LOCUS       NM_001393768            5557 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus DNA topoisomerase II alpha (Top2a), mRNA.
ACCESSION   NM_001393768
VERSION     NM_001393768.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Kawano,S., Kato,Y., Okada,N., Sano,K., Tsutsui,K., Tsutsui,K.M. and
            Ikeda,S.
  TITLE     DNA-binding activity of rat DNA topoisomerase II alpha C-terminal
            domain contributes to efficient DNA catenation in vitro
  JOURNAL   J Biochem 159 (3), 363-369 (2016)
   PUBMED   26527691
  REMARK    GeneRIF: C-terminal domain of rat topo IIalpha is associated with
            efficient DNA catenation in vitro, based on comparison of wild-type
            rat topo IIalpha and its deletion mutants.
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Mladenovic,M., Stankovic,N., Matic,S., Stanic,S., Mihailovic,M.,
            Mihailovic,V., Katanic,J., Boroja,T. and Vukovic,N.
  TITLE     Newly discovered chroman-2,4-diones neutralize the in vivo DNA
            damage induced by alkylation through the inhibition of
            Topoisomerase IIalpha: A story behind the molecular modeling
            approach
  JOURNAL   Biochem Pharmacol 98 (1), 243-266 (2015)
   PUBMED   26319574
  REMARK    GeneRIF: The genotoxic and antigenotoxic effects of 8
            chroman-2,4-diones are reported; the role of their TOPIIalpha
            inhibitory effect is described.
REFERENCE   3  (bases 1 to 5557)
  AUTHORS   Houlard,M., Godwin,J., Metson,J., Lee,J., Hirano,T. and Nasmyth,K.
  TITLE     Condensin confers the longitudinal rigidity of chromosomes
  JOURNAL   Nat Cell Biol 17 (6), 771-781 (2015)
   PUBMED   25961503
REFERENCE   4  (bases 1 to 5557)
  AUTHORS   Rochette,L., Guenancia,C., Gudjoncik,A., Hachet,O., Zeller,M.,
            Cottin,Y. and Vergely,C.
  TITLE     Anthracyclines/trastuzumab: new aspects of cardiotoxicity and
            molecular mechanisms
  JOURNAL   Trends Pharmacol Sci 36 (6), 326-348 (2015)
   PUBMED   25895646
  REMARK    Review article
REFERENCE   5  (bases 1 to 5557)
  AUTHORS   Ogawa,Y., Kawano,Y., Yamazaki,Y. and Onishi,Y.
  TITLE     Shikonin shortens the circadian period: possible involvement of
            Top2 inhibition
  JOURNAL   Biochem Biophys Res Commun 443 (1), 339-343 (2014)
   PUBMED   24321095
REFERENCE   6  (bases 1 to 5557)
  AUTHORS   Baloch,Z., Cohen,S., Fresa,K. and Coffman,F.D.
  TITLE     Modulation of topoisomerase activities by tumor necrosis factor
  JOURNAL   Cell Immunol 160 (1), 98-103 (1995)
   PUBMED   7842491
REFERENCE   7  (bases 1 to 5557)
  AUTHORS   Adachi,N., Ikeda,H. and Kikuchi,A.
  TITLE     Mutant isolation of mouse DNA topoisomerase II alpha in yeast
  JOURNAL   Nucleic Acids Res 22 (20), 4229-4233 (1994)
   PUBMED   7937150
REFERENCE   8  (bases 1 to 5557)
  AUTHORS   Watanabe,M., Tsutsui,K., Tsutsui,K. and Inoue,Y.
  TITLE     Differential expressions of the topoisomerase II alpha and II beta
            mRNAs in developing rat brain
  JOURNAL   Neurosci Res 19 (1), 51-57 (1994)
   PUBMED   8008235
REFERENCE   9  (bases 1 to 5557)
  AUTHORS   Adachi,N., Miyaike,M., Ikeda,H. and Kikuchi,A.
  TITLE     Characterization of cDNA encoding the mouse DNA topoisomerase II
            that can complement the budding yeast top2 mutation
  JOURNAL   Nucleic Acids Res 20 (20), 5297-5303 (1992)
   PUBMED   1331984
REFERENCE   10 (bases 1 to 5557)
  AUTHORS   Ura,K. and Hirose,S.
  TITLE     Possible role of DNA topoisomerase II on transcription of the
            homeobox gene Hox-2.1 in F9 embryonal carcinoma cells
  JOURNAL   Nucleic Acids Res 19 (22), 6087-6092 (1991)
   PUBMED   1683482
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643294.41650.1,
                                           SRR26643429.38884.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               JAXUCZ010000010.1  84472964-84473093   c
            131-283             JAXUCZ010000010.1  84471761-84471913   c
            284-374             JAXUCZ010000010.1  84471121-84471211   c
            375-438             JAXUCZ010000010.1  84470327-84470390   c
            439-584             JAXUCZ010000010.1  84468466-84468611   c
            585-682             JAXUCZ010000010.1  84466649-84466746   c
            683-895             JAXUCZ010000010.1  84466246-84466458   c
            896-1069            JAXUCZ010000010.1  84464937-84465110   c
            1070-1171           JAXUCZ010000010.1  84464707-84464808   c
            1172-1309           JAXUCZ010000010.1  84464437-84464574   c
            1310-1448           JAXUCZ010000010.1  84462592-84462730   c
            1449-1606           JAXUCZ010000010.1  84460546-84460703   c
            1607-1732           JAXUCZ010000010.1  84460110-84460235   c
            1733-1843           JAXUCZ010000010.1  84459699-84459809   c
            1844-1949           JAXUCZ010000010.1  84459478-84459583   c
            1950-2059           JAXUCZ010000010.1  84459283-84459392   c
            2060-2152           JAXUCZ010000010.1  84458518-84458610   c
            2153-2267           JAXUCZ010000010.1  84456784-84456898   c
            2268-2389           JAXUCZ010000010.1  84456562-84456683   c
            2390-2538           JAXUCZ010000010.1  84455964-84456112   c
            2539-2770           JAXUCZ010000010.1  84453353-84453584   c
            2771-2905           JAXUCZ010000010.1  84453063-84453197   c
            2906-3106           JAXUCZ010000010.1  84452745-84452945   c
            3107-3302           JAXUCZ010000010.1  84452046-84452241   c
            3303-3394           JAXUCZ010000010.1  84450561-84450652   c
            3395-3556           JAXUCZ010000010.1  84449975-84450136   c
            3557-3640           JAXUCZ010000010.1  84449766-84449849   c
            3641-3823           JAXUCZ010000010.1  84447804-84447986   c
            3824-3914           JAXUCZ010000010.1  84446955-84447045   c
            3915-4067           JAXUCZ010000010.1  84445765-84445917   c
            4068-4191           JAXUCZ010000010.1  84444705-84444828   c
            4192-4235           JAXUCZ010000010.1  84444538-84444581   c
            4236-4370           JAXUCZ010000010.1  84444042-84444176   c
            4371-4570           JAXUCZ010000010.1  84443197-84443396   c
            4571-5557           JAXUCZ010000010.1  84441954-84442940   c
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q31"
     gene            1..5557
                     /gene="Top2a"
                     /note="DNA topoisomerase II alpha"
                     /db_xref="GeneID:360243"
                     /db_xref="RGD:62048"
     exon            1..130
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..4696
                     /gene="Top2a"
                     /EC_number="5.6.2.2"
                     /note="DNA topoisomerase II, alpha isozyme; topoisomerase
                     (DNA) II alpha; topoisomerase (DNA) 2 alpha"
                     /codon_start=1
                     /product="DNA topoisomerase 2-alpha"
                     /protein_id="NP_001380697.1"
                     /db_xref="GeneID:360243"
                     /db_xref="RGD:62048"
                     /translation="MELSPLQPVNENMLLNKKKNEDGKKRLSVERIYQKKTQLEHILL
                     RPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMS
                     CIRVTIDAENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGG
                     RNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITF
                     QPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKD
                     KVDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVS
                     KLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQ
                     LSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRN
                     SAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINN
                     IIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRH
                     RFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSANPNHKKWKVKYYKGLGTSTSKEAK
                     EYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPED
                     YLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKRE
                     VKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKD
                     SASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIG
                     TGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGEVAI
                     LNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMT
                     EEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYG
                     LRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKA
                     WKEAQQKVPEEEENEENEESESESSSPAAESGPTFNYLLDMPLWYLTKEKKDELCKQR
                     DEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGLPGKGVKAKGKKAQ
                     ISEVLPSPVGKRVIPQVTMEMRAEAEKKIRRKIKSENVEGTPAEDGAEPGGLRQRLEK
                     RQKREPGTRAKKQTTLPFKPIKKGKKKNPWSDSESDMSSNESNFDVPPREKEPRIAAT
                     KAKFTADLDSDDDFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDL
                     ESDGKDSVPASPGASAADVPAETEPSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVP
                     KETKSDSALNAHVSKKPAPAKAKNSRKRKPSSSDSSDSEFEKAISKGATSKKPKGEER
                     DFHVDLDDTVAPRAKSGRARKPIKYLEESDDDLF"
     exon            131..283
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            284..374
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            375..438
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            439..584
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            585..682
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            683..895
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1069
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1171
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1309
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1448
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1606
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1732
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1843
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1949
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2059
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2152
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2267
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2389
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2538
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2539..2770
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2905
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..3106
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3302
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3303..3394
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3556
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3557..3640
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3641..3823
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..3914
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3915..4067
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4068..4191
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4192..4235
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4236..4370
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4570
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4571..5557
                     /gene="Top2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcctaaccg acgcacgtct gtggagaagc gtccaagtcg ccggtcgcct agcgtttctc
       61 actgattttg tcgcttttag gattctcgag ccccttcagg acggtcacca tggagttgtc
      121 accgctgcag cctgtaaatg aaaatatgct actcaacaaa aagaagaatg aagatggcaa
      181 gaaaagactg tccgttgaaa gaatctatca gaaaaaaacc caactggaac atatactgct
      241 ccgcccagat acctacatcg gctctgtgga gttagtgacc cagcaaatgt gggtttacga
      301 tgaagatgtt ggtattaact acagggaagt cacttttgtt cctggtttgt ataaaatctt
      361 tgatgaaatt ctagttaatg ctgctgataa caaacaaagg gacccaaaaa tgtcttgcat
      421 tagagtcaca attgatgcag aaaataattt aattagtata tggaataatg gaaaaggaat
      481 tcctgttgtt gaacacaaag tggagaaaat gtatgtccca gctctcatat ttggacagct
      541 cctgacctcc agtaactacg atgatgatga gaagaaagtg acaggtggtc gaaatggcta
      601 tggagctaaa ctgtgtaaca tattcagcac caaatttact gtggaaacag ccagtaggga
      661 atacaagaaa atgttcaaac agacatggat ggataacatg gggagagctg gtgacatgga
      721 gctcaagccc tttagcggag aggattacac gtgtattacc ttccagcctg acttatctaa
      781 gtttaaaatg caaagcctgg acaaagatat tgtcgcactg atggtcagaa gagcatacga
      841 tattgctggt tccaccaaag atgtaaaagt ctttcttaat ggaaacaggc tgccagtcaa
      901 agggttccgt agttacgtgg atatgtattt gaaggataag gttgatgaaa ctgggaacgc
      961 actgaaggtg gtgcatgaac aagtaaatcc ccggtgggaa gtgtgcttaa caatgagcga
     1021 gaaaggcttt cagcagatta gcttcgtcaa cagcatcgct acttctaagg gtggcagaca
     1081 tgtcgattat gtagccgatc agattgtgag taaactcgtt gatgtggtga aaaagaagaa
     1141 caagggaggt gttgcagtga aggcccatca ggtgaagaat cacatgtgga tttttgtaaa
     1201 tgccttaatt gaaaacccaa cctttgactc tcaaacaaaa gaaaacatga ctttacaagc
     1261 caagagcttt ggatcaactt gtcaattaag tgaaaaattc atcaaagctg caattggttg
     1321 tggtattgtc gaaagcatac taaactgggt gaagtttaag gcccaaatcc aattaaacaa
     1381 gaagtgttca gctgtaaaac ataacaggat caagggaatc cccaaacttg acgacgccaa
     1441 tgacgcaggg agccgaaact ctgctgaatg cacacttatc ctgactgaag gggactcagc
     1501 caaaactctg gcagtctcag gacttggagt ggttggaaga gacaaatatg gggtgttccc
     1561 tctcagagga aaaatactca atgtgcgaga agcttctcat aaacagatca tggaaaatgc
     1621 tgaaattaac aatatcatca agattgtggg gcttcagtac aagaaaaact atgaggatga
     1681 agattcattg aaaactcttc gttatgggaa gatcatgatt atgacagatc aggaccaaga
     1741 tggttcccac atcaaaggct tgctgatcaa ttttatccat cacaattggc catctcttct
     1801 gcgacatcgt tttctagaag aatttatcac tcctattgta aaggtgtcta aaaacaagca
     1861 agaaatagca ttctacagtc ttcccgagtt tgaagaatgg aagagtgcaa atccaaatca
     1921 caaaaaatgg aaagtcaagt actacaaagg tttgggcacg agcacatcaa aggaagctaa
     1981 ggagtatttt gcaaatatga aacgacatcg tattcagttc aaatactctg gccctgaaga
     2041 cgatgctgcg atcagcctgg cctttagtaa aaaacaggtc gatgatcgaa aggaatggtt
     2101 aactaatttc atggaagata gaagacagcg gaagttactt ggccttcctg aggattattt
     2161 gtatggacaa accactatgt acctgaccta taatgatttc attaacaagg aacttatcct
     2221 cttttcaaac tctgataatg aaagatctat cccatctatg gtagatggtt tgaaaccagg
     2281 tcagagaaag gttttgttta cttgtttcaa acggaatgac aagcgagagg tgaaagttgc
     2341 ccagttagct gggtcagtgg cagaaatgtc ctcttatcac catggtgaga tgtcgctgat
     2401 gatgaccatt atcaatttgg ctcagaattt tgtgggtagc aataatttga accttctaca
     2461 gcctattggt cagtttggta ccaggctgca tggtggcaag gactcagcca gccctaggta
     2521 catctttaca atgctcagcc ctttggctcg gttgttattt ccatcaaaag atgatcacac
     2581 gttacggttt ctgtatgacg acaaccagcg tgttgagcct gaatggtaca tccctattat
     2641 acccatggtg ctgataaatg gtgctgaggg aattggtact gggtggtcct gcaaaatccc
     2701 caactttgat gttcgtgaag tcgtaaataa tatcaggcgg ctgttggatg gagaagagcc
     2761 tctgcccatg ctcccaagtt ataagaatta caaaggtact attgaagaac tggcttcaaa
     2821 tcagtatgtg attaatggag aagtagctat tctgaattcc acaaccattg agatcactga
     2881 gcttcccatc agaacatgga cccagacata taaggaacag gttctagaac ctatgttgaa
     2941 tggcaccgag aagacacccc ctctaataac agactatagg gaataccaca cagataccac
     3001 tgtgaagttt gtcataaaga tgactgaaga aaaattggca gaggcagaga gagtgggact
     3061 acacaaagtc ttcaaactcc agactagtct cacttgcaac tctatggtac tttttgacca
     3121 tgttggttgt ttaaagaaat atgacactgt gttggatatt ctaagagact tctttgagct
     3181 caggcttaaa tattatggat taagaaaaga atggcttcta ggaatgcttg gtgcagaatc
     3241 ttctaaactg aataatcaag ctcgctttat attagagaaa atagatggca aaatagtcat
     3301 tgaaaataaa cctaagaaag aattaattaa agttctgatt cagagaggct atgactccga
     3361 ccctgtgaaa gcctggaaag aagctcagca gaaggtccca gaagaagagg aaaatgaaga
     3421 gaatgaagag agcgagagtg aaagcagcag ccctgcagcg gagtctggac cgaccttcaa
     3481 ctaccttctt gatatgcccc tgtggtatct aaccaaggag aagaaggacg agctgtgcaa
     3541 gcaaagggac gagaaggaac aagagctcaa cacgttaaag aaaaagactc catcggattt
     3601 gtggaaggaa gacctggctg cttttgtgga agaactggag gttgttgaag ccaaggaaaa
     3661 acaggatgaa caagtaggac ttcctggaaa gggggtgaaa gcgaagggga agaaagcgca
     3721 gatatctgaa gtcctgcctt ctcctgtggg gaaaagggtc atcccgcagg tgactatgga
     3781 gatgagagct gaggcagaga agaaaatcag gaggaaaatt aagagcgaga atgttgaggg
     3841 cacccctgct gaggatggtg ctgagccagg aggcctccgg cagagactag agaagaggca
     3901 gaagagagag ccaggtacca gggcaaagaa gcaaactaca ttgccattta agcctatcaa
     3961 aaaagggaaa aagaaaaacc cttggtctga ttctgaatca gatatgagca gtaacgaaag
     4021 taattttgat gtccctccac gagagaaaga gccaagaatt gctgcaacaa aagccaaatt
     4081 cacggcggat ttagactctg atgacgattt ctcaggcttg gatgagaagg atgaggatga
     4141 agatttcttc ccattagatg atactccacc taagaccaaa atgcccccaa aaaatactaa
     4201 aaaagcactg aagccacaga agagctccac atcagttgat cttgaaagtg atgggaaaga
     4261 cagtgtgcca gcttctccag gcgcttctgc tgctgacgtc ccagctgaga ctgaaccctc
     4321 caagccgtcc tccaaacaga cagtgggagt gaagaggaca ataaccaaag gtcagtcttt
     4381 aacctccact gctggcacca aaaagagagc tgtaccaaag gagaccaagt cagattcagc
     4441 cttgaatgct catgtctcca aaaagcctgc tcctgccaaa gccaagaaca gtcgcaagag
     4501 gaagccatct tcttctgaca gctcggactc tgagtttgag aaggcgattt ctaaaggtgc
     4561 cacaagcaag aaacccaagg gagaggagcg ggatttccat gtggacttag atgacactgt
     4621 ggctcctcga gcaaaatccg gacgagcaag gaagccgatt aagtacctgg aggagtcgga
     4681 tgacgacctc ttctaagggg tgaggggatt tcaggaatgg ttttactgag ccacgttagt
     4741 tttaaagttc ttccttaacc actctgaatt taattggagg aagacttttt tttaaataag
     4801 aatatgcaag tgagcagggc ccctgtggcc ttcacctttg ttctcaacat actgtcagta
     4861 gtggccgtct cgtgggcatt gtcgtcttct ctgattgtct gttttatgtc tgttttcttt
     4921 ggtctctgaa acctggcttg aatatttttt ggattccaca taattatatg cttactcagc
     4981 acagtagcat ttatcaattt aaactaaagt ggaaatggtt tcaaaagcat tcctgcctcc
     5041 tttcctatcc cactaaatat gaggtaagac agcccttgtt ttcaatttta taggtagaat
     5101 tcagtcataa agagctggtt agttctagta cagatacagt gcccagcctc tgatgtgatg
     5161 gacttttgtt tggccatgag aaattgctcg taccttttga acatcatcag aggctggagg
     5221 aagtcacctt gaccatgtct tcatgactca gcacaagcag caggatgaag aaagttaggc
     5281 cagttacgtg aagtcttgac tcttgtttgg gggtgccttc cgaactcgag atccctgggg
     5341 ggaggcagga ctgttgacat ctccacactc agccaggaag tgtgatccgt gccaatctcc
     5401 gtaattccga cttgtgaatg ccgagacctt ctgtgctgtt gtatatgtta cttaagtgct
     5461 attatcagtg tcttttgtaa atatttaata tgtctgtcta ttcagcttaa ttccaactat
     5521 tttgtacttt aataaatgtt ctaaacatcg aaagtca
//
