LOCUS       NM_053705               7118 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus transient receptor potential cation channel,
            subfamily M, member 7 (Trpm7), mRNA.
ACCESSION   NM_053705 XM_001056331 XM_002726172
VERSION     NM_053705.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Kim,I.Y., Park,C.S., Seo,K.J., Lee,J.Y. and Yune,T.Y.
  TITLE     TRPM7 Mediates Neuropathic Pain by Activating mTOR Signaling in
            Astrocytes after Spinal Cord Injury in Rats
  JOURNAL   Mol Neurobiol 61 (8), 5265-5281 (2024)
   PUBMED   38180616
  REMARK    GeneRIF: TRPM7 Mediates Neuropathic Pain by Activating mTOR
            Signaling in Astrocytes after Spinal Cord Injury in Rats.
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Lin,Y., Chen,Y., Hu,W., Liu,X., Hao,W., Xing,J., Ding,J., Xu,Y.,
            Yao,F., Zhao,Y., Wang,K., Li,S., Yu,Q., Hu,W. and Zhou,R.
  TITLE     TRPM7 facilitates fibroblast-like synoviocyte proliferation,
            metastasis and inflammation through increasing IL-6 stability via
            the PKCalpha-HuR axis in rheumatoid arthritis
  JOURNAL   Int Immunopharmacol 132, 111933 (2024)
   PUBMED   38581988
  REMARK    GeneRIF: TRPM7 facilitates fibroblast-like synoviocyte
            proliferation, metastasis and inflammation through increasing IL-6
            stability via the PKCalpha-HuR axis in rheumatoid arthritis.
REFERENCE   3  (bases 1 to 7118)
  AUTHORS   Park,C.S., Lee,J.Y., Seo,K.J., Kim,I.Y., Ju,B.G. and Yune,T.Y.
  TITLE     TRPM7 Mediates BSCB Disruption After Spinal Cord Injury by
            Regulating the mTOR/JMJD3 Axis in Rats
  JOURNAL   Mol Neurobiol 61 (2), 662-677 (2024)
   PUBMED   37653221
  REMARK    GeneRIF: TRPM7 Mediates BSCB Disruption After Spinal Cord Injury by
            Regulating the mTOR/JMJD3 Axis in Rats.
REFERENCE   4  (bases 1 to 7118)
  AUTHORS   Shin,M., Matsushima,A., Kajiya,H., Okamoto,F., Ogata,K., Oka,K.,
            Ohshima,H., Bartlett,J.D. and Okabe,K.
  TITLE     Conditional knockout of transient receptor potential melastatin 7
            in the enamel epithelium: Effects on enamel formation
  JOURNAL   Eur J Oral Sci 131 (2), e12920 (2023)
   PUBMED   36794562
  REMARK    GeneRIF: Conditional knockout of transient receptor potential
            melastatin 7 in the enamel epithelium: Effects on enamel formation.
REFERENCE   5  (bases 1 to 7118)
  AUTHORS   Jimenez-Dinamarca,I., Prado,Y., Tapia,P., Gatica,S., Alt,C.,
            Lin,C.P., Reyes-Martinez,C., Feijoo,C.G., Aravena,C.,
            Gonzalez-Canacer,A., Correa,S., Varela,D., Cabello-Verrugio,C. and
            Simon,F.
  TITLE     Disseminated intravascular coagulation phenotype is regulated by
            the TRPM7 channel during sepsis
  JOURNAL   Biol Res 56 (1), 8 (2023)
   PUBMED   36869357
  REMARK    GeneRIF: Disseminated intravascular coagulation phenotype is
            regulated by the TRPM7 channel during sepsis.
            Erratum:[Biol Res. 2023 May 11;56(1):24. doi:
            10.1186/s40659-023-00433-6.. PMID: 37165417]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7118)
  AUTHORS   Hermosura,M.C., Nayakanti,H., Dorovkov,M.V., Calderon,F.R.,
            Ryazanov,A.G., Haymer,D.S. and Garruto,R.M.
  TITLE     A TRPM7 variant shows altered sensitivity to magnesium that may
            contribute to the pathogenesis of two Guamanian neurodegenerative
            disorders
  JOURNAL   Proc Natl Acad Sci U S A 102 (32), 11510-11515 (2005)
   PUBMED   16051700
REFERENCE   7  (bases 1 to 7118)
  AUTHORS   He,Y., Yao,G., Savoia,C. and Touyz,R.M.
  TITLE     Transient receptor potential melastatin 7 ion channels regulate
            magnesium homeostasis in vascular smooth muscle cells: role of
            angiotensin II
  JOURNAL   Circ Res 96 (2), 207-215 (2005)
   PUBMED   15591230
  REMARK    GeneRIF: TRPM7 is a functionally important regulator of Mg2+
            homeostasis and growth in vascular smooth muscle cells.
REFERENCE   8  (bases 1 to 7118)
  AUTHORS   Jiang,X., Newell,E.W. and Schlichter,L.C.
  TITLE     Regulation of a TRPM7-like current in rat brain microglia
  JOURNAL   J Biol Chem 278 (44), 42867-42876 (2003)
   PUBMED   12904301
  REMARK    GeneRIF: TRPM7 currents were identified in the rat brain microglia
REFERENCE   9  (bases 1 to 7118)
  AUTHORS   Runnels,L.W., Yue,L. and Clapham,D.E.
  TITLE     The TRPM7 channel is inactivated by PIP(2) hydrolysis
  JOURNAL   Nat Cell Biol 4 (5), 329-336 (2002)
   PUBMED   11941371
  REMARK    GeneRIF: Data show that receptor-mediated activation of
            phospholipase C results in the hydrolysis of localized PIP(2),
            leading to inactivation of the TRPM7 channel.
REFERENCE   10 (bases 1 to 7118)
  AUTHORS   Runnels,L.W., Yue,L. and Clapham,D.E.
  TITLE     TRP-PLIK, a bifunctional protein with kinase and ion channel
            activities
  JOURNAL   Science 291 (5506), 1043-1047 (2001)
   PUBMED   11161216
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On or before Sep 7, 2012 this sequence version replaced
            XM_001056331.4, XM_002726172.1, NM_053705.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.660399.1,
                                           SRR26643295.15233.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-254               JAXUCZ010000003.1  134587860-134588113 c
            255-334             JAXUCZ010000003.1  134571279-134571358 c
            335-373             JAXUCZ010000003.1  134567282-134567320 c
            374-572             JAXUCZ010000003.1  134564050-134564248 c
            573-786             JAXUCZ010000003.1  134561723-134561936 c
            787-911             JAXUCZ010000003.1  134557355-134557479 c
            912-1083            JAXUCZ010000003.1  134555133-134555304 c
            1084-1258           JAXUCZ010000003.1  134553182-134553356 c
            1259-1382           JAXUCZ010000003.1  134551890-134552013 c
            1383-1455           JAXUCZ010000003.1  134549773-134549845 c
            1456-1556           JAXUCZ010000003.1  134544621-134544721 c
            1557-1691           JAXUCZ010000003.1  134542826-134542960 c
            1692-1745           JAXUCZ010000003.1  134540678-134540731 c
            1746-1886           JAXUCZ010000003.1  134538211-134538351 c
            1887-2021           JAXUCZ010000003.1  134536680-134536814 c
            2022-2302           JAXUCZ010000003.1  134535562-134535842 c
            2303-2531           JAXUCZ010000003.1  134533655-134533883 c
            2532-2687           JAXUCZ010000003.1  134532616-134532771 c
            2688-2831           JAXUCZ010000003.1  134532395-134532538 c
            2832-2960           JAXUCZ010000003.1  134529961-134530089 c
            2961-3239           JAXUCZ010000003.1  134527596-134527874 c
            3240-3414           JAXUCZ010000003.1  134526453-134526627 c
            3415-3541           JAXUCZ010000003.1  134525155-134525281 c
            3542-3726           JAXUCZ010000003.1  134522360-134522544 c
            3727-3859           JAXUCZ010000003.1  134520280-134520412 c
            3860-4572           JAXUCZ010000003.1  134518530-134519242 c
            4573-4640           JAXUCZ010000003.1  134515888-134515955 c
            4641-4731           JAXUCZ010000003.1  134513987-134514077 c
            4732-4799           JAXUCZ010000003.1  134512246-134512313 c
            4800-4834           JAXUCZ010000003.1  134510330-134510364 c
            4835-4860           JAXUCZ010000003.1  134508849-134508874 c
            4861-4911           JAXUCZ010000003.1  134507246-134507296 c
            4912-4977           JAXUCZ010000003.1  134507102-134507167 c
            4978-5261           JAXUCZ010000003.1  134505743-134506026 c
            5262-5344           JAXUCZ010000003.1  134505563-134505645 c
            5345-5550           JAXUCZ010000003.1  134505218-134505423 c
            5551-5602           JAXUCZ010000003.1  134503588-134503639 c
            5603-5709           JAXUCZ010000003.1  134503377-134503483 c
            5710-7118           JAXUCZ010000003.1  134499617-134501025 c
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..7118
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /note="transient receptor potential cation channel,
                     subfamily M, member 7"
                     /db_xref="GeneID:679906"
                     /db_xref="RGD:620053"
     exon            1..254
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..5840
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /EC_number="2.7.11.1"
                     /note="long transient receptor potential channel 7;
                     transient receptor potential-phospholipase C-interacting
                     kinase"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 7"
                     /protein_id="NP_446157.2"
                     /db_xref="GeneID:679906"
                     /db_xref="RGD:620053"
                     /translation="MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFC
                     GRLVKQHACFTASLATKYSDVKLGEHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSH
                     SYRAKYVRLSYDTKPEIILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGL
                     IKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVG
                     RDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIH
                     ARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEE
                     GGNLPDAAEPDIISTIKKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDID
                     VAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMD
                     RVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLIRDVKQGNLPPGYKIT
                     LIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNASSSTPQLRKSHETFG
                     NRADKKEKMRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNE
                     LLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQY
                     SNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQ
                     MLLSDMWMGRLNMRKNSWYKVILSILVPPAILMLEYKTKAEMSHIPQSQDAHQMTMED
                     SENNFHNITEEIPMEVFKEVKILDSSEGKNEMEIHIKSKKLPITRKFYAFYHAPIVKF
                     WFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKIREVFMSEAGKISQ
                     KIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFW
                     YVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSW
                     SLAKDIVFHPYWMIFGEVYAYEIDVCANDSALPTICGPGTWLTPFLQAVYLFVQYIIM
                     VNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCIC
                     KRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFNSGSEERIRVTFERV
                     EQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHN
                     EITRELSISKHLAQNLIDDVPVRPMWKKPSVVNTLSSSLPQGDRESNNPFLCNIFMKD
                     EKDPQYNLFGQDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFN
                     KNQKLGSSPNSSPHMSSPPTKFSVSTPSQPSCKSHLESTTKDPEPIFYKAAEGDNIEF
                     GAFVGHRDSMDLQRFKETSNKIRELLSNDTPENTLKHVGAAGYNECHKTPTSLHSEQE
                     SCSRRASTEDSPDVDSRAALLPDWLRDRPTNREMPSEGGTLNGLASPFKPVLDTNYYY
                     SAVERNNLMRLSQSIPFVPVPPRGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIEF
                     LSKEEMGGGLRRAVKVLCTWSEHDVLRSGHLYIIKSFLPEVINTWSSIYKEDTVLHLC
                     LREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRK
                     YNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKR
                     SCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDESSDLN
                     LQAGNSTKESEATNSVRLML"
     exon            255..334
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            335..373
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            374..572
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            573..786
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            787..911
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1083
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1258
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1382
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1455
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1556
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1691
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1745
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1886
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2021
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2302
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2531
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2687
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2831
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2960
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3239
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            3240..3414
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3541
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3726
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            3727..3859
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            3860..4572
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4640
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4641..4731
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4732..4799
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4800..4834
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4835..4860
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4861..4911
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4912..4977
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            4978..5261
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            5262..5344
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            5345..5550
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            5551..5602
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            5603..5709
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
     exon            5710..7118
                     /gene="Trpm7"
                     /gene_synonym="Chak; LTrpC-7; Ltrpc7; Trp-plik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgtctccgg cggaggcggg cgcgggcgcg tccctgtggc cagtcacccg gcggagctgg
       61 tcgcacaatt atgaaagact cgacttctgc tgctagcgct ggagctgagt tagttctgag
      121 aaggtttccc ggggctgtcc ttgttcggtg gcccgtgcca ccgcctccgg agacgctttc
      181 cgatagatgg ctgcaggccg cggaggagga ggaggagccg ctgcccttcc ggagtccgcc
      241 ccttgaggag aatgtcccag aaatcctgga tagagagcac cttgaccaag agggagtgtg
      301 tgtatattat accaagctcc aaagaccctc acagatgtct tccaggatgt cagatttgtc
      361 agcaacttgt cagatgtttc tgtggtcgtt tggtcaagca gcatgcatgc tttactgcaa
      421 gtcttgccac aaaatactca gatgtgaaat tgggtgaaca ctttaaccag gcaatagaag
      481 aatggtctgt ggaaaagcac acggagcaga gcccaacaga tgcttatgga gtcatcaatt
      541 ttcaaggggg ttctcattcc tacagagcta agtatgtgag actatcatat gataccaaac
      601 ctgaaatcat tctgcaactt ctgcttaaag aatggcaaat ggagttaccc aaacttgtta
      661 tttctgtaca tggaggcatg cagaaatttg aacttcatcc aagaatcaag cagttgcttg
      721 gaaagggtct tattaaagct gcagttacaa ccggagcttg gattttaact ggaggagtaa
      781 atacaggtgt ggcaaaacat gttggtgatg ccctcaaaga acatgcttcc agatcatctc
      841 gaaaaatttg cactattgga atagctccat ggggagtgat agaaaacagg aatgatcttg
      901 ttgggagaga tgtggttgcc ccctatcaaa ccctattaaa tcccttgagc aaattgaatg
      961 ttctgaataa tctacactcc catttcatct tggtggatga cggcacggtt ggaaagtatg
     1021 gggcagaagt cagactgaga agagaacttg aaaaaaccat taatcagcaa agaattcatg
     1081 ctagaattgg gcaaggtgtt cctgtggtgg ctttgatatt tgaaggcggg ccaaatgtca
     1141 tccttacagt actggagtac ctccaggaaa gcccccccgt tccagttgtt gtgtgtgaag
     1201 ggacaggcag agctgcagat ttactagcat atatccataa gcagacagag gaaggaggga
     1261 atcttcctga tgcagcagag cctgatatta tatcaactat caaaaaaaca tttaactttg
     1321 gccagagtga agcagttcat ttatttcaaa caatgatgga gtgtatgaaa aaaaaagagc
     1381 ttatcactgt tttccatatt ggttcagagg accatcaaga tatagatgtg gccatactta
     1441 ctgcactgct aaaaggtact aatgcatcag cgtttgacca gcttatcctt acactggcat
     1501 gggacagagt tgatattgcc aaaaatcatg tatttgttta tggacaacag tggttggttg
     1561 gatccttgga acaagctatg cttgatgctc ttgtaatgga tagagtttca tttgtaaaac
     1621 ttcttattga aaatggagta agcatgcata aattccttac cattcctaga ctggaagaac
     1681 tttataacac taaacagggt ccgactaatc caatgttgtt ccacctcatt cgggatgtca
     1741 aacagggtaa tcttcctcca gggtacaaga tcactctaat tgatatagga cttgtgattg
     1801 aatatctcat gggaggaacc tacagatgca catacacacg aaaacgtttt cgcttaatat
     1861 ataatagtct tggtggaaat aaccggaggt caggtcgaaa tgcttccagc agcacccctc
     1921 agttgcgaaa gagtcatgaa acttttggca atagagctga taaaaaggaa aaaatgagac
     1981 acaatcattt cattaaaaca gcccaaccct acagaccaaa gatggatgca tctatggaag
     2041 aagggaagaa gaaacgaacc aaagatgaaa ttgtagatat agatgatcca gaaaccaagc
     2101 gctttcctta tcctcttaat gaattattga tctgggcttg ccttatgaag aggcaagtca
     2161 tggcccgctt tttatggcag catggtgagg aatcaatggc taaagcatta gttgcctgta
     2221 aaatctatcg ttcaatggct tatgaggcaa aacagagtga cctggtagat gatacttcag
     2281 aggaactgaa acagtattcc aatgattttg gtcaactcgc cgttgaatta ctggaacagt
     2341 ccttcagaca agatgaaaca atggctatga aattactcac ttatgaactg aaaaactgga
     2401 gtaattcaac ctgcctcaag ttagcagttt cttcaaggct taggcctttt gtagctcaca
     2461 cttgtacaca gatgctgttg tctgatatgt ggatgggacg gctgaatatg agaaaaaatt
     2521 cctggtataa ggttatatta agcattttag ttccacctgc catattaatg ctagagtata
     2581 aaaccaaggc tgaaatgtcc catatcccac aatctcaaga tgctcatcaa atgaccatgg
     2641 aggatagtga aaacaatttt cacaacataa cagaagagat acccatggaa gtatttaaag
     2701 aagtaaagat tttggacagc agtgaaggaa agaatgaaat ggagatacat attaaatcaa
     2761 aaaagcttcc aatcacacga aaattttatg ccttttatca cgcgccaatt gtaaagttct
     2821 ggtttaacac attggcatac ttaggatttc tgatgcttta tacatttgta gttcttgtac
     2881 aaatggaaca gttaccttca gttcaagagt ggattgttat cgcttatatt tttacctatg
     2941 ctattgaaaa aattcgtgag gtctttatgt ctgaagctgg gaaaatcagc cagaagatca
     3001 aagtgtggtt tagtgactac ttcaatgtca gtgacacaat tgccataatt tctttctttg
     3061 ttggatttgg actaagattt ggagcaaaat ggaactatat taatgcatat gataatcatg
     3121 tttttgttgc tggaagatta atttactgtc ttaatataat attttggtat gtgcgcttgc
     3181 tagattttct agctgtaaat caacaggcag gaccttatgt aatgatgatt ggaaaaatgg
     3241 tggccaatat gttctacatt gtagtgataa tggctcttgt attacttagt tttggtgttc
     3301 ccagaaaagc aatactttat ccacatgaag aaccgtcttg gtctcttgct aaagatatag
     3361 tttttcatcc atactggatg atttttggtg aagtttatgc gtatgaaatt gatgtgtgtg
     3421 caaatgattc cgctctcccg acaatctgtg gtcctggaac ttggttgact ccatttcttc
     3481 aagcagtcta cctctttgta cagtatatca ttatggttaa tcttcttatt gcatttttca
     3541 ataatgtata tttacaagtg aaggcaattt ccaatattgt atggaagtat cagcgatatc
     3601 atttcattat ggcttatcat gaaaaaccag tcctgcctcc tccacttatc attctcagcc
     3661 atatagtttc actgttttgc tgtatctgta aaagaagaaa gaaagataag acttctgatg
     3721 ggccaaaact tttcttaaca gaagaagatc aaaagaaact tcatgacttt gaagaacagt
     3781 gtgttgagat gtattttgat gagaaagatg acaaattcaa ttctggaagt gaagagagaa
     3841 tccgtgtcac ttttgaaaga gtggagcaga tgagcattca gattaaagaa gttggagatc
     3901 gtgtcaacta cataaaaaga tcgttacagt ctttagattc tcaaattggt cacctgcaag
     3961 atctttcagc cctaacagta gatacattaa aaacacttac agcccagaaa gcttcagaag
     4021 ctagcaaagt gcacaatgag attacacgag aattgagtat ttccaaacac ttggctcaga
     4081 atcttattga tgatgttcct gtaaggccta tgtggaagaa gcctagtgtt gtaaatacac
     4141 tgagttcctc tcttcctcaa ggtgatcggg aaagtaataa tccttttctt tgtaatattt
     4201 ttatgaaaga tgaaaaagac ccccagtata acctgtttgg acaggatttg cctgtgatac
     4261 cccagagaaa agaattcaac attccagagg ctggctcctc ctgtggtgcc ttattcccaa
     4321 gtgctgtttc tcccccagaa ttacgacaaa gacgacatgg ggtagaaatg ttaaaaatat
     4381 ttaataaaaa tcaaaaatta ggcagttcac ctaatagttc accccatatg tcctccccac
     4441 caaccaaatt ttctgtgagt accccatccc agccaagttg caaaagccac ttggaatcca
     4501 caaccaaaga tccagaaccc attttctata aagctgcaga aggggataac atagaatttg
     4561 gagcatttgt gggacacaga gatagtatgg atttacagag gtttaaagaa acatcaaaca
     4621 aaataagaga actgttatct aatgatactc ctgaaaacac tttgaaacat gtgggtgctg
     4681 ctggatataa tgaatgtcat aagactccta cttctcttca ttcagaacaa gagagctgta
     4741 gtagaagagc gtcgacagaa gactctccgg acgtagattc cagagcagct ttgttgccgg
     4801 attggttacg agatagacca acaaacagag aaatgccatc tgaaggagga acattaaatg
     4861 gtcttgcttc tccatttaaa cctgttttgg atacaaatta ctattattca gctgtggaaa
     4921 gaaataacct gatgaggctg tcgcagagta ttcccttcgt tcctgtacct ccacgaggtg
     4981 agcctgtcac agtatatcgt ctggaggaga gttctcccag tatactgaat aacagcatgt
     5041 cttcttggtc tcagctaggc ctctgtgcca aaattgagtt tttaagtaaa gaggaaatgg
     5101 gaggcggttt acgaagagca gtcaaagtgc tgtgtacctg gtcagagcac gatgtcctga
     5161 gatcagggca cctttatatc attaagtcgt ttcttccaga ggtgataaac acatggtcaa
     5221 gcatttataa agaagacaca gttctgcatc tctgtctcag agaaatacaa caacagagag
     5281 cagcacagaa gctcacattt gcctttaatc agatgaaacc caaatccata ccatattctc
     5341 caaggttcct ggaagttttc ctgctatact gccattcagc agggcagtgg tttgctgtag
     5401 aagaatgcat gactggggaa tttaggaaat acaacaataa taatggtgat gaaatcattc
     5461 caaccaacac tctggaagag atcatgctag ccttcagcca ctggacctat gagtacacca
     5521 gaggggagtt gctggtactt gacttacaag gtgtgggaga aaacttgact gacccatctg
     5581 tgataaaagc tgaagagaaa agatcctgcg acatggtttt tggccctgcc aatttaggag
     5641 aagatgcaat aaaaaacttc agagccaaac atcactgtaa ttcttgctgt cgaaagctta
     5701 aacttccaga tctgaagagg aatgactaca cacctgataa aattatattt cctcaggatg
     5761 agtcatcaga tctgaatctt caagctggaa attctaccaa agaatcagaa gcaacaaatt
     5821 ctgttcgtct gatgttatag tactgaacca ttgatctttg cctacatttc acaaaagtgt
     5881 aactgacgtt tttcctttca ggggggaatt gatggtatag gaaggtgtgt gcaaaatgag
     5941 cttgctggcc ccacacatag ttcagaggta atgttctcat tgaaaagcac ctcaaggcta
     6001 cagatgaccg ctgggaaatg ttaacagtga cagaaagtta gtgctctcgg cttgtgaagg
     6061 acctgaagct ttgctgttga gggtggtgat tctctgtcac ttggtgcagt accattaacc
     6121 aaagtcaagt gtcttcagat tttattggct gctcagttac agccattcag ctaaagaatc
     6181 taaattatgc agcttttcgg ttaagtggct gaagtcttcc tcagtttgtg ctctctgata
     6241 atgatgttag ctctcaacta gatatttgtg gtcacaggaa aactattcct tacaattttg
     6301 cttcacaggc atgttacaaa gcctgcacta aaaaccattt gtcttccctc cctccctctc
     6361 tttttcctgt agtgtagaag atcaaatcca gggcctcatg aagaccattt tctaagaaac
     6421 attttaagaa tcaactttag agtctttgtt tatggataca gccagttttt gttaaacaaa
     6481 actgaattgt gcaaaagggt tttttttttt aacatttatc aatgttaagt aaaagaaagc
     6541 catgataaat aaggattaac tcactgttca gtgggtgttc cctgtgagga aggttgcagt
     6601 tgtaacagcc tgcagctgca tacatcccca aagatttaca gacttagtgt atcaaatcag
     6661 agtgtcatgt gaactctcac atttaaaatt ctataggaat gtgtcaatgt gaattctatg
     6721 tctggtactt atttaagaaa tcagttgttg gattatcctt atatagtata gggagatcac
     6781 aatacaactt tatgccaata aaataactta attgcccaga tatttttgca tatttagcaa
     6841 caagaaaagc ttatcatttg actcaagttt tatgctttct ctttcttttc atttcctagg
     6901 tactaatttt aatttttatt tggaagggag cagtgtaaag cttatttgta ttcaatagtg
     6961 tatctcatag atacagacaa ggccgcagag ataagctgtt aaatagtgtt taaagttgat
     7021 gtggagagaa aaggtgtatt acttaaagat actataccat atatgttttg tatatcatta
     7081 aatctttaaa agaaattaaa tttattcttg tttacaga
//
