacaacatt catcagtgag tatgccctgg
      181 acttgtgtac ccagggagtg aacacactgt ggggtagctt tgagatcagc aatgtgagac
      241 tagcccgggt catgctgact cagtttgctg tgacacggct agaagaacaa ctggacaagt
      301 atgaagagtg ggcagaccgt tttgaggacc tgcctctcta tttcatgact ttccacggac
      361 aacagagcat caggtctgtc atagacacaa tgcaacatgc tgtctacgtc tatgacgttt
      421 gtcatgtagt catcgacaac ttgcagttca tgatgggtca tgagcagctc tctactgaca
      481 ggatcgcagc tcaagactac attgttggag cctttcggaa gtttgccaca gacaatagct
      541 gccacgtgac tctggttatt cacccacgga aagaagatga cgacaaggaa ctacagacag
      601 catccatttt tggctcagcc aaagccagcc aagaagcaga caatgttctg atcttgcagg
      661 acaggaagct ggtgactggg cctgggaaac ggtatctgca ggtgtccaaa aatcgctttg
      721 atggagacgt aggcgtcttc ccacttgagt tcaacaagag ttcccttacc ttctccatcc
      781 cacctaagag caaagcccga ctcaagaaga ttaaggatga caatggacta gtggccaaga
      841 agtcctcctc tggaaaaaaa gggtctgcgc accagaactc tgagacttgc ataggccagg
      901 acccctgcca gccagatacc tccccgtcct caaggcataa gaattgatct gaggtgctta
      961 tcctgtgctg tcagagcctc aaagctggac catggagaga agctgtgagc ctgcagtgcc
     1021 tgcccccttg atgccgattc cactgtgtgc tgctgccttg gacagcggga gctaggactc
     1081 ttggtggagc cttttccagt tgaatgaagc atttctatgg cctctg
//
