LOCUS       NM_001009965            2574 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku),
            mRNA.
ACCESSION   NM_001009965 XM_218945
VERSION     NM_001009965.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Henke,A., Grace,O.C., Ashley,G.R., Stewart,G.D., Riddick,A.C.,
            Yeun,H., O'Donnell,M., Anderson,R.A. and Thomson,A.A.
  TITLE     Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and
            Tsukushi in developing prostate and decreased levels of decorin in
            prostate cancer
  JOURNAL   PLoS One 7 (8), e42516 (2012)
   PUBMED   22880013
  REMARK    GeneRIF: The spatio-temporal expression of Decorin, Semaphorin6D,
            SPARC, Spry-1 and Tsukushi in developing prostate is described and
            observed similar mesenchymal expression patterns in rat and human.
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Ohta,K., Ito,A., Kuriyama,S., Lupo,G., Kosaka,M., Ohnuma,S.,
            Nakagawa,S. and Tanaka,H.
  TITLE     Tsukushi functions as a Wnt signaling inhibitor by competing with
            Wnt2b for binding to transmembrane protein Frizzled4
  JOURNAL   Proc Natl Acad Sci U S A 108 (36), 14962-14967 (2011)
   PUBMED   21856951
REFERENCE   3  (bases 1 to 2574)
  AUTHORS   Ito,A., Shinmyo,Y., Abe,T., Oshima,N., Tanaka,H. and Ohta,K.
  TITLE     Tsukushi is required for anterior commissure formation in mouse
            brain
  JOURNAL   Biochem Biophys Res Commun 402 (4), 813-818 (2010)
   PUBMED   21055390
REFERENCE   4  (bases 1 to 2574)
  AUTHORS   Coffy,S., Decaux,J.F., Girard,J., de Keyzer,Y. and Asfari,M.
  TITLE     Identification of a novel rat hepatic gene induced early by
            insulin, independently of glucose
  JOURNAL   Biochem J 385 (Pt 1), 165-171 (2005)
   PUBMED   15344907
  REMARK    GeneRIF: Tissue distribution of EIIH in adult rats revealed a
            pattern of expression mainly in the liver, intestine and islets of
            Langerhans, closely following that of the Glut2 (glucose
            transporter 2), suggesting that it may play a role in carbohydrate
            metabolism
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001009965.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100644.1, SRR26360186.1041626.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               JAXUCZ010000001.1  162082635-162082768 c
            135-2574            JAXUCZ010000001.1  162071459-162073898 c
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..2574
                     /gene="Tsku"
                     /gene_synonym="Eiih"
                     /note="tsukushi, small leucine rich proteoglycan"
                     /db_xref="GeneID:308843"
                     /db_xref="RGD:1359311"
     exon            1..134
                     /gene="Tsku"
                     /gene_synonym="Eiih"
                     /inference="alignment:Splign:2.1.0"
     exon            135..2574
                     /gene="Tsku"
                     /gene_synonym="Eiih"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1204
                     /gene="Tsku"
                     /gene_synonym="Eiih"
                     /note="leucine-rich repeat-containing protein 54; early
                     insulin-induced hepatic gene protein; hepatic protein
                     EIIH; tsukushin"
                     /codon_start=1
                     /product="tsukushi precursor"
                     /protein_id="NP_001009965.2"
                     /db_xref="GeneID:308843"
                     /db_xref="RGD:1359311"
                     /translation="MLCTLFLLLLALGTVQTTRPCFPGCQCEEETFGLFDSFSLIRVD
                     CSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLGGPGYTTLAGLDLSHNLLTSITP
                     TAFSRLRYLESLDLSHNGLAALPAEVFTSSPLSDINLSHNRLREVSISAFTTHSQGRA
                     LHVDLSHNLIHRLLPYPARASLSAPTIQSLNLSWNRLRAVPNLRDLPLRYLSLDGNPL
                     ATINPGAFMGLAGLTHLSLASLQGILQLPPHGFRELPGLQVLDLSGNPKLKWAGAEVF
                     SGLGLLQELDLSGSSLVPLPETLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSP
                     KVVLHCGDTQESARGPDIL"
ORIGIN      
        1 cgccgggtcg cctaggtgag cgtgccgagg cgacagctgc cgacctgcca ggtgggtgct
       61 gtcccgaagc gcagcgagat cggcgccggt ttggaggttt ggagcccggg acttcaacct
      121 caccgcccca tcctgtgtca ccatgttgtg caccctgttc ctgctactgc tggccctggg
      181 cacagtgcag acaactcggc catgtttccc tggctgccag tgtgaggaag agacgtttgg
      241 cctctttgac agtttcagcc tgatccgagt ggactgcagc agcctgggcc cccacattgt
      301 gcctgtgccc atccctctgg acacagccca cctggacctg tcttccaacc ggctagaaac
      361 cgtgaatgag tcagtcctgg gagggccagg ctataccaca ctggctggcc tggatctcag
      421 tcacaacctc ctcaccagca tcacgcccac tgccttctcc cgccttcgct acctggagtc
      481 actggacctc agtcacaatg gcctggcagc cctgccagca gaggttttca ccagctcccc
      541 cttgagtgat atcaacctga gccataatcg acttagagag gtctcaatat ctgccttcac
      601 cacccacagc caggggcggg cactgcacgt ggacctatcc cacaatctta tccaccgcct
      661 gctcccctat ccagccaggg ccagcctgtc cgcacctacc attcagagcc tgaacctgtc
      721 ctggaaccgg ctccgagccg tgcccaatct ccgagaccta cccctgcgtt acctgagcct
      781 ggatgggaac cctctggcta ccatcaaccc aggcgccttc atggggctgg cgggcctcac
      841 ccacctttca ctggcaagcc tacagggtat cctccagcta ccaccccatg gcttccgaga
      901 gctcccaggc cttcaggtcc tggacttgtc tggtaacccc aagctcaagt gggcaggagc
      961 cgaggtattt tcaggcctgg gtttgctgca agaactagac ctgtctggct ccagcctggt
     1021 gcccctgcct gagacgctgc tacatcacct ccctgcttta cagagtgtca gtgtaggcca
     1081 agatgtgcag tgccggcgtc tggtacggga gggtgcctac caccgccaac ccggttccag
     1141 ccctaaggta gtcctgcact gtggagacac ccaggaatct gccaggggcc cagacatctt
     1201 gtgacgaatg gtgtggcctg aggccacttg acagactgct gtcctgggct tgctcaggtc
     1261 cccactaata ttttaatgtg ccaatgcctg tggagtgtct acaggtctaa gtggcagcca
     1321 tctcagtgaa gatgtgggcc aaggatagtt ttcataccta gggcctatac ccaataacag
     1381 aatctgcccg cttgtctcag cggctcactt ccctgagcta tgagcaaagg gacttcgatg
     1441 ccaaaccaga ctttggtccc ttcctgagcc ctgccccagt tatctctaag tgccttccct
     1501 tttgcccaga ctgacctgac ccatgatgta aggaccgtag gggagcacca ccactgcaaa
     1561 ggctgactaa ggtctgttgg gctaaaggtc tctttgagcc cagtgtctat agtcacacct
     1621 tctttgctct gggcccagga ggcctacttc ttctttttct cgtggaatcc tggaatctta
     1681 aagataaaag aacctagaaa gaaaatcaaa cccattttcc ttgtggggca gatggtaata
     1741 tgggactgag aacagcaaac ctggggtctt ggagaggagc ccagcctcct gttgaggttg
     1801 agagctctgt caccctccct tcccctgctg ggtaccctgt tactattgtc tgtactgttt
     1861 tccattgtgc ccagcagtag caacaacaag gtcacagagg tgggaactct aggtccagtg
     1921 gccagcttat ggtgtgagcg tagtcagtcc ccctttcaga gcctcaggaa gcttggagca
     1981 cctcagtctc aagcctgtca gttccttact ctgggatgac ccccaacatc aagactggaa
     2041 acatacacat tctccccagg gacacagtct taagatgctt ttctaagaag tcaatgcagt
     2101 tgtgaccatg tggccatgag ctccaaggga ctcgtgggct tggtgtccat gggccggagc
     2161 ccaaagaccc gcttccccct ccaagagtat ggcagaaagg gtctcccatg cacattgtgc
     2221 tccccgccag ggccacatct cagctctcca gctctggatt ctttcttctg ttttcatttt
     2281 atgagtcgtt gtctttttta aaatgaactg tttaaccccc acctctgctg gccaggggat
     2341 ggaaaggaag ataaatgtat ttgttcgctt ttgtaagact ctgttctggg tgtgggctgg
     2401 ggatcttggg aagaagccag ttggctatca gtaaccatgt gggcattggg gctggtccct
     2461 gggatgctca aaagtcagtg agagctctgt cttctcacac tagcccagcc cagcccaccc
     2521 cgtgactggt ccttggttta ataaacacat gtaagagctc tttccatcac tgga
//
