LOCUS       NM_001401175            1626 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus tRNA mitochondrial 2-thiouridylase (Trmu),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001401175 XM_006242164
VERSION     NM_001401175.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Gaignard,P., Gonzales,E., Ackermann,O., Labrune,P., Correia,I.,
            Therond,P., Jacquemin,E. and Slama,A.
  TITLE     Mitochondrial Infantile Liver Disease due to TRMU Gene Mutations:
            Three New Cases
  JOURNAL   JIMD Rep 11, 117-123 (2013)
   PUBMED   23625533
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Zeharia,A., Shaag,A., Pappo,O., Mager-Heckel,A.M., Saada,A.,
            Beinat,M., Karicheva,O., Mandel,H., Ofek,N., Segel,R., Marom,D.,
            Rotig,A., Tarassov,I. and Elpeleg,O.
  TITLE     Acute infantile liver failure due to mutations in the TRMU gene
  JOURNAL   Am J Hum Genet 85 (3), 401-407 (2009)
   PUBMED   19732863
  REMARK    Erratum:[Am J Hum Genet. 2010 Feb;86(2):295]
REFERENCE   3  (bases 1 to 1626)
  AUTHORS   Yan,Q. and Guan,M.X.
  TITLE     Identification and characterization of mouse TRMU gene encoding the
            mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase
  JOURNAL   Biochim Biophys Acta 1676 (2), 119-126 (2004)
   PUBMED   14746906
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Feb 3, 2022 this sequence version replaced XM_006242164.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC161991.1, SRR26360194.1221317.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               JAXUCZ010000007.1  118849604-118849742
            140-305             JAXUCZ010000007.1  118852910-118853075
            306-412             JAXUCZ010000007.1  118854997-118855103
            413-535             JAXUCZ010000007.1  118858753-118858875
            536-708             JAXUCZ010000007.1  118861272-118861444
            709-901             JAXUCZ010000007.1  118862540-118862732
            902-1002            JAXUCZ010000007.1  118863697-118863797
            1003-1147           JAXUCZ010000007.1  118865107-118865251
            1148-1230           JAXUCZ010000007.1  118865609-118865691
            1231-1626           JAXUCZ010000007.1  118865795-118866190
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1626
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /note="tRNA mitochondrial 2-thiouridylase"
                     /db_xref="GeneID:362976"
                     /db_xref="RGD:1311229"
     exon            1..139
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1383
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /EC_number="2.8.1.14"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mitochondrial tRNA-specific 2-thiouridylase 1; tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase 1;
                     tRNA 5-methylaminomethyl-2-thiouridylate
                     methyltransferase"
                     /codon_start=1
                     /product="mitochondrial tRNA-specific 2-thiouridylase 1
                     isoform 2"
                     /protein_id="NP_001388104.1"
                     /db_xref="GeneID:362976"
                     /db_xref="RGD:1311229"
                     /translation="MSALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDE
                     QGICAADKDCEDAYKVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDISCN
                     KHIKFSCFHHYAVDNLGADAVATGHYARTSLEDEEVFEQKHTKRPDGLFRNRFEVRNP
                     VKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGELTKDFVKKIAAENRLHHVLQKKES
                     MGICFIGKRNLEHFLLQVSVSDVSGGLLWAGAPVVMKPVFQYLQPRPGKFISIEDNRV
                     LGTHKGWFLYTLGQRAKISGLSEPWYVVEKDGTKGDVLVAPRVDHPALYRDLLRTNRV
                     HWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVKAVRGLALGQF
                     AVFYKGEECLGSGKILRLGPSAYTLQKGKNRTRVAPEVSSDSPGLHPTS"
     exon            140..305
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..412
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            413..535
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..708
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            709..901
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1002
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1147
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1230
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1626
                     /gene="Trmu"
                     /gene_synonym="Mtu1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tccggcgtat cgtgcacgca gccagaaagc tgctgggagg agttgcggtg tgtgcagatg
       61 tcggcgttgc ggcacgtcgt gtgtgccctg tctggcggcg tggacagcgc ggtggccgcg
      121 ctgctgttac ggcggagagg ttaccaggtg acaggggtat ttatgaagaa ctgggactcg
      181 ctggatgagc agggcatttg tgctgctgac aaggactgtg aagatgcgta taaagtgtgt
      241 cagatcttag acatcccttt ccaccaggtg tcctatgtga aggagtattg gaatgatgta
      301 ttcagtgact ttctgaacga gtatgagaag ggaaggaccc cgaaccccga catcagctgc
      361 aacaagcaca tcaagttcag ctgctttcac cattatgctg tggacaatct tggagcagat
      421 gctgttgcca ctggccacta tgcaaggacg tccctggagg atgaggaagt ctttgagcaa
      481 aagcacacta agagaccaga tggactgttc aggaatcgat ttgaagtcag aaatcccgta
      541 aaacttctcc aagcagctga cagctttaaa gaccagacct tctttctcag ccaggtttcc
      601 caggatgccc tgagaagaac catcttcccc ctgggggaat taacaaaaga ttttgtaaag
      661 aaaatagctg cggagaatag actccatcat gtgcttcaga agaaagagag catgggcatc
      721 tgcttcattg gtaagagaaa cctggagcat ttccttcttc aggtgagtgt gtctgacgtg
      781 agtggcggtc tgctctgggc tggggctcca gtagtgatga aacctgtctt ccagtatttg
      841 cagcctcggc ctgggaagtt tatttctatc gaggataaca gagttctggg aacacacaaa
      901 ggttggttcc tatacacatt gggccagaga gctaagatca gcggcttgag cgagccctgg
      961 tacgtggtgg agaaggatgg caccaagggt gacgtgcttg tggctccccg ggtagaccat
     1021 ccagctctgt acagggacct gctgaggacc aaccgagtgc actggatagc tgaggaacca
     1081 cctgcagcct tggtcagaga caagatgatg gagtgccact tccgattccg ccaccagatg
     1141 gcactagtgc cctgtgtgct gacgctcaac caggatggca ctgtgtgggt gacggctgtg
     1201 aaggctgtga ggggccttgc cctgggacag tttgcagtgt tctacaaggg tgaggaatgc
     1261 ctcggcagtg ggaaaatcct gcggttaggg ccatctgcct acacactcca gaagggcaag
     1321 aacagaacca gagtggctcc tgaggtctcc agtgacagcc caggcctgca ccccacatcc
     1381 tgacaatgac agaatctgca gcaggatggt cagtgatggg ctttggtgtg gccagaaaca
     1441 gtgggcaggc tggctgcagt cagggcagcc tgaggaggga gtgtctgcgc tgctactcca
     1501 ggaaaccaga agtacgagct ggcaccgggt ctgcctcaag ggccctgatg gggctgctct
     1561 tccacagtgt ccagagaagg ccagcacagg aagagcagca gcaaataaaa ctctgtcaaa
     1621 gtctga
//
