LOCUS       XM_017597894            3016 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus DNA topoisomerase III beta (Top3b),
            transcript variant X2, mRNA.
ACCESSION   XM_017597894
VERSION     XM_017597894.2
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051346.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_017597894.3.
            
            On Jan 21, 2021 this sequence version replaced XM_017597894.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..3016
                     /gene="Top3b"
                     /note="DNA topoisomerase III beta; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 26
                     ESTs, 3 long SRA reads, 14 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 152 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:287930"
                     /db_xref="RGD:1309468"
     CDS             324..2912
                     /gene="Top3b"
                     /codon_start=1
                     /product="DNA topoisomerase 3-beta-1 isoform X1"
                     /protein_id="XP_017453383.1"
                     /db_xref="GeneID:287930"
                     /db_xref="RGD:1309468"
                     /translation="MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGT
                     FAGQPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFL
                     QVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVLNNAHNGEKTVFRARFSSITDTDI
                     CNAMTRLTEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQ
                     TPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDREIAQMFLNM
                     TKLEKEAWVEATSKKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQVAERLYTQGYI
                     SYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITP
                     MKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPEHFTCMGKTVISP
                     GFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGI
                     GTDASIPVHINNICQRNYVTVENGRRLKPTNLGIVLVHGYHKIDAELVLPTIRSAVEK
                     QLNLIAQGKANYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRC
                     GKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRG
                     KSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTS
                     GPKWKVACNTCSVVAHCFDNAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHT
                     GCIFCDTVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPPGKPNPRRPKDKMSA
                     LAAYFV"
ORIGIN      
        1 tgcgttcgtg agtggccacc gggaaagcac ggccggagcg agaggtgcag cggcacggcg
       61 ggtgagcagt gggttgctgg ctgctcgagg aagcggaaga cgctggcttt cttcgggcat
      121 ttccggaatg gtttcaagtg atataccctg cccagaaagc tacagcagag gccaacagtg
      181 gtccagctat gaaatttgca tgtcctcttg caaggagtca gcatcggcag tcctgttgaa
      241 ctacagagaa gacatttcta agcttttgta ttgctgctct tctgggtgcc acagtctgtg
      301 ggccctgaca gcctggagca ggaatgaaga cggtgctcat ggtagcagaa aagccatccc
      361 tggcacaatc cattgccaaa atcctctccc gaggaaacat gtcctcacac aaagggctaa
      421 atggggcgtg ttcagtgcac aaatacacag gaacctttgc tggccagccc gtccacttca
      481 agatgacatc tgtctgtggt catgtgatga cactggattt tctgggaaag tacaacaagt
      541 gggataaggt agatcctgca gagttattca gccaagctcc aacagagaaa aaggaagcaa
      601 accccaagct gaacatggtc aagttcctgc aggtggaggg cagaggctgt gactacgtcg
      661 tgctgtggct ggactgtgac aaggaaggcg agaatatttg ctttgaggtc cttgatgccg
      721 ttctgcctgt cctgaataat gcccacaatg gtgagaagac agtgttccgg gccaggttca
      781 gttccatcac agacacggac atctgcaatg ccatgacccg cctgactgag ccagaccaca
      841 atgaggcact ctcggtggat gcccgccagg agctagacct gcgcatcggc tgtgcgttca
      901 ctaggtttca aacaaagtat ttccagggaa aatatggcga cttagacagc tctctcatct
      961 cctttgggcc ttgtcagact ccaaccctgg gattctgtgt agaaagacat gataaaatcc
     1021 agtccttcaa accagagacc tactgggtac ttcaggccaa ggttcatact gataaagagg
     1081 agtcactcct tttggactgg gaccgagtta gagttttcga ccgggagatt gcacagatgt
     1141 ttttaaacat gacaaagcta gaaaaagaag cttgggtgga ggctacaagc aagaaagaaa
     1201 aagccaagca gagaccactt gctctgaaca ctgtggagat gttgcgtgtg gccagttcag
     1261 ctctgggtat gggaccacag catgccatgc aagtagctga gagattatac acacagggct
     1321 acatcagcta cccacggaca gaaaccacac actatcccga gaactttgac ctgaagggct
     1381 ctttacgaca acaggccaat cacccatact gggcagactc ggtaaagcag ttgttagcag
     1441 aaggcatcaa ccgccctcgg aaaggtcacg atgctggtga ccatcctccg atcacaccaa
     1501 tgaagtcagc tacagaggct gaattaggag gtgacgcttg gcgactctac gagtacatca
     1561 ccagacactt catcgccaca gtgagccatg actgcaagta tctccagagc accatctcct
     1621 tcaggatcgg gcccgagcac ttcacatgca tgggaaagac agttatttcc ccaggcttca
     1681 cagagatcat gccttggcag agcgtgcccc tggaagagag tctgcccaca tgccagaagg
     1741 gcgatacctt cactgtgggt gaggtgaaga tgctagagaa gcagacgagc cccccggact
     1801 atctgactga ggctgagctc atcaccctca tggagaagca tggcatcggg acggatgcca
     1861 gtatccctgt gcacatcaac aacatctgcc agcgcaacta cgtcacagtg gagaacggcc
     1921 gccggctcaa gcccaccaac ctcggcatag ttctggtgca cggctaccac aagattgatg
     1981 cagagctggt gctccccacc atccgcagcg cagtggagaa gcagctgaac cttattgccc
     2041 agggcaaagc caactaccac caggtgctgg gccacaccct ggatatcttc aagagaaagt
     2101 tccactactt tgttgattct attgctggca tggacgagtt aatggaggta tccttttcac
     2161 ccctggctgc cactggcaag cccctctcgc gctgtgggaa gtgccatcga tttatgaagt
     2221 atatccaggc caagccaagc cgcctgcact gctcccactg cgatgagacc tacaccctcc
     2281 cccagaatgg taccatcaag ctctacaagg agctgcggtg cccattggat gactttgaac
     2341 tggtcttgtg gtcctcaggc tcccggggca agagctaccc cctctgtccc tactgctata
     2401 accacccacc cttccgagac atgaagaaag gcatgggctg caatgagtgc acacacccta
     2461 cgtgccagca ctcactgagt atgctgggca ttggccagtg cgtggagtgc gagaatggag
     2521 tcctggttct ggaccccacc tccggtccca agtggaaggt ggcctgcaat acatgcagcg
     2581 tagtggcaca ctgctttgac aatgcccacc gagtacgggt gtctgctgac acctgcaaca
     2641 cctgcgaggc tgccttgctc gatgtagact tcaacaaggc caagtctccc cttcctggca
     2701 acgagacaca gcacacaggc tgcatcttct gtgacaccgt cttccaggag ctggtggagc
     2761 tgaagcatgc agcctcctgc caccccatgc accgaggtgg tcctggaagg aggcagggtc
     2821 gtgggcgggc ccggggccgg agacctccag gaaagcccaa ccccaggcgg ccaaaagaca
     2881 agatgtcagc cctggctgct tactttgtat aactacccac cttgccccac tctagccccc
     2941 gttgatgtag acatgtcgtg gtgatctttg agccactgaa accattaaaa tgctttttat
     3001 tttgtattct ctggaa
//
