LOCUS       NP_997491               1125 aa            linear   ROD 27-JUL-2024
DEFINITION  transient receptor potential cation channel subfamily A member 1
            [Rattus norvegicus].
ACCESSION   NP_997491 XP_232586
VERSION     NP_997491.2
DBSOURCE    REFSEQ: accession NM_207608.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1125)
  AUTHORS   Rumbus,Z., Fekete,K., Kelava,L., Gardos,B., Klonfar,K.,
            Keringer,P., Pinter,E., Pakai,E. and Garami,A.
  TITLE     Ammonium chloride-induced hypothermia is attenuated by transient
            receptor potential channel vanilloid-1, but augmented by ankyrin-1
            in rodents
  JOURNAL   Life Sci 346, 122633 (2024)
   PUBMED   38615746
  REMARK    GeneRIF: Ammonium chloride-induced hypothermia is attenuated by
            transient receptor potential channel vanilloid-1, but augmented by
            ankyrin-1 in rodents.
REFERENCE   2  (residues 1 to 1125)
  AUTHORS   Kozyreva,T.V., Orlov,I.V., Boyarskaya,A.R. and Voronova,I.P.
  TITLE     Hypothalamic TRPM8 and TRPA1 ion channel genes in the regulation of
            temperature homeostasis at water balance changes
  JOURNAL   Neurosci Lett 828, 137763 (2024)
   PUBMED   38574849
  REMARK    GeneRIF: Hypothalamic TRPM8 and TRPA1 ion channel genes in the
            regulation of temperature homeostasis at water balance changes.
REFERENCE   3  (residues 1 to 1125)
  AUTHORS   Imamura,T., Ogawa,T., Minagawa,T., Daimon,H., Nagai,T., Ueno,M.,
            Saito,T. and Ishizuka,O.
  TITLE     Transient receptor potential ankyrin 1 channels in the bladder
            mediate low temperature elicited bladder overactivity in rats
  JOURNAL   Neurourol Urodyn 43 (1), 276-288 (2024)
   PUBMED   38010891
  REMARK    GeneRIF: Transient receptor potential ankyrin 1 channels in the
            bladder mediate low temperature elicited bladder overactivity in
            rats.
REFERENCE   4  (residues 1 to 1125)
  AUTHORS   Nakagawa,M., Takahashi,K., Nishizawa,Y. and Ohta,T.
  TITLE     Involvement of interaction of Cav3.2 and nociceptive TRPA1 in
            pathological pain transmission
  JOURNAL   Biomed Res 45 (1), 45-55 (2024)
   PUBMED   38325845
  REMARK    GeneRIF: Involvement of interaction of Cav3.2 and nociceptive TRPA1
            in pathological pain transmission.
REFERENCE   5  (residues 1 to 1125)
  AUTHORS   Skagen,C., Lovsletten,N.G., Asoawe,L., Al-Karbawi,Z., Rustan,A.C.,
            Thoresen,G.H. and Haugen,F.
  TITLE     Functional expression of the thermally activated transient receptor
            potential channels TRPA1 and TRPM8 in human myotubes
  JOURNAL   J Therm Biol 116, 103623 (2023)
   PUBMED   37542841
  REMARK    GeneRIF: Functional expression of the thermally activated transient
            receptor potential channels TRPA1 and TRPM8 in human myotubes.
REFERENCE   6  (residues 1 to 1125)
  AUTHORS   Obata,K., Katsura,H., Mizushima,T., Yamanaka,H., Kobayashi,K.,
            Dai,Y., Fukuoka,T., Tokunaga,A., Tominaga,M. and Noguchi,K.
  TITLE     TRPA1 induced in sensory neurons contributes to cold hyperalgesia
            after inflammation and nerve injury
  JOURNAL   J Clin Invest 115 (9), 2393-2401 (2005)
   PUBMED   16110328
  REMARK    GeneRIF: Results demonstrate that nerve growth factor-induced TRPA1
            increase in sensory neurons via p38 activation is necessary for
            cold hyperalgesia.
            Erratum:[J Clin Invest. 2010 Jan;120(1):394]
REFERENCE   7  (residues 1 to 1125)
  AUTHORS   Nagata,K., Duggan,A., Kumar,G. and Garcia-Anoveros,J.
  TITLE     Nociceptor and hair cell transducer properties of TRPA1, a channel
            for pain and hearing
  JOURNAL   J Neurosci 25 (16), 4052-4061 (2005)
   PUBMED   15843607
REFERENCE   8  (residues 1 to 1125)
  AUTHORS   Corey,D.P., Garcia-Anoveros,J., Holt,J.R., Kwan,K.Y., Lin,S.Y.,
            Vollrath,M.A., Amalfitano,A., Cheung,E.L., Derfler,B.H., Duggan,A.,
            Geleoc,G.S., Gray,P.A., Hoffman,M.P., Rehm,H.L., Tamasauskas,D. and
            Zhang,D.S.
  TITLE     TRPA1 is a candidate for the mechanosensitive transduction channel
            of vertebrate hair cells
  JOURNAL   Nature 432 (7018), 723-730 (2004)
   PUBMED   15483558
REFERENCE   9  (residues 1 to 1125)
  AUTHORS   Jordt,S.E., Bautista,D.M., Chuang,H.H., McKemy,D.D., Zygmunt,P.M.,
            Hogestatt,E.D., Meng,I.D. and Julius,D.
  TITLE     Mustard oils and cannabinoids excite sensory nerve fibres through
            the TRP channel ANKTM1
  JOURNAL   Nature 427 (6971), 260-265 (2004)
   PUBMED   14712238
  REMARK    GeneRIF: cellular and molecular target for the pungent action of
            mustard oils; support an emerging role for TRP channel ANKTM1 as an
            ionotropic cannabinoid receptor.
REFERENCE   10 (residues 1 to 1125)
  AUTHORS   Story,G.M., Peier,A.M., Reeve,A.J., Eid,S.R., Mosbacher,J.,
            Hricik,T.R., Earley,T.J., Hergarden,A.C., Andersson,D.A.,
            Hwang,S.W., McIntyre,P., Jegla,T., Bevan,S. and Patapoutian,A.
  TITLE     ANKTM1, a TRP-like channel expressed in nociceptive neurons, is
            activated by cold temperatures
  JOURNAL   Cell 112 (6), 819-829 (2003)
   PUBMED   12654248
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Aug 30, 2022 this sequence version replaced NP_997491.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY496961.1, SRR26360176.483863.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q11"
     Protein         1..1125
                     /product="transient receptor potential cation channel
                     subfamily A member 1"
                     /note="ankyrin-like with transmembrane domains protein 1;
                     wasabi receptor"
                     /calculated_mol_wt=128473
     Region          47..337
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          63..94
                     /region_name="ANK 1. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            order(98,100,104..105,108..110,112..113,117,120,129,131,
                     133,137..138,141..143,145..146,150,153,163,165,167,
                     171..172,175..177,179..180,184,187)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          98..129
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          98..127
                     /region_name="ANK 2. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          131..163
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          131..161
                     /region_name="ANK 3. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          165..194
                     /region_name="ANK 4. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          165..190
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          166..219
                     /region_name="Ank_4"
                     /note="Ankyrin repeats (many copies); pfam13637"
                     /db_xref="CDD:372654"
     Region          198..237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          198..227
                     /region_name="ANK 5. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          239..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          239..268
                     /region_name="ANK 6. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          272..301
                     /region_name="ANK 7. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          276..307
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(278..279,282..284,286..287,291,294,307,309,311,
                     315..316,319..321,323..324,328,331,340,342,344,348..349,
                     352..354,356..357,361,364,373)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          301..582
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          309..340
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          309..338
                     /region_name="ANK 8. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          342..373
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          342..371
                     /region_name="ANK 9. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            395
                     /site_type="hydroxylation"
                     /note="4-hydroxyproline, transient.
                     /evidence=ECO:0000250|UniProtKB:O75762; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          413..444
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          413..442
                     /region_name="ANK 10. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          446..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          446..475
                     /region_name="ANK 11. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            order(482,484,488..489,492..494,496..497,501,504,512,514,
                     516,520..521,524..526,528..529,533,536,546,548,550,
                     554..555,558..560,562..563,567,570)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          482..512
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          482..511
                     /region_name="ANK 12. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          514..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          514..543
                     /region_name="ANK 13. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          548..577
                     /region_name="ANK 14. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          548..573
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            622
                     /site_type="other"
                     /note="Essential for electrophile activation. Sensor for
                     electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:O75762; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            623
                     /site_type="other"
                     /note="Key residue for activation by the scorpion wasabi
                     receptor toxin. /evidence=ECO:0000250|UniProtKB:O75762;
                     propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            635
                     /site_type="other"
                     /note="Important residue for activation by the scorpion
                     wasabi receptor toxin.
                     /evidence=ECO:0000250|UniProtKB:O75762; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            647
                     /site_type="other"
                     /note="Important residue for activation by the scorpion
                     wasabi receptor toxin.
                     /evidence=ECO:0000250|UniProtKB:O75762; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            722..742
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            749
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            755
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            768..788
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Region          775..975
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Site            807..827
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            833..853
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            877..897
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            918
                     /site_type="other"
                     /note="Crucial for calcium permeation.
                     /evidence=ECO:0000269|PubMed:18775987; propagated from
                     UniProtKB/Swiss-Prot (Q6RI86.2)"
     Site            938..959
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6RI86.2)"
     CDS             1..1125
                     /gene="Trpa1"
                     /gene_synonym="Anktm1"
                     /coded_by="NM_207608.2:138..3515"
                     /db_xref="GeneID:312896"
                     /db_xref="RGD:1303284"
ORIGIN      
        1 mkrslrrvlr peerkevqgv vyrgvgedmd cskesfkvdi egdmcrleaf iknrrklsky
       61 edenlcllhh aaaegqvelm qliingssce alnvmddygn tplhwaaekn qvesvkflls
      121 qganpnlrnr nmmaplhiav qgmynevikv ltehkatnin legengntal mstcakdnse
      181 alqillekga klcksnkwgd ypvhqaafsg akrcmelila ygektgysre ahinfvnhkk
      241 asplhlavqs gdldmikmcl dsgahidmme nakcmalhfa atqgatdivk lmissytgss
      301 divnavdgnq etllhraslf dhhdladyli svgadinstd segrsplila tasaswnivn
      361 lllskgakvd ikdhlgrnfl hltvqqpygl rnlrpeflqm qhikelvmde dndgctplhy
      421 acrqgapvsv nnllrfnvsv hskskdkksp lhfaasygri ntcqrllqdi sdtrllnegd
      481 lhgmtplhla aknghdkvvq lllkkgalfl sdhngwtalh hasmggytqt mkvildtnlk
      541 ctdrldeegn talhfaareg hakavamlls ynadillnkk qasflhialh nkrkevvltt
      601 irskrwdecl qvfthdspsn rcpimemvey lpecmkvlld fcmipstedk scqdyhieyn
      661 fkylqcplsm tkkvtptqdv iyepltilnv mvqhnriell nhpvcreyll mkwcaygfra
      721 hmmnlgsycl glipmtllvv kiqpgmafns tgiinetist heerintlns fplkicmilv
      781 flssifgyck evvqifqqkr nyfldynnal ewviyttsmi fvlplfldip aymqwqcgai
      841 aiffywmnfl lylqrfencg ifivmlevif ktllrstgvf iflllafgls fyvllnfqda
      901 fstpllsliq tfsmmlgdin yrdafleplf rnelaypvlt fgqliaftmf vpivlmnlli
      961 glavgdiaev qkhaslkria mqvelhtnle kklpfwylrk vdqrstivyp nrprhgrmlr
     1021 ffhyflsmqe trqeapnidt clemeilkqk yrlkdltsll ekqhelikli iqkmeiiset
     1081 edednhcsfq drfkkerleq mhskwnfvln avktkthcsi shpdi
//
