LOCUS       NM_001107658           11401 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus trio Rho guanine nucleotide exchange factor
            (Trio), mRNA.
ACCESSION   NM_001107658 XM_001058824 XM_003749226 XM_003753540 XM_226888
VERSION     NM_001107658.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Paskus,J.D., Tian,C., Fingleton,E., Shen,C., Chen,X., Li,Y.,
            Myers,S.A., Badger,J.D. 2nd, Bemben,M.A., Herring,B.E. and
            Roche,K.W.
  TITLE     Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF
            Protein-Dependent Neuroligin-1 Mechanism of Action
  JOURNAL   Cell Rep 29 (10), 2944-2952 (2019)
   PUBMED   31801062
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Sadybekov,A., Tian,C., Arnesano,C., Katritch,V. and Herring,B.E.
  TITLE     An autism spectrum disorder-related de novo mutation hotspot
            discovered in the GEF1 domain of Trio
  JOURNAL   Nat Commun 8 (1), 601 (2017)
   PUBMED   28928363
  REMARK    GeneRIF: In accordance with pathological increases or decreases in
            glutamatergic neurotransmission observed in animal models of autism
            spectrum disorder, we find that Trio mutations result in either
            reduced synaptic AMPA receptor expression or enhanced glutamatergic
            synaptogenesis. Together, our findings implicate both excessive and
            reduced Trio activity and the resulting synaptic dysfunction in
            ASD-related pathogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11401)
  AUTHORS   Terry-Lorenzo,R.T., Torres,V.I., Wagh,D., Galaz,J., Swanson,S.K.,
            Florens,L., Washburn,M.P., Waites,C.L., Gundelfinger,E.D.,
            Reimer,R.J. and Garner,C.C.
  TITLE     Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone
            Proteins Piccolo and Bassoon
  JOURNAL   PLoS One 11 (12), e0167535 (2016)
   PUBMED   27907191
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11401)
  AUTHORS   Ba,W., Yan,Y., Reijnders,M.R., Schuurs-Hoeijmakers,J.H.,
            Feenstra,I., Bongers,E.M., Bosch,D.G., De Leeuw,N., Pfundt,R.,
            Gilissen,C., De Vries,P.F., Veltman,J.A., Hoischen,A.,
            Mefford,H.C., Eichler,E.E., Vissers,L.E., Nadif Kasri,N. and De
            Vries,B.B.
  TITLE     TRIO loss of function is associated with mild intellectual
            disability and affects dendritic branching and synapse function
  JOURNAL   Hum Mol Genet 25 (5), 892-902 (2016)
   PUBMED   26721934
  REMARK    GeneRIF: TRIO loss of function is associated with mild intellectual
            disability and affects dendritic branching and synapse function.
REFERENCE   5  (bases 1 to 11401)
  AUTHORS   Herring,B.E. and Nicoll,R.A.
  TITLE     Kalirin and Trio proteins serve critical roles in excitatory
            synaptic transmission and LTP
  JOURNAL   Proc Natl Acad Sci U S A 113 (8), 2264-2269 (2016)
   PUBMED   26858404
REFERENCE   6  (bases 1 to 11401)
  AUTHORS   Liu,X.F., Bera,T.K., Kahue,C., Escobar,T., Fei,Z., Raciti,G.A. and
            Pastan,I.
  TITLE     ANKRD26 and its interacting partners TRIO, GPS2, HMMR and DIPA
            regulate adipogenesis in 3T3-L1 cells
  JOURNAL   PLoS One 7 (5), e38130 (2012)
   PUBMED   22666460
REFERENCE   7  (bases 1 to 11401)
  AUTHORS   Neubrand,V.E., Thomas,C., Schmidt,S., Debant,A. and Schiavo,G.
  TITLE     Kidins220/ARMS regulates Rac1-dependent neurite outgrowth by direct
            interaction with the RhoGEF Trio
  JOURNAL   J Cell Sci 123 (Pt 12), 2111-2123 (2010)
   PUBMED   20519585
  REMARK    GeneRIF: Kidins220 regulates Rac1-dependent neurite outgrowth by
            direct interaction with the Trio.
REFERENCE   8  (bases 1 to 11401)
  AUTHORS   McPherson,C.E., Eipper,B.A. and Mains,R.E.
  TITLE     Multiple novel isoforms of Trio are expressed in the developing rat
            brain
  JOURNAL   Gene 347 (1), 125-135 (2005)
   PUBMED   15715966
REFERENCE   9  (bases 1 to 11401)
  AUTHORS   Ma,X.M., Huang,J.P., Eipper,B.A. and Mains,R.E.
  TITLE     Expression of Trio, a member of the Dbl family of Rho GEFs in the
            developing rat brain
  JOURNAL   J Comp Neurol 482 (4), 333-348 (2005)
   PUBMED   15669055
  REMARK    GeneRIF: Trio levels in the hippocampus and cortex are high at P1,
            decreasing until P30. The data suggest that Trio plays an important
            role during neuronal development.
REFERENCE   10 (bases 1 to 11401)
  AUTHORS   Xin,X., Ferraro,F., Back,N., Eipper,B.A. and Mains,R.E.
  TITLE     Cdk5 and Trio modulate endocrine cell exocytosis
  JOURNAL   J Cell Sci 117 (Pt 20), 4739-4748 (2004)
   PUBMED   15331630
  REMARK    GeneRIF: Data suggest that control of the cortical actin
            cytoskeleton, long known to modulate hormone exocytosis and
            subsequent endocytosis, involves cyclin-dependent kinase 5
            (Cdk5)-mediated activation of Trio.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Mar 16, 2021 this sequence version replaced NM_001107658.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-442               JAXUCZ010000002.1  80531171-80531612   c
            443-517             JAXUCZ010000002.1  80430129-80430203   c
            518-632             JAXUCZ010000002.1  80423221-80423335   c
            633-825             JAXUCZ010000002.1  80416081-80416273   c
            826-1338            JAXUCZ010000002.1  80413412-80413924   c
            1339-1461           JAXUCZ010000002.1  80412166-80412288   c
            1462-1653           JAXUCZ010000002.1  80408024-80408215   c
            1654-1785           JAXUCZ010000002.1  80399808-80399939   c
            1786-2016           JAXUCZ010000002.1  80391850-80392080   c
            2017-2139           JAXUCZ010000002.1  80383975-80384097   c
            2140-2331           JAXUCZ010000002.1  80373308-80373499   c
            2332-2501           JAXUCZ010000002.1  80358945-80359114   c
            2502-2676           JAXUCZ010000002.1  80357780-80357954   c
            2677-2872           JAXUCZ010000002.1  80354222-80354417   c
            2873-3039           JAXUCZ010000002.1  80353426-80353592   c
            3040-3159           JAXUCZ010000002.1  80351555-80351674   c
            3160-3351           JAXUCZ010000002.1  80349910-80350101   c
            3352-3501           JAXUCZ010000002.1  80349344-80349493   c
            3502-3616           JAXUCZ010000002.1  80347525-80347639   c
            3617-3732           JAXUCZ010000002.1  80344538-80344653   c
            3733-3855           JAXUCZ010000002.1  80341105-80341227   c
            3856-4050           JAXUCZ010000002.1  80335508-80335702   c
            4051-4166           JAXUCZ010000002.1  80335296-80335411   c
            4167-4233           JAXUCZ010000002.1  80334547-80334613   c
            4234-4343           JAXUCZ010000002.1  80333736-80333845   c
            4344-4413           JAXUCZ010000002.1  80333085-80333154   c
            4414-4503           JAXUCZ010000002.1  80332518-80332607   c
            4504-4596           JAXUCZ010000002.1  80330691-80330783   c
            4597-4708           JAXUCZ010000002.1  80328349-80328460   c
            4709-4899           JAXUCZ010000002.1  80326800-80326990   c
            4900-5001           JAXUCZ010000002.1  80324976-80325077   c
            5002-5144           JAXUCZ010000002.1  80322476-80322618   c
            5145-5244           JAXUCZ010000002.1  80321665-80321764   c
            5245-5488           JAXUCZ010000002.1  80309747-80309990   c
            5489-5781           JAXUCZ010000002.1  80278009-80278301   c
            5782-5952           JAXUCZ010000002.1  80277057-80277227   c
            5953-6048           JAXUCZ010000002.1  80274471-80274566   c
            6049-6197           JAXUCZ010000002.1  80271721-80271869   c
            6198-6264           JAXUCZ010000002.1  80268470-80268536   c
            6265-6368           JAXUCZ010000002.1  80266833-80266936   c
            6369-6438           JAXUCZ010000002.1  80261547-80261616   c
            6439-6528           JAXUCZ010000002.1  80259668-80259757   c
            6529-6621           JAXUCZ010000002.1  80258444-80258536   c
            6622-6672           JAXUCZ010000002.1  80257326-80257376   c
            6673-6750           JAXUCZ010000002.1  80257019-80257096   c
            6751-6942           JAXUCZ010000002.1  80255974-80256165   c
            6943-7120           JAXUCZ010000002.1  80253244-80253421   c
            7121-7929           JAXUCZ010000002.1  80251304-80252112   c
            7930-8177           JAXUCZ010000002.1  80247423-80247670   c
            8178-8316           JAXUCZ010000002.1  80245874-80246012   c
            8317-8344           JAXUCZ010000002.1  80245369-80245396   c
            8345-8507           JAXUCZ010000002.1  80245018-80245180   c
            8508-8629           JAXUCZ010000002.1  80244676-80244797   c
            8630-8708           JAXUCZ010000002.1  80241174-80241252   c
            8709-8909           JAXUCZ010000002.1  80240130-80240330   c
            8910-9048           JAXUCZ010000002.1  80238306-80238444   c
            9049-11401          JAXUCZ010000002.1  80235485-80237837   c
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q22"
     gene            1..11401
                     /gene="Trio"
                     /note="trio Rho guanine nucleotide exchange factor"
                     /db_xref="GeneID:310192"
                     /db_xref="RGD:1308360"
     exon            1..442
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..9597
                     /gene="Trio"
                     /EC_number="2.7.11.1"
                     /note="triple functional domain (PTPRF interacting);
                     PTPRF-interacting protein"
                     /codon_start=1
                     /product="triple functional domain protein"
                     /protein_id="NP_001101128.3"
                     /db_xref="GeneID:310192"
                     /db_xref="RGD:1308360"
                     /translation="MSGSSGGATAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLA
                     DIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRMRQE
                     DLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHIALIIK
                     PDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRV
                     AFEDYISNAAHMLSRLEELQDVLAKKELPQDLEGARNMIDEHSQLKKKVIKAPIEDLD
                     LEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKL
                     KLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGASHPHAMELQTQHNHFAMNCM
                     NVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIF
                     HQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSL
                     LDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRL
                     QLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNA
                     DKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKE
                     LWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRD
                     SAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDI
                     ISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYV
                     NEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQA
                     EVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAE
                     AMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESL
                     EQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYL
                     HRNSVSMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERS
                     AKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLAD
                     GFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLD
                     IIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLW
                     EMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWAD
                     KFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLL
                     KELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQV
                     WDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDP
                     CKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTVHLRGALKEPIHIPKT
                     APAARQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFT
                     ACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSME
                     MEGIFNHKDSLSVSSNDASPPASVASLQPHMMGAQSSPGPKRPGNTLRKWLTSPVRRL
                     SSGKADGHAKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETIEERGRNEGLSSG
                     TLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTS
                     SETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDE
                     MEERKCSSLKRRHYVLQELVETERDYVRDLGCVVEGYMALMKEDGVPDDMKGKDKIVF
                     GNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSE
                     YIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAV
                     EVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFL
                     FEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVESDPCKFALTSRTGDAVET
                     FILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSSGGGG
                     GSGGSGASSGGSSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPMLVSSAASSQAEA
                     DKMSGMSAPSPSLPTPSNSLALEASLGQPSRLPLSGDSEGHERETEPIPKMKVMESPR
                     KALGSTSGTSQDGNTKDARGNLGPLPLGKTRPGAVSPLNSPLSTTFPSPFGKEAFPPS
                     SPLQKGGSFWSSIPASPASRPSSFTFPGDSDSLQRQTHRHAAPSKDTDRMSTCSSASE
                     QSVQSTQSNGSESSSSSNISTMLVTHEYTAVKEDEINVYQGEVVQILASNQQNMFLVF
                     RAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTFKKSTSWHTALRLRKKSEKKDKDGK
                     RDGKLENGYRKPREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVFRCRV
                     CGRPKASITWKGPEHNTLNNDDHYNISYSDVGEATLKIIGVSTEDDGVYTCIAVNDMG
                     SASSSASLRVLGPGSDGIVVTWKDNFDAFYSEVAELGRGRFAVVKKCDQKGTKRAVAT
                     KFVNKKLMKRDQVTHELGILQNLQHPLFVSLLDTFETPTSYVLVLELADQGRLLDCVV
                     RWGSLTEGKVRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDQSLAKPTIKLTDFGDA
                     VQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTADTWSVGVLTYVLLSGVSPFLDDSVE
                     ETCLNICRLDFSFPEDYFQGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGS
                     GKGMGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV"
     exon            443..517
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            518..632
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            633..825
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1338
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1461
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1653
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1785
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..2016
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2139
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2331
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2501
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2676
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2677..2872
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..3039
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3040..3159
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3351
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3501
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3502..3616
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3617..3732
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3855
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4050
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4051..4166
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4233
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4234..4343
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4344..4413
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4503
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4504..4596
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4597..4708
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4709..4899
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            4900..5001
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5002..5144
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5145..5244
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..5488
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5489..5781
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5782..5952
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            5953..6048
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6049..6197
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6198..6264
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6265..6368
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6369..6438
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6439..6528
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6529..6621
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6622..6672
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6673..6750
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6751..6942
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            6943..7120
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            7121..7929
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            7930..8177
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8178..8316
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8317..8344
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8345..8507
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8508..8629
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8630..8708
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8709..8909
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            8910..9048
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
     exon            9049..11401
                     /gene="Trio"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccgccctcg cgtgtccgcg ccgctgcccg cgccccggcc ggccacacag aggccgtggc
       61 tccgcgcgcc cgccccgcgc caggctcgcg gttcccgggc ggtgtcctcg tctatgtggg
      121 cgcgctcctc gggccgagcc gccgccgccc gccgccccgg ggctgtgcgt ccgcgcgccg
      181 ggcgcgggca gctgggtgtt cggcgccgtc aggccaggcg cgggcggctg ggtgcgcgga
      241 gctaggggcg cggggcctga ggcgggctcg tccgccgccg aagccatgag cggcagcagc
      301 ggcggtgcca ccgccccggc cgcgtcctcc ggcccggcgg cggcggccag cgcggcgggc
      361 tccggctgcg ggggcggcgc cggcgagggt gccgaggagg ccgccaagga cctggccgac
      421 atcgcggcct tctttcgatc cgggtttcga aaaaatgatg aaatgaaagc tatggatgtt
      481 ttgcccatcc tgaaggaaaa agtcgcctac ctttcaggtg gcagagataa gcgtggaggt
      541 cccatattaa cgtttccagc ccgcagcaat catgatagaa tgcggcagga ggacctcagg
      601 aggctcatct cctatttagc ctgcattccc agcgaggaag tctgcaagcg agggttcacg
      661 gtgattgtgg acatgcgcgg ctccaagtgg gactccatca aaccactgtt gaagatcctg
      721 caggagtcct tcccctgctg catccacatc gccctgataa tcaagcccga caacttctgg
      781 cagaaacaga ggacgaattt tggcagttcc aaatttgaat ttgagacaaa tatggtctct
      841 ttagaaggcc ttaccaaagt ggttgacccc tcccagctaa cccctgagtt tgatggctgc
      901 ctagagtaca accatgagga atggatagaa atccgagtag cttttgagga ctacattagc
      961 aatgcagccc acatgctgtc ccggctagag gaacttcagg acgtccttgc aaaaaaggag
     1021 ttacctcagg acctagaggg ggctcggaac atgattgacg agcattctca gctgaagaag
     1081 aaggtgatca aggctcccat cgaggacttg gacttagagg ggcagaagct gcttcagcgg
     1141 atccagagca gcgagagttt tcccaaaaag aactcaggct cgggcaatgc agacctgcag
     1201 aacctcttgc ccaaggtgtc caccatgctt gacaggctgc actcgactcg gcagcacctg
     1261 caccagatgt ggcacgtgag gaaactgaag ttggaccagt gcttccagct gcggctgttc
     1321 gaacaggatg ctgagaagat gtttgactgg atcacacaca acaaaggcct gtttctaaac
     1381 agctacacgg agatcggggc cagccaccct cacgccatgg agcttcagac gcagcacaat
     1441 cactttgcta tgaactgtat gaacgtgtat gtaaatatca accgcataat gtcggtggcc
     1501 aatcgcctgg tggagtcggg ccactatgcc tctcagcaga ttaagcagat cgccaatcag
     1561 ctggaacagg agtggaaagc gttcgcagcg gccttggacg agcgcagcac cttgttggac
     1621 atgtcctcca tctttcatca gaaggctgag aagtacatga gcaatgtgga ctcgtggtgt
     1681 aaggcctgtg gtgaagtaga ccttccctcg gagctgcagg acctggaaga tgccattcat
     1741 catcaccagg ggatctatga gcacatcacc ctcgcttact ctgaggtaag ccaggatggg
     1801 aagtctctcc ttgataagct ccagcgcccc ctgacccccg gcagctctga ttccctcacg
     1861 gcctctgcca actactccaa ggcggtgcac catgtcttgg atgtcatcca cgaggtcctg
     1921 caccaccagc gccagctcga gaacatctgg cagcaccgca aggttcggct ccaccagagg
     1981 ctgcagctct gtgtcttcca gcaggacgtt cagcaggtgc tggactggat agagaaccac
     2041 ggagaagcat ttctgagcaa acacacaggc gtgggcaaat cccttcaccg ggccagagct
     2101 ttgcagaaac gccatgagga cttcgaggaa gttgcacaga acacgtacac aaatgcagat
     2161 aaactgctag aagcagcaga gcagctggct cagactgggg aatgtgaccc cgaagagatt
     2221 tatcaggctg cccatcagct tgaagaccgg atacaagact ttgttcggcg agttgaacag
     2281 cggaagatcc tgctggacat gtctgtgtca tttcacaccc atgtaaaaga gctctggaca
     2341 tggctggagg agctgcagaa ggaactgctg gacgacgtgt acgccgagtc ggtggaggcc
     2401 gtccaggatc tcatcaagag attcggccag cagcagcaga ccaccctgca ggtgacagtc
     2461 aacgtgatca aggaagggga ggacctcatc cagcagctga gggactctgc catctccagt
     2521 aacaagacgc cgcacaacag ctccatcaac cacatcgaga cggtgctgca gcagctggac
     2581 gaggctcagt cccagatgga ggagctcttc caggaacgca agatcaagct ggaactcttc
     2641 ctgcagctac gcatattcga gcgggatgcc atcgacatca tctcggacct tgagtcttgg
     2701 aatgatgaac tttcccagca aatgaacgac ttcgatacag aggatctgac gatcgctgag
     2761 cagcgcctgc agcaccatgc ggacaaagcc ctaaccatga ataacttgac tttcgacgtc
     2821 atccaccagg gacaggatct tctgcagtac gtcaatgagg tccaggcctc tggtgtggag
     2881 ctgctttgcg acagagatgt ggacatggcc actcgggttc aggacctcct ggagtttctt
     2941 cacgaaaagc agcaggagtt ggatttagcg gccgagcagc atcggaaaca cttggagcag
     3001 tgtgtgcagt tgcgccacct gcaggcagaa gtgaagcagg tactgggctg gatccggaat
     3061 ggagagtcca tgctgaatgc tgggctcatc acggccagct cgctgcagga ggccgagcag
     3121 ctgcagagag agcacgagca gttccagcac gctattgaga aaacacacca gagtgcactg
     3181 caggtgcagc agaaggcgga ggccatgctg caggccaacc actacgacat ggacatgatc
     3241 cgtgactgtg cagagaaggt cgcctcgcac tggcagcagc tcatgctcaa gatggaagac
     3301 cgcctcaagc tcgtcaacgc ctccgtcgcc ttctacaaga cttctgagca ggtctgcagt
     3361 gttctggaga gcctggagca ggagtacaag agagaggagg actggtgtgg tggagccgac
     3421 aagctgggcc ccaattctga gactgaccac gtcaccccaa tgattagcaa gcacctcgaa
     3481 cagaaggaag ccttcctgaa ggcttgcaca ctggccagga ggaacgcaga tgtcttccta
     3541 aaatacctac acaggaacag cgtgagcatg ccggggatgg tgacacacat caaagctcca
     3601 gaacagcaag taaaaaatat cttgaacgaa ctcttccagc gggaaaacag ggtattgcat
     3661 tattggacca tgagaaaaag gcggctggac cagtgccagc agtacgtggt ctttgagagg
     3721 agtgccaagc aggcattaga atggatccac gacaatgggg agttctatct ttccacacat
     3781 acctcaacag ggtccagcat tcagcataca caggagctgt tgaaagaaca tgaagagttt
     3841 cagataactg ccaagcaaac caaagagagg gtgaagctat tgatacagct ggctgatggc
     3901 ttttgtgaaa aagggcatgc ccatgcagca gaaattaaaa aatgtgtgac cgcagtggat
     3961 aagaggtaca gagatttctc tctgcgcatg gagaagtacc ggacctcttt ggagaaagcc
     4021 ctggggattt cttcagattc caacaaatcg agtaaaagcc tgcagttgga catcatccct
     4081 gccagtatcc ccgggtcaga ggtgaagctt cgggatgctg ctcacgaact caatgaagag
     4141 aagcgcaagt ctgcccgcag gaaagagttc ataatggctg agttaattca aaccgaaaag
     4201 gcttatgtaa gagaccttcg agaatgtatg gatacctacc tgtgggaaat gaccagcgga
     4261 gtggaggaga tcccgcccgg catcgtcaac aaggagctca tcatcttcgg gaacatgcag
     4321 gagatctacg agttccataa caacatcttc ctgaaggagc tggagaagta cgagcagctc
     4381 ccagaggacg tggggcactg ctttgtcacc tgggcagaca agtttcagat gtatgtcaca
     4441 tactgcaaaa ataaacctga ttctacgcag ctgatcctgg aacacgcagg gtcctacttt
     4501 gacgagatcc agcagcggca tgggttagcc aattccatct cttcctacct gatcaaacca
     4561 gttcagcgaa taacaaagta ccagctcctt ttaaaagagc tgctcacatg ctgtgaagaa
     4621 ggaaaggggg agattaaaga tggcctcgag gtgatgctca gtgtgcccaa gcgagccaat
     4681 gatgccatgc acctcagcat gctggaaggg tttgatgaaa acattgagtc tcagggagag
     4741 ctcatcctgc aggagtcctt ccaagtgtgg gacccaaaaa ccttaattcg gaagggtcga
     4801 gaacgccatc tcttcctttt tgaaatgtcc ttagtgttca gtaaagaagt gaaagactcc
     4861 agcgggagaa gcaagtacct gtacaaaagc aagctgttta cctcagagtt gggtgtcacg
     4921 gaacatgttg aaggagatcc ttgcaaattt gcactgtggg tggggaggac accaacttca
     4981 gataataaaa ttgtccttaa ggcctccagt atcgagaaca aacaggactg gataaagcac
     5041 atccgtgagg tgatacagga gaggacggtg cacctgagag gagccctgaa ggagcccatc
     5101 cacatcccca agacggctcc cgcagcaagg cagaagggga ggagggatgg agaggacctg
     5161 gacagccaag gggacggcag cagccagccc gacaccattt ccattgcctc ccggacatca
     5221 cagaacacac tggacagtga caagctttct ggcggctgcg agctgacagt ggtgatccat
     5281 gacttcacag cttgcaacag caatgagctg accatccggc gaggccagac agtggaggtt
     5341 ctcgaacggc ctcacgacaa gcccgactgg tgtctggtgc gcaccactga taggtcccct
     5401 gcagctgaag gcctcgtgcc ctgtggctca ctgtgcattg cccactcacg aagtagcatg
     5461 gagatggagg gcatcttcaa ccacaaagac tcactgtccg tctccagtaa tgacgccagc
     5521 ccaccggcct ctgtggcctc ccttcaaccc cacatgatgg gagcacagag ttcaccaggt
     5581 cccaagcggc cgggcaacac cctgcgcaag tggctcacaa gccccgtacg gcggctcagc
     5641 agtggcaagg ccgatggaca tgcgaagaag ctggcccaca agcacaagaa gagcagggag
     5701 gtccggaaga gtgcggacgc tggatcacag aaggattcag atgacagcgc tgccacccca
     5761 caggatgaga ccatcgaaga gagaggacgg aacgagggcc tcagcagtgg tacgctgtcc
     5821 aaatcctcgt cctcgggcat gcagagctgc ggagaagaag aaggagagga gggcgccgac
     5881 gctgtaccac tgcctccgcc catggccatc cagcagcaca gcctccttca gccagactcg
     5941 caggatgaca aggcctcttc gaggttatta gtccgcccca ccagctctga gactcccagt
     6001 gcggctgagc tcgtcagtgc gattgaagaa ctcgtgaaga gcaaaatggc gctggaggac
     6061 cgccctagct cactgcttgt tgaccaggga gacagtagca gcccgtcctt caatccctca
     6121 gacaattctc tcctctcatc ctcctcgccc attgacgaga tggaagaaag aaaatgcagc
     6181 tctctaaaga gaagacacta tgttttgcaa gaattagtgg agacagagcg agactatgtg
     6241 cgggaccttg gatgtgtggt tgagggctac atggcgctga tgaaggaaga cggcgttcct
     6301 gatgacatga aggggaaaga caagatcgtg tttggcaaca tccatcagat ttacgactgg
     6361 cacagagact tttttttagg agagttggag aagtgccttg aagatccaga aaaactagga
     6421 tccctttttg taaaacacga gagaaggttg cacatgtaca tagtttattg tcaaaataaa
     6481 ccaaagtctg agcacattgt ctccgagtac atcgacacct tttttgagga cttaaagcag
     6541 cgcctgggcc acagactgca gctcactgat ctgttgatca aacctgtgca gagaatcatg
     6601 aagtaccagc tgctcctgaa ggacttcctc aagtactcaa aaaaagctag cctggacacg
     6661 tccgaattag agaaagctgt agaggtcatg tgcatagtgc cgaagcgctg caacgacatg
     6721 atgaacgtcg ggcggctgca aggatttgac ggtaaaattg ttgcccaggg taaactgctc
     6781 ttacaggaca cattcctggt cacagaccaa gatgcagggc ttctgccccg atgcaaagag
     6841 aggcgggttt tcctctttga acagattgtc atattcagcg aaccgcttga taagaaaaag
     6901 ggcttttcca tgccaggatt tctgttcaag aacagtatca aggtgagttg cctttgcctg
     6961 gaggagaatg tggagagcga tccctgtaag tttgccctga catccaggac gggcgatgcc
     7021 gtggagacct tcattttgca ttcatccagt ccaagtgtcc gacaaacgtg gatccatgaa
     7081 atcaaccaaa tcttagaaaa ccagcgcaac tttttaaatg ccttgacatc acccatcgag
     7141 taccagagga accatagcgg gggcggcggc agtggcagcg ggggcagcag cggcgggggc
     7201 gggggcagcg gtggcagcgg ggcctccagt ggaggcagca gcagccatgg cagtggcccc
     7261 agcagctgca gcagtggccc cagctcgagc aggagcaggc cctccaggat cccacagcct
     7321 gtcagacacc acccccccat gctggtctcc tctgcagcct caagccaggc agaggcagac
     7381 aagatgtcag gtatgtctgc tcccagcccc tcactgccta cccccagcaa cagcctggcc
     7441 ctcgaggcca gccttggcca gcccagcagg ctccccctga gtggagactc cgaaggtcat
     7501 gagcgagaga cagaacccat tcccaagatg aaggtgatgg agagcccccg gaaggccctg
     7561 gggagtactt ccggtacaag ccaagatgga aacaccaagg atgcccgggg gaacctgggc
     7621 ccgctgcctc tgggcaagac taggccgggg gccgtctcgc cgctgaactc tcctctctcc
     7681 accacgttcc cttctccttt tggcaaggag gcctttcctc ccagcagccc cctgcagaag
     7741 gggggctcct tctggagctc cattcctgct tcccctgcca gccggccaag ctccttcacc
     7801 ttcccggggg acagtgactc cctccagcga cagacgcacc gccatgcagc ccccagcaag
     7861 gacacggacc gcatgagcac gtgctcctcg gccagcgagc agtccgtgca gtccactcag
     7921 agcaacggga gtgaaagcag cagcagtagc aacatctcca ccatgctggt gacacacgag
     7981 tacacggcag tgaaggagga tgagataaac gtctatcaag gagaggtcgt tcaaattctg
     8041 gccagcaacc agcagaacat gtttctggtg ttccgagccg ccactgacca gtgccccgca
     8101 gccgagggtt ggattccagg cttcgtcttg ggccatacga gtgcagttat catggagaac
     8161 ccggacggga ctttcaagaa gtcaacatct tggcacacag cactccgttt aaggaagaag
     8221 tctgagaaga aagataaaga cggcaaaagg gacggcaagt tggagaacgg gtacaggaag
     8281 ccacgagaag ggctcagtaa caaggtatct gtgaagcttc tcaaccccaa ctatatctat
     8341 gacgttcccc cagaattcgt tatcccattg agcgaggtca catgtgagac aggggagacc
     8401 gttgttttta gatgtcgtgt ctgtggccgc cccaaggcct caatcacctg gaagggccct
     8461 gaacacaaca ctttgaacaa cgatgaccac tacaacatct cctacagtga cgttggggag
     8521 gccactctga agatcattgg tgtgagcaca gaagatgatg gtgtctacac ctgcatcgct
     8581 gtgaacgaca tgggttcagc ctcgtcttct gccagtctga gggttctagg tccagggagt
     8641 gatgggatcg tggtgacctg gaaggacaac ttcgacgcct tctacagtga ggtggctgag
     8701 ctcggcaggg gcagatttgc tgtagttaag aaatgtgacc agaagggaac caaacgagca
     8761 gtggccacaa agtttgtgaa caagaagttg atgaagcgtg accaggtcac tcatgagctt
     8821 ggcatcctgc agaacctcca gcaccccctc ttcgtgagcc ttctagacac cttcgagacc
     8881 cccaccagtt acgtcctggt tttggaattg gctgaccaag gtcgcctcct agactgtgtg
     8941 gtacgatggg gaagtctcac cgaagggaag gtcagagcgc acctggggga agttctggaa
     9001 gccgtccgat accttcacaa ctgcaggata gcacacctgg acctaaagcc tgagaacatc
     9061 ttggtggatc agagtttggc caagccaacc attaaactca ctgactttgg agacgctgtc
     9121 cagctcaaca ccacctacta catccaccag ttgctgggga accccgagtt tgcagccccg
     9181 gaaatcattc ttgggaaccc tgtctccctg accgcggaca cgtggagcgt tggggtgctc
     9241 acgtacgtgc tgctcagcgg cgtgtccccc ttcttggatg acagcgtgga agagacctgc
     9301 ctgaacattt gccgactgga cttcagcttc ccagaggact actttcaagg agttagccag
     9361 aaggccaagg aattcgtgtg cttcctcctg caggaggacc ccgccaagcg tccctcggct
     9421 gcactggccc tacaggagca gtggctgcag gcaggtaacg gcagcggcaa aggcatgggc
     9481 gtcctcgaca cgtccagact gacctccttc attgagcggc gcaaacacca gaatgatgtt
     9541 cgacctatcc gtagcattaa aaacttctta cagagcaggc ttctgcctag agtttgacct
     9601 atcttgaagt tctttctcat tctctttcac ctgccaatca gctgttaatt tgaattttga
     9661 agaggaaacc aaaccaaact aactggccgg ccgccggccg ttcatcgtgt gaaatggcat
     9721 tccaagtgag ctgtgctcgg ccgcgctcgg gctcagagct gcacgctgca ctggggtgga
     9781 ggaccttcag ttacgctctg cacaggcaga gatcacattg ctgtaccaca gtatcttatt
     9841 caggttcctg caaaaaaata aataaataaa gagatacttt tttaaacgaa catggataga
     9901 attttgcaaa tttaacattt tcaagattta ttcaaggaca cctatgccta tgttcagacc
     9961 actggtgtta caccaaactg agatactgtg catttctagg aaagacgagt caggtggtag
    10021 ccactcccat ggcctcggcc agtgctaggc gtccacgtgg cactgagctt ttccaacggc
    10081 ctcgtctgga tacaacttgc cgggcaccag acttggcttc tgaaacgtgc attcaacctc
    10141 aatcttttgc ataaaatgta atgaatggtt ttgctctgat gaaatgtagg ccttacttgt
    10201 acataagact cttcctgcct tttgtctggc gtcccccatc ccttcccctc ctacccacca
    10261 cctgggcctt cctctcgggg gtctgatggg gtctgcccat ccaatggaga ctttaggctg
    10321 ggtccagtcc cactgccccc tccctagccc caccccaagc cgtcaatcag attgttgaac
    10381 ggtatacagt cagatgaaaa tactgtaaat gcactcattg gggttttttt tttttttttg
    10441 gttttttgtt tgttttgttt tttttttaat ttcataccat gtgcaatgtt gtgactttaa
    10501 cattttctgc aactatttat gaagacttct gttgtacctg taataaatat atagaaaaag
    10561 cacattcttc gcaagtgagc tttatggttc tgtgtgtgtt gggggtgggt cgtagccctg
    10621 tgccatcagt caaggatact taactcttcg tgaaatttgt cagctttgag gactgtaaaa
    10681 agtgatttca tactctgaat ataaaactgg ataatagggt aatgttttaa attttattat
    10741 gctattattc agaatgccaa agtattattt ttttcccaga aatcagtctg aacatttact
    10801 actttttaga ctttttgaca ttcaactttc tgtataaaaa taattggctg gattttaagc
    10861 ttttggtaag aaagccaatc aagtactggc cgccccgagg ctggtgcaca gtgcccgagt
    10921 cacgtggcag agctggctct ggtgcgggaa gtccttcacc acagaagttg gatttctggc
    10981 acacttggat ttttctggat gtctttttac ctttattgtg tgaaatacac taaaaaagag
    11041 accggcttgc ttcccaaggc agcgagacat ctgggtcgtg agccataaac gggcataact
    11101 cagctttgca gtttccaacg tattttattt attcttctgt tgggtttttg tttgtttctt
    11161 tgtaaaattg tacagaaact ttttaagaaa aattggattc aaaaactgga tgtgtatctg
    11221 taacctcata acttttattt gtgtaccgtc cctttgatta tttcagttaa aagtttttac
    11281 attatttttt ttgcatgtat atttctttgt acagagacct tacatgttta cacagtatga
    11341 tgtgatgtaa atattttatt ttgccatcag ttattttaag aaattaaaca tatttgcctg
    11401 a
//
