LOCUS       XM_039087263            4507 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus DNA topoisomerase III alpha (Top3a),
            transcript variant X1, mRNA.
ACCESSION   XM_039087263
VERSION     XM_039087263.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051345.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Jan 25, 2024 this sequence was replaced by
            NM_001427456.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4507
                     /gene="Top3a"
                     /note="DNA topoisomerase III alpha; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 7
                     ESTs, 4 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 172
                     samples with support for all annotated introns"
                     /db_xref="GeneID:303194"
                     /db_xref="RGD:1309320"
     CDS             184..3195
                     /gene="Top3a"
                     /codon_start=1
                     /product="DNA topoisomerase 3-alpha isoform X1"
                     /protein_id="XP_038943191.1"
                     /db_xref="GeneID:303194"
                     /db_xref="RGD:1309320"
                     /translation="MIFPVTLLAFQWQRRPGGRALSRAAMEVAFRGVRKVLCVAEKND
                     AAKGIADLLSNGRMRRKEGLSKFNKIYEFDYHLYGQNVTMIMTSVSGHLLAHDFQMQF
                     RKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETQHCQALVIWTDCDREGENIGFEI
                     IHVCKAVKPNLQVLRARFSEITHHAVRAACEHLTEPDQRVSDAVDVRQELDLRIGAAF
                     TRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHRIKVTHDH
                     KDGTVEFNWKRYRLFNHTACLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVELEK
                     LASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPKDLNLVTLVEQQTQDPHWGAF
                     AQNILERGGPTPRNGNKSDQAHPPIHPTKYTSSLQGDDRRLYEFIVRHFLACCSQDAQ
                     GQETTVEIDIAQERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGFRFQPSTVEMV
                     DGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFLPGHLGM
                     GLVEGYDSMGYEMSKPNLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKA
                     KKLDEALSQYLGERTEVAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCTGF
                     PECRSAVWFPDSVLEASRDSSVCSVCQPAPVYRLKLKFKRGSLPPSMPLEFVGCIGGC
                     DETLKEIFGLRFSRAPPRASQPSGHLQASQALNRMDSSQRSLSQPLVNRHTGPSKAVA
                     QTLLPPTAAAEGNSVTCNCGREAVLLTVRKQGPNQGRHFYKCSGGDCNFFLWADSSHP
                     TGGGPPTSVSRPPGNSVGCPSGFGSHMDGFGSLGGDGDGGASCLCGQPAVTRTVQKDG
                     PNKGRQFHTCAKPREQQCGFFQWVDENMAPGSFAAPAWPGGRGKAQRPEAASKRQRAG
                     SLDAGSTVKKPRKCSLCHQPGHTRPFCPQNR"
ORIGIN      
        1 ggaagttgtc tgtgagcgcg agtttaagcg ggactcggag gtgtgggttt ctgatccagg
       61 aggcagtttc ctggaaaaaa atgcattatt aaagtggctc tcagagggct gcctcgtggc
      121 tttaactctg ggtgctccaa catgcatggg cgcccgtgga gttgctggcg cactgtctag
      181 ggaatgatct tcccagtcac cctcttagcg tttcagtggc agcgacggcc tggaggccgt
      241 gccctgtccc gcgctgccat ggaagtagcc ttccgaggag tgcgaaaagt cctctgcgtg
      301 gcggagaaaa acgacgctgc caaaggaatc gccgacttgc tatccaacgg tcgtatgagg
      361 cggaaagaag gcctttctaa attcaacaag atttatgaat ttgactatca tctgtatggc
      421 cagaatgtta ctatgataat gacttcagtc tctggacatt tgctggctca tgacttccag
      481 atgcagtttc ggaaatggca gagctgcaat ccccttgtcc tctttgaagc agaaattgaa
      541 aagtattgcc cagaaaattt tatagacatc aagaaaactc tggaacgaga gacacagcat
      601 tgtcaggcat tggtgatctg gactgactgt gacagagaag gtgaaaacat cgggtttgag
      661 attatccatg tatgcaaggc tgtaaagccc aatctacagg tgctgagggc ccgcttctct
      721 gagatcacac accatgctgt cagggcagcc tgtgagcatc tgactgagcc tgaccagaga
      781 gtaagtgacg cagtggatgt gaggcaagag ctggatctga ggatcggggc tgccttcaca
      841 aggttccaga ccctgcggct ccagaggatt ttccctgagg tgctggcgga gcagctcatc
      901 agctatggca gctgccagtt cccaacactg gggtttgtgg tggaaaggtt caaggccatt
      961 caggcttttg tgccggaagt cttccacaga attaaagtaa ctcatgacca caaagatggg
     1021 actgtagagt tcaactggaa aagataccgt ctctttaacc acacagcttg tcttgtcctt
     1081 tatcagttgt gtatggagga tcccatggca accgtggtag aggtcaggtc caagccaaag
     1141 agcaagtggc ggcctcaagc gttggacact gtggagctag agaaactggc gtctcgaaaa
     1201 ttgagaataa atgccaaaga aaccatgagg attgcggaaa agctctatac acaagggtac
     1261 atcagctatc ccaggacaga aacaaacatc ttccccaaag acttaaacct ggttacattg
     1321 gtggaacagc agacccagga tccacactgg ggggcttttg ctcagaacat tctagagcga
     1381 ggcggcccca ctccacgaaa tgggaacaaa tctgatcaag ctcaccctcc cattcacccc
     1441 accaaataca ccagcagcct gcagggagat gatcggcgac tgtatgagtt catcgttcgc
     1501 catttcctgg cttgctgctc tcaggatgct caggggcaag agactaccgt ggagatcgac
     1561 attgcccagg agcgctttgt agcccatggc ctcataatcc tggcccgtaa ctatctagat
     1621 gtgtatccgt atgaccactg gagtgacaag ctcctccctg tctatgagca aggcttccgc
     1681 tttcagccca gcactgtgga aatggtggat ggggagacta gcccacccca actgcttacc
     1741 gaagctgacc tcatcgccct catggagaaa catggtattg gtactgatgc aactcatgca
     1801 gaacacatcg agaccatcaa agcccggatg tatgtgggac tcacctcaga caagcggttc
     1861 ctccctgggc acctgggcat gggacttgtg gaaggttatg attccatggg ttacgagatg
     1921 tccaagccta acctccgtgc cgagctggaa gctgatctca agctgatctg tgagggcaag
     1981 aaggataagt ttcaggttct aaggcagcaa gtgcagaaat acaagcaggt gttcattgaa
     2041 gcagtggcga aggcaaagaa gctggatgag gccttatctc agtacttagg agaaaggaca
     2101 gaggtggctc agcaagaaga gatctaccca gccatgccag agcctgttcg gaagtgccca
     2161 cagtgtaaca aggatatggt cctcaagacc aagaagagtg gtgggttcta ccttagctgc
     2221 acggggttcc cagaatgtcg ctcagccgtg tggtttcctg actcggtgct ggaggccagc
     2281 agggacagca gtgtatgttc cgtttgtcag ccagcccctg tgtacaggtt gaagttgaag
     2341 tttaagcgtg gtagccttcc cccatctatg cccctggagt ttgtcggctg cataggtgga
     2401 tgtgatgaga ccttgaagga aatcttcggc ctgcgatttt cacgggctcc accaagagcg
     2461 agccagccct ctggccacct gcaggccagc caggccctca acaggatgga cagcagtcag
     2521 cgtagcctgt ctcaacctct ggttaacaga catactggac cttcgaaggc agtggcccaa
     2581 accttgctac cacccactgc tgctgctgag ggcaactctg tgacctgcaa ctgtggccgg
     2641 gaggctgtgc tgctcaccgt ccgcaagcag ggccccaacc agggccggca cttctataag
     2701 tgcagtggtg gtgactgcaa cttcttcctg tgggctgaca gcagccaccc cactggagga
     2761 gggcccccca cctctgtatc aagaccccca ggcaactcgg taggatgccc ctcaggcttc
     2821 ggcagccaca tggatgggtt cggcagcctt ggtggtgacg gtgatggagg tgcatcctgc
     2881 ctgtgcgggc agcctgctgt cacacggact gtacagaagg atgggcccaa caaaggacgc
     2941 cagttccaca cctgtgccaa gccacgggag cagcagtgtg gcttctttca gtgggtggat
     3001 gagaacatgg ccccagggag ttttgcagcc ccagcatggc caggaggcag aggaaaagcc
     3061 cagagaccag aggctgcaag caaaagacaa agagctggtt ccttagatgc agggtctaca
     3121 gtaaaaaagc ctcggaaatg tagtctttgc caccagcctg gacacacccg gcccttttgt
     3181 cctcagaaca gatgatggca ggtgaggtgg aggaagtcac ctaaccagaa atgaactaac
     3241 tggaccaggt gaccatccac tgtcctggaa acctagggag gtattgggtt ttagagttgg
     3301 ccttcctttg ttgaggagta caaagaccag acacgtctgg aaaatctggc tggctctgct
     3361 ggatgccgtt gctctgtctg gctcaggagt gactggctgc gcactaatgg aaagtgagaa
     3421 gatgagcagt cagcatgtaa gcagagctga cctcagtgac cttccaaacc cgggccgtgg
     3481 ccactgtctg ccacatgaga agcagcaccg gctctgggca tcccagtggg ccttttctga
     3541 cagctgtttg agaagtggca gaagcctccc ctcctgctca gcctaggaca cccatagctc
     3601 atgtgcctta atttaaaaaa aaaagggggg ggctggggat ttagctcagt ggtagagtac
     3661 ttgcctagga agcacaaggc cctgggttcg gtccccagct ccgaaaaaaa aaaaaaaaat
     3721 gggggttggg aatttagctc agtggtagag cgcttgccta gtaagcgcaa ggccctgggt
     3781 ttggtcctca gctccggggg gaaaaaaaaa atccatccac attttctggt ggcattcagg
     3841 ctggggatgc cagcaggagc cagtgaaggc caaagggcct cttcttggga ctctccccat
     3901 ccatctctgc actctcttgc agggttcttc cctgctgttg tcccttgaag ccggcagatt
     3961 ccaggatact gactggcctt tgcagaagag gctgtgtcct ttgtttgcgc tcacaaactt
     4021 ctagccagcc atgttagctt tgtccacact tgagaagtga agtgtgaatt gttccttgat
     4081 ttacaagttc atgaagcaac tggggttgtt ctcaggtcag agcaatagtc ttgaatagat
     4141 aggaactctc agcctggctg ccagcaggtt ctccagtgaa tattcctacc attgctcatt
     4201 gggtaccatc tgcagcctgc atttagggtc tgaccctggt ccatcactca cagctggcct
     4261 acctgtttga tcacttcagt agacacctca cggacttgct gtgcctagca cctttccctt
     4321 cgcacaatgt tcacaggatt tgttgagagt gagtcattca ttttgttgtt gttgttttgt
     4381 tttgaaacgg tttttctgtg tagccctggc tatcctggaa tgtgctttat agaccaggct
     4441 gacctcaagc tcagagacca tgtgcctctg cctcctgagg gctgggaata aaggtgtacc
     4501 atgccca
//
