LOCUS       NM_001399412            6673 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus translocation associated membrane protein 2
            (Tram2), mRNA.
ACCESSION   NM_001399412 XM_002730009
VERSION     NM_001399412.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Chen,Q., Denard,B., Lee,C.E., Han,S., Ye,J.S. and Ye,J.
  TITLE     Inverting the Topology of a Transmembrane Protein by Regulating the
            Translocation of the First Transmembrane Helix
  JOURNAL   Mol Cell 63 (4), 567-578 (2016)
   PUBMED   27499293
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
REFERENCE   3  (bases 1 to 6673)
  AUTHORS   Stefanovic,B., Stefanovic,L., Schnabl,B., Bataller,R. and
            Brenner,D.A.
  TITLE     TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump
            Serca2b and is necessary for collagen type I synthesis
  JOURNAL   Mol Cell Biol 24 (4), 1758-1768 (2004)
   PUBMED   14749390
  REMARK    GeneRIF: TRAM2, as a part of the translocon, is required for the
            biosynthesis of type I collagen by coupling the activity of SERCA2b
            with the activity of the translocon.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On Jan 7, 2022 this sequence version replaced XM_002730009.7.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.38581.1,
                                           SRR26643287.22260.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               JAXUCZ010000009.1  30994915-30995162   c
            249-312             JAXUCZ010000009.1  30952385-30952448   c
            313-422             JAXUCZ010000009.1  30933087-30933196   c
            423-539             JAXUCZ010000009.1  30932464-30932580   c
            540-598             JAXUCZ010000009.1  30926554-30926612   c
            599-683             JAXUCZ010000009.1  30925697-30925781   c
            684-754             JAXUCZ010000009.1  30925136-30925206   c
            755-859             JAXUCZ010000009.1  30923781-30923885   c
            860-1003            JAXUCZ010000009.1  30923356-30923499   c
            1004-1167           JAXUCZ010000009.1  30922428-30922591   c
            1168-6673           JAXUCZ010000009.1  30915624-30921129   c
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q13"
     gene            1..6673
                     /gene="Tram2"
                     /note="translocation associated membrane protein 2"
                     /db_xref="GeneID:100361830"
                     /db_xref="RGD:1589151"
     exon            1..248
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..1241
                     /gene="Tram2"
                     /note="translocating chain-associating membrane protein 2"
                     /codon_start=1
                     /product="translocating chain-associated membrane protein
                     2"
                     /protein_id="NP_001386341.1"
                     /db_xref="GeneID:100361830"
                     /db_xref="RGD:1589151"
                     /translation="MAFRRRTKSYPLFSQEFVIHNHADIVFFLVLCVLIGLMFEVTAK
                     IAFLCILPQYNVSVPTADSETVNYHYGPKDLATILFYILITIILHAVVQEYILDKISK
                     RLHLSKVKHSKFNESGQLVVFHLSSVAWCLYVVATEGYLSNPRSLWEDYPHVYLSFQV
                     KFFYLGQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLLHIAGAYLLNLSRLGLILL
                     LLQYSTEALFHMARLFYFADENNERLFNAWAAVFGVTRLFIFTLAVLTIGFGLARVEN
                     QVFDPEKGNFNTLPCRLGMLLLVCVAQAWLMWRFIHSQLRHWREYWKEQSAKRRVSAV
                     PRLPARLLKREPGFHENGVVKTENGTSSRTKKLKSP"
     exon            249..312
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            313..422
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            423..539
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            540..598
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            599..683
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            684..754
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            755..859
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1003
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1167
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..6673
                     /gene="Tram2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcggggccc gaagttgttc cgcgctgggc tgcggggctg gcgggcggac cgggccgggc
       61 cagagaagtt ttcgctgcag ctgctgagcg agggttcggg cccccgcagg cggtgcacgc
      121 ccgctgccat ggctttccgt aggaggacga aaagttaccc gctcttcagc caggagttcg
      181 tcatccacaa ccatgcagac atcgtcttct tcttggtgct ctgtgtcctc atcgggctta
      241 tgttcgaggt cacggccaag attgccttcc tatgtatttt acctcagtat aacgttagtg
      301 tgcctacagc agacagcgag acagtgaact accactatgg cccaaaggac ctggccacca
      361 tcttgttcta catcttaatc accatcatct tgcatgctgt ggttcaagag tacatcttag
      421 acaaaatcag caaacgcctt catctctcca aggtcaaaca cagcaagttc aatgaatctg
      481 gacagctggt tgtctttcat ctcagctcag tggcatggtg cctctatgtg gtggcaacgg
      541 aaggctattt atcaaaccca agaagcctct gggaagatta cccgcatgtg tacctctcct
      601 tccaggtgaa gtttttctac ttgggtcagc tggcgtactg gctgcacgcg ctacctgaac
      661 tctatttcca gaaggtacgg aaggaagaaa tcccccgcca gctacagtac atttgcctgt
      721 acctgctcca catcgctggg gcgtacctct tgaacctcag ccgcctgggg ctgatcttgc
      781 tcctgctgca gtactccact gaggccctct ttcacatggc tcggctcttc tactttgccg
      841 acgagaacaa cgagaggctg tttaacgcct gggctgctgt gtttggggtc acacgtctct
      901 tcatcttcac cctcgctgtg ttgaccattg gcttcggatt ggctcgggtg gaaaaccagg
      961 tgtttgaccc cgagaaaggg aactttaaca ccttgccttg caggctcggc atgctgctgc
     1021 tggtatgtgt ggcccaggcc tggctcatgt ggcgtttcat ccactcccag ctgcgacact
     1081 ggcgggagta ctggaaagaa cagagtgcca agcgcagagt ctctgcggtc ccccggcttc
     1141 cagccaggct cctcaagaga gaacctggtt tccatgaaaa tggagtggtg aagacagaga
     1201 acggaacctc ctcacggact aagaaactca agtctcccta gcgccattgt gctgaaaaca
     1261 gggatcctct gggtgagggc ctggcagaga tgaggactcc tgacccccaa ctcagactct
     1321 ctgcccttct ggccgtactc catctccacc agcagaaacc ttggcatcaa agggggagct
     1381 cctctctacc ttctagcctt tctcttcttt cataaactgt tctcaataaa gccaaaaaaa
     1441 cttctattct tgctcccttg agccacctca tggcctcact tctgtcctat cttgggtttt
     1501 ctcagaggcc tgccttcttc tagtcgtctt cctgctctca ctctcactct ctcgctcgct
     1561 ctctctcctc gaccctcaat gcctccattt tcctcccctc ctctcctctc ctctcctctc
     1621 ctctcctctc ctctcctctc ctctcctctc ctctcctctc ctctcctctc ctctcctctc
     1681 ccctcccctc ccctcccctc ccctcctctc ctctcctctc ctctcctctt tctctcttcc
     1741 ttcctgactg cttcttttcc tcctttcttc ctgactttaa tgtgcaattt ctctgatttg
     1801 tacaacggga gggagctagg acggcagtcc tgcccttcgg gcccagccag gacttgccaa
     1861 tctggaagcc tctcgccaag gagcttcccc tagagctggt ggcagaccct cggtggccct
     1921 tgtggatggc aggaggagga aaagccatcc ctctcggatt acaaaattct tggtgctgac
     1981 cgacagacaa gatgtaggat tctggctcta cacgtcggac cccgaagcgt ttcgctctcc
     2041 atagtgactt aatccagaaa gacagtgtta attaactgtt gagtcccagg actccaaaga
     2101 acttccagtg cagcatttga attggaaacc agcctgcctt cgctctatgg ggggtcgtgc
     2161 taaggaggat gtgccttccc acttgttcca caagagcaaa gcacgtcaaa cagctgcctc
     2221 ttctagaaag gtggcttggc caaaacaaaa tcagacaaac aaaaaacaac agaagagtcg
     2281 atctggacca ttctccagca gtgccaactg ctgtagtttc attacctgtc caccagcatc
     2341 tgaaaccaaa ccaaatggca gttatgaatc tgcccaggac atgctgttcc caagtctaga
     2401 aacagcctgc cccagccggg gccagacttg caacctcacc atctctctgc agaaacacga
     2461 agccccaagg actccgatcg cagtgtgttc ccctggtgtc ccaaggaggg tcacctggct
     2521 gccctccctc tggcctcact gagtgttctt taaagttgtc agtgtcattt gtcaagcaca
     2581 cctagggcct gacaccggga tgcctcaggg tcactcttct ggctgcgtga gccaacgcag
     2641 ccatgtccac aggagtctgt gacccattcc ctttggtgtt ctttactctc tcttctacct
     2701 gaagatgggg tcatcgggcc cttatggtac cttgtgagga cttccaggat gtatgtaggc
     2761 tttaaagagt ccccacactc tgtgctggag tataggggag cagttcttgg tgacacagga
     2821 atgttaatgt tacctgatat acttggtctt cctttgacac catattcaag accagggctt
     2881 gagtttttcc ccatatacag ggacatgggt cttcatgaag gcagtttccc tctccaagcc
     2941 actaacgatg tacataagat ctaactcacc agtttttgag gagtttggtt ccaaacatcg
     3001 acgtggcaat ttttttgttt ttgttttttt tttttgtttg tttgtttgtt tttttctctc
     3061 tgtgtccagg ttaagcatac agaatagaaa tgagatcccc ttggcttctt ttgtgtgagc
     3121 ttaggaagca agatagaatt ttgcaagtac atttctctat tgcctcctgg cccttctctg
     3181 ggcatcccca cagaattgat ctggctggca ccttttagcc gtgcagccca cccatcctca
     3241 tagggatttt aatcaagctt acgctttgag atgctgcctg tagcgatgtg aaagttcagc
     3301 tagtggctcc caagatcgcc tcaccccgtc agtttaagtg tcctccggga ccatccccag
     3361 gttaagggcc tgtctcccta agacactggc aagccctttg ccagctcact gtgggagctt
     3421 tgtctctagg ctgaactatt gagttactcc ctcagtaaat atttgggaca agcatgagat
     3481 atgtggaggt cttggagaaa cagaagtaca aaggccagct gtctaggatc cagtggtcct
     3541 gtcgctgtgg cggggtgggg gtggggggtt gctactcttt cccagtacac ttagatgact
     3601 gagatggtgt ggatctgttc cttgagtgct tgcaggcttc ctttgctgct tgtatgttgg
     3661 gtgttatcac cgagaatcca agtgttggga ttgaccaagg ctcttctgga acatatgggt
     3721 ttggtcagtg gtaggagttt agagcattca tttcagaaaa gctgagacca agggcaacac
     3781 ctccccaggc ttgacttctg ggatgatttc gggcttgggg gaactagggg gccctttctc
     3841 atccacccag cctctctggc gttgatgtta taggaaagcc aagactttgt agagttagca
     3901 cagaccttgt tgccagaatc acagcaacca agttctacac catcctgcgt gtgcgcaggc
     3961 tagcttataa tttgggtgcc ctggccccat tctttgattt tatgcaggct ctcccatccc
     4021 ccaaaatacc acacccttag tgagggacct gtcatgctcc tccaagtttc agatgttcag
     4081 cacaatctac caggaccaga agatgctaat cactacctca gcagccactg cctgtctctc
     4141 agtgtccctg gaacgccagc aggcgccagg tggcagagtg gtggatggag agctccctgt
     4201 tactagttgc cctccccaga ggtgtggcga tgggacggtt acttttacaa gctatccagg
     4261 tttggatgcc tacgctatga attagctcct tgtgtccctc ctacattctg aggtggtcct
     4321 gggagaaaga gaagcaaact aaaactttct gccattctag ccagtcctag ccctgcgtac
     4381 ctaaatggtt ttcaggcagc atcatctggt gcatcctaag ggtggactta agtcacttaa
     4441 atttagatga cagtgttctg gctactcaca ccatgctttt gtggagttcc tgggactgta
     4501 agagcccctg aaggtggaga ccttttaagc cccctttggc attttttttt tctgagtggt
     4561 tttttttccc cattttccaa aaagcctaca aagttctctt agattccctg catccttaaa
     4621 gaaacctgct gactcccttg cagctaggga agacctccat tggcgtttgt ctttaatggc
     4681 tctcagccgg ttgagtttgc agaagtctgg gacggggagg aggtataggg tctcagctta
     4741 ggtaactttc tctgcctgag ggcctctgtg tctccaggag acagatttcc attcttggtg
     4801 tctggcctct gttcttcact ggtgtggaat ggacttgaag cctgtgagtt cgaacagtga
     4861 gctggtcctc tgcctccctc gtgctcccga gcttacacct tctttcctca gaacaggatt
     4921 taaaggagga tatcagtgtc agacttttga aagcatctgt agaatccttc tccagcccat
     4981 ggctagcgtt aggaggatgc tggtaccaac ttctcgccat ttatccctgg ctttttatgc
     5041 tagcgttagc tccattgcat ccgcttccaa agctctgcag atgagaagaa aagtcatgcc
     5101 gagcagcctc tacgaacgtc ccgttcttat ccttgttctt tcatgtaaaa gtgccttata
     5161 ttgccttgtg gccttcactg gtactgtatg gctgggcccc ccaactgcca gggctctagg
     5221 gctgactggc atacacaggc aatgacacca tacctcaaca cagatccagg ggaaagggac
     5281 atgggttgac agttttgtct catttgttct gaccctcttc tccctcgcac ataatgtaag
     5341 accttgtgta ctctctggtt tattctaagt tccccttgtc tgttgccttc tggcttgacc
     5401 gggtggctat ggaaactctc gaggtggccc agctgcaggc acccatgctt cctatttcat
     5461 aagggaagct tgggggttgg gaagaagggt cacccctgtg tacctctggc cctatgtggt
     5521 gagccctgct ttttcggttt cctcattccc accccagatt agaaacagca ccctcttccc
     5581 catctgtttt acttccctgt agcccaaagt ttgtgtgagg aatgtattta cttgtactcg
     5641 ctggtttcaa gtcttagctc attggtaacc tgtttggcag tgcctatggt ctggtagctg
     5701 cagagctcct ggattcctgt cagggttatc tcgtgttcct agttccgcac caacccacct
     5761 ccctgaccct tgccttgcct gcctgcccat tcctgatatc ccgctattcc gggttgtgca
     5821 ataaccctcc cagtggtctt ttccacaggc ctttgtctct taccgcatcc tgcagtaaca
     5881 ggagagaggg gacctacggg aggtgtcatc atcccttaca gaagcgtcag tcccttcgtt
     5941 gttagctgtc attctcatgg tcttgggtgg gtttctgtgg caatcttata gaatgtgccc
     6001 tatcgtctaa gaaatcttta cattgttttc aaaaataaaa taaaaaatgt gttgaagaga
     6061 aaaaaaaaaa aaccatcctc agactgggac agtgtcgatg tctgggaata agatcagtct
     6121 ggagtcgctg acatcaccgt caccaccaaa ggcattccag ggtttcttgt tcctgtgcaa
     6181 aggatgggcc atgtttccaa gaatcagcca cagtggtcac agtggtgagc cctgctcccc
     6241 cacaatggga tctggctgtg gtggtgagtg ccgacattct ttggggtaca gattcatgtg
     6301 tgattaaacc tcacttttcg gttactctgt ttgtcaagca ccgaaaggat gagcgttgga
     6361 gctggtgccc ctgcgtgact cacaacgcaa atgtggacac attgactgtt tctgcagtca
     6421 cctgttcatg tgatggacca aattcagcaa ggaactcaag gaaaattcgt acctaccgta
     6481 tttatgcttt cttattaaag ggctgggggg aggggcgtct ttttactttt ggtgaaattt
     6541 ttattggtcc ttttcccact gtttctgtct ggttttagaa acaagctaca gttttgtatg
     6601 gattttttta aatgtacatt ttgaaacaaa tgatcaaata ttttctgaat aaagggcata
     6661 gaagtattgg tca
//
