LOCUS       NM_019322               1225 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus tryptase alpha/beta 1 (Tpsab1), mRNA.
ACCESSION   NM_019322
VERSION     NM_019322.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Maun,H.R., Liu,P.S., Franke,Y., Eigenbrot,C., Forrest,W.F.,
            Schwartz,L.B. and Lazarus,R.A.
  TITLE     Dual functionality of beta-tryptase protomers as both proteases and
            cofactors in the active tetramer
  JOURNAL   J Biol Chem 293 (25), 9614-9628 (2018)
   PUBMED   29661938
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Barallobre-Barreiro,J., Gupta,S.K., Zoccarato,A.,
            Kitazume-Taneike,R., Fava,M., Yin,X., Werner,T., Hirt,M.N.,
            Zampetaki,A., Viviano,A., Chong,M., Bern,M., Kourliouros,A.,
            Domenech,N., Willeit,P., Shah,A.M., Jahangiri,M., Schaefer,L.,
            Fischer,J.W., Iozzo,R.V., Viner,R., Thum,T., Heineke,J.,
            Kichler,A., Otsu,K. and Mayr,M.
  TITLE     Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin
            Activity in Human Atrial Fibrillation
  JOURNAL   Circulation 134 (11), 817-832 (2016)
   PUBMED   27559042
REFERENCE   3  (bases 1 to 1225)
  AUTHORS   Barallobre-Barreiro,J., Oklu,R., Lynch,M., Fava,M., Baig,F.,
            Yin,X., Barwari,T., Potier,D.N., Albadawi,H., Jahangiri,M.,
            Porter,K.E., Watkins,M.T., Misra,S., Stoughton,J. and Mayr,M.
  TITLE     Extracellular matrix remodelling in response to venous
            hypertension: proteomics of human varicose veins
  JOURNAL   Cardiovasc Res 110 (3), 419-430 (2016)
   PUBMED   27068509
REFERENCE   4  (bases 1 to 1225)
  AUTHORS   Duchesne,E., Tremblay,M.H. and Cote,C.H.
  TITLE     Mast cell tryptase stimulates myoblast proliferation; a mechanism
            relying on protease-activated receptor-2 and cyclooxygenase-2
  JOURNAL   BMC Musculoskelet Disord 12, 235 (2011)
   PUBMED   21999702
  REMARK    GeneRIF: Tryptase can stimulate myoblast proliferation and this
            effect is part of a signaling cascade dependent on PAR-2 activation
            and on the downstream activation of COX-2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1225)
  AUTHORS   Didangelos,A., Yin,X., Mandal,K., Baumert,M., Jahangiri,M. and
            Mayr,M.
  TITLE     Proteomics characterization of extracellular space components in
            the human aorta
  JOURNAL   Mol Cell Proteomics 9 (9), 2048-2062 (2010)
   PUBMED   20551380
REFERENCE   6  (bases 1 to 1225)
  AUTHORS   Huang,C., Wong,G.W., Ghildyal,N., Gurish,M.F., Sali,A.,
            Matsumoto,R., Qiu,W.T. and Stevens,R.L.
  TITLE     The tryptase, mouse mast cell protease 7, exhibits anticoagulant
            activity in vivo and in vitro due to its ability to degrade
            fibrinogen in the presence of the diverse array of protease
            inhibitors in plasma
  JOURNAL   J Biol Chem 272 (50), 31885-31893 (1997)
   PUBMED   9395536
REFERENCE   7  (bases 1 to 1225)
  AUTHORS   Lutzelschwab,C., Pejler,G., Aveskogh,M. and Hellman,L.
  TITLE     Secretory granule proteases in rat mast cells. Cloning of 10
            different serine proteases and a carboxypeptidase A from various
            rat mast cell populations
  JOURNAL   J Exp Med 185 (1), 13-29 (1997)
   PUBMED   8996238
REFERENCE   8  (bases 1 to 1225)
  AUTHORS   Ghildyal,N., Friend,D.S., Stevens,R.L., Austen,K.F., Huang,C.,
            Penrose,J.F., Sali,A. and Gurish,M.F.
  TITLE     Fate of two mast cell tryptases in V3 mastocytosis and normal
            BALB/c mice undergoing passive systemic anaphylaxis: prolonged
            retention of exocytosed mMCP-6 in connective tissues, and rapid
            accumulation of enzymatically active mMCP-7 in the blood
  JOURNAL   J Exp Med 184 (3), 1061-1073 (1996)
   PUBMED   9064323
REFERENCE   9  (bases 1 to 1225)
  AUTHORS   Eto,I. and Grubbs,C.J.
  TITLE     Separation, purification and N-terminal sequence analysis of a
            novel leupeptin-sensitive serine endopeptidase present in
            chemically induced rat mammary tumour
  JOURNAL   Biochem J 283 (Pt 1) (Pt 1), 209-216 (1992)
   PUBMED   1314562
REFERENCE   10 (bases 1 to 1225)
  AUTHORS   Braganza,V.J. and Simmons,W.H.
  TITLE     Tryptase from rat skin: purification and properties
  JOURNAL   Biochemistry 30 (20), 4997-5007 (1991)
   PUBMED   2036367
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U67910.1 and BC061983.1.
            
            On Jul 12, 2008 this sequence version replaced NM_019322.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U67910.1, BC061983.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06621351, SAMN06680412
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               U67910.1           1-173
            174-1225            BC061983.1         156-1207
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q12"
     gene            1..1225
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /note="tryptase alpha/beta 1"
                     /db_xref="GeneID:54271"
                     /db_xref="RGD:3066"
     exon            1..278
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1039
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /EC_number="3.4.21.59"
                     /note="MMCP-7; tryptase, skin; mast cell protease 7;
                     tryptase beta 1"
                     /codon_start=1
                     /product="tryptase precursor"
                     /protein_id="NP_062195.2"
                     /db_xref="GeneID:54271"
                     /db_xref="RGD:3066"
                     /translation="MDLPSCSLVPGLFCTPQPVGPSPALTSNRARTTHCVRMLKLLLL
                     TLPLLSSLVHAAPSLAMPREGIVGGQEASGNKWPWQVSLRVNDTYWMHFCGGSLIHPQ
                     WVLTAAHCVGPNKADPNKLRVQLRKQYLYYHDHLLTVSQIISHPDFYIAQDGADIALL
                     KLTNPVNITSNVHTVSLPPASETFPSGTLCWVTGWGNINNDVSLPPPFPLEEVQVPIV
                     ENRLCDLKYHKGLNTGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGV
                     VSWGEGCAQPNRPGIYTRVTYYLDWIYRYVPKDF"
     exon            279..444
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /inference="alignment:Splign:2.1.0"
     exon            445..710
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..874
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1224
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1206..1211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
     regulatory      1216..1221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
     polyA_site      1224
                     /gene="Tpsab1"
                     /gene_synonym="Mcpt7; rMCP-7; TPSB1"
ORIGIN      
        1 tagagaagat caaagacagc tgaggtgatc tctcccagag cctccgacat ctgagtgttg
       61 cgctgaagca gagtggccaa gccactagag acctcaagct cctggaatgg acctaccctc
      121 ctgctccctg gttcctggct tattctgcac accacaacct gttgggccta gcccagccct
      181 cacctccaac cgggcccgta ctactcactg tgtccgaatg ctgaagctgc tgctgctgac
      241 actgcctctc ctgtccagcc tggtgcacgc agcccctagt ctagctatgc cacgagaggg
      301 cattgtgggg ggacaggagg cgtctgggaa caagtggccc tggcaggtga gcctgcgtgt
      361 caatgacacc tactggatgc atttctgcgg cggctccctc atccacccac agtgggtgct
      421 cactgcggca cactgtgtgg gaccgaataa ggctgacccc aacaagctca gggtacagct
      481 tcgtaagcag tatctctatt atcatgacca cctgctgacc gtgagccaga tcatctcaca
      541 ccctgacttc tacatcgccc aggatggggc agacattgcc ctgctgaaac tcactaaccc
      601 tgtgaacatt accagcaatg tccacactgt ctccctgcct cccgcctcag agaccttccc
      661 ctcagggaca ttgtgctggg tgacaggctg gggtaacatc aacaatgatg taagcctgcc
      721 accgccattt cctttggagg aggtgcaagt tcccattgta gaaaaccgcc tctgtgactt
      781 gaagtatcac aaaggtctca acacaggtga caacgtccac attgtccgtg acgacatgct
      841 gtgtgctggg aatgaaggac atgactcctg ccagggcgac tccggaggac ctctggtctg
      901 caaggttgaa gatacctggc tgcaggcagg tgttgtgagc tggggtgagg gctgcgcaca
      961 gcccaacagg cctggcatct acactcgggt cacctattac ctggactgga tctaccgcta
     1021 tgtccccaag gacttctgag ttgcatccag ggtgacctct gttgtttcca acatgctgct
     1081 ttctgcctcc ctgcttccct gctccccagc ctgagcccca actctgctct acctgagccc
     1141 ctgaccactt gtgtccctcc cgctcaggta gccacactgt acaggcagcc actctgctat
     1201 ggctcaataa aatgcattaa agcaa
//
