LOCUS       XM_039085230            5609 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus TSC complex subunit 2 (Tsc2),
            transcript variant X6, mRNA.
ACCESSION   XM_039085230
VERSION     XM_039085230.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051345.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039085230.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5609
                     /gene="Tsc2"
                     /gene_synonym="Rc"
                     /note="TSC complex subunit 2; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 27
                     ESTs, 1 long SRA read, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 22 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:24855"
                     /db_xref="RGD:3908"
     CDS             156..5468
                     /gene="Tsc2"
                     /gene_synonym="Rc"
                     /codon_start=1
                     /product="tuberin isoform X6"
                     /protein_id="XP_038941158.1"
                     /db_xref="GeneID:24855"
                     /db_xref="RGD:3908"
                     /translation="MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEI
                     LRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVL
                     ALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEEL
                     AEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIAPMVHMICLLCIRTVSSVDIEVS
                     LQVLDAVVCYNCLPAESLPLFIITLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNM
                     CRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVV
                     SYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVE
                     ELCDQNEFHGSQERYYELVESYADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLME
                     RFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLA
                     TQLLVDLAEGCHTHHFNSLLDIIEKTKLYTLPASHATRVYETLISHIQLHYKHGYSLP
                     IASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCAELDRASEKKASGPL
                     SPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKV
                     LIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNY
                     LDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATA
                     SMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAM
                     WFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAP
                     KQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQA
                     DDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTV
                     TTSVGTGTRSLLGLDSGDLQGGSASSSDPGTHVRQTKEAPAKLESQAGQQVSRGARDR
                     VRSMSGGHGLRVGVLDTSAPYTPGGPASLGAQAAPAARPEKPCAGAQLPAAEKANLAA
                     YVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKE
                     HRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAVVLE
                     EGSPGEAHVPVEPPELEDFEAALGTDRHCQRPDAYSRSSSASSQEEKSHLEELAAGGI
                     PIERAISSEGARPTVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKTDIGRLSPEAK
                     VRSQSGILDGEAATWSAPGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRR
                     GKRVERDNFKSRTAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSV
                     QLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQP
                     DKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVS
                     IIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSD
                     RNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNP
                     SLPLMHPPAHTKVPAQAPTEATPTYETGQRKRLISSVDDFTEFV"
ORIGIN      
        1 agctcaactc agatagtctg caaagcctcg cccccggaag tacaggttgc gcttccggca
       61 gctgctctgg gccgggggtg tgcgcccttt cctgggtgca ggaggccgtg cgcgcgaaag
      121 gcgcgcgcgg gttctctacc accgccctgc ccaccatggc caaaccaact agcaaagatt
      181 caggtttgaa ggagaagttc aagatactat taggactggg aacgtcaagg ccaaatccta
      241 ggtgtgcaga aggcaaacag acggagttta tcatcacagc ggaaatcttg agagaactga
      301 gtggtgaatg cggcctcaac aatcgaatcc ggatgatagg gcagatctgt gatgtggcaa
      361 aaactaagaa acttgaagag catgcagtgg aggcactttg gaaggctgtc tcagacttgc
      421 tacagccaga gcggccaccg gaggcccgac atgcagttct ggccttgttg aaggccattg
      481 tgcagggaca gggtgatcgt ttgggggttc tccgagccct cttcttcaaa gtgatcaagg
      541 actatccttc caacgaagac ctccatgaaa ggctagaggt ttttaaggcc ctcacagaca
      601 atggaaggca tattacctac ttggaagaag aactggcaga gtttgtcctg cagtggatgg
      661 atgttggctt gtcctcagaa ttccttctgg tactcgtgaa cctggtcaag ttcaacagct
      721 gctaccttga cgaatacatt gcgccaatgg ttcacatgat ttgtctgtta tgtatccgga
      781 cagtgtcctc tgtggacatc gaggtgtcct tgcaagtgct ggatgctgtg gtctgctaca
      841 actgcctgcc tgctgagagc ctgcctctgt tcattatcac cctgtgccgc accgtcaacg
      901 tcaaggagct gtgtgagccg tgctggaagc tgatgcgaaa ccttctaggt acccatctag
      961 gccacagtgc catttacaac atgtgccgta tcatggagaa cagatcctac atggaagacg
     1021 cccccttgtt gaggggagcc gtgttctttg ttgggatggc gctctgggga gctcaccgac
     1081 tctattcgct caagaactcc cccacatctg tgctgccgtc tttttatgag gccatgacct
     1141 gtcccaatga ggtggtgtca tatgagattg ttctgtccat aacaagactc atcaagaagt
     1201 acaggaagga gctccaggct gtgacatggg acattctgct ggacatcatt gaacgactgc
     1261 tccagcaact ccagaacctg gacagcccgg aactcaggac cattgtccat gacctgctga
     1321 ccactgtgga ggagctgtgt gaccagaacg agttccacgg atcgcaagaa agatactatg
     1381 aactggtgga gagctatgca gaccagaggc ctgaatcctc tctcttaaac ctgataacct
     1441 acagagccca gtctatccac ccagccaagg atggctggat ccagaacctg cagttgttga
     1501 tggagaggtt cttcaggaac gagtgccgca gtgccgtgcg catcaaggtg ctggacgtgc
     1561 tgtccttcgt gctcctcatc aacaggcagt tctacgagga ggagctgatt aactcggtgg
     1621 tcatctcaca gctctcccac atccccgagg ataaagacca tcaggttcga aagctggcta
     1681 cccagctgct ggtggacctg gcagagggct gccacaccca ccacttcaac agtctgttgg
     1741 acatcattga aaagaccaag ctgtatacct tgcccgccag ccatgccaca cgagtgtatg
     1801 agacactcat tagccacatc cagctccatt acaagcacgg ctactccttg cccattgcta
     1861 gcagcatccg gctgcaggcc tttgacttcc tgctgctact gcgggctgac tcgctgcatc
     1921 gactgggcct gcccaacaag gatggggtcg tgcgattcag cccttactgc ctctgtgact
     1981 gcgcggaact ggacagggcc tcagagaaga aagccagcgg acccctttca ccgccaactg
     2041 ggccccccag ccctgtgcca acgggccctg ctgtgaggct cggccacctg ccctactccc
     2101 tgcttttccg tgtcctgctg cagtgtttga agcaggagac cgactggaag gtgctgaagc
     2161 tggtgctcag caaactccct gagtcgctgc gctacaaagt cctcattttc acctccccct
     2221 gcagtgttga ccagctgtct tctgctctct gctccatgct ttcagccccg aagacccttg
     2281 agcggctccg aggcacccca gaaggcttct ccagaactga cctgcacttg gctgtggtcc
     2341 ctgtactgac agcgttaata tcttaccaca actatttgga taagactaga cagcgtgaga
     2401 tggtttactg cctagaacaa ggccttatct accgctgtgc cagccagtgt gtggtagcct
     2461 tggccatctg cagtgtggag atgcccgaca tcatcatcaa ggcgctccct gtcctggtgg
     2521 tgaagctgac acacatctct gctacagcta gcatggccat ccccctcctg gagttcctgt
     2581 ctaccttggc caggctgcct cacctctaca ggaacttcgc cgcagagcag tatgcaagtg
     2641 tgtttgccat ctccttgccc tacaccaacc cctccaagtt caaccagtac attgtgtgtc
     2701 tggcccatca cgtcatagcc atgtggttca ttaggtgccg actgcccttt cggaaggatt
     2761 ttgtccctta tatcactaag ggtctgcgtt ccaatgtcct cctgtccttt gatgataccc
     2821 ctgagaagga cagtttcaga gcacggagca ccagtcttaa tgagagaccc aaaagtttga
     2881 ggatagccag agcccccaaa caaggcctga ataactctcc acctgtgaaa gaattcaaag
     2941 agagctgtgc agccgaggcc ttccggtgcc gcagcatcag tgtatctgaa catgtggtcc
     3001 gcagcagaat acagacatct cttaccagtg ccagcttggg gtctgcggat gagaactcta
     3061 tggcccaggc tgatgacaac ctgaagaatc tccacttgga actcacagaa acatgtctgg
     3121 acatgatggc ccgatatgtg ttctccaact tcactgcggt tcccaagagg tcccctgtgg
     3181 gagaattcct cctggcaggg ggcaggacca aaacgtggct ggttggaaat aagctggtca
     3241 ctgtgacaac gagtgtggga actggtacgc ggtcgttgct gggcctggac tccggggatc
     3301 tgcagggtgg ctcagcatca agctctgatc ctggcacaca tgtgaggcag acaaaggaag
     3361 caccggccaa actggagtcc caggctgggc agcaggtgtc ccgtggggcc cgggaccgag
     3421 tccgctccat gtcagggggc cacggccttc gagttggtgt cctggacact tcagctccct
     3481 acaccccagg aggccctgct tctctgggag cacaggcagc accagcagca aggcctgaga
     3541 agccctgtgc aggagcccaa cttccggcag cggagaaggc gaacctggca gcctatgtgc
     3601 ctttgctgac ccagggctgg gcagagatcc tggtccgcag gcccacagga aacaccagct
     3661 ggctgatgag cttggagaac ccgctcagcc ccttttcctc agacatcaac aacatgcccc
     3721 tgcaagagct gtctaatgcc cttatggctg ctgagcgctt caaggagcac cgggacacgg
     3781 ccctgtacaa gtcattgtca gtgccagcgg ctggcacagc caagcctcct actctcccac
     3841 gttctaacac agtggcttct ttctcctccc tgtaccagcc cagctgccaa ggacagctgc
     3901 acaggagcgt ttcctgggca gactcggccg tggttcttga ggagggaagt ccaggagagg
     3961 ctcatgtgcc agtggagccc cccgaattgg aggactttga ggcagcatta ggtacagaca
     4021 ggcactgcca gcggcctgac gcctacagca ggtcttcctc agcatctagc caggaagaaa
     4081 agtcccactt ggaggagctg gctgcagggg gtatccccat tgagcgggcc atctcctctg
     4141 agggggctcg gcctacagtg gacctgtcct tccaaccctc acagcctttg agcaagtcta
     4201 gctcttctcc tgagttacag accttacagg acatccttgg agacctaggg gacaagactg
     4261 acattggacg gctgagtcct gaggctaagg tccgatccca gtcggggatc ctggatgggg
     4321 aagcagctac ctggtcagct ccgggtgaag agagccgtat cacagtccca cctgaaggtc
     4381 ccctgccttc cagttctccc cgctccccta gtggcctccg gccccgaggc tataccatct
     4441 ctgattcagc tccatcacga aggggaaaga gggtagaaag ggacaacttc aagagcagaa
     4501 ctgcggcctc cagtgctgaa aaggtgccag gcatcaaccc tagctttgtg ttcctacagc
     4561 tctaccattc accctttttt ggtgatgagt ccaataagcc catcctgttg cccaacgagt
     4621 cctttgaacg gtcagtgcag ctccttgacc agatcccatc ctatgacact cacaagattg
     4681 ctgtcctgta tgtgggagaa ggccagagca gcagtgagct ggccatccta tcaaatgagc
     4741 atggctctta taggtacaca gagtttctga caggcctggg tcggcttatt gagctgaagg
     4801 actgccaacc agacaaggtg tacttaggtg gactggatgt gtgtggcgag gatggacagt
     4861 tcacgtactg ctggcatgat gacatcatgc aagctgtttt ccacattgct accctgatgc
     4921 ccaccaagga tgtggacaag caccgttgtg acaagaaacg gcacctgggc aatgactttg
     4981 tttctatcat ctacaatgat tctggtgagg acttcaagct gggcaccatt aagggccagt
     5041 tcaacttcgt ccatgtgatc atcacaccgc tggactataa atgcaacctg ttgaccctgc
     5101 agtgcaggaa agacatggag ggccttgtgg acaccagtgt ggccaagatt gtgtctgacc
     5161 gcaacctgtc ctttgtggcc cgacagatgg ccctgcatgc aaatatggcc tcacaggtac
     5221 accacagccg atccaacccc acggacatct atccctccaa gtggatcgca agactccgcc
     5281 acattaagcg tctccgtcag cggatccgtg aagaagtgca ctattcaaac ccaagcttgc
     5341 ctctgatgca ccctccagcc cataccaaag tcccagctca agcccctacg gaggctacac
     5401 ccacctacga gacaggccag cggaaacgcc tcatttcctc tgtggatgac ttcacagagt
     5461 ttgtgtgagg ccaccagagc cacaactgga gtcccgtggc catgcacctg gcccttccct
     5521 gaatgacacc tcctgactgt actcttacat gaaataaaac tgcccgtgcc catctggccc
     5581 ttggagttca gagagcagca cagacagca
//
