LOCUS       NM_001014257            1320 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus TruB pseudouridine synthase family member 2
            (Trub2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001014257 XM_345331
VERSION     NM_001014257.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Arroyo,J.D., Jourdain,A.A., Calvo,S.E., Ballarano,C.A.,
            Doench,J.G., Root,D.E. and Mootha,V.K.
  TITLE     A Genome-wide CRISPR Death Screen Identifies Genes Essential for
            Oxidative Phosphorylation
  JOURNAL   Cell Metab 24 (6), 875-885 (2016)
   PUBMED   27667664
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 1320)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB613146.1 and BC084712.1.
            
            On Oct 12, 2007 this sequence version replaced NM_001014257.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ231592.1, CO384664.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760433, SAMEA5760494
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                CB613146.1         1-25
            26-1320             BC084712.1         1-1295
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..1320
                     /gene="Trub2"
                     /note="TruB pseudouridine synthase family member 2"
                     /db_xref="GeneID:366012"
                     /db_xref="RGD:1359394"
     exon            1..148
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1011
                     /gene="Trub2"
                     /EC_number="5.4.99.25"
                     /note="probable tRNA pseudouridine synthase 2; TruB
                     pseudouridine (psi) synthase family member 2;
                     mitochondrial mRNA pseudouridine synthase Trub2; psi55
                     synthase TRUB2; tRNA pseudouridine 55 synthase TRUB2; truB
                     pseudouridine synthase homolog 2"
                     /codon_start=1
                     /product="pseudouridylate synthase TRUB2, mitochondrial"
                     /protein_id="NP_001014279.1"
                     /db_xref="GeneID:366012"
                     /db_xref="RGD:1359394"
                     /translation="MGSSGLARLQGLFAVYKPPGLKWLHVRETVELQLLKGLNAQQPS
                     APEQHVRFLLGPVEGSEEKKLTLTATSVPSLTTHRLVRGPAFRNLKIGVGHRLDVQAS
                     GVLVLAVGHGRSLLTDMYDAHLTKDYTVRGLLGKATDNFCEDGQLIEKTTYDHVTRER
                     LDRILAMIQGSHQKALVMYSNLDLKSQEAYERAVQGVIRPMNKSPMLIAGIRCLHFAP
                     PEFLLEVQCMHETQQQLRRLVHEIGLELKTTAVCMQVRRTRDGFFGLDDALLRTQWDL
                     HSIQDAIQTAAPRVARELRKNLSLMSGHQQQLPSAGQPWASRVQAPL"
     exon            149..280
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            281..355
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            356..417
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            418..499
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            500..572
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            573..709
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
     exon            710..1300
                     /gene="Trub2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcgcttct ggatgatgta gtactgcaga gttccagcca tgggttcttc tggcctagct
       61 cgactgcagg gtctcttcgc tgtctataaa cctccggggc tgaaatggct gcatgtgcgt
      121 gagaccgtgg agttgcaact gcttaaaggt ctcaatgcac agcagccttc tgctccagaa
      181 cagcatgttc gcttcttact aggccctgtg gaaggcagtg aagagaagaa gttgactctc
      241 acggccacca gtgtgccctc cctcaccacc cacagacttg ttcgtggtcc agcattcaga
      301 aacctgaaga tcggtgtggg gcatcgtttg gatgtccagg cttctggtgt gcttgttctc
      361 gctgtgggac acggacgcag tctcctcact gacatgtatg atgctcacct caccaaggat
      421 tacacggttc gtggcctcct gggcaaagct acagacaact tctgtgaaga tgggcagttg
      481 atagagaaga caacatatga ccatgtgact agagagcggc tggaccggat cctggctatg
      541 atccaaggct cccaccaaaa agccctggta atgtactcca acctggacct gaagtcccag
      601 gaggcctacg agagggctgt gcaaggcgtg atccggccca tgaacaagtc cccaatgctc
      661 atcgctggca tccgctgcct gcactttgca cctcctgagt ttcttttgga ggtgcagtgt
      721 atgcacgaga cacagcagca gctgaggagg ctggtgcatg aaatcggcct ggagctgaag
      781 accaccgctg tctgcatgca agtgcggcgc actcgggatg gcttcttcgg gctggacgat
      841 gccctcctga gaacccagtg ggacctgcac agcattcagg atgctattca gacggcagcc
      901 ccccgggtag ccagagaact gagaaagaac ttgagcctaa tgtcgggcca ccaacaacag
      961 ctccccagtg ccgggcagcc ttgggcttca agggtccaag ctccactttg aaagtgggag
     1021 ccatgcaggg tagtaaggcc aggcaggcgc ctggatgtag gggcaaacag gctttcaata
     1081 gtcagaactg gtcactctgc agagagtgag atcaaggagg gccctgagct gtcatctcag
     1141 caaaacaaaa gtacaggagg gagccttctt cctgtcacac acaaactaac cacagaggtg
     1201 agtgggtaag ctatcatcgc agctagttgg gaggcccagg ttagaatctc gacagttcaa
     1261 ggccagccta gggttactta gtgaaacctt gactcaaaaa aaaaaaaaaa aaaaaaaaaa
//
