LOCUS       NM_001033901            2699 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus terminal uridylyl transferase 1, U6
            snRNA-specific (Tut1), mRNA.
ACCESSION   NM_001033901 XM_574615
VERSION     NM_001033901.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Sudheesh,A.P., Mohan,N., Francis,N., Laishram,R.S. and
            Anderson,R.A.
  TITLE     Star-PAP controlled alternative polyadenylation coupled poly(A)
            tail length regulates protein expression in hypertrophic heart
  JOURNAL   Nucleic Acids Res 47 (20), 10771-10787 (2019)
   PUBMED   31598705
  REMARK    GeneRIF: The loss of NQO1 during hypertrophy was rescued by ectopic
            expression of the distal- but not the proximal- or middle-specific
            NQO1 mRNA isoforms in the presence of Star-PAP expression, and
            reverses molecular events of hypertrophy in cardiomyocytes.
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Yamashita,S., Takagi,Y., Nagaike,T. and Tomita,K.
  TITLE     Crystal structures of U6 snRNA-specific terminal
            uridylyltransferase
  JOURNAL   Nat Commun 8, 15788 (2017)
   PUBMED   28589955
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2699)
  AUTHORS   Laishram,R.S. and Anderson,R.A.
  TITLE     The poly A polymerase Star-PAP controls 3'-end cleavage by
            promoting CPSF interaction and specificity toward the pre-mRNA
  JOURNAL   EMBO J 29 (24), 4132-4145 (2010)
   PUBMED   21102410
REFERENCE   4  (bases 1 to 2699)
  AUTHORS   Gonzales,M.L., Mellman,D.L. and Anderson,R.A.
  TITLE     CKIalpha is associated with and phosphorylates star-PAP and is also
            required for expression of select star-PAP target messenger RNAs
  JOURNAL   J Biol Chem 283 (18), 12665-12673 (2008)
   PUBMED   18305108
REFERENCE   5  (bases 1 to 2699)
  AUTHORS   Mellman,D.L., Gonzales,M.L., Song,C., Barlow,C.A., Wang,P.,
            Kendziorski,C. and Anderson,R.A.
  TITLE     A PtdIns4,5P2-regulated nuclear poly(A) polymerase controls
            expression of select mRNAs
  JOURNAL   Nature 451 (7181), 1013-1017 (2008)
   PUBMED   18288197
REFERENCE   6  (bases 1 to 2699)
  AUTHORS   Mullen,T.E. and Marzluff,W.F.
  TITLE     Degradation of histone mRNA requires oligouridylation followed by
            decapping and simultaneous degradation of the mRNA both 5' to 3'
            and 3' to 5'
  JOURNAL   Genes Dev 22 (1), 50-65 (2008)
   PUBMED   18172165
REFERENCE   7  (bases 1 to 2699)
  AUTHORS   Trippe,R., Guschina,E., Hossbach,M., Urlaub,H., Luhrmann,R. and
            Benecke,B.J.
  TITLE     Identification, cloning, and functional analysis of the human U6
            snRNA-specific terminal uridylyl transferase
  JOURNAL   RNA 12 (8), 1494-1504 (2006)
   PUBMED   16790842
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC104695.1.
            
            On Sep 30, 2005 this sequence version replaced XM_574615.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC104695.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..2699
                     /gene="Tut1"
                     /note="terminal uridylyl transferase 1, U6 snRNA-specific"
                     /db_xref="GeneID:499314"
                     /db_xref="RGD:1561043"
     exon            1..94
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..2613
                     /gene="Tut1"
                     /EC_number="2.7.7.19"
                     /EC_number="2.7.7.52"
                     /note="U6 snRNA-specific terminal uridylyltransferase 1;
                     U6-TUTase; RNA-binding protein 21; RNA-binding motif
                     protein 21; star-PAP"
                     /codon_start=1
                     /product="speckle targeted PIP5K1A-regulated poly(A)
                     polymerase"
                     /protein_id="NP_001029073.1"
                     /db_xref="GeneID:499314"
                     /db_xref="RGD:1561043"
                     /translation="MAAVDSDVVSLPRGRFRCCLCDVTTANRPSLDAHLKGRKHRDLV
                     QLRATRKAQGLRSVFVSGFPRDVGSAQLSEYFQTFGPVANIVMDKDKGVFAIVEMGDI
                     SAREAVLSQPKHSLGGHTLRVRPREQKEFQSPASKSPKGVDSNSHQLAQALAEAADVG
                     AQMVKLVELRELSEAERQLRTLVVALMQEVFTEFFPGCVVHPFGSSVNSFDVHGCDLD
                     LFLDLGDMEEPQPDPQTPKLPEASSLDSTLASSLDPQVLACTPASLDSLSPTSLQDSE
                     ALDFETPSSLAPQTPDSALGSDTVTSPQSLPPVSPLEEDRGEGKHRKELELAEASKDE
                     KEEATAVLELVGSILRGCVPGVYRVQTVPSARRPVVKFCHRPSGLHGDISLSNRLALY
                     NSRFLNLCSEMDSRVRPLVYTLRCWAQHNGLSGGGPLLNNYALTLLVIYFLQTRDPPV
                     LPTVAQLTQRSGEGEQVEVDGWDCSFPKDASRLEPSTNVEPLSSLLAQFFSCVSCWDL
                     SGSLLSLREGQALMVAGGLPSDLWEGLRLGPMNLQDPFDLSHNVAANVTSRVAKRLQS
                     SCGAAASYCRSLQYQQRSSRGRDWGLLPLLQPSSPSSLLSAKLIPLPSAPFPQIITAL
                     VSVLREALGCHIEQGTKRRRSEGARSKDSPLGGANKRPRLSGQEKSCEEGKEEPQGCA
                     GDHSENEVEEMVIELRETPQDWALLHCGPPGELPLMTAKCLDKTAEQNPMEPEGAGEG
                     SPGETEKEASHPSSVSWRCALWHQIWQGRRRARRRFQQQTKEEGRGGPSTGAEWLAVE
                     ARVTQELKGPKSEQQRLQGEPLLTFVASASQAEQTLTVAPLQDPQGLFPGLHHFLQVF
                     IPQALKNLLK"
     exon            95..285
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            286..601
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..702
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..1175
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1285
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1396
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1489
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..2661
                     /gene="Tut1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggagagtgg ctatggcggc ggtggattcg gatgttgtat cgctgccgcg tggccgcttt
       61 cgctgctgcc tctgcgatgt tactacagcc aaccgaccca gcctagatgc ccacttgaaa
      121 ggccggaagc accgggatct ggtacaacta cgagctacca gaaaggccca gggacttcga
      181 agtgtgtttg tcagtggctt tcccagggat gtaggctctg ctcagctctc tgaatacttc
      241 cagacatttg gccctgtggc caatattgtt atggacaagg acaagggggt gtttgccatc
      301 gtggagatgg gagacataag tgctcgggag gctgtcttat ctcagcccaa gcacagcctt
      361 gggggacaca cactgcgtgt ccggccaagg gagcagaagg agttccagag cccagcttcc
      421 aagtctccca aaggagtgga ctcaaatagt caccagctgg cccaagcact ggctgaggcc
      481 gcagatgtag gggcacagat ggtgaagctt gtggaactga gggagttgtc tgaggctgag
      541 cggcagcttc ggacccttgt tgtggccctg atgcaggagg tcttcacaga gttctttcct
      601 ggctgtgtgg tccatccttt tggctcttct gtcaatagct ttgatgttca tggctgtgat
      661 ctcgacctct tcttggactt gggagatatg gaagagcccc agccagaccc acagactcca
      721 aagcttccag aggcttcgtc cttggactca acccttgctt cttccctgga tcctcaagtg
      781 ctggcctgca ccccagcttc tctagactca ctgtctccaa catctctcca agattctgaa
      841 gccctggact ttgaaacccc ttcttctctg gcaccacaga ccccagactc tgctttgggc
      901 tctgacactg tcacctctcc ccagtccctg cctccagttt ccccactgga ggaggacagg
      961 ggagagggga aacacaggaa ggagctagaa ttagcagaag cctcaaagga cgagaaggag
     1021 gaggcaacag cagtgttaga gctggtggga tctattctcc gtggctgtgt ccctggagtg
     1081 taccgagtcc aaactgtgcc ctctgccagg cgtcctgtgg tcaagttctg tcatcggcct
     1141 tcaggtcttc atggagacat ctccctcagt aaccggttgg ccttgtataa ttcccgcttc
     1201 ctgaacctat gctctgagat ggatagtcga gtccggcccc ttgtgtatac cttacgctgc
     1261 tgggctcagc ataatgggct gtcagggggc ggcccccttc tcaataacta cgccttgacg
     1321 ttgttagtga tctacttcct tcagaccaga gaccctccag tgcttcctac tgtggcccag
     1381 cttacgcaga gatcaggtga aggggagcag gtagaagttg atggctggga ctgtagcttc
     1441 cctaaggatg cctcaagact ggagcccagc accaacgtag agcctctcag ttccctgctg
     1501 gcccagttct tctcctgcgt gtcttgctgg gatctttctg gctccctgct gtccctgcgg
     1561 gaaggtcagg cactgatggt ggcagggggc ctgccttctg atctctggga agggctgcgc
     1621 cttggcccca tgaatctcca ggaccccttt gatctgagtc acaatgttgc agccaatgtg
     1681 accagccgcg tggctaagcg tctgcagagc agttgtggag cagcagctag ttactgccgg
     1741 agtctccagt accagcagcg ctcctcccgg ggccgggact ggggactgct cccacttttg
     1801 cagcccagct cccctagctc cctgctctct gccaagctta tccccttacc ctctgccccc
     1861 tttccacaga tcattacagc tttggtgagt gtgttaaggg aagcactagg atgccatata
     1921 gaacagggaa ccaagagacg acggtcggaa ggtgccagat ctaaagactc tcccctggga
     1981 ggggcaaaca aaagaccaag acttagtggg caagaaaaga gctgtgagga agggaaagag
     2041 gagccacagg gatgtgcagg ggaccacagt gaaaatgagg tagaagaaat ggtaatagaa
     2101 ttgcgagaga cacctcagga ctgggccttg ttgcactgtg gaccaccagg ggagttgccc
     2161 ctgatgactg caaaatgtct agacaagact gctgagcaga atcccatgga acctgagggg
     2221 gctggagaag ggtcccccgg tgagacagag aaggaggctt cacacccgtc atcagtgagt
     2281 tggcgctgtg ccttgtggca ccaaatatgg caggggaggc ggcgtgcccg gagacgattt
     2341 cagcagcaaa ccaaggaaga aggcagagga ggtcccagca ccggagcaga gtggctggca
     2401 gtggaagcac gagtaaccca ggaactgaag ggaccaaaga gtgagcagca gaggctacag
     2461 ggggagcccc tcctcacctt tgtggcatcg gcctcccaag ctgagcagac actcactgtg
     2521 gcacccctcc aggatcccca aggcctgttc cctggtcttc atcattttct acaggttttt
     2581 atccctcaag cacttaaaaa tctcctcaag tgaggagcca agcctcaaaa gcaataaagc
     2641 tgctagttta tttcaaacag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
