LOCUS       NM_001413946            1243 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus testis-specific serine kinase 4 (Tssk4),
            transcript variant 3, mRNA.
ACCESSION   NM_001413946 XM_006251938
VERSION     NM_001413946.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Fu,G., Wei,Y., Wang,X. and Yu,L.
  TITLE     Phosphorylated testis-specific serine/threonine kinase 4 may
            phosphorylate Crem at Ser-117
  JOURNAL   Biosci Biotechnol Biochem 80 (6), 1088-1094 (2016)
   PUBMED   26940607
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Wang,X., Li,H., Fu,G., Wang,Y., Du,S., Yu,L., Wei,Y. and Chen,S.
  TITLE     Testis-specific serine/threonine protein kinase 4 (Tssk4)
            phosphorylates Odf2 at Ser-76
  JOURNAL   Sci Rep 6, 22861 (2016)
   PUBMED   26961893
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1243)
  AUTHORS   Wang,X., Wei,Y., Fu,G., Li,H., Saiyin,H., Lin,G., Wang,Z., Chen,S.
            and Yu,L.
  TITLE     Tssk4 is essential for maintaining the structural integrity of
            sperm flagellum
  JOURNAL   Mol Hum Reprod 21 (2), 136-145 (2015)
   PUBMED   25361759
REFERENCE   4  (bases 1 to 1243)
  AUTHORS   Jha,K.N., Coleman,A.R., Wong,L., Salicioni,A.M., Howcroft,E. and
            Johnson,G.R.
  TITLE     Heat shock protein 90 functions to stabilize and activate the
            testis-specific serine/threonine kinases, a family of kinases
            essential for male fertility
  JOURNAL   J Biol Chem 288 (23), 16308-16320 (2013)
   PUBMED   23599433
REFERENCE   5  (bases 1 to 1243)
  AUTHORS   Wei,Y., Wang,X., Fu,G. and Yu,L.
  TITLE     Testis specific serine/threonine kinase 4 (Tssk4) maintains its
            kinase activity by phosphorylating itself at Thr-197
  JOURNAL   Mol Biol Rep 40 (1), 439-447 (2013)
   PUBMED   23054012
REFERENCE   6  (bases 1 to 1243)
  AUTHORS   Li,Y., Sosnik,J., Brassard,L., Reese,M., Spiridonov,N.A.,
            Bates,T.C., Johnson,G.R., Anguita,J., Visconti,P.E. and
            Salicioni,A.M.
  TITLE     Expression and localization of five members of the testis-specific
            serine kinase (Tssk) family in mouse and human sperm and testis
  JOURNAL   Mol Hum Reprod 17 (1), 42-56 (2011)
   PUBMED   20729278
REFERENCE   7  (bases 1 to 1243)
  AUTHORS   Wei,Y., Fu,G., Hu,H., Lin,G., Yang,J., Guo,J., Zhu,Q. and Yu,L.
  TITLE     Isolation and characterization of mouse testis specific
            serine/threonine kinase 5 possessing four alternatively spliced
            variants
  JOURNAL   J Biochem Mol Biol 40 (5), 749-756 (2007)
   PUBMED   17927909
REFERENCE   8  (bases 1 to 1243)
  AUTHORS   Chen,X., Lin,G., Wei,Y., Hexige,S., Niu,Y., Liu,L., Yang,C. and
            Yu,L.
  TITLE     TSSK5, a novel member of the testis-specific serine/threonine
            kinase family, phosphorylates CREB at Ser-133, and stimulates the
            CRE/CREB responsive pathway
  JOURNAL   Biochem Biophys Res Commun 333 (3), 742-749 (2005)
   PUBMED   15964553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Nov 10, 2022 this sequence version replaced XM_006251938.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643293.59630.1,
                                           SRR26360181.925954.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-357               JAXUCZ010000015.1  33111734-33112090
            358-572             JAXUCZ010000015.1  33112426-33112640
            573-966             JAXUCZ010000015.1  33112994-33113387
            967-1243            JAXUCZ010000015.1  33113807-33114083
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p13"
     gene            1..1243
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /note="testis-specific serine kinase 4"
                     /db_xref="GeneID:290229"
                     /db_xref="RGD:1560814"
     exon            1..357
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1152
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 3;
                     testis-specific serine/threonine-protein kinase 4"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     4 isoform 2"
                     /protein_id="NP_001400875.1"
                     /db_xref="GeneID:290229"
                     /db_xref="RGD:1560814"
                     /translation="MGKGDTSETASATPAYRSVMEEYGYEVGKVIGHGSYGTVYEAYY
                     TKQKVMVAVKIISKKKASEDYLNKFLPREIQVMKVLRHKYLINFYQAIETTSRVYIIL
                     ELAQGGDVLEWIQRYGACSETLAGKWFSQMALGIAYLHSKGIVHRLTPSLSAAGRDLK
                     LENLLLDKRENVKISDFGFAKMVPSSQPVRSSPSYRQMNCLSHLSQTYCGSFAYACPE
                     ILLGLPYNPFLSDTWSMGVILYTLVVARLPFDDTNLKKLLRETQKEVTFPANLTISQE
                     CKNLILQLLRPATKRATILDVLRDPWMLKFQSEQPSNEIRLLEAMYQPASTTANRHQS
                     LEITT"
     exon            358..572
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /inference="alignment:Splign:2.1.0"
     exon            573..966
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1243
                     /gene="Tssk4"
                     /gene_synonym="RGD1560814"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agacttgtgt gactgggtaa acactgggac actaacttca cttcagtacc ctcccttcag
       61 aagctcaagg tggcttctcg gtagacagag gcttctaagt atcaaagctc cccactgcgc
      121 aagcccaaca tcatggggaa gggagacacc tcggaaacag catcagccac tccagcctat
      181 cgctctgtca tggaggagta tggctatgag gtgggcaagg tcattggcca tggctcctat
      241 ggaactgtct atgaggctta ctacaccaag cagaaggtca tggtggctgt caagatcatc
      301 tcaaagaaga aggcctctga agactacctt aacaagttcc taccacgtga gatacaggta
      361 atgaaagtct tacggcacaa gtacctcatc aacttctatc aggccattga gaccacgtcc
      421 cgagtgtaca tcattttgga gctggctcag ggcggtgatg tcttggaatg gatccaacgc
      481 tatggggcct gctctgagac ccttgctggc aagtggttct cccagatggc cttgggcatc
      541 gcttacctgc acagcaaggg catcgtgcac cgcctgaccc ccagcctttc tgctgctggt
      601 agggacttaa agttggagaa cctgttgctg gacaagcggg agaatgtgaa gatatcggat
      661 tttggcttcg ccaagatggt gccttctagc cagcctgtgc gtagtagccc ttcttaccgc
      721 caaatgaact gcctttccca cctcagccag acctactgtg gcagctttgc ttacgcctgc
      781 ccggagatct tgctaggctt gccttacaac cctttcctgt ctgacacctg gagcatgggc
      841 gtcatcctct acaccctagt ggtcgcacgg ctgccttttg atgacaccaa tctcaagaag
      901 ctgctgagag aaacccagaa ggaggtcact ttcccagcta atctcaccat ctcccaggag
      961 tgcaagaacc tgatcctcca gttgctacgc ccagctacca agcgtgccac catcctagat
     1021 gtcctcaggg acccctggat gctcaagttc cagtctgagc aaccctcgaa cgaaatcagg
     1081 ctgctggagg ccatgtatca acccgccagc accaccgcta atcgccacca atccttggaa
     1141 atcacaactt gaaagtggct gaggcaggag gttaaggaaa aaagcaatag agactggggg
     1201 gaaaatcttt tctataaaat aaacctaatt agtttcatca tga
//
