LOCUS       NM_001399445            2883 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus two pore segment channel 2 (Tpcn2), transcript
            variant 1, mRNA.
ACCESSION   NM_001399445 XM_006230784
VERSION     NM_001399445.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Hu,W., Zhao,F., Chen,L., Ni,J. and Jiang,Y.
  TITLE     NAADP-induced intracellular calcium ion is mediated by the TPCs
            (two-pore channels) in hypoxia-induced pulmonary arterial
            hypertension
  JOURNAL   J Cell Mol Med 25 (15), 7485-7499 (2021)
   PUBMED   34263977
  REMARK    GeneRIF: NAADP-induced intracellular calcium ion is mediated by the
            TPCs (two-pore channels) in hypoxia-induced pulmonary arterial
            hypertension.
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Ou,X., Liu,Y., Lei,X., Li,P., Mi,D., Ren,L., Guo,L., Guo,R.,
            Chen,T., Hu,J., Xiang,Z., Mu,Z., Chen,X., Chen,J., Hu,K., Jin,Q.,
            Wang,J. and Qian,Z.
  TITLE     Characterization of spike glycoprotein of SARS-CoV-2 on virus entry
            and its immune cross-reactivity with SARS-CoV
  JOURNAL   Nat Commun 11 (1), 1620 (2020)
   PUBMED   32221306
  REMARK    Erratum:[Nat Commun. 2021 Apr 1;12(1):2144. PMID: 33795662]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2883)
  AUTHORS   Hockey,L.N., Kilpatrick,B.S., Eden,E.R., Lin-Moshier,Y.,
            Brailoiu,G.C., Brailoiu,E., Futter,C.E., Schapira,A.H.,
            Marchant,J.S. and Patel,S.
  TITLE     Dysregulation of lysosomal morphology by pathogenic LRRK2 is
            corrected by TPC2 inhibition
  JOURNAL   J Cell Sci 128 (2), 232-238 (2015)
   PUBMED   25416817
REFERENCE   4  (bases 1 to 2883)
  AUTHORS   Tsaih,S.W., Holl,K., Jia,S., Kaldunski,M., Tschannen,M., He,H.,
            Andrae,J.W., Li,S.H., Stoddard,A., Wiederhold,A., Parrington,J.,
            Ruas da Silva,M., Galione,A., Meigs,J., Hoffmann,R.G., Simpson,P.,
            Jacob,H., Hessner,M. and Solberg Woods,L.C.
  CONSRTM   Meta-Analyses of Glucose and Insulin-Related Traits Consortium
            (MAGIC) Investigators
  TITLE     Identification of a novel gene for diabetic traits in rats, mice,
            and humans
  JOURNAL   Genetics 198 (1), 17-29 (2014)
   PUBMED   25236446
  REMARK    GeneRIF: Tpcn2 was identified as a novel gene for diabetic traits
            in rats.
REFERENCE   5  (bases 1 to 2883)
  AUTHORS   Buga,A.M., Scholz,C.J., Kumar,S., Herndon,J.G., Alexandru,D.,
            Cojocaru,G.R., Dandekar,T. and Popa-Wagner,A.
  TITLE     Identification of new therapeutic targets by genome-wide analysis
            of gene expression in the ipsilateral cortex of aged rats after
            stroke
  JOURNAL   PLoS One 7 (12), e50985 (2012)
   PUBMED   23251410
REFERENCE   6  (bases 1 to 2883)
  AUTHORS   Chen,G., Yang,Y., Frohlich,O., Klein,J.D. and Sands,J.M.
  TITLE     Suppression subtractive hybridization analysis of low-protein diet-
            and vitamin D-induced gene expression from rat kidney inner
            medullary base
  JOURNAL   Physiol Genomics 41 (3), 203-211 (2010)
   PUBMED   20197420
REFERENCE   7  (bases 1 to 2883)
  AUTHORS   Zhu,M.X., Ma,J., Parrington,J., Calcraft,P.J., Galione,A. and
            Evans,A.M.
  TITLE     Calcium signaling via two-pore channels: local or global, that is
            the question
  JOURNAL   Am J Physiol Cell Physiol 298 (3), C430-C441 (2010)
   PUBMED   20018950
  REMARK    Review article
REFERENCE   8  (bases 1 to 2883)
  AUTHORS   Zong,X., Schieder,M., Cuny,H., Fenske,S., Gruner,C., Rotzer,K.,
            Griesbeck,O., Harz,H., Biel,M. and Wahl-Schott,C.
  TITLE     The two-pore channel TPCN2 mediates NAADP-dependent Ca(2+)-release
            from lysosomal stores
  JOURNAL   Pflugers Arch 458 (5), 891-899 (2009)
   PUBMED   19557428
REFERENCE   9  (bases 1 to 2883)
  AUTHORS   Brailoiu,E., Churamani,D., Cai,X., Schrlau,M.G., Brailoiu,G.C.,
            Gao,X., Hooper,R., Boulware,M.J., Dun,N.J., Marchant,J.S. and
            Patel,S.
  TITLE     Essential requirement for two-pore channel 1 in NAADP-mediated
            calcium signaling
  JOURNAL   J Cell Biol 186 (2), 201-209 (2009)
   PUBMED   19620632
REFERENCE   10 (bases 1 to 2883)
  AUTHORS   Calcraft,P.J., Ruas,M., Pan,Z., Cheng,X., Arredouani,A., Hao,X.,
            Tang,J., Rietdorf,K., Teboul,L., Chuang,K.T., Lin,P., Xiao,R.,
            Wang,C., Zhu,Y., Lin,Y., Wyatt,C.N., Parrington,J., Ma,J.,
            Evans,A.M., Galione,A. and Zhu,M.X.
  TITLE     NAADP mobilizes calcium from acidic organelles through two-pore
            channels
  JOURNAL   Nature 459 (7246), 596-600 (2009)
   PUBMED   19387438
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Jan 7, 2022 this sequence version replaced XM_006230784.4.
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582424.393613.1,
                                           SRR22582419.1239494.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                JAXUCZ010000001.1  209875381-209875457 c
            78-136              JAXUCZ010000001.1  209871312-209871370 c
            137-213             JAXUCZ010000001.1  209870397-209870473 c
            214-391             JAXUCZ010000001.1  209869851-209870028 c
            392-508             JAXUCZ010000001.1  209868330-209868446 c
            509-615             JAXUCZ010000001.1  209865133-209865239 c
            616-691             JAXUCZ010000001.1  209863629-209863704 c
            692-791             JAXUCZ010000001.1  209861956-209862055 c
            792-857             JAXUCZ010000001.1  209861127-209861192 c
            858-922             JAXUCZ010000001.1  209860123-209860187 c
            923-1023            JAXUCZ010000001.1  209859134-209859234 c
            1024-1105           JAXUCZ010000001.1  209858486-209858567 c
            1106-1192           JAXUCZ010000001.1  209858257-209858343 c
            1193-1312           JAXUCZ010000001.1  209853920-209854039 c
            1313-1372           JAXUCZ010000001.1  209853718-209853777 c
            1373-1501           JAXUCZ010000001.1  209852568-209852696 c
            1502-1551           JAXUCZ010000001.1  209851815-209851864 c
            1552-1651           JAXUCZ010000001.1  209850316-209850415 c
            1652-1723           JAXUCZ010000001.1  209849634-209849705 c
            1724-1797           JAXUCZ010000001.1  209848983-209849056 c
            1798-1879           JAXUCZ010000001.1  209848539-209848620 c
            1880-1962           JAXUCZ010000001.1  209848330-209848412 c
            1963-2044           JAXUCZ010000001.1  209847746-209847827 c
            2045-2139           JAXUCZ010000001.1  209847330-209847424 c
            2140-2883           JAXUCZ010000001.1  209845827-209846570 c
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q42"
     gene            1..2883
                     /gene="Tpcn2"
                     /note="two pore segment channel 2"
                     /db_xref="GeneID:309139"
                     /db_xref="RGD:1311779"
     exon            1..77
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..2218
                     /gene="Tpcn2"
                     /note="two pore calcium channel protein 2"
                     /codon_start=1
                     /product="two pore channel protein 2"
                     /protein_id="NP_001386374.1"
                     /db_xref="GeneID:309139"
                     /db_xref="RGD:1311779"
                     /translation="MAAEAQPLLGRDRGGGQAHSGADQELCIDQAVVFIEDAIKYRSI
                     YHRLDAGSLWLYRWYYSNVCQRVLGFTIFLILALAFVEVPSSFTRTADVRYRSQPWQP
                     PCGLTETIEALCLLVFLADLSVKGYLVGQAQLQQNLWLLAYFVVLVVSVVDWIVSLSL
                     NCEESLRVRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLTIHLCLFTVIGML
                     LFTIAEKDEAQNKERLTYFRNLPEALTSLLVLLTTSNNPDVMIPAYTQNRAYALFFII
                     FTLIGSLFLMNLLTAIIYNQFRGYLMKSLQTSLFRRRLGARAAYEVLTLQAGPNGTTS
                     ELVGVNPENFLRVLQKTQLNKIHKQAIIQKVHSYEGRPILADEFQKLFDEVDKGIVKE
                     RPPKPQYQSPFLQTAQFIFSHRYFDYLGNLIALGNLLSICVFLVLDSNLLPGERNDFV
                     LGILNYIFILYYLLELLFKVFALGLRGYLFYHSNVFDGLLTIILLVLEISTLAVYRLP
                     HPGWKPEQYGPLSLWDMTRLVNILIVIRFLRIIPSIKPTAVVASTILGLIQNLRAFGG
                     ILVVVYYVFAMIGINLFRGVIVPPGNSSLVPDNSSAPCGSFEQLGYWPNNFDDFAAAL
                     ITLWDVMVVNNWQVILEAYKHYSGPWSMVYFVLWWLVSSVIWINLFLALLLENFLHRW
                     DPQGHKQLLVGTKQITYEMSVELMFRDILEEPKEEELMEKLHKHPHLHLCR"
     exon            78..136
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            137..213
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            214..391
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            392..508
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            509..615
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            616..691
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            692..791
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            792..857
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..922
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1023
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1105
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1192
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1312
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1372
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1501
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1551
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1651
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1723
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1797
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1879
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..1962
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2044
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2139
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2883
                     /gene="Tpcn2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggtgataga ggtggtatgg cggcagaagc gcagcccctt ctgggccggg accgaggtgg
       61 tggccaggcc cactctggcg ctgatcagga gctctgcata gaccaggctg tggtcttcat
      121 tgaagatgcc ataaagtacc gatccatcta ccaccgcctg gacgctggct cactgtggct
      181 ttaccgctgg tattactcga acgtgtgcca acgagtgctg ggcttcacca ttttcctgat
      241 cctggccttg gcttttgtgg aggtcccatc ttccttcaca aggactgcgg atgtgcgcta
      301 ccgctcccag ccctggcagc caccctgtgg cctgacagag acaattgagg cgctctgctt
      361 gctggtcttc ctggctgacc tctctgtgaa gggctacctg gtggggcagg cccagttgca
      421 gcagaacctg tggctgctgg cttactttgt ggtgctggtt gtgtctgtgg tggactggat
      481 cgtttcgctg agcctcaact gtgaggagtc tctgcgggtg cgccggctac tccgaccctt
      541 cttcctgctg cagaattcct ccatgatgaa gaagaccctg aaatgtatac ggtggtcact
      601 accagaaatg gctagtgttg ggctgctgct gactatccac ctgtgtctct tcaccgtaat
      661 cgggatgctg ctgttcacca ttgctgagaa ggatgaagca cagaacaagg agaggctgac
      721 ctacttccgg aaccttccag aagcactgac ctcactccta gtgctgctga ctacttccaa
      781 caaccctgat gtgatgatcc ctgcgtatac ccagaaccgg gcctatgctc tcttcttcat
      841 aatcttcacc ttgataggaa gcttgtttct gatgaacctg ctgacggcca tcatctataa
      901 tcagttccga ggctacctaa tgaaatctct acagacttca ctgttccggc ggcgactagg
      961 ggcccgtgcg gcctatgaag tcctcaccct tcaggcaggg ccaaatggaa ccacctctga
     1021 gttagtcggg gtaaatcccg agaacttcct gcgagtgctt cagaaaaccc agctgaacaa
     1081 aatccacaaa caagccatca tacagaaggt gcactcctat gaaggccgcc cgatcctggc
     1141 cgatgagttc cagaaactct ttgatgaggt tgacaaaggt atagtcaaag agcgcccgcc
     1201 gaagccccag taccagtctc cgtttctgca gactgcccag ttcatcttca gccaccgcta
     1261 ctttgactac ctggggaacc tcattgctct gggaaacctc ttgtctattt gtgtgttcct
     1321 ggtgctggac tcaaacctgc tgcctgggga acggaacgac tttgtcctgg ggattctcaa
     1381 ctacattttc atcttgtact acctgctgga gctgctgttc aaggtgttcg cgctgggcct
     1441 acggggctac ctgttctacc acagcaacgt gttcgatggc ctcctgacca tcatcctgct
     1501 ggttttggag atttctactc tggccgtgta ccgattgccg cacccaggat ggaagccaga
     1561 gcagtatggc cccctgtcgc tttgggacat gactcggctg gtgaacattc tgattgtgat
     1621 acgcttcctg cgcatcatcc ccagcataaa gccaacggct gtggtagcca gcaccatcct
     1681 gggcctaatc cagaacctgc gggcatttgg tgggatcctg gtggtggtat actatgtgtt
     1741 cgccatgatt gggatcaacc tgttccgagg tgtcattgtg cctcccggaa acagcagcct
     1801 ggttcctgat aacagctcag ctccgtgtgg aagctttgag cagctaggtt actggcccaa
     1861 caacttcgac gactttgctg ctgctctgat cacactgtgg gacgtgatgg tggtgaacaa
     1921 ttggcaggtg atcctggaag cctataagca ctactccggc ccgtggtcga tggtgtattt
     1981 tgtgctgtgg tggttggtgt cctctgtcat ctggatcaac ctgtttctgg ctctgcttct
     2041 ggagaacttt ctccacagat gggaccctca aggtcataag cagctcctgg ttgggaccaa
     2101 gcagatcacc tacgagatgt ctgtggagct catgttcaga gatatcctag aagagcccaa
     2161 ggaggaggaa ctgatggaga agctacacaa gcatccacac ttgcatctgt gcaggtgaca
     2221 tcagagtggc ctctagctgg ggcgacagga cagagatgct ggcgtggccc aggctgtgga
     2281 gacagcagcc ggtcgagatt gagcatgaaa agatggtacc tgggacagac cactcaagga
     2341 tagacagagg ctggaataag gactcctctc ctgcagctgc tcagcagtag ccaccgtgca
     2401 atctctgtag ccgtgacaga ctggtggtca gcactacagg acggccttct tccatttgct
     2461 gttcttcaga gctgcctggg tgactcacta aggagagaga tttccaagca tcctcaaagc
     2521 caggaatgga gaaggcctcg gctgtctttt acccacaagc tcatctgtgc cttcagcgtc
     2581 agtggcattt gtgaactcaa gcctgcccag gacagtagct ggcaggtttc cagggcatca
     2641 gggtcatgtg tagggaatgt cacctctttg tttcagtgcc tatggatgtg ataggcgtgt
     2701 cggggggggt gggtcactgt gtcagccatt ttctgttttg taaagaagga gattgtgttt
     2761 attctgacct ctccctctac ctcagggttg ttgggtaggg agagcaccag gttgtaaggt
     2821 gagagctccc acctgtgatg agaagcatct tttaatgaca tgcacaaaga cttgtaagcc
     2881 caa
//
