LOCUS       NM_133526               1166 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus tetraspanin 8 (Tspan8), mRNA.
ACCESSION   NM_133526
VERSION     NM_133526.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Yildirim,H., Efe Daskan,B., Aksoz,E., Sen,F. and Celebi,M.
  TITLE     Regional expression differences of SERT and TSPAN8 in hippocampus,
            cerebellum and cortex of wild-type young, adult and middle-aged
            rats
  JOURNAL   Gene 885, 147706 (2023)
   PUBMED   37572802
  REMARK    GeneRIF: Regional expression differences of SERT and TSPAN8 in
            hippocampus, cerebellum and cortex of wild-type young, adult and
            middle-aged rats.
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Pradhan,B.S., Bhattacharya,I., Sarkar,R. and Majumdar,S.S.
  TITLE     Pubertal down-regulation of Tetraspanin 8 in testicular Sertoli
            cells is crucial for male fertility
  JOURNAL   Mol Hum Reprod 26 (10), 760-772 (2020)
   PUBMED   32687199
  REMARK    GeneRIF: Pubertal down-regulation of Tetraspanin 8 in testicular
            Sertoli cells is crucial for male fertility.
REFERENCE   3  (bases 1 to 1166)
  AUTHORS   Mu,W., Provaznik,J., Hackert,T. and Zoller,M.
  TITLE     Tspan8-Tumor Extracellular Vesicle-Induced Endothelial Cell and
            Fibroblast Remodeling Relies on the Target Cell-Selective Response
  JOURNAL   Cells 9 (2), 319 (2020)
   PUBMED   32013145
  REMARK    GeneRIF: Tspan8-Tumor Extracellular Vesicle-Induced Endothelial
            Cell and Fibroblast Remodeling Relies on the Target Cell-Selective
            Response.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1166)
  AUTHORS   Voglstaetter,M., Thomsen,A.R., Nouvel,J., Koch,A., Jank,P.,
            Navarro,E.G., Gainey-Schleicher,T., Khanduri,R., Gross,A.,
            Rossner,F., Blaue,C., Franz,C.M., Veil,M., Puetz,G., Hippe,A.,
            Dindorf,J., Kashef,J., Thiele,W., Homey,B., Greco,C., Boucheix,C.,
            Baur,A., Erbes,T., Waller,C.F., Follo,M., Hossein,G., Sers,C.,
            Sleeman,J. and Nazarenko,I.
  TITLE     Tspan8 is expressed in breast cancer and regulates
            E-cadherin/catenin signalling and metastasis accompanied by
            increased circulating extracellular vesicles
  JOURNAL   J Pathol 248 (4), 421-437 (2019)
   PUBMED   30982971
  REMARK    GeneRIF: Findings show the presence of Tspan8 in breast cancer
            primary lesion and metastases and indicate its role as a regulator
            of cell behaviour and extracellular vesicle release in breast
            cancer. Tspan8 and p120-catenin were co-immunoprecipitated,
            indicating that they may interact with each other.
REFERENCE   5  (bases 1 to 1166)
  AUTHORS   Mutoji,K., Singh,A., Nguyen,T., Gildersleeve,H., Kaucher,A.V.,
            Oatley,M.J., Oatley,J.M., Velte,E.K., Geyer,C.B., Cheng,K.,
            McCarrey,J.R. and Hermann,B.P.
  TITLE     TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the
            Prepubertal Mouse Testis
  JOURNAL   Biol Reprod 95 (6), 117 (2016)
   PUBMED   27733379
REFERENCE   6  (bases 1 to 1166)
  AUTHORS   Yue,S., Mu,W. and Zoller,M.
  TITLE     Tspan8 and CD151 promote metastasis by distinct mechanisms
  JOURNAL   Eur J Cancer 49 (13), 2934-2948 (2013)
   PUBMED   23683890
  REMARK    GeneRIF: CD151 and Tspan8 coordinately promote metastasis, where
            Tspan8 overrides the adhesive features of CD151 by recruiting
            integrins out of adhesion into motility promoting complexes.
REFERENCE   7  (bases 1 to 1166)
  AUTHORS   Bubenek,S., Nastase,A., Niculescu,A.M., Baila,S., Herlea,V.,
            Lazar,V., Paslaru,L., Botezatu,A., Tomescu,D., Popescu,I. and
            Dima,S.
  TITLE     Assessment of gene expression profiles in peripheral occlusive
            arterial disease
  JOURNAL   Can J Cardiol 28 (6), 712-720 (2012)
   PUBMED   22721676
REFERENCE   8  (bases 1 to 1166)
  AUTHORS   Nazarenko,I., Rana,S., Baumann,A., McAlear,J., Hellwig,A.,
            Trendelenburg,M., Lochnit,G., Preissner,K.T. and Zoller,M.
  TITLE     Cell surface tetraspanin Tspan8 contributes to molecular pathways
            of exosome-induced endothelial cell activation
  JOURNAL   Cancer Res 70 (4), 1668-1678 (2010)
   PUBMED   20124479
  REMARK    GeneRIF: Tspan8 contributes to molecular pathways of
            exosome-induced endothelial cell activation.
            Erratum:[Cancer Res.2010 Aug 15;70(16):6683]
REFERENCE   9  (bases 1 to 1166)
  AUTHORS   Claas,C., Wahl,J., Orlicky,D.J., Karaduman,H., Schnolzer,M.,
            Kempf,T. and Zoller,M.
  TITLE     The tetraspanin D6.1A and its molecular partners on rat carcinoma
            cells
  JOURNAL   Biochem J 389 (Pt 1), 99-110 (2005)
   PUBMED   15725074
  REMARK    GeneRIF: The molecular interactions of D6.1A with multiple proteins
            in rat tumor cell lines are reported.
REFERENCE   10 (bases 1 to 1166)
  AUTHORS   Claas,C., Seiter,S., Claas,A., Savelyeva,L., Schwab,M. and
            Zoller,M.
  TITLE     Association between the rat homologue of CO-029, a
            metastasis-associated tetraspanin molecule and consumption
            coagulopathy
  JOURNAL   J Cell Biol 141 (1), 267-280 (1998)
   PUBMED   9531564
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 26, 2020 this sequence version replaced NM_133526.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y13275.2, FQ227464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-290               JAXUCZ010000007.1  53331187-53331476
            291-353             JAXUCZ010000007.1  53340380-53340442
            354-491             JAXUCZ010000007.1  53345440-53345577
            492-566             JAXUCZ010000007.1  53347423-53347497
            567-674             JAXUCZ010000007.1  53347604-53347711
            675-800             JAXUCZ010000007.1  53357185-53357310
            801-884             JAXUCZ010000007.1  53359412-53359495
            885-1166            JAXUCZ010000007.1  53365121-53365402
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..1166
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /note="tetraspanin 8"
                     /db_xref="GeneID:171048"
                     /db_xref="RGD:621783"
     exon            1..290
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..938
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /note="D6.1A; transmembrane 4 superfamily member 3"
                     /codon_start=1
                     /product="tetraspanin-8"
                     /protein_id="NP_598210.1"
                     /db_xref="GeneID:171048"
                     /db_xref="RGD:621783"
                     /translation="MAGVSGCLKYSMFFFNFLFWVCGTLILGLAIWLRVSKDGKEIIT
                     SGDNGTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILLLQVAA
                     GILGATFKSESSRILNETLYENAKLLSETSNEAKEVQKAMIAFQSEFKCCGLRFGAAD
                     WGKNFPDAKESCQCTGSDCESYNGENVYRTTCLSLIKELVEKNIIIVIGIAFGLAVIE
                     ILGLVFSMVLYCQIGSK"
     exon            291..353
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            354..491
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            492..566
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            567..674
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            675..800
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            801..884
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1166
                     /gene="Tspan8"
                     /gene_synonym="CO-29; Tm4sf3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acttgcctga agataacgtt tgggatattt aaaggtgtgg gttaattttt tatccatttt
       61 agtaactggg tttttttttt tttctttccg actacttcct tgctttctat tgctacccga
      121 agctgcaggc acacggatct ctgcaggcaa aatccctcag agccttcgca ggacagcctg
      181 gtaacgactg gttcgtccta ctgtaactgg gtggctgccc cttctccgaa atggcaggtg
      241 tcagtggctg tttaaagtat tcgatgtttt tcttcaactt cttgttctgg gtatgtggta
      301 cactgatcct gggtctagcc atatggttga gagtaagcaa agacggcaaa gagattatca
      361 catccggtga caatggcacg aaccccttca ttgctgtgaa tatcttgatt gcagttgggt
      421 ccatcatcat ggttctgggt ttcctgggat gctgcggagc tgtgaaagaa agtcgctgca
      481 tgcttctctt gttttttatc ggcttgctcc tgattctgct tctgcaagta gccgcaggta
      541 tcctaggagc tactttcaaa tctgaatcta gccgcattct gaatgaaacg ctctatgaaa
      601 atgccaagct tttgtcagaa acaagcaatg aggcaaaaga agtacagaag gccatgattg
      661 catttcagtc agagtttaag tgctgtggct tgagatttgg agctgctgac tggggaaaaa
      721 attttccaga tgcgaaagag tcatgccaat gtacaggatc tgattgtgaa agttataatg
      781 gagagaatgt ctatcgaacg acctgtcttt ctctgataaa agagctggtt gaaaaaaaca
      841 ttattattgt cattggaatt gcctttggac tggcagttat cgagattctt ggtttggtgt
      901 tttctatggt gctctactgc caaattggaa gcaaatgaat ctgcggactt cctcagtcgt
      961 tatagacacc atttaagttt ttgctttggc ttcgtaactt taaatactcg agtactgtac
     1021 ataccaggag cagatgtctt cttgatgagc tctcacttag gcagactaca ggtatcttca
     1081 catgtactca gctcatttaa ctgtgaagga cataataaaa gtggtcgtgt attttttttt
     1141 ctcacaataa aaagtgttcg tgctga
//
