LOCUS       NM_139332               4735 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus two pore segment channel 1 (Tpcn1), mRNA.
ACCESSION   NM_139332
VERSION     NM_139332.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Hu,W., Zhao,F., Chen,L., Ni,J. and Jiang,Y.
  TITLE     NAADP-induced intracellular calcium ion is mediated by the TPCs
            (two-pore channels) in hypoxia-induced pulmonary arterial
            hypertension
  JOURNAL   J Cell Mol Med 25 (15), 7485-7499 (2021)
   PUBMED   34263977
  REMARK    GeneRIF: NAADP-induced intracellular calcium ion is mediated by the
            TPCs (two-pore channels) in hypoxia-induced pulmonary arterial
            hypertension.
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Trufanov,S.K., Rybakova,E.Y., Avdonin,P.P., Tsitrina,A.A.,
            Zharkikh,I.L., Goncharov,N.V., Jenkins,R.O. and Avdonin,P.V.
  TITLE     The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i
            Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction
  JOURNAL   Cells 8 (10), 1144 (2019)
   PUBMED   31557916
  REMARK    GeneRIF: TPC1 is involved in the norepinephrine (NE)-stimulated
            [Ca(2+)]i rise in Smooth muscle cells. Inhibition of TPC1 activity
            by NED 19 could be the reason for partial inhibition of aortic
            rings contraction in response to NE.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4735)
  AUTHORS   She,J., Guo,J., Chen,Q., Zeng,W., Jiang,Y. and Bai,X.C.
  TITLE     Structural insights into the voltage and phospholipid activation of
            the mammalian TPC1 channel
  JOURNAL   Nature 556 (7699), 130-134 (2018)
   PUBMED   29562233
REFERENCE   4  (bases 1 to 4735)
  AUTHORS   Gomez-Suaga,P., Luzon-Toro,B., Churamani,D., Zhang,L.,
            Bloor-Young,D., Patel,S., Woodman,P.G., Churchill,G.C. and
            Hilfiker,S.
  TITLE     Leucine-rich repeat kinase 2 regulates autophagy through a
            calcium-dependent pathway involving NAADP
  JOURNAL   Hum Mol Genet 21 (3), 511-525 (2012)
   PUBMED   22012985
REFERENCE   5  (bases 1 to 4735)
  AUTHORS   Rietdorf,K., Funnell,T.M., Ruas,M., Heinemann,J., Parrington,J. and
            Galione,A.
  TITLE     Two-pore channels form homo- and heterodimers
  JOURNAL   J Biol Chem 286 (43), 37058-37062 (2011)
   PUBMED   21903581
REFERENCE   6  (bases 1 to 4735)
  AUTHORS   Zhu,M.X., Ma,J., Parrington,J., Calcraft,P.J., Galione,A. and
            Evans,A.M.
  TITLE     Calcium signaling via two-pore channels: local or global, that is
            the question
  JOURNAL   Am J Physiol Cell Physiol 298 (3), C430-C441 (2010)
   PUBMED   20018950
  REMARK    Review article
REFERENCE   7  (bases 1 to 4735)
  AUTHORS   Brailoiu,E., Churamani,D., Cai,X., Schrlau,M.G., Brailoiu,G.C.,
            Gao,X., Hooper,R., Boulware,M.J., Dun,N.J., Marchant,J.S. and
            Patel,S.
  TITLE     Essential requirement for two-pore channel 1 in NAADP-mediated
            calcium signaling
  JOURNAL   J Cell Biol 186 (2), 201-209 (2009)
   PUBMED   19620632
REFERENCE   8  (bases 1 to 4735)
  AUTHORS   Kato,M., Tanaka,N., Usui,S. and Sakuma,Y.
  TITLE     The SK channel blocker apamin inhibits slow afterhyperpolarization
            currents in rat gonadotropin-releasing hormone neurones
  JOURNAL   J Physiol 574 (Pt 2), 431-442 (2006)
   PUBMED   16627563
  REMARK    GeneRIF: Rat GnRH neurones express all five subtypes of
            voltage-gated Ca(2+) channels and exhibit an apamin-sensitive
            I(sAHP), which may allow continuous firing in response to a
            relatively strong depolarizing input.
REFERENCE   9  (bases 1 to 4735)
  AUTHORS   Ishibashi,K., Suzuki,M. and Imai,M.
  TITLE     Molecular cloning of a novel form (two-repeat) protein related to
            voltage-gated sodium and calcium channels
  JOURNAL   Biochem Biophys Res Commun 270 (2), 370-376 (2000)
   PUBMED   10753632
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC062072.1, AB018253.2, CK473123.1, CA339864.1 and
            JAXUCZ010000012.1.
            
            On Sep 15, 2005 this sequence version replaced NM_139332.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062072.1, AB018253.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1404              BC062072.1         1-1404
            1405-1795           AB018253.2         1408-1798
            1796-2430           BC062072.1         1796-2430
            2431-3068           CK473123.1         9-646
            3069-3388           CA339864.1         307-626
            3389-4735           JAXUCZ010000012.1  41688854-41690200
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..4735
                     /gene="Tpcn1"
                     /note="two pore segment channel 1"
                     /db_xref="GeneID:246215"
                     /db_xref="RGD:708494"
     exon            1..121
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            122..365
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..2707
                     /gene="Tpcn1"
                     /note="voltage-dependent calcium channel protein TPC1;
                     voltage-gated Ca channel; two pore channel 1"
                     /codon_start=1
                     /product="two pore calcium channel protein 1"
                     /protein_id="NP_647548.2"
                     /db_xref="GeneID:246215"
                     /db_xref="RGD:708494"
                     /translation="MSVILDDDVLLILTLDEELSAPLTPSNGLGQEDLPSKNGGGQSG
                     PNSQVPSLVSGADSPPSSPPGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYL
                     FVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYVHATLELFALMVVVFELCMKL
                     RWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGG
                     VRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFV
                     LLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKS
                     LLLHKRTAIQHAYHLLVSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNT
                     PLLSLKDFYDIYEVAALQWKAKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYL
                     VVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGW
                     NLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLP
                     RMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEG
                     YYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTI
                     IVAFILEAFVFRMNYSRKSQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDITR
                     LLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQQLRGSA
                     PSPAAQQTPGSRQRSQTVT"
     exon            366..493
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            494..670
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            671..784
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            785..915
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1004
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1039
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1098
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1198
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1315
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1440
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1489
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1553
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1598
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1655
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1709
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1782
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1890
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..1962
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2052
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2168
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2215
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2284
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2369
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2509
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2590
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..4735
                     /gene="Tpcn1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcggtgag gcggcggtgg cggctgctga ggctccgctg tggggatatt ggcggcagca
       61 gctgcgggct gagctgcgct gtgcagaccc ggtgcacggt gcgggatccg ggacggcgcg
      121 tcccctagaa gatgcccctg atggaacggg ctctgggaag cttctctggc cccgtggctt
      181 tgaacaggag ctcaagccgg aggccattta aagccctggc tgttgtatcc tgaggaccac
      241 aggtcaggag aagatgtctg taattttaga cgacgacgtg ctgctcatcc tgaccttgga
      301 cgaggagctg agtgcgccgc tgactccttc caacggcctg ggccaagaag acctgcccag
      361 caaaaatggg ggcggccaaa gcggccccaa ctcccaagtt cccagtctgg tctctggagc
      421 cgacagtccc ccctccagtc cccccggaca caactgggag atgaattatc aagaggcagc
      481 aatctacctc caggaaggcc agaacaatga caagttcttc acccacccca aggatgccag
      541 agcgctggct gcctacctct tcgtacacaa ccacttcttt tatatgatgg agctgctcac
      601 agccctgctt ctgctgctgc tgtcactgtg cgagtcaccc gctgtccctg cgctcaagct
      661 ccgaacttac gtccacgcca ccctggagct gttcgccctc atggtggtgg tatttgaact
      721 ctgcatgaaa ttgcggtggc tgggcttcca cacattcgtc aggcacaaac gcaccatggt
      781 caagacgtct gttttggtgg tgcagttcat cgaggccatt gtggtgctgg tgcggcagac
      841 gtcccacgtg cgggtgaccc gggcactacg ctgcattttc ctggtggact gtcggtactg
      901 tggtggcgtg cggcgcaacc tgcggcagat cttccagtcg ctgccgcctt tcatggacat
      961 cctcctgctg ctgctcttct ttatgatcat cttcgccatc ctcggtttct acttattctc
     1021 tacgaaccct tccgaccctt acttcaacac cctggagaac agcatcgtca acctgtttgt
     1081 tctcctgacc acagccaact ttccagacgt catgatgccc tcctactccc ggaacccctg
     1141 gtcctgtgtc ttctttatcg tgtacctctc cattgagctg tacttcatca tgaacctgct
     1201 cctagccgtg gtgttcgaca ccttcaacga catcgaaaag cacaagttca agtccttgct
     1261 gctgcataag cgaacagcca tccagcacgc ataccacctg ctcgtcagcc aacggaggcc
     1321 ggccggcatc tcctacaggc agtttgaagg cttgatgcgc ttctacaagc cccggatgag
     1381 cgctagggag cgcttcctga ctttcaaggc cctgaatcag agcaacacac ctctgcttag
     1441 cctgaaggac ttctatgata tttatgaagt tgctgctctg cagtggaagg caaagaagaa
     1501 cagacagcac tggtttgacg agcttccccg gacagccttc ctcatcttca aagggattaa
     1561 catccttgtg aattccaagg ccttccagta tttcatgtac ctggtggtgg ctgtcaacgg
     1621 tgtctggatc ctagtggaga cattcatgtt gaaaggtggg aatttcatct caaagcacgt
     1681 gccctggagt tacctcgtgt ttcttaccat ctatggagtt gagctgttca tgaaggtggc
     1741 tggcctgggc cctgtggagt acttgtcctc tggatggaac ctgttcgatt tctcagtcac
     1801 agcatttgcc ttcctgggac tgctcgcgct gacgctcaac atggagccct tctactttat
     1861 tgtggtcctg cgtccccttc agctgctgag gttattcaag ttgaagaaac gctaccgcaa
     1921 tgtgttggac accatgtttg agctgctgcc acgaatggcc agcctcggcc tcacactgct
     1981 caccttctac tactccttcg ccatcgtggg catggagttc ttcagtgggc gactgtcccc
     2041 caactgctgc aactccagca cggtggctga cgcctatcga ttcatcaatc acacggtggg
     2101 caataagacc aaggtggagg aaggctacta ctatctcaat aactttgaca acatcctcaa
     2161 cagctttgtg accttgtttg agctcaccgt tgtcaacaac tggtacatca tcatggaagg
     2221 cgtcacctcg cagacttccc actggagccg cctgtacttc atgacctttt acatcgtgac
     2281 catggtggtg atgaccatta tcgtggcctt catcctggag gccttcgtct tccgcatgaa
     2341 ctacagccgc aagagccagg aatcggaagt ggacagtggc atcgtcatcg agaaggagat
     2401 gtccaaggag gagcttctgg ccatcctgga gcttcatcga gaggcgcgag gcacctcctc
     2461 tgacatcacc cggctgctcg acaccctctc tcagatggag aaataccagc aaaactccat
     2521 ggtgtttctg ggacggcgat cgagaaccaa aagtgacctg agcctgaaga tgtaccagga
     2581 ggagatccag gagtggtacg aggagcatgc ccgggaacag gagcagcagc agctcagggg
     2641 cagcgcgcca agccctgcag cccagcagac ccccggcagt cgccagcgct cccagaccgt
     2701 cacctagccc ggctccgtca agccagcctc ttctatgcaa taacaccata gtattatgta
     2761 ctacgatgta tggaaaccat gtgtgtatgt agacacactc atatatatgc acatatttac
     2821 acagagagag aaaaggacta gccagggtcc gtccgaacca cctcgctctg tcttcctgag
     2881 ctccagaaat tggtttaggc tggaggctca gacctggctg agctcttcct accatggaaa
     2941 attctggaag ccctcctgct caagaaggct gcttaaatag gctgtctcat cttagcaatg
     3001 ccagcattcc ctgggggacc ttgctgtagc ctcgggcttc gtccctgcat tgttgtctac
     3061 ttgtcctcca gccatcacag gtgtctgtgt ctggtattag ggacaaaacc actaggaaga
     3121 aaccccacat cccgctgaat gacgtctgta gatgacaaag tgctagaagg tcccgttgag
     3181 ggaatcagtg cctgggaccc agtgagaaac cggacagagg ccagagatgg ctcagagaca
     3241 gcagagtgta ctcgctcagg cttctagtcc tttcttgttg ctgaaaccct tccacagcct
     3301 cgtagggaag ctcacccagc aggagaaagc caactggaga ccctgggaat ttgactcatt
     3361 caggaagagt tcatttgaac tggtgctgaa ggcaggtccc tcacccccac acctccactc
     3421 ttgaagtaga cactgtgttt ggggagggag ccacacacag cacagggaca ctaactgccc
     3481 atcatagcag tccctggctg gccaccacgc cacggctagc tgcttttcaa ggactctgag
     3541 gcacagtgag tactggcgtc ctccaaggtt ctcataagct aggtgggtag gggaggaggg
     3601 tgaggtgagc actcagcagc cttgtaccgt gccttgtggg ggttcttttg atgtcacggt
     3661 tgggtagtga agtggcagag gcaggcgtag caggggtgga gccctggtca cttcatcctg
     3721 ctgtgttgtc cacaaggggt gggaaggagg cagggctgtc tgaagttgcc aggcagccct
     3781 gtgaacctgc ccttccgaac ctactgctgc ctgggaattg gagtgggcag gcaggcacag
     3841 gtcccctccc gttctcccag ctgctgaacc aggggccaag ccagaaagtg ggtgttttta
     3901 ctctaaataa agcacagtct gccccacacc gcacccccag gaaaaccact ggtttccaca
     3961 gagagtcagg agactggcaa agtcccttcc acctcaaaga gctcttggcc tctacctaaa
     4021 ctcaacctag actctaggcc acagacctga gaagcaactt catctagctg gctgccttag
     4081 cttgtgtatg agtggcattt tggaagcaga aaggggggag gcctatgaag agagcctagg
     4141 gcctctgatt aacccgcctg gttctaggct accccaggta cccttggtct aggggagcag
     4201 gctcaggcag agggactggt cttgggccct gatagaggta ctaaaacccc caggttcagc
     4261 acataccagc caggtgcctg agggagtttg ggctgtcagg ccaaactgag cttgtcctgt
     4321 agcctgagaa aacctgtccc cacagaagtc accttgagca cacaccaagc tgaccctcaa
     4381 acttgctagc ccagcaggga acagactggg tcacccaggg atgtaagctg aggcccctgg
     4441 gatcctgtct aagccagctt ccccttccct cagttcagct gagtgggaac tggtggctcc
     4501 ctgtcatagc ctagcagggg cagctatgga accagactgg ggataggcac tcttgtggga
     4561 actgtgcccg acccctcccc cttgctcctc ctcccccacc tcagccttac accgtgcaga
     4621 caatcatttt gtatgtgtta agagccacga tggaaggttt tatacagtaa tttgttacct
     4681 aggaacccac tgtgccctga tttttgtcgt gccaataaag agcccctgct ttata
//
