LOCUS       NM_001395670            1087 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus thiopurine S-methyltransferase (Tpmt), mRNA.
ACCESSION   NM_001395670 XM_006253770
VERSION     NM_001395670.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Chen,D., Lian,F., Yuan,S., Wang,Y., Zhan,Z., Ye,Y., Qiu,Q., Xu,H.,
            Liang,L. and Yang,X.
  TITLE     Association of thiopurine methyltransferase status with
            azathioprine side effects in Chinese patients with systemic lupus
            erythematosus
  JOURNAL   Clin Rheumatol 33 (4), 499-503 (2014)
   PUBMED   24322830
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Liang,J.J., Geske,J.R., Boilson,B.A., Frantz,R.P., Edwards,B.S.,
            Kushwaha,S.S., Kremers,W.K., Weinshilboum,R.M. and Pereira,N.L.
  TITLE     TPMT genetic variants are associated with increased rejection with
            azathioprine use in heart transplantation
  JOURNAL   Pharmacogenet Genomics 23 (12), 658-665 (2013)
   PUBMED   24121523
REFERENCE   3  (bases 1 to 1087)
  AUTHORS   Ayesh,B.M., Harb,W.M. and Abed,A.A.
  TITLE     Thiopurine methyltransferase genotyping in Palestinian childhood
            acute lymphoblastic leukemia patients
  JOURNAL   BMC Hematol 13 (1), 3 (2013)
   PUBMED   24499706
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1087)
  AUTHORS   Dorababu,P., Nagesh,N., Linga,V.G., Gundeti,S., Kutala,V.K.,
            Reddanna,P. and Digumarti,R.
  TITLE     Epistatic interactions between thiopurine methyltransferase (TPMT)
            and inosine triphosphate pyrophosphatase (ITPA) variations
            determine 6-mercaptopurine toxicity in Indian children with acute
            lymphoblastic leukemia
  JOURNAL   Eur J Clin Pharmacol 68 (4), 379-387 (2012)
   PUBMED   22009189
REFERENCE   5  (bases 1 to 1087)
  AUTHORS   Kim,J.H., Cheon,J.H., Hong,S.S., Eun,C.S., Byeon,J.S., Hong,S.Y.,
            Kim,B.Y., Kwon,S.H., Kim,S.W., Han,D.S., Yang,S.K. and Kim,W.H.
  TITLE     Influences of thiopurine methyltransferase genotype and activity on
            thiopurine-induced leukopenia in Korean patients with inflammatory
            bowel disease: a retrospective cohort study
  JOURNAL   J Clin Gastroenterol 44 (10), e242-e248 (2010)
   PUBMED   20308917
REFERENCE   6  (bases 1 to 1087)
  AUTHORS   Lee,D.H. and Kim,D.H.
  TITLE     S-methylation of 2-mercaptopyrazine in rat liver microsomes and
            cytosol
  JOURNAL   Xenobiotica 29 (9), 909-916 (1999)
   PUBMED   10548451
REFERENCE   7  (bases 1 to 1087)
  AUTHORS   Fessing,M.Y., Belkov,V.M., Krynetski,E.Y. and Evans,W.E.
  TITLE     Molecular cloning and functional characterization of the cDNA
            encoding the murine thiopurine S-methyltransferase (TPMT)
  JOURNAL   FEBS Lett 424 (3), 143-145 (1998)
   PUBMED   9539138
REFERENCE   8  (bases 1 to 1087)
  AUTHORS   Hernandez,J.S., Van Loon,J.A., Otterness,D.M., Guerciolini,R. and
            Weinshilboum,R.M.
  TITLE     Thiopurine methyltransferase regulation in rat kidney:
            immunoprecipitation studies
  JOURNAL   Xenobiotica 21 (4), 451-459 (1991)
   PUBMED   1897245
REFERENCE   9  (bases 1 to 1087)
  AUTHORS   Loo,G. and Smith,J.T.
  TITLE     Effect of S-adenosylhomocysteine on sulfhydryl xenobiotic
            transmethylases in rat liver
  JOURNAL   Biochem Biophys Res Commun 128 (2), 965-971 (1985)
   PUBMED   3994729
REFERENCE   10 (bases 1 to 1087)
  AUTHORS   Weinshilboum,R.M., Raymond,F.A. and Pazmino,P.A.
  TITLE     Human erythrocyte thiopurine methyltransferase: radiochemical
            microassay and biochemical properties
  JOURNAL   Clin Chim Acta 85 (3), 323-333 (1978)
   PUBMED   657528
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Jul 30, 2021 this sequence version replaced XM_006253770.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC158556.1, FM129728.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                JAXUCZ010000017.1  17850426-17850524
            100-268             JAXUCZ010000017.1  17852943-17853111
            269-361             JAXUCZ010000017.1  17853785-17853877
            362-494             JAXUCZ010000017.1  17858539-17858671
            495-547             JAXUCZ010000017.1  17859534-17859586
            548-622             JAXUCZ010000017.1  17862470-17862544
            623-708             JAXUCZ010000017.1  17864498-17864583
            709-753             JAXUCZ010000017.1  17866639-17866683
            754-1087            JAXUCZ010000017.1  17868643-17868976
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     gene            1..1087
                     /gene="Tpmt"
                     /note="thiopurine S-methyltransferase"
                     /db_xref="GeneID:690050"
                     /db_xref="RGD:1585162"
     exon            1..99
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            100..268
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..866
                     /gene="Tpmt"
                     /EC_number="2.1.1.67"
                     /note="thiopurine methyltransferase"
                     /codon_start=1
                     /product="thiopurine S-methyltransferase"
                     /protein_id="NP_001382599.1"
                     /db_xref="GeneID:690050"
                     /db_xref="RGD:1585162"
                     /translation="MSLGIKEHPDAEVQKNRVLTLDDWQDKWVTRHIAFHQEQGHQLL
                     KKHFDTFLKGQSGLRVFFPLCGKAIEMKWFADRGHTVVGVEISEIGIREFFAEQNLSY
                     TEEPLTEIAGAKVFKSSSGNISLYCCSIFDLPRANIGKFDRIWDRGALVAVNPGDRDR
                     YADIILSLLRKGYHYLLVVLSYDPTKHTGPPFYVPDAELKKLFGTKCNMQCLEEVDAL
                     EERHKTWGVDYFFEKLYLLTEK"
     exon            269..361
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            362..494
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            495..547
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            548..622
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            623..708
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            709..753
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
     exon            754..1087
                     /gene="Tpmt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggccttcg cccttggggc ggggtctgtc cgtccttgca cgttaccggt ctctcaaagg
       61 cgcgctagag tctgaggcac gcagctgggt gcagctgagg caccgggaga caccaacctc
      121 ggagacaagc cacggatggc accatgtcac tcggtatcaa agagcaccct gatgctgagg
      181 tacaaaagaa ccgggtacta accctggacg actggcaaga caagtgggtg acccgtcaca
      241 tcgcattcca tcaggagcaa gggcatcagc tattaaagaa gcatttcgat acatttctta
      301 aagggcagag tggactgaga gtgtttttcc cgctctgtgg aaaagccatt gagatgaaat
      361 ggttcgcaga ccggggccac actgtagttg gtgtagaaat cagtgaaatt gggattcggg
      421 aattttttgc agaacagaat ctgtcataca cagaagaacc gctcaccgaa attgctggtg
      481 ccaaagtgtt taagagttct tccgggaaca tttccttata ctgttgcagc attttcgacc
      541 ttcccagagc aaacatcggc aagtttgaca ggatttggga tagaggagca ttggtggctg
      601 tcaatccagg cgatcgtgac cgctatgcag atataatact gtccctgctg agaaaagggt
      661 atcactacct cctggttgtc ctttcttatg atccaacaaa acacacaggc ccgccatttt
      721 atgttccaga tgctgaactt aaaaagttat ttggtacaaa atgcaacatg caatgccttg
      781 aggaggtgga tgctcttgaa gaaaggcata aaacctgggg agtcgactac tttttcgaaa
      841 aattgtatct ccttactgag aagtaggaaa aacaaaaaca aaaacaaaac caggccagta
      901 ccggagcaca ccttttatcc cagcaggcta gcctggtcta cagagtgagt tccaagacag
      961 ccaggataca cagagaaacc ttgtctagaa aaaccaaata gaaaaccaac caaccaactt
     1021 aaccaaaaaa cacccagtca atccctgtaa gagtttctga acgattaaaa attctgttga
     1081 aggttaa
//
