LOCUS       NM_001107790            3169 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus TPX2, microtubule nucleation factor (Tpx2), mRNA.
ACCESSION   NM_001107790 XM_001060281 XM_001060351 XM_230735
VERSION     NM_001107790.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Roostalu,J., Cade,N.I. and Surrey,T.
  TITLE     Complementary activities of TPX2 and chTOG constitute an efficient
            importin-regulated microtubule nucleation module
  JOURNAL   Nat Cell Biol 17 (11), 1422-1434 (2015)
   PUBMED   26414402
  REMARK    Erratum:[Nat Cell Biol. 2015 Nov;17(11):1512. doi: 10.1038/ncb3265.
            PMID: 26515019]
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Wei,J.H., Zhang,Z.C., Wynn,R.M. and Seemann,J.
  TITLE     GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2
            and Capturing Microtubules
  JOURNAL   Cell 162 (2), 287-299 (2015)
   PUBMED   26165940
REFERENCE   3  (bases 1 to 3169)
  AUTHORS   Bibby,R.A., Tang,C., Faisal,A., Drosopoulos,K., Lubbe,S.,
            Houlston,R., Bayliss,R. and Linardopoulos,S.
  TITLE     A cancer-associated aurora A mutant is mislocalized and
            misregulated due to loss of interaction with TPX2
  JOURNAL   J Biol Chem 284 (48), 33177-33184 (2009)
   PUBMED   19801554
REFERENCE   4  (bases 1 to 3169)
  AUTHORS   Mori,D., Yamada,M., Mimori-Kiyosue,Y., Shirai,Y., Suzuki,A.,
            Ohno,S., Saya,H., Wynshaw-Boris,A. and Hirotsune,S.
  TITLE     An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite
            elongation by modulation of microtubule dynamics
  JOURNAL   Nat Cell Biol 11 (9), 1057-1068 (2009)
   PUBMED   19668197
REFERENCE   5  (bases 1 to 3169)
  AUTHORS   Zhao,B., Smallwood,A., Yang,J., Koretke,K., Nurse,K., Calamari,A.,
            Kirkpatrick,R.B. and Lai,Z.
  TITLE     Modulation of kinase-inhibitor interactions by auxiliary protein
            binding: crystallography studies on Aurora A interactions with
            VX-680 and with TPX2
  JOURNAL   Protein Sci 17 (10), 1791-1797 (2008)
   PUBMED   18662907
REFERENCE   6  (bases 1 to 3169)
  AUTHORS   Bird,A.W. and Hyman,A.A.
  TITLE     Building a spindle of the correct length in human cells requires
            the interaction between TPX2 and Aurora A
  JOURNAL   J Cell Biol 182 (2), 289-300 (2008)
   PUBMED   18663142
REFERENCE   7  (bases 1 to 3169)
  AUTHORS   Cassimeris,L. and Morabito,J.
  TITLE     TOGp, the human homolog of XMAP215/Dis1, is required for centrosome
            integrity, spindle pole organization, and bipolar spindle assembly
  JOURNAL   Mol Biol Cell 15 (4), 1580-1590 (2004)
   PUBMED   14718566
REFERENCE   8  (bases 1 to 3169)
  AUTHORS   Bayliss,R., Sardon,T., Vernos,I. and Conti,E.
  TITLE     Structural basis of Aurora-A activation by TPX2 at the mitotic
            spindle
  JOURNAL   Mol Cell 12 (4), 851-862 (2003)
   PUBMED   14580337
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            [WARNING] On Oct 22, 2024 this sequence was replaced by
            NM_001107790.3.
            
            On Nov 27, 2020 this sequence version replaced NM_001107790.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR26643292.14968.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN06680417, SAMN06680424 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-329               JAXUCZ010000003.1  161778720-161779048
            330-499             JAXUCZ010000003.1  161796928-161797097
            500-622             JAXUCZ010000003.1  161801633-161801755
            623-749             JAXUCZ010000003.1  161802349-161802475
            750-881             JAXUCZ010000003.1  161803823-161803954
            882-1001            JAXUCZ010000003.1  161804751-161804870
            1002-1123           JAXUCZ010000003.1  161807856-161807977
            1124-1275           JAXUCZ010000003.1  161808786-161808937
            1276-1444           JAXUCZ010000003.1  161810063-161810231
            1445-1583           JAXUCZ010000003.1  161812101-161812239
            1584-1800           JAXUCZ010000003.1  161813561-161813777
            1801-1896           JAXUCZ010000003.1  161814756-161814851
            1897-2073           JAXUCZ010000003.1  161816149-161816325
            2074-2220           JAXUCZ010000003.1  161817136-161817282
            2221-2332           JAXUCZ010000003.1  161818203-161818314
            2333-2520           JAXUCZ010000003.1  161818889-161819076
            2521-3169           JAXUCZ010000003.1  161820293-161820941
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q41"
     gene            1..3169
                     /gene="Tpx2"
                     /note="TPX2, microtubule nucleation factor"
                     /db_xref="GeneID:311546"
                     /db_xref="RGD:1311894"
     exon            1..329
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            330..499
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     CDS             394..2628
                     /gene="Tpx2"
                     /note="TPX2, microtubule-associated protein homolog; TPX2,
                     microtubule-associated, homolog"
                     /codon_start=1
                     /product="targeting protein for Xklp2"
                     /protein_id="NP_001101260.1"
                     /db_xref="GeneID:311546"
                     /db_xref="RGD:1311894"
                     /translation="MAQVPTAYSFDAPTDFINFSSLDAEEDTENIDSWFDEKANLENK
                     FLRQSGIGEPFQGKTSLRKAKLQQGCVTPLKAVDNTYYKETEKENLQKQSIPSNDCPS
                     LNAKRAVTGTTPIQPQRRSVRLSAQKDLEQKEKNHVASVEMKAKRCTAPTTDYPPNKK
                     MKVSHKKKPEEEEEGSAPATSRKDETLERAKGKHTVPAVPPAREKTVKSTEEQELEKS
                     LRMQQEVVEMRKKNEEFKKLALAGPGQPVKKSTSQVTKTVDFHFLTDERIKQHPKSQE
                     EYKEVNFMSELRKHSSTPARGTRGCTIIKPFNLSKGKKRTFDEAASTYVPIAQQVEAF
                     HKRTPNRYHLRNKKDESLLPSKPVNKIVRDPQTPILQTKYRARAVTCKSAAEKEAEEL
                     EKLQQYKFKARELDPRIFESGPILPKRPPVKPPTQPVGFDLEIEKRIHERESKKKPED
                     EQFEFHSRPCPTKILEDVVGVPEKKVIPATVPKSPVFALKNRIRVPVKEDEEEEKPVV
                     IKAQPVPHYGVPYKPPIPETRNVEVCPFSFDTRDKERQLQKEKKIKEMQKGEVPKFKA
                     LPVPHFDTINLPEKKVKNATQAEPFSLETDKRGAYKAEIWKHQLEEEQKQQKEAACFK
                     ARPNTVIFQEPFVPKKEKKSAAENPSGSLVQEPFQLATEKRAKERQELEKKMAEMEAW
                     KLQQLAEVRQQEEEQQKEELARLRKELVHKANPIRKYSAVEVKSSELPLTVPVSPKFS
                     TRFQ"
     exon            500..622
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            623..749
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            750..881
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1001
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1123
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1275
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1444
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1583
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1800
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1896
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2073
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2220
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2332
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2520
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
     exon            2521..3169
                     /gene="Tpx2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgattggtg catttgaccg gtgtctgagt tcaaattttc aaccgtacta gttgtgtccg
       61 ggagccgtct taggcgctgg cttcttagag ttggtgcaag ctgaagccgg gaaagtagtg
      121 tgaaggtgag gggaagcagc ggtgcgccgg gagccattcc tcagttttct gagagggtgg
      181 aggagtccgc ggggcgggcc atgccagcct agacagcgcg cgggcttcgg acgccggggt
      241 cgggacttgg gagtgaagtc tcagattcgg cttcagtatt cctgggaacc ccggagcaga
      301 ggttgctcca ggctgtcagc tactagacga ggacgatcta ttgagtggag tattagaatc
      361 ctggaaaaag tgtgttctgc actaggtggg gcaatggccc aagtcccaac agcttactcg
      421 tttgatgccc ccaccgactt tatcaacttt tcatctttgg atgctgaaga ggacactgaa
      481 aatatagact catggtttga tgagaaggcc aacttggaga acaagtttct tcggcagagt
      541 ggaataggag aaccttttca ggggaagacg tccttgagaa aagccaaact tcaacaaggc
      601 tgtgtcacac cattgaaggc agttgataac acttactaca aagagacaga aaaggaaaat
      661 cttcagaagc aatctatccc atcaaatgat tgtccttccc tgaatgctaa gagagctgtg
      721 acaggaacta ctcccatcca gcctcagaga agatctgtta gactctctgc tcagaaggat
      781 ttggagcaga aagagaaaaa ccatgttgcc tctgttgaaa tgaaagccaa gagatgcact
      841 gctcccacga ctgattatcc ccccaacaag aaaatgaaag tgtctcataa aaagaaaccg
      901 gaggaagaag aggagggcag tgctccagct acttcaagaa aggatgaaac tctggagagg
      961 gccaagggca aacacactgt gcctgctgtg ccgcctgcca gggagaagac tgtaaagagt
     1021 actgaagagc aggagttaga gaagagtctg agaatgcagc aggaagtggt agagatgcgg
     1081 aagaagaatg aagagtttaa gaagctggct cttgcagggc cagggcaacc tgtgaagaaa
     1141 tccaccagcc aggttaccaa gacagttgac tttcacttcc tcacagatga gagaatcaaa
     1201 caacatccca agagccagga ggagtataag gaagtgaact tcatgtccga acttcgaaag
     1261 cattcttcca ctcctgcccg agggaccaga ggatgtacca tcattaagcc tttcaatctg
     1321 tccaaaggaa agaaaagaac atttgacgaa gcagcgtcta catatgtgcc cattgcacag
     1381 caggttgaag ccttccacaa acgaacccct aatcgatacc atctgaggaa caagaaggac
     1441 gagagcttgt taccctccaa acctgtgaac aagattgtac gagaccccca gacccctata
     1501 ctgcagacca aataccgtgc aagggctgtg acctgcaaaa gtgcggcaga gaaggaggcc
     1561 gaggaacttg agaaactgca acaatacaaa ttcaaagcac gggaacttga tcccagaatt
     1621 tttgaaagtg gccccatctt gcccaagaga ccacctgtta agcctcctac ccagcctgtt
     1681 ggttttgatt tggaaattga gaaacgaatt catgagcgag agtcaaagaa aaaacccgaa
     1741 gatgaacaat ttgaatttca ttccagacct tgccctacta agatcttgga agatgttgtg
     1801 ggtgttcctg aaaagaaggt tattccagcc actgtcccta agtcaccagt ttttgcactg
     1861 aagaacagga tccgagtgcc tgtcaaagaa gatgaggaag aggagaaacc agtagtgata
     1921 aaagctcaac ctgtgccaca ttatggggtg ccttataaac cccccatccc agagacaaga
     1981 aatgtggagg tgtgcccgtt ctcctttgat acccgggaca aagaacgcca gttgcagaag
     2041 gagaaaaaaa taaaagaaat gcagaaagga gaggtgccca agttcaaggc acttcctgtg
     2101 ccccattttg acaccattaa cctgccagag aaaaaggtaa agaatgcgac tcaagccgag
     2161 cctttctcct tggagacaga caagagaggt gcctacaagg ccgagatatg gaagcaccag
     2221 ctggaagaag agcagaagca gcagaaggaa gcagcttgct tcaaggctcg tccaaacact
     2281 gtcatcttcc aagagccctt tgttcccaag aaagagaaaa aatcagctgc tgagaaccct
     2341 tctggttctc tagttcagga accttttcag ctggccaccg agaagagagc caaagagcgg
     2401 caggagctgg agaagaaaat ggctgagatg gaggcctgga aactgcagca gttggcagaa
     2461 gtcaggcagc aggaagagga gcagcagaag gaggagctgg ccaggctccg gaaggaactg
     2521 gtgcacaagg caaatccaat ccgaaagtac tcagcagtgg aggtgaaatc cagtgagctg
     2581 cctctgaccg tgccggtgtc tcctaagttc tccactcggt tccagtagaa gccgccgcat
     2641 gtacaccaca ggcacagaga acacgttttc agactcacct acgtgtgtgg aaacatacct
     2701 gagaactgtg gaggccaggt tttgaagatc gagttacatg gagactttga agctaatgag
     2761 acagcactca tttagactca tcagacttcc tgtagttatt tcctgcagac attagctggc
     2821 cctttcctgt tggtcataac tctggccagg acttcaacta ttcattttgg catagtctac
     2881 ttttcaccta atctccccct tctccccctt tatatatgta ttttaagcat aaaataaagg
     2941 cagtcaagtc ctaggtctgg tttcagtcac agtgtaagtg agaagcagcc agtctgtagg
     3001 attccttctt cagaagagtt tatgggggag ccctgcactg tgaggcagca gggaacctcc
     3061 gtaagggcac caaggaagtc gactgcatgg accccgagag accccccgga gacgctgctg
     3121 ggcatgttct tcattgtcgg tgcagacagt ggaatgtcac agcctacag
//
