LOCUS       NM_001134994            3331 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus thiamin pyrophosphokinase 1 (Tpk1), mRNA.
ACCESSION   NM_001134994 XM_001058258 XM_001073146
VERSION     NM_001134994.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Fragoza R, Das J, Wierbowski SD, Liang J, Tran TN, Liang S, Beltran
            JF, Rivera-Erick CA, Ye K, Wang TY, Yao L, Mort M, Stenson PD,
            Cooper DN, Wei X, Keinan A, Schimenti JC, Clark AG and Yu H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Chen S, Fragoza R, Klei L, Liu Y, Wang J, Roeder K, Devlin B and Yu
            H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   3  (bases 1 to 3331)
  AUTHORS   Wei X, Das J, Fragoza R, Liang J, Bastos de Oliveira FM, Lee HR,
            Wang X, Mort M, Stenson PD, Cooper DN, Lipkin SM, Smolka MB and Yu
            H.
  TITLE     A massively parallel pipeline to clone DNA variants and examine
            molecular phenotypes of human disease mutations
  JOURNAL   PLoS Genet 10 (12), e1004819 (2014)
   PUBMED   25502805
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3331)
  AUTHORS   Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M,
            Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail
            S, Li P and Sutton G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
REFERENCE   5  (bases 1 to 3331)
  AUTHORS   Nosaka K, Onozuka M, Nishino H, Nishimura H, Kawasaki Y and Ueyama
            H.
  TITLE     Molecular cloning and expression of a mouse thiamin
            pyrophosphokinase cDNA
  JOURNAL   J Biol Chem 274 (48), 34129-34133 (1999)
   PUBMED   10567383
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473959.1.
            
            On or before Sep 17, 2008 this sequence version replaced
            XM_001073146.1, XM_001058258.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132262, SAMD00132265
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q24"
     gene            1..3331
                     /gene="Tpk1"
                     /note="thiamin pyrophosphokinase 1"
                     /db_xref="GeneID:680668"
                     /db_xref="RGD:1589408"
     exon            1..145
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            146..203
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..892
                     /gene="Tpk1"
                     /EC_number="2.7.6.2"
                     /note="thiamine pyrophosphokinase"
                     /codon_start=1
                     /product="thiamin pyrophosphokinase 1"
                     /protein_id="NP_001128466.1"
                     /db_xref="GeneID:680668"
                     /db_xref="RGD:1589408"
                     /translation="MEHVFTPLEPLLPTGDLKFCLVVLNQTLDPHFRHLWRKALLRAC
                     ADGGANHLYDLTEGERESFLPEFINGDFDSIRPEVKEYYTKKGCDLISTPDQDHTDFT
                     KCLQVLQRKIEEKELQVDVIVTLGGLGGRFDQIMASVNTLFQATDIIPVPIIIIQKES
                     LIYLLQPGKHRLRVDTGMEGSWCGLIPVGQPCNHVTTTGLKWNLTNDVLGFGTLVSTS
                     NTYDGSGLVTVETDHPLLWTMAIKT"
     exon            204..275
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            276..345
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            346..418
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            419..514
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            515..661
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..773
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            774..3331
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaaggggcgg ggctctgcgc tcgcgtacag ggccttctcc cggggactcg cagcacgcca
       61 caggccccgc agccgagcgc cgagctgccg atcagtcatt cctgcagccc tcgctcgggt
      121 cgctcgtggc tccagccgcc attgtaaccc ataccccgct atggagcatg tctttacccc
      181 gctggagccc ctgctaccca cgggagactt gaaattctgc cttgtggttc ttaatcagac
      241 tttggatcca cattttcggc atctttggag aaaagctctc ttaagagcct gtgctgatgg
      301 aggtgccaat cacttatatg acctcactga aggagaaaga gaaagctttt tgcctgaatt
      361 catcaatggg gactttgatt ctattaggcc tgaagtcaaa gagtactaca ccaagaaggg
      421 ctgtgatctt atttccactc ctgaccaaga ccacactgac tttaccaagt gtcttcaagt
      481 gctccaaagg aagatagaag aaaaagagct gcaggttgac gtgatcgtga ccctgggagg
      541 tctcggtggg cgttttgacc agatcatggc atctgtgaat actcttttcc aagccactga
      601 catcattcct gtgccaatta taataatcca aaaggaatct ctcatctacc tcctccaacc
      661 aggaaagcac agactccgtg tagacactgg aatggaaggc agctggtgtg gccttattcc
      721 tgttggacag ccttgcaacc atgtgacgac aacaggcctg aaatggaacc tcacaaatga
      781 tgtgctcggc tttggaaccc tggtcagtac ttccaacacc tacgatgggt ccggccttgt
      841 cactgtggaa actgaccatc cgctcctctg gaccatggcc atcaagacct aacccacagt
      901 gtagcatcca tcttgtgcct gtgccttccc ggacctgccc actggttcat tggtcaacat
      961 gaacaatcac ataggtgtgc agaagtctaa ccgtcttcgt agggagccat gagctttcaa
     1021 ggggtgaagg catagctgag cagcagagca cttgtgcatg cacaaggcct tgggttcaac
     1081 cctcacacca tgaactagag atgttagggt ttaaagattc caagttatgc tataaatgat
     1141 atctcaatgc tatattgagg gaaaaattat gttcgatcaa agcaaataag ttttatcata
     1201 ctctattgag aaagctaacg tggatcagtt acattatgta attcagtttt gatcatttta
     1261 atttaattaa tcagcccatt tacctttttt tttttatttt gaagcaccag aagctgtttt
     1321 ctgttgcctt ggagtctggc acttccttca gtgcctgaca accagggctg cctcctcctg
     1381 agtgtctcct aacacggact ccactctgta atgcacatta tctcctggga cgctgagata
     1441 gaatggtatc agttcatttt gacctcatct tttaaaagat aaaaataatt gatgtaattg
     1501 ttaggtttga gctttagaga tgcaggcatc ctaatgaaaa ttgtgacaca tccaaaagct
     1561 ttgtgctgag caatagatca taagcctgaa aattcagcca cttttaacgt atttcacact
     1621 agaggatgca gagatggctc agtggttagg ggcactgatt gctcttccag agatcctgat
     1681 ttcagttccc agcaaccaca tgggggctca caaccatctg taatgagatc cgatgccctc
     1741 ttctggcaac aggtgtacat acagatagag tactcagaca taaaaataaa taaatcttgg
     1801 gggaaaacat acttcacatt aaaaaacaaa atattaatcc atggaccata ttttctgaca
     1861 tcaccagaaa attagtgtgc tatggagata atgaacctgc caaggaattg acgcctgaag
     1921 cctgtttctg catcccagtc cactgcgtgc ctgcttcctg aaccccttct ctctgctgtg
     1981 tgctgggaag ggttgtggtc actgagtgtg aggaagaagc acatgtataa actctaactg
     2041 ctaacctgtt gcaggtgtag agcggataat gcagttcctg acctagccta agcttgcggt
     2101 gcattcagat ttattcaggt ttctctcatg agcgccccta ctcatcttcc caccctctcc
     2161 tatcccattt gttaaactgc atcttgccaa acaaaccatg atttaaggca acctgtgtac
     2221 catgtttcat ttatccaatg actgtgttac tgtctacttg gtgctacaac agtatgaagc
     2281 ttaaaacata aatggaggaa gaaaagggat cagttgaacc aataccaagt tcttaacaga
     2341 aatagttaga atgattgtta tcattttatg caaatatatg attccgaaat ctgaatgtca
     2401 aataaaaagt tatccatgtg gatgtctgta gtgggtttct cagagaatgt ttaatgttat
     2461 cccttgtact tcaatatgac taatctctca taatcggtgt ttctgttaag atgtgtaata
     2521 cataattaaa gtctcagatt ggatgtcaga tagcaatcca atagtattat gttcttcctt
     2581 gaatcttttc ccatggacat agagaccagg tcatctctag acaacctgga tgggtgattt
     2641 atatttcatc gtttcagact ccgcctgctc tagctataaa gagaagaatc tcatgtctta
     2701 ccttacttac cattcctcac tttgtaggaa ttagaaaaga gacaaataga gatagtttgc
     2761 attctgggtg tctagatcct ggaggaaagg agcgagtaga gagagctaca tatcttctgg
     2821 ggagagaaga taaagagaac tggtagagga agacgtctga gaagcaggaa gagacggagt
     2881 cagacagttg aagagcacat tgatgacagg cccatgacta ggatcctctg catcttctca
     2941 atacaaatac agaccttaga ggccccaagt atctcttcca ttcccaacag acagatgcca
     3001 gtctgccagg agagaggagc agccaagatc ttcactgact catttaactc agaacttcca
     3061 gcaggaaagc ctgtgcaaac atctagttgc tgtctcaaac aatgtgtgca cacatgaaat
     3121 taggagggaa aggagatatg gccagagttg aggtgcgcag ccttctcctt taccagcact
     3181 ctttactact gcacagtagg gctcagccat gtctctagca gactgctgct gccgatctgt
     3241 ccataactac aagcacattc tgtacatgcc aaagacatat gtccatcatt caaaaaactt
     3301 actgtcaaag aataatgtct tgtttaacct g
//
