LOCUS       NM_001108991            6477 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Rattus norvegicus thyrotropin-releasing hormone degrading enzyme
            (Trhde), mRNA.
ACCESSION   NM_001108991 XM_001078601 XM_039079683 XM_345824
VERSION     NM_001108991.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Lazcano,I., Cabral,A., Uribe,R.M., Jaimes-Hoy,L., Perello,M.,
            Joseph-Bravo,P., Sanchez-Jaramillo,E. and Charli,J.L.
  TITLE     Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of
            the Mediobasal Hypothalamus of Male Adult Rats
  JOURNAL   Endocrinology 156 (7), 2713-2723 (2015)
   PUBMED   25942072
  REMARK    GeneRIF: Delayed increases in both the median eminence PPII as well
            as the thyroliberinase activities in fasted male rats may
            facilitate the maintenance of deep downregulation of the HPT axis
            function, despite a partial reactivation of TRH expression in the
            PVN.
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Alvarez-Salas,E., Aceves,C., Anguiano,B., Uribe,R.M.,
            Garcia-Luna,C., Sanchez,E. and de Gortari,P.
  TITLE     Food-restricted and dehydrated-induced anorexic rats present
            differential TRH expression in anterior and caudal PVN. Role of
            type 2 deiodinase and pyroglutamyl aminopeptidase II
  JOURNAL   Endocrinology 153 (8), 4067-4076 (2012)
   PUBMED   22719053
  REMARK    GeneRIF: Food-restricted and dehydrated-induced anorexic rats
            present differential TRH expression in anterior and caudal PVN.
            Role of type 2 deiodinase and pyroglutamyl aminopeptidase II.
REFERENCE   3  (bases 1 to 6477)
  AUTHORS   Lazcano,I., Uribe,R.M., Martinez-Chavez,E., Vargas,M.A.,
            Matziari,M., Joseph-Bravo,P. and Charli,J.L.
  TITLE     Pyroglutamyl peptidase II inhibition enhances the analeptic effect
            of thyrotropin-releasing hormone in the rat medial septum
  JOURNAL   J Pharmacol Exp Ther 342 (1), 222-231 (2012)
   PUBMED   22532627
  REMARK    GeneRIF: Twenty to 35% of Pyroglutamyl peptidase II mRNA-labeled
            profiles were positive for TRH-receptor 1 (TRH-R1) mRNA.
REFERENCE   4  (bases 1 to 6477)
  AUTHORS   Konishi,H., Ogawa,T., Kawahara,S., Matsumoto,S. and Kiyama,H.
  TITLE     Continuous stress-induced dopamine dysregulation augments PAP-I and
            PAP-II expression in melanotrophs of the pituitary gland
  JOURNAL   Biochem Biophys Res Commun 407 (1), 7-12 (2011)
   PUBMED   21329657
  REMARK    GeneRIF: PAP-I and PAP-II expression was induced by the decreased
            dopamine levels in the intermediate lobe of the pituitary under
            continuous stress.
REFERENCE   5  (bases 1 to 6477)
  AUTHORS   Sanchez,E., Vargas,M.A., Singru,P.S., Pascual,I., Romero,F.,
            Fekete,C., Charli,J.L. and Lechan,R.M.
  TITLE     Tanycyte pyroglutamyl peptidase II contributes to regulation of the
            hypothalamic-pituitary-thyroid axis through glial-axonal
            associations in the median eminence
  JOURNAL   Endocrinology 150 (5), 2283-2291 (2009)
   PUBMED   19179432
  REMARK    GeneRIF: An increase in circulating thyroid hormone up-regulates
            PPII activity in tanycytes and enhances degradation of
            extracellular TRH in the median eminence through glial-axonal
            associations.
REFERENCE   6  (bases 1 to 6477)
  AUTHORS   Chavez-Gutierrez,L., Matta-Camacho,E., Osuna,J., Horjales,E.,
            Joseph-Bravo,P., Maigret,B. and Charli,J.L.
  TITLE     Homology modeling and site-directed mutagenesis of pyroglutamyl
            peptidase II. Insights into omega-versus aminopeptidase specificity
            in the M1 family
  JOURNAL   J Biol Chem 281 (27), 18581-18590 (2006)
   PUBMED   16611635
  REMARK    GeneRIF: shortening of side chain at PPII-269 position is required
            for adjustment to thepyroglutamyl residue
REFERENCE   7  (bases 1 to 6477)
  AUTHORS   Schmitmeier,S., Thole,H., Bader,A. and Bauer,K.
  TITLE     Purification and characterization of the thyrotropin-releasing
            hormone (TRH)-degrading serum enzyme and its identification as a
            product of liver origin
  JOURNAL   Eur J Biochem 269 (4), 1278-1286 (2002)
   PUBMED   11856362
REFERENCE   8  (bases 1 to 6477)
  AUTHORS   Papadopoulos,T., Heuer,H. and Bauer,K.
  TITLE     Analysis of the thyrotropin-releasing hormone-degrading ectoenzyme
            by site-directed mutagenesis of cysteine residues. Cys68 is
            involved in disulfide-linked dimerization
  JOURNAL   Eur J Biochem 267 (9), 2617-2623 (2000)
   PUBMED   10785382
REFERENCE   9  (bases 1 to 6477)
  AUTHORS   Schauder,B., Schomburg,L., Kohrle,J. and Bauer,K.
  TITLE     Cloning of a cDNA encoding an ectoenzyme that degrades
            thyrotropin-releasing hormone
  JOURNAL   Proc Natl Acad Sci U S A 91 (20), 9534-9538 (1994)
   PUBMED   7937801
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On or before Jul 20, 2021 this sequence version replaced
            XM_039079683.1, NM_001108991.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1733              JAXUCZ010000007.1  52235111-52236843   c
            1734-2007           JAXUCZ010000007.1  52222421-52222694   c
            2008-2134           JAXUCZ010000007.1  52125077-52125203   c
            2135-2289           JAXUCZ010000007.1  52013491-52013645   c
            2290-2403           JAXUCZ010000007.1  52009848-52009961   c
            2404-2541           JAXUCZ010000007.1  51992138-51992275   c
            2542-2607           JAXUCZ010000007.1  51953439-51953504   c
            2608-2673           JAXUCZ010000007.1  51935390-51935455   c
            2674-2861           JAXUCZ010000007.1  51934228-51934415   c
            2862-2950           JAXUCZ010000007.1  51930402-51930490   c
            2951-3084           JAXUCZ010000007.1  51920335-51920468   c
            3085-3140           JAXUCZ010000007.1  51920168-51920223   c
            3141-3288           JAXUCZ010000007.1  51877378-51877525   c
            3289-3386           JAXUCZ010000007.1  51875279-51875376   c
            3387-3494           JAXUCZ010000007.1  51873752-51873859   c
            3495-3662           JAXUCZ010000007.1  51846536-51846703   c
            3663-3803           JAXUCZ010000007.1  51845866-51846006   c
            3804-3885           JAXUCZ010000007.1  51842234-51842315   c
            3886-6477           JAXUCZ010000007.1  51832111-51834702   c
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..6477
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /note="thyrotropin-releasing hormone degrading enzyme"
                     /db_xref="GeneID:366894"
                     /db_xref="RGD:728895"
     exon            1..1733
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             829..4029
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /EC_number="3.4.19.6"
                     /note="TRH-specific aminopeptidase; thyroliberinase;
                     pyroglutamyl-peptidase II; TRH-DE; TRH-degrading
                     ectoenzyme"
                     /codon_start=1
                     /product="thyrotropin-releasing hormone-degrading
                     ectoenzyme"
                     /protein_id="NP_001102461.1"
                     /db_xref="GeneID:366894"
                     /db_xref="RGD:728895"
                     /translation="MALDGERGEQEEEKKKKKKKKKRKKKEEEGAEKSSSPFAATMGE
                     DDAALRASGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSIVALLAVTMLAVLL
                     SLRFDECGASAAMPGTDGGLGGFPERGGNSSYPGSARRNHHAGEESSQREIGEVGTAG
                     TPSAHPPSEEEQEQWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACQNA
                     TRYVVLHASRVAVEKVQVAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRHYNLK
                     IIYNALIENELLGFFRSSYVIHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISI
                     KHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGV
                     VVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMEN
                     WGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFA
                     HYFEFVGTDYLYPSWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLRATDIDKVFDW
                     IAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYV
                     NIQEVMDQWTLQMGYPVITILGNMTAENRILITQQHFIYDIGAKTKALQLQNSSYLWQ
                     IPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWILGNINQTGYFRVNYDLRNW
                     RLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHA
                     ASRALYPLDKLLDRMENYNIFNEYILKQVATTYSKLGWPKNNFNGSVVQASYQHEELR
                     REVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFI
                     WMKFHSTTAVSEKKILLEALTCSDDRNLLSRLLNLSLNSEVVLDQDAIDVIIHVARNP
                     HGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKSYDGV
                     ASASFSRAVETVEANVRWKRLYQDELFQWLGKAMRH"
     exon            1734..2007
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2134
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2289
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2403
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2541
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2607
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2673
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2861
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2862..2950
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3084
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3140
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3288
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3289..3386
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3494
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3662
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3803
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3885
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3886..6477
                     /gene="Trhde"
                     /gene_synonym="PAP-II"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgaagtgga cgcccgggcc ggacacttct tcacttgtct tctctggagg gcagagcaat
       61 atcggaacgc ggaaggcttg ctgcccagtc cgcaacctca cgccttttac cgccaccact
      121 gcttaaagtg tccagtgtgg agaaggcttc ttcaggaccg atgctccgcg gccgtgggac
      181 ttcctccggc tagcttactc ccgtcctttc cttggtcctc tgctccatcc tctgctctcc
      241 cacagcgagg tggaaggcac cagcgccttc cgggctgccg ccgccgccgc aactgttttt
      301 gcgaccacat cccacccaga agaccgaaag ctgctggagt gcggggagct gggtgaagcc
      361 ctaacttgcc cccctgttca gtcgctctcc ccagcgctct ctgtcctcct ggccgagccg
      421 ctgagagagc tgccgccgct agcctggaag agtgcggcgc ccgggaggac acgcgagccg
      481 caggcgcaca ggggcggggg cggcgtgtgc ccgggcgccc ggtgctagcc cgacgagtag
      541 cgcgcacccg gcgagcgaca cgctccccaa gttgggcgcg tcccagagct ggttgccccg
      601 ctatccgcag ctggtgtctg gagagaggcg gggctgatgg gggtcgtctc ggtcgtcact
      661 agcctccaga gtctgtctta agagaatctg agctggcctt cctaaggtcc cagccggcgt
      721 cgcgccctcc gcccgcgtgc ggtctctgat gactgcgctg gaggtggccc gggtggcccg
      781 cccgctaggg gtgtcggagg gggcggggga agaggaggag gcggtgtgat ggccctggac
      841 ggcgagaggg gggagcaaga ggaggagaag aaaaagaaga agaagaaaaa gaagaggaag
      901 aagaaggagg aggagggggc cgagaagagc agttcaccgt ttgcggccac catgggagaa
      961 gacgacgccg cgctccgggc aagcggcagg gggctctcgg acccgtgggc tgactcggtg
     1021 ggagtgagac cccgcaccac ggagcgccac atcgcagtgc acaagaggct tgtgctggct
     1081 tttgccgtgt ccatcgtggc actgctggct gtcaccatgc tggccgtact gctcagcctg
     1141 cggttcgacg agtgtggagc gagcgcggcg atgccgggca ccgacggtgg cctcggaggc
     1201 ttccctgagc gtggtggcaa cagcagctac ccaggatctg cccggcgcaa ccaccacgcg
     1261 ggtgaggaat cctcgcagcg tgagatcggc gaggtgggca ccgcggggac cccgtctgcc
     1321 catccgccgt cggaggaaga gcaggagcag tggcagccct ggactcagct gcgcctatcc
     1381 ggccacctta agccgctgca ctacaatttg atgctcaccg ccttcatgga gaacttcacc
     1441 ttctctgggg aggtcaacgt ggagatcgcg tgccagaacg ccacccgcta cgtggtactg
     1501 cacgcctccc gggtggcggt ggagaaggtg caagtagcgg aagaccgggc gttcggggct
     1561 gtcccggtag caggcttttt cctctaccca caaacgcagg tcttggtggt ggtgctgaat
     1621 agaaccctgg atgcgcagag gcactacaat ctgaagatta tctacaatgc cctgatagag
     1681 aacgagcttt tgggcttctt ccgcagctcc tacgtgatcc acggggagag aagattcctc
     1741 ggtgttactc agttttcacc tacacatgcc aggaaggcat ttccatgttt tgatgaacca
     1801 atctacaagg ccactttcaa aatcagcatc aaacatcaag caacctattt gtctctctcc
     1861 aacatgcccg tggaaacatc tgtgtttgag gaagatggat gggtaacaga tcacttttca
     1921 cagacccctc tcatgtccac atattattta gcctgggcga tttgcaactt cacatacaga
     1981 gaaactacta ccaagagtgg ggttgtagtt cgattatatg caagacccga cgctatcaga
     2041 agaggatccg gggactatgc tctccacatt acaaagagat taatagaatt ttatgaagac
     2101 tactttaaag tgccctattc tttgccaaaa ctagatcttt tagctgtgcc taagcatcct
     2161 tatgctgcta tggagaactg gggactaagt atttttgtgg aacaaagaat actgctggat
     2221 cccagtgtgt catctatttc ttatttgctg gatgtcacca tggtcattgt tcatgaaata
     2281 tgtcaccagt ggtttggtga ccttgtgacc ccagtgtggt gggaagatgt gtggttgaag
     2341 gaaggctttg ctcactactt tgaatttgtg ggtacagact acctctaccc ttcctggaac
     2401 atggaaaagc agaggtttct gacagatgtt ctgcatgaag tgatgctgct tgacgggttg
     2461 gccagttccc atccagtatc acaggaagtg ctacgggcaa cagatattga caaagtgttt
     2521 gactggatcg catacaaaaa gggtgctgct ttaataagaa tgctggctaa ttttatgggc
     2581 cattcagttt ttcagagggg attgcaagat tatttaacca ttcacaagta tggcaatgca
     2641 gccagaaatg atctctggaa tacattatca gaggctttaa aaaggaatgg aaaatatgtg
     2701 aacatacaag aggtaatgga tcagtggaca ctccagatgg gatatcctgt tatcaccatc
     2761 ctggggaaca tgacggcaga aaacagaata ctcatcaccc aacagcactt catttacgat
     2821 atcggtgcta aaaccaaagc acttcaactt caaaacagca gttacctgtg gcagattcca
     2881 ttaaccatcg tcgtaggaaa tagaagccat gtgtcttcag aagcaattat ttgggtgtcc
     2941 aacaaatcag aacatcacag aataacatac ttagacaaag gaagctggat ccttggaaac
     3001 atcaatcaaa ctggctactt cagagtcaac tatgacctaa ggaactggag attactgatc
     3061 gatcagttaa tcaggaacca tgaggtactt tctgtcagta accgtgcagg cctgattgat
     3121 gatgccttca gcctggccag ggcaggctat ttgcctcaga atattcccct agagatcatc
     3181 agatacctct ctgaggaaaa ggattttctt ccttggcatg ctgccagccg agctctttat
     3241 cctctggata aattactgga ccgcatggaa aactacaata tcttcaatga atatattcta
     3301 aaacaagtgg caacaaccta cagcaagctt ggatggccaa agaacaactt caatggctct
     3361 gtcgttcaag cttcctatca acatgaagag ctacggaggg aagtgataat gttggcctgt
     3421 agctttggga acaagcactg tcaccagcag gcgtcaactc ttatttcaga ctggatttcc
     3481 agcaatagaa acagaatacc actgaatgtt agagacatcg tctactgcac aggagtgtcc
     3541 ctgctggatg aggatgtctg ggaattcatc tggatgaaat tccactccac cactgcagtt
     3601 tctgagaaaa agatattgct ggaggcgcta acttgcagtg atgacaggaa tttactaagt
     3661 aggcttctga atctgtctct gaattctgag gtggtgttgg atcaagatgc aattgatgta
     3721 ataatccacg tagccagaaa tccacatggc cgagaccttg cctggaagtt tttcagagat
     3781 aaatggaaaa tactaaatac caggtatgga gaagcattat ttatgaattc caaacttatc
     3841 agtggagtta cagaatttct taatacagaa ggtgaactta aagagctaaa gaacttcatg
     3901 aagtcctatg atggggtagc atctgcttct ttctcacgag ccgtggaaac cgtggaagcc
     3961 aatgtgcgct ggaaaaggct ttatcaagat gagcttttcc agtggctggg aaaagccatg
     4021 aggcactaat atccatcaaa tagagaattc accccagaac tgtgtaggag gacctgcttt
     4081 ttgtgggatg aggaggctag ccagaatgtc agtgttaaca cgagggagga ttttttcttt
     4141 cttttctttc tttgttgggg gctatttttc ttgtttcatc aactctacat ttgtgtttcc
     4201 actggatatt cctctgtaaa gaaactcttg aaagtgacac ttgccctggt tacttcagct
     4261 gtacattccc cactgttcgg gaccaagtat gatagtggtg catgttgatc ttgttgtcag
     4321 tttgggaaat cctattcaga atattctgtg catggatata tggtcctgcc cgtgttccgg
     4381 catgcttatt taacatgtcc aatgttgtat gtgaacattt tatatgtaca tccagaatgg
     4441 gcattatgcg aaagcacaaa gattacctat gacaatcagt gttgcaatga cagttaaaaa
     4501 gaaaaaacgt tagaagggaa ttctcttctt tgccatggac aacaggagag gtacaagagc
     4561 tcttatgcaa tctgtatcga ttgtttcagc actcggattg tctggcaatg attactgtgt
     4621 tgctaactca ttttctttga gttaaagctg tgtatacact tgcaaagatg tatagatagt
     4681 gtgtacatat acattatgta catagggaag ccctatatgt atatagtatg ttgtacatgc
     4741 acctgttcaa agaatctctt cagatcaaag gaaatttaac tttttggttc ttttttaaat
     4801 atatatatat atatatatat atatatatat atatataaat gtgtggacac actgaaaagg
     4861 cttcaagaag ttgatctcaa cattcctact ttgtatcttg ctctcttaag tgctgttctt
     4921 cttgtctatg tcctgaagaa tgatctgttc tatcacacga agctgaattc taacaaaata
     4981 tacatgtaaa ctaaccacac tgcaaatcct cttaggtcag tggcaatatt ttgttgtttt
     5041 ctgtaattat gttggtcaac attttctact gcataaagcg attttgggta aacttagaat
     5101 gatcgctgct aagaaaatgt ttatgtacag cttctctgtc tttctaagtt tcgctactgc
     5161 agagttaaac tctctctgtg tgttgatctt tgtgtgtact cctgtcctcc attaagcgac
     5221 catacctcaa caggtctatt agtacttaat gaccttctat gccagttgtg ccctccctta
     5281 agacacaaag ttgcttttat gctatgttac tgtacaaagc ctttggaaat ttgcaccccc
     5341 gttctaccta ggaattttga agagtcagtt ctatggctgt gtgtaacaca actagatttc
     5401 actgcttctc tcacccaata ggcctgctcc ctgcttcaga atcaacatat cttaagttta
     5461 acattgtaaa attcctatat aattattata ttgagtcaac tattgtgtaa cccctttttg
     5521 gttttcctgt gatgttcagc ttttccaaat gttgcagttc agagactgtc attcaatgct
     5581 ggaaaaaaat tctttataaa atagaggtca tagaattatt tttgtttgta gaaatgaaaa
     5641 gcaatttccc aatccataga ctacataaaa acaaaatttg atcctgatca tgtaagcagt
     5701 taatgttcat ttcttacagc tataattttc aatacttccg tcaattaaaa tacatgaatt
     5761 ttaagtactg tgtggtacta aagagtcatg gtcatgccta atgtgaaacc tgagatttaa
     5821 gtcacttact gaaagtgagc aagttttaaa aatataatct tcaaaactag tatacagcca
     5881 ggaagaccct aaatatctat gatagcattg gtgaattcat tcatagactg aaactgccta
     5941 aaggggcaga cataggtgtc ttgttttgct ccctgaaata ttgcaaattt tttgagttgg
     6001 gggtggtgct tttcattttg agtgagtggt cgcttccgac tgactttgag tttgaaggtc
     6061 tttcttgtct ttcttagttc tctttcctcc ccatttctaa cagttgtcat cctcattcag
     6121 tgttttagtg gccttgaaca caactgcttt agctatgatc taaatcctca gtgtataatt
     6181 atgtgaaatg tttatacctc agtcctgtgg taccaacggc gtcgagatgg cagttttgcg
     6241 ctacacattt ttcagttatt gaacctttcc aacgtttaaa ttatgccagg tttgaagtct
     6301 ccttttagtt gtttaaataa gtaatatttt caaaataata aaaaacaatg atatctcttg
     6361 gaatattctg taaaatgtag ctataaaatt atattttcta ctttagagtt tttacctttt
     6421 tctcttgtgt gttttacaaa tcaattatct acatgaaata acaatgtttt gaaagtg
//
