LOCUS       XM_039087264            4174 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus DNA topoisomerase III alpha (Top3a),
            transcript variant X2, mRNA.
ACCESSION   XM_039087264
VERSION     XM_039087264.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051345.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039087264.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4174
                     /gene="Top3a"
                     /note="DNA topoisomerase III alpha; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 7
                     ESTs, 1 Protein, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 5 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:303194"
                     /db_xref="RGD:1309320"
     CDS             16..2862
                     /gene="Top3a"
                     /codon_start=1
                     /product="DNA topoisomerase 3-alpha isoform X2"
                     /protein_id="XP_038943192.1"
                     /db_xref="GeneID:303194"
                     /db_xref="RGD:1309320"
                     /translation="MPLINKEGLSKFNKIYEFDYHLYGQNVTMIMTSVSGHLLAHDFQ
                     MQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETQHCQALVIWTDCDREGENIG
                     FEIIHVCKAVKPNLQVLRARFSEITHHAVRAACEHLTEPDQRVSDAVDVRQELDLRIG
                     AAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHRIKVT
                     HDHKDGTVEFNWKRYRLFNHTACLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVE
                     LEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPKDLNLVTLVEQQTQDPHW
                     GAFAQNILERGGPTPRNGNKSDQAHPPIHPTKYTSSLQGDDRRLYEFIVRHFLACCSQ
                     DAQGQETTVEIDIAQERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGFRFQPSTV
                     EMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFLPGH
                     LGMGLVEGYDSMGYEMSKPNLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAV
                     AKAKKLDEALSQYLGERTEVAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSC
                     TGFPECRSAVWFPDSVLEASRDSSVCSVCQPAPVYRLKLKFKRGSLPPSMPLEFVGCI
                     GGCDETLKEIFGLRFSRAPPRASQPSGHLQASQALNRMDSSQRSLSQPLVNRHTGPSK
                     AVAQTLLPPTAAAEGNSVTCNCGREAVLLTVRKQGPNQGRHFYKCSGGDCNFFLWADS
                     SHPTGGGPPTSVSRPPGNSVGCPSGFGSHMDGFGSLGGDGDGGASCLCGQPAVTRTVQ
                     KDGPNKGRQFHTCAKPREQQCGFFQWVDENMAPGSFAAPAWPGGRGKAQRPEAASKRQ
                     RAGSLDAGSTVKKPRKCSLCHQPGHTRPFCPQNR"
ORIGIN      
        1 ctctctctaa ccctcatgcc tcttataaat aaagaaggcc tttctaaatt caacaagatt
       61 tatgaatttg actatcatct gtatggccag aatgttacta tgataatgac ttcagtctct
      121 ggacatttgc tggctcatga cttccagatg cagtttcgga aatggcagag ctgcaatccc
      181 cttgtcctct ttgaagcaga aattgaaaag tattgcccag aaaattttat agacatcaag
      241 aaaactctgg aacgagagac acagcattgt caggcattgg tgatctggac tgactgtgac
      301 agagaaggtg aaaacatcgg gtttgagatt atccatgtat gcaaggctgt aaagcccaat
      361 ctacaggtgc tgagggcccg cttctctgag atcacacacc atgctgtcag ggcagcctgt
      421 gagcatctga ctgagcctga ccagagagta agtgacgcag tggatgtgag gcaagagctg
      481 gatctgagga tcggggctgc cttcacaagg ttccagaccc tgcggctcca gaggattttc
      541 cctgaggtgc tggcggagca gctcatcagc tatggcagct gccagttccc aacactgggg
      601 tttgtggtgg aaaggttcaa ggccattcag gcttttgtgc cggaagtctt ccacagaatt
      661 aaagtaactc atgaccacaa agatgggact gtagagttca actggaaaag ataccgtctc
      721 tttaaccaca cagcttgtct tgtcctttat cagttgtgta tggaggatcc catggcaacc
      781 gtggtagagg tcaggtccaa gccaaagagc aagtggcggc ctcaagcgtt ggacactgtg
      841 gagctagaga aactggcgtc tcgaaaattg agaataaatg ccaaagaaac catgaggatt
      901 gcggaaaagc tctatacaca agggtacatc agctatccca ggacagaaac aaacatcttc
      961 cccaaagact taaacctggt tacattggtg gaacagcaga cccaggatcc acactggggg
     1021 gcttttgctc agaacattct agagcgaggc ggccccactc cacgaaatgg gaacaaatct
     1081 gatcaagctc accctcccat tcaccccacc aaatacacca gcagcctgca gggagatgat
     1141 cggcgactgt atgagttcat cgttcgccat ttcctggctt gctgctctca ggatgctcag
     1201 gggcaagaga ctaccgtgga gatcgacatt gcccaggagc gctttgtagc ccatggcctc
     1261 ataatcctgg cccgtaacta tctagatgtg tatccgtatg accactggag tgacaagctc
     1321 ctccctgtct atgagcaagg cttccgcttt cagcccagca ctgtggaaat ggtggatggg
     1381 gagactagcc caccccaact gcttaccgaa gctgacctca tcgccctcat ggagaaacat
     1441 ggtattggta ctgatgcaac tcatgcagaa cacatcgaga ccatcaaagc ccggatgtat
     1501 gtgggactca cctcagacaa gcggttcctc cctgggcacc tgggcatggg acttgtggaa
     1561 ggttatgatt ccatgggtta cgagatgtcc aagcctaacc tccgtgccga gctggaagct
     1621 gatctcaagc tgatctgtga gggcaagaag gataagtttc aggttctaag gcagcaagtg
     1681 cagaaataca agcaggtgtt cattgaagca gtggcgaagg caaagaagct ggatgaggcc
     1741 ttatctcagt acttaggaga aaggacagag gtggctcagc aagaagagat ctacccagcc
     1801 atgccagagc ctgttcggaa gtgcccacag tgtaacaagg atatggtcct caagaccaag
     1861 aagagtggtg ggttctacct tagctgcacg gggttcccag aatgtcgctc agccgtgtgg
     1921 tttcctgact cggtgctgga ggccagcagg gacagcagtg tatgttccgt ttgtcagcca
     1981 gcccctgtgt acaggttgaa gttgaagttt aagcgtggta gccttccccc atctatgccc
     2041 ctggagtttg tcggctgcat aggtggatgt gatgagacct tgaaggaaat cttcggcctg
     2101 cgattttcac gggctccacc aagagcgagc cagccctctg gccacctgca ggccagccag
     2161 gccctcaaca ggatggacag cagtcagcgt agcctgtctc aacctctggt taacagacat
     2221 actggacctt cgaaggcagt ggcccaaacc ttgctaccac ccactgctgc tgctgagggc
     2281 aactctgtga cctgcaactg tggccgggag gctgtgctgc tcaccgtccg caagcagggc
     2341 cccaaccagg gccggcactt ctataagtgc agtggtggtg actgcaactt cttcctgtgg
     2401 gctgacagca gccaccccac tggaggaggg ccccccacct ctgtatcaag acccccaggc
     2461 aactcggtag gatgcccctc aggcttcggc agccacatgg atgggttcgg cagccttggt
     2521 ggtgacggtg atggaggtgc atcctgcctg tgcgggcagc ctgctgtcac acggactgta
     2581 cagaaggatg ggcccaacaa aggacgccag ttccacacct gtgccaagcc acgggagcag
     2641 cagtgtggct tctttcagtg ggtggatgag aacatggccc cagggagttt tgcagcccca
     2701 gcatggccag gaggcagagg aaaagcccag agaccagagg ctgcaagcaa aagacaaaga
     2761 gctggttcct tagatgcagg gtctacagta aaaaagcctc ggaaatgtag tctttgccac
     2821 cagcctggac acacccggcc cttttgtcct cagaacagat gatggcaggt gaggtggagg
     2881 aagtcaccta accagaaatg aactaactgg accaggtgac catccactgt cctggaaacc
     2941 tagggaggta ttgggtttta gagttggcct tcctttgttg aggagtacaa agaccagaca
     3001 cgtctggaaa atctggctgg ctctgctgga tgccgttgct ctgtctggct caggagtgac
     3061 tggctgcgca ctaatggaaa gtgagaagat gagcagtcag catgtaagca gagctgacct
     3121 cagtgacctt ccaaacccgg gccgtggcca ctgtctgcca catgagaagc agcaccggct
     3181 ctgggcatcc cagtgggcct tttctgacag ctgtttgaga agtggcagaa gcctcccctc
     3241 ctgctcagcc taggacaccc atagctcatg tgccttaatt taaaaaaaaa aggggggggc
     3301 tggggattta gctcagtggt agagtacttg cctaggaagc acaaggccct gggttcggtc
     3361 cccagctccg aaaaaaaaaa aaaaaatggg ggttgggaat ttagctcagt ggtagagcgc
     3421 ttgcctagta agcgcaaggc cctgggtttg gtcctcagct ccggggggaa aaaaaaaatc
     3481 catccacatt ttctggtggc attcaggctg gggatgccag caggagccag tgaaggccaa
     3541 agggcctctt cttgggactc tccccatcca tctctgcact ctcttgcagg gttcttccct
     3601 gctgttgtcc cttgaagccg gcagattcca ggatactgac tggcctttgc agaagaggct
     3661 gtgtcctttg tttgcgctca caaacttcta gccagccatg ttagctttgt ccacacttga
     3721 gaagtgaagt gtgaattgtt ccttgattta caagttcatg aagcaactgg ggttgttctc
     3781 aggtcagagc aatagtcttg aatagatagg aactctcagc ctggctgcca gcaggttctc
     3841 cagtgaatat tcctaccatt gctcattggg taccatctgc agcctgcatt tagggtctga
     3901 ccctggtcca tcactcacag ctggcctacc tgtttgatca cttcagtaga cacctcacgg
     3961 acttgctgtg cctagcacct ttcccttcgc acaatgttca caggatttgt tgagagtgag
     4021 tcattcattt tgttgttgtt gttttgtttt gaaacggttt ttctgtgtag ccctggctat
     4081 cctggaatgt gctttataga ccaggctgac ctcaagctca gagaccatgt gcctctgcct
     4141 cctgagggct gggaataaag gtgtaccatg ccca
//
