LOCUS       NM_021854               7817 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus TSC complex subunit 1 (Tsc1), mRNA.
ACCESSION   NM_021854 XM_006233843
VERSION     NM_021854.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Adriaanse,B.A., Brady,S., Wang,M., Beard,D.J., Spencer,J.I.,
            Pansieri,J., Sutherland,B.A., Esiri,M.M., Buchan,A.M., Cader,Z.,
            Zhang,B. and DeLuca,G.C.
  TITLE     Tuberous sclerosis complex-1 (TSC1) contributes to selective
            neuronal vulnerability in Alzheimer's disease
  JOURNAL   Neuropathol Appl Neurobiol 49 (3), e12904 (2023)
   PUBMED   37020385
  REMARK    GeneRIF: Tuberous sclerosis complex-1 (TSC1) contributes to
            selective neuronal vulnerability in Alzheimer's disease.
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Cao,H., Luo,J., Zhang,Y., Mao,X., Wen,P., Ding,H., Xu,J., Sun,Q.,
            He,W., Dai,C., Zen,K., Zhou,Y., Yang,J. and Jiang,L.
  TITLE     Tuberous sclerosis 1 (Tsc1) mediated mTORC1 activation promotes
            glycolysis in tubular epithelial cells in kidney fibrosis
  JOURNAL   Kidney Int 98 (3), 686-698 (2020)
   PUBMED   32739207
  REMARK    GeneRIF: Tuberous sclerosis 1 (Tsc1) mediated mTORC1 activation
            promotes glycolysis in tubular epithelial cells in kidney fibrosis.
REFERENCE   3  (bases 1 to 7817)
  AUTHORS   He,Y., Ao,N., Yang,J., Wang,X., Jin,S. and Du,J.
  TITLE     The preventive effect of liraglutide on the lipotoxic liver injury
            via increasing autophagy
  JOURNAL   Ann Hepatol 19 (1), 44-52 (2020)
   PUBMED   31787541
REFERENCE   4  (bases 1 to 7817)
  AUTHORS   Woodford,M.R., Sager,R.A., Marris,E., Dunn,D.M., Blanden,A.R.,
            Murphy,R.L., Rensing,N., Shapiro,O., Panaretou,B., Prodromou,C.,
            Loh,S.N., Gutmann,D.H., Bourboulia,D., Bratslavsky,G., Wong,M. and
            Mollapour,M.
  TITLE     Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates
            folding of kinase and non-kinase clients
  JOURNAL   EMBO J 36 (24), 3650-3665 (2017)
   PUBMED   29127155
REFERENCE   5  (bases 1 to 7817)
  AUTHORS   Lim,J.S., Gopalappa,R., Kim,S.H., Ramakrishna,S., Lee,M., Kim,W.I.,
            Kim,J., Park,S.M., Lee,J., Oh,J.H., Kim,H.D., Park,C.H., Lee,J.S.,
            Kim,S., Kim,D.S., Han,J.M., Kang,H.C., Kim,H.H. and Lee,J.H.
  TITLE     Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia
  JOURNAL   Am J Hum Genet 100 (3), 454-472 (2017)
   PUBMED   28215400
REFERENCE   6  (bases 1 to 7817)
  AUTHORS   Nellist,M., van Slegtenhorst,M.A., Goedbloed,M., van den
            Ouweland,A.M., Halley,D.J. and van der Sluijs,P.
  TITLE     Characterization of the cytosolic tuberin-hamartin complex. Tuberin
            is a cytosolic chaperone for hamartin
  JOURNAL   J Biol Chem 274 (50), 35647-35652 (1999)
   PUBMED   10585443
REFERENCE   7  (bases 1 to 7817)
  AUTHORS   Satake,N., Kobayashi,T., Kobayashi,E., Izumi,K. and Hino,O.
  TITLE     Isolation and characterization of a rat homologue of the human
            tuberous sclerosis 1 gene (Tsc1) and analysis of its mutations in
            rat renal carcinomas
  JOURNAL   Cancer Res 59 (4), 849-855 (1999)
   PUBMED   10029074
REFERENCE   8  (bases 1 to 7817)
  AUTHORS   Plank,T.L., Yeung,R.S. and Henske,E.P.
  TITLE     Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene,
            interacts with tuberin and appears to be localized to cytoplasmic
            vesicles
  JOURNAL   Cancer Res 58 (21), 4766-4770 (1998)
   PUBMED   9809973
REFERENCE   9  (bases 1 to 7817)
  AUTHORS   van Slegtenhorst,M., Nellist,M., Nagelkerken,B., Cheadle,J.,
            Snell,R., van den Ouweland,A., Reuser,A., Sampson,J., Halley,D. and
            van der Sluijs,P.
  TITLE     Interaction between hamartin and tuberin, the TSC1 and TSC2 gene
            products
  JOURNAL   Hum Mol Genet 7 (6), 1053-1057 (1998)
   PUBMED   9580671
REFERENCE   10 (bases 1 to 7817)
  AUTHORS   van Slegtenhorst,M., de Hoogt,R., Hermans,C., Nellist,M.,
            Janssen,B., Verhoef,S., Lindhout,D., van den Ouweland,A.,
            Halley,D., Young,J., Burley,M., Jeremiah,S., Woodward,K.,
            Nahmias,J., Fox,M., Ekong,R., Osborne,J., Wolfe,J., Povey,S.,
            Snell,R.G., Cheadle,J.P., Jones,A.C., Tachataki,M., Ravine,D.,
            Sampson,J.R., Reeve,M.P., Richardson,P., Wilmer,F., Munro,C.,
            Hawkins,T.L., Sepp,T., Ali,J.B., Ward,S., Green,A.J., Yates,J.R.,
            Kwiatkowska,J., Henske,E.P., Short,M.P., Haines,J.H., Jozwiak,S.
            and Kwiatkowski,D.J.
  TITLE     Identification of the tuberous sclerosis gene TSC1 on chromosome
            9q34
  JOURNAL   Science 277 (5327), 805-808 (1997)
   PUBMED   9242607
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000115.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_021854.3.
            
            On or before Oct 27, 2022 this sequence version replaced
            XM_006233843.3, NM_021854.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB011821.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMD00132262, SAMD00132263 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                JACYVU010000115.1  5426546-5426610
            66-128              JACYVU010000115.1  5436552-5436614
            129-314             JACYVU010000115.1  5443581-5443766
            315-418             JACYVU010000115.1  5445322-5445425
            419-571             JACYVU010000115.1  5446083-5446235
            572-716             JACYVU010000115.1  5447096-5447240
            717-871             JACYVU010000115.1  5448469-5448623
            872-945             JACYVU010000115.1  5448978-5449051
            946-1121            JACYVU010000115.1  5456180-5456355
            1122-1237           JACYVU010000115.1  5457086-5457201
            1238-1352           JACYVU010000115.1  5457540-5457654
            1353-1471           JACYVU010000115.1  5457901-5458019
            1472-1541           JACYVU010000115.1  5460462-5460531
            1542-1646           JACYVU010000115.1  5460952-5461056
            1647-2205           JACYVU010000115.1  5461316-5461874
            2206-2249           JACYVU010000115.1  5463313-5463356
            2250-2416           JACYVU010000115.1  5464070-5464236
            2417-2599           JACYVU010000115.1  5465326-5465508
            2600-2710           JACYVU010000115.1  5466483-5466593
            2711-2833           JACYVU010000115.1  5467184-5467306
            2834-3021           JACYVU010000115.1  5469969-5470156
            3022-3183           JACYVU010000115.1  5470227-5470388
            3184-7817           JACYVU010000115.1  5470776-5475409
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..7817
                     /gene="Tsc1"
                     /note="TSC complex subunit 1"
                     /db_xref="GeneID:60445"
                     /db_xref="RGD:620124"
     exon            1..65
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            66..128
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            129..314
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..3700
                     /gene="Tsc1"
                     /note="tuberous sclerosis 1 protein homolog"
                     /codon_start=1
                     /product="hamartin"
                     /protein_id="NP_068626.1"
                     /db_xref="GeneID:60445"
                     /db_xref="RGD:620124"
                     /translation="MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNT
                     LVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPS
                     WKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGR
                     LSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEE
                     VVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDA
                     VSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLS
                     TRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPA
                     TSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGS
                     KGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFY
                     RDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASP
                     AGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKT
                     EELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGS
                     PPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQL
                     KLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQT
                     KLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLG
                     EVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELES
                     LLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKD
                     GRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASC
                     GGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMK
                     TRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDS
                     MGQLHIMDYNETHHEHS"
     exon            315..418
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            419..571
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            572..716
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            717..871
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            872..945
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1121
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1237
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1352
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1471
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1541
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1646
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..2205
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2249
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2416
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2599
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2710
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2833
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..3021
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3022..3183
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..7817
                     /gene="Tsc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agatggcggc gccctgtagg ctggagggac tgtgaggtaa acagctgagg gggaggagac
       61 ggtggtgacc atggaagaca caaggctggc agcactggaa actgatgtac cgtctgtccc
      121 cagaacagtc tggtagtggc cctaatgaag agctgtggaa gcctttagca catgtcctgg
      181 agcccacaca gcataattga gtgagagaat ggcccagcta gccaacattg gggagctact
      241 ctccatgctg gactcttcca cactgggtgt gcgtgatgac gtgacaacca tcttcaagga
      301 gtccctcaat tctgaacgtg ggcctatgct tgtaaacacg ttggtggatt attacctgga
      361 aaccaattct cagccggtct tgcacatcct gaccaccctg caagagccac atgataagca
      421 cctcttggac aaaatgaatg agtatgtagg caaagctgcc acccgcttat ccatcctctc
      481 gctgctgggg catgttgtga ggctgcagcc atcttggaag cataagctct ctcaagcacc
      541 tcttctgcct tctttactga aatgtctcaa gatggacact gatgttgtgg ttctcacaac
      601 tggtgtcttg gtgttgatca ccatgctacc gatgatcccg cagtcaggga agcagcacct
      661 tctcgacttc tttgacatct ttggccgtct ttcatcatgg tgcctgaaga aaccaggcca
      721 tgtgacagaa gtctacctgg tccacctcca tgccagtgtt tatgccctct tccatcgcct
      781 ttatggaatg tacccttgta acttcgtctc cttcctgcgc tctcactaca gcatgaagga
      841 aaacgtggag acttttgaag aagtggtcaa gccaatgatg gagcatgtgc gaattcaccc
      901 ggaattagtg actggatcca aggaccatga actggaccct cgaaggtgga agacattaga
      961 aactcatgat gttgtaatag agtgtgccaa aatctctctg gaccctaccg aagcctcata
     1021 tgaagatggc gatgctgtgt ctcaccagct ctctgcctgc ttccctcacc gttcagctga
     1081 tgtcaccacc agctcttacg tggacacaca gaatagctat gggggcgcta cttccacccc
     1141 ttcctccacc tctcggctga tgttgttcag tacccccggg cagctacctc agagtttgag
     1201 ttcactatca acacggccgt tacctgagcc gctgcaagct agtctttgga gcccatctgc
     1261 agtctgtggt atgaccactc ctcctacgtc tcctggaaat gtcccggctg atttgtcaca
     1321 tccatacagt aaagcctttg gtactaccac tggtggaaaa ggaactcctt caggaacccc
     1381 agcgacctct cctcccccag ccccgccttg tccccaagac gactgtgcgc acggtccagc
     1441 ctcccaggcc tcagccacac cccccaggaa ggaagaaaga gcagattctt caaggcctta
     1501 cctacccaga cagcaggatg ttccgagtga ccgcggatta gaggatctac ctggaagcaa
     1561 aggttccgtc actctgagga atctaccaga tttcctaggt gacctggctt cagaggaaga
     1621 cagtatagag aaagataagg aagaagctgc gatatctaaa gagctttctg agatcaccac
     1681 tgcagaggcg gatcctgtag ctcctcgagg gggctttgac tctcccttct accgagacag
     1741 tctctctggt tctcagcgga agactcactc agcagcctct gggactcagg gcttcagcgt
     1801 gaaccctgag cctttgcact cctccctgga caaacatggg cctgacacac caaagcaagc
     1861 ctttactccc atagacccac cctctggcag tgctgatgcc agtcccgctg gggacaggga
     1921 tcgccagact tctctggaga ccagtatcct cactcccagc ccttgcaaaa ttccacctca
     1981 gaggggagtg agctttggaa gtgggcagct tcccccatat gatcatctct ttgaggtggc
     2041 cttgccaaag actgcctgtc actttgtcag caaaaagact gaagagctgt tgaagaaagc
     2101 gaaaggaaac cctgaggaag actgtgtgcc ctctacctcc ccaatggaag tactggacag
     2161 actgctagag cagggagcag gggcacacag caaggaactg agcaggttgt ccttgcccag
     2221 caagtctgtt gactggaccc actttggagg ctctcccccc tcagatgaga tccggaccct
     2281 ccgagaccag ttgctcttac tgcacaatca gctgctgtac gaacgcttta agcggcagca
     2341 gcatgcactg aggaacagaa ggctgctgcg caaggtgatc cgagctgcgg ccctggagga
     2401 gcacaacgca gcgatgaaag atcagttgaa gttacaagag aaggacatcc agatgtggaa
     2461 ggtgagtctg cagaaagaac aagcccgata cagtcagctt cagcaacaac gtgacaccat
     2521 ggtgacccag ctgcatagcc aaatcagaca gctgcagcat gaccgagaag aattctacaa
     2581 ccagagtcag gagttacaga cgaagctgga ggactgcaga agcatgatcg cggagcttcg
     2641 agtggagctg aagaaggcca acagcaaggt gtgccacacc gagctgctgc tcagccaggt
     2701 ctctcagaag ctctccaata gtgagtcagt gcagcagcag atggagttct tgaataggca
     2761 gcttctggtg ctcggggagg tcaatgagct gtacctggag cagctgcaga gcaagcatcc
     2821 tgacaccacc aaggaagtag aaatgatgaa aactgcttat cggaaagaac tagagaaaaa
     2881 cagaagccac cttctccagc agaaccagag gctggatgcc tcacagaggc gagttttaga
     2941 actggaatct cttctggcca agaaagacca ccttctccta gagcagaaga agtaccttga
     3001 ggatgtcaag agccaggcga gtggacagct gctggctgca gagagcaggt atgaggctca
     3061 gagaaagatc acccgggtgt tggaactgga gatcctagac ttgtatggta gattggagaa
     3121 agatggccgc ctacagaaac tagaagagga cagagcagag gcagcagagg cagctgaaga
     3181 gaggcttgac tgttgtactg atggatgctc agattccttg ttaggacata atgaagaggc
     3241 tgctggtcac aatggtgaga ccaggacctc cagacctggt ggcacccggg ccagctgtgg
     3301 aggtagagtc actggaggca gcagcagcag cagcagtgag ctttccactc cagagaaacc
     3361 cccgaaccag aggttcagca gccggtggga gcccaccatg ggtgagccct ccagcagcat
     3421 ccccaccact gttggctcac ttcccagttc caaaagcttc ttgggcatga agacccggga
     3481 gctgttccgt aataagagtg agagccagtg tgatgaggat ggcatgacca tgagtagctt
     3541 ttctgagacc ctgaagacag aactgggcaa agactcggcg ggcatggaaa acaagactcc
     3601 cccaagtcta gacgccccac acccatcttc cccaagctca gacagtatgg ggcagctcca
     3661 tatcatggac tacaatgaga ctcatcacga acacagctga agagggtctg tcagtgttaa
     3721 tttgcgtctt tgtcggcaca gaacagaagg tgtgaatgca cagtgtaggc ttctctgttt
     3781 ccagggtctg agcagctgct tgtgtggtgg gaatgggaca gaggtccttt tgacagaggg
     3841 tagaatgtgg gatgatggtt tttgggtctg gcattgagac gcttcccttc acctcacccc
     3901 agcctctttc ctctacccag cagtatgtac taattgaggg cctgtgcttc attttcttcc
     3961 accaaatggt tatgtcattt gaacctgtgc aatatgaggc cagatttcat ctttgggtcc
     4021 aacataccac tctgctctcc tgaggttcag acagctgggt ggctcttggg tagaccaggc
     4081 agttagtggc actgcaggta agtccagttt tgtccctgcc ggggggacat aacgtacaaa
     4141 gttatcttag caggagcatt cccactgctt tttggttctg aagtttagca tctaagccac
     4201 agttctagag aaaatggctc gctcacctct gccttttctt ggcagctctg acatgcttcc
     4261 tagaaggttc tgtgagaata ccagctgctt tgataatgca gttggcactg tggaggccgc
     4321 actgccttac ctctctagaa agcccttacc cctctgcccc actacctctt attgatttgg
     4381 tatttgcttg aatgctggca ccgtgctgcc tgttgggtgg gtgtataggt ggtcgacctg
     4441 cagtctgcag acagcgggga gtggtcacta ggaggggcaa ctaggattcc caggctcgtg
     4501 tgcctctggc tggccatggc tccacccttc agcctgtttg cctcccctcg ggtccagctg
     4561 ctttgtgatt ttgcagcaga cagactggga agaagtcagc tccttggggc ttatgtgatg
     4621 atggctctcg ggctgaggga tcgcctcaca gtgctaggtt ctcacagctt ttacaccaat
     4681 tccaactcac aggaaagact ctctggagct tcatatcaaa cacttctgtt aggaacagca
     4741 gagcaattca gagtctgata aaggctggtg gcctatatca ctgcctgccc tcatgctcag
     4801 gagaggtgac atcggtcaca gtcagagcac aatggtggca gagcccaaac tcaaccagtg
     4861 ctcctgaaat aaatgctgga agtgtaagag ttgttgcctg atattctggc ccagtcaagg
     4921 agggacttgg gaaggagcca tgagcttgga tttcttgttt ctattctgtc tgatgtacaa
     4981 agaaaagggt ttagttacga caaagctaaa acaaaactaa actaatggta cgactcttac
     5041 tgcatgtgaa agtttccggc ctgagggtaa aagcctttct caagtgtggc agtaaatgtc
     5101 ctagcatccc taagagacaa gacttcatgt caccatggca gcgctttctg gagacctgtc
     5161 agggccatgt ctctgccata gctgacacag ccagctcaca ggaaggatgc cactgaccac
     5221 tctcagacca gcagagggca gctatcgcct ctgcacactt ggagtgtggg tgtcagctgg
     5281 cgtgcttctc atgtagctct gcccaaagca aagctaaagc ccgatatcct ctcccccagt
     5341 ccattgtaga aatggcagat taacagatct cgcaggttaa tggtaaacag tttccaagag
     5401 ttaccacctc caccaccaag atctgccaga gaacaaaata ttgagaaaga aaacagactg
     5461 tacaggtgat ggtgtttagg gagaagaggc agcagcacgg tgaggtgagc taatgtgtag
     5521 gccgccatct ccatagattt cacttgggcc ctccgcactt tctccagcct gttcccacgc
     5581 ccagtggcag agcctgaaca tcctgtgtgg cttgagttct agaaggctgt taagactttt
     5641 ttcatccaaa agggttggag gactgtgacc cagagtcatc tttaaggagc taaagattgg
     5701 ctccttcaaa ggactccagg atcagtgtgt ggttgggtgg cttccatttt agagagcagt
     5761 gacagatgat ctgggagtca gtgggtcacc tggtattccc agaggcatga gcaaagcttc
     5821 tgagaagcag ctgagttctg ggccaaggca ttagagcagg aggtgtcatg ggcagccttg
     5881 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgag tgtgggggtc gaatggcctt
     5941 catcctctaa tcacttagaa catgtgctgt gcttgctgat gggccccagg gagactggag
     6001 gctgtgtctc atgtagtcca gaatctgtta gaagcagccc tacagtgcca cggtagtgga
     6061 agaggtcagt gcccgggaga ggagctctgt acatatctag agaacatccc tccctgaagc
     6121 ctaagcacgg ggtttgggaa caagagcagt atggcagtga gaatgaggag acagggggaa
     6181 tctctggagt cacagcatag gtaccagggc tgaatgctga gctcagtagc ttttctgacc
     6241 cttcggagaa aaggcctgct cttctggact aagttgcaga agggtccagc tgtgagacat
     6301 ggttagcagg gagtggcttc tgagaaggca aactcatgac ccaggagaag tctggtgact
     6361 actggtggtt taaaaagcag acaaaaatct ccccacccca tgctgctgca gctaggaacc
     6421 aagcatctcc caggacggtg gtggcactat aggtgacaag ctgtagactc agggccactt
     6481 gcagaatctc tgagctctag tgctcaaagg agaatgagaa ttagtagtca aggataaatg
     6541 gacctgagac ctggccacag agctcaggct gggagtaagc ttagaaagca aggaaggcgt
     6601 gacaaccgaa gtgcagtgtg accagccagc aggaaactac cacctaaaga actacgccag
     6661 aaagaactaa ggagcaaaca gccataccat ataggaaggt aggagcagtg ggggtgggag
     6721 accctggaca agctggtagc cgtgtgggca ttgcacaggc tcagtttggg ctgcagcatg
     6781 tgcagtctgc ctcagccaga ggccagtggc cctgcagact gggaccgaat ctgctgcacc
     6841 tttgctccag gcccatactg taaatcactg ctgtgtgcac tggaaagcaa acctcagtgt
     6901 gaggtgtctg tgtccctgtg aacatccaga ggtctggttt gtacctccgg cctccagttg
     6961 gctgcagagt cgctgattgt cttgggatta gcctgtgtag atgtagaaag ggggcggaag
     7021 acaggtccct gggttgctgc gtccagtctt gttctcattc agctttccag tctgtgagtc
     7081 cccggtatgt tcaggagagc agcgcgtcca tctctggtat caccctgagt tttaaatgct
     7141 gtatagaaag gcagcaacag ctacaacaga aattgcaccc tgaccacttt tgatcttaga
     7201 atgaaattaa ttggggtctc agaagtgttc ccccttccca gggctcccgc attatccacg
     7261 tctggataag taggcagcct gagccactgg agtccgaggg cgctgggctg accctcttcc
     7321 tgcctcagcc cacacagtgt tgaagggaga agaggacacg atgctgtctg tcagctccct
     7381 ggcacatcgc aggcaggcag gcattttttg cactaggaag ttcagtgtca cccttcttga
     7441 gactggacca caaacacatt atggaggagc aggttttgcc aagacataac ttagttttat
     7501 aatcaatcat ggatgaacca tatatggcta acttggagtt taaggggggg gggggttccc
     7561 atcagtgaaa acaaatcaaa aatttaaaaa actttttagt tcaatggaag tcacttctca
     7621 aaaggtttaa actttcccac tgtgatctta aaagcatgtc aaacactctg aatcagatgc
     7681 cataagtatg ggctgcagga gaacatggta cagtctcagt gaatggaatc aaaggtttgc
     7741 tgtccttaga atgttcgaaa atgtcaaggc agttgcttgt gtttaactgt gaacaaataa
     7801 agatttattg ttttgca
//
