LOCUS       NP_072137                767 aa            linear   ROD 31-MAR-2024
DEFINITION  DNA topoisomerase 1 [Rattus norvegicus].
ACCESSION   NP_072137
VERSION     NP_072137.1
DBSOURCE    REFSEQ: accession NM_022615.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 767)
  AUTHORS   Huelsenbeck,S.C., Schorr,A., Roos,W.P., Huelsenbeck,J.,
            Henninger,C., Kaina,B. and Fritz,G.
  TITLE     Rac1 protein signaling is required for DNA damage response
            stimulated by topoisomerase II poisons
  JOURNAL   J Biol Chem 287 (46), 38590-38599 (2012)
   PUBMED   23012366
  REMARK    GeneRIF: Rac1-regulated mechanisms are required for DNA damage
            induction and subsequent activation of the DDR following treatment
            with topo II but not topo I poisons
REFERENCE   2  (residues 1 to 767)
  AUTHORS   Onishi,Y. and Kawano,Y.
  TITLE     Rhythmic binding of Topoisomerase I impacts on the transcription of
            Bmal1 and circadian period
  JOURNAL   Nucleic Acids Res 40 (19), 9482-9492 (2012)
   PUBMED   22904072
REFERENCE   3  (residues 1 to 767)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (residues 1 to 767)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (residues 1 to 767)
  AUTHORS   Casafont,I., Bengoechea,R., Navascues,J., Pena,E., Berciano,M.T.
            and Lafarga,M.
  TITLE     The giant fibrillar center: a nucleolar structure enriched in
            upstream binding factor (UBF) that appears in transcriptionally
            more active sensory ganglia neurons
  JOURNAL   J Struct Biol 159 (3), 451-461 (2007)
   PUBMED   17587596
REFERENCE   6  (residues 1 to 767)
  AUTHORS   Tournier,M.F., Sobczak,J., de Nechaud,B. and Duguet,M.
  TITLE     Comparison of biochemical properties of DNA-topoisomerase I from
            normal and regenerating liver
  JOURNAL   Eur J Biochem 210 (1), 359-364 (1992)
   PUBMED   1332866
REFERENCE   7  (residues 1 to 767)
  AUTHORS   Ivanov,V.A.
  TITLE     Variations in DNA topoisomerase I activity after gamma irradiation
            of mature neurons
  JOURNAL   Neurosci Lett 94 (1-2), 99-103 (1988)
   PUBMED   2853856
REFERENCE   8  (residues 1 to 767)
  AUTHORS   Rose,K.M., Szopa,J., Han,F.S., Cheng,Y.C., Richter,A. and Scheer,U.
  TITLE     Association of DNA topoisomerase I and RNA polymerase I: a possible
            role for topoisomerase I in ribosomal gene transcription
  JOURNAL   Chromosoma 96 (6), 411-416 (1988)
   PUBMED   2851418
REFERENCE   9  (residues 1 to 767)
  AUTHORS   Crespi,M.D., Radicella,J.P., Baldi,A., Charreau,E.H. and Calvo,J.C.
  TITLE     DNA topoisomerase I from Leydig cells is modulated by luteinizing
            hormone and cyclic adenosine monophosphate
  JOURNAL   Mol Cell Endocrinol 53 (3), 221-226 (1987)
   PUBMED   2822514
REFERENCE   10 (residues 1 to 767)
  AUTHORS   Ivanov,V.A., Melnikov,A.A. and Terpilovska,O.N.
  TITLE     DNA topoisomerase I from rat brain neurons
  JOURNAL   Biochim Biophys Acta 866 (2-3), 154-160 (1986)
   PUBMED   3006775
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.16230.1,
                                           SRR26643285.14146.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     Protein         1..767
                     /product="DNA topoisomerase 1"
                     /EC_number="5.6.2.1"
                     /note="topoisomerase (DNA) I"
                     /calculated_mol_wt=90629
     Region          1..200
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine.
                     /evidence=ECO:0000250|UniProtKB:P11387; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            2
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P11387; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            10
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            59
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P11387; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            114
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            174
                     /site_type="acetylation"
                     /note="N6-acetyllysine, alternate.
                     /evidence=ECO:0000250|UniProtKB:Q04750; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Region          218..432
                     /region_name="Topoisomer_IB_N_htopoI_like"
                     /note="Topoisomer_IB_N_htopoI_like : N-terminal DNA
                     binding fragment found in eukaryotic DNA topoisomerase
                     (topo) IB proteins similar to the monomeric yeast and
                     human topo I. Topo I enzymes are divided into: topo type
                     IA (bacterial) and type IB (eukaryotic); cd03488"
                     /db_xref="CDD:239570"
     Site            order(218,268,358..359,364,366,369,376,412..414,427..428,
                     430)
                     /site_type="DNA binding"
                     /db_xref="CDD:239570"
     Site            282
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P11387; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            318
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Region          362..739
                     /region_name="TOPEUc"
                     /note="DNA Topoisomerase I (eukaryota); smart00435"
                     /db_xref="CDD:214661"
     Site            366
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            366
                     /site_type="active"
                     /note="topo1 poison binding [active]"
                     /db_xref="CDD:239570"
     Site            414
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Region          427..428
                     /region_name="Interaction with DNA. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            445
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Region          490..495
                     /region_name="Interaction with DNA. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            503
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            508
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by CK2.
                     /evidence=ECO:0000250|UniProtKB:P11387; propagated from
                     UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            534
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            576
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Region          587..589
                     /region_name="Interaction with DNA. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            634
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     Site            652
                     /site_type="other"
                     /note="Interaction with DNA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9WUL0.1)"
     CDS             1..767
                     /gene="Top1"
                     /gene_synonym="Ab2-086"
                     /coded_by="NM_022615.2:190..2493"
                     /db_xref="GeneID:64550"
                     /db_xref="RGD:621246"
ORIGIN      
        1 msgdhlhnds qieadfrlnd shkhkdkhkd rehrhkehkk dkdkdreksk hsnsehkdse
       61 kkhkekektk hkdgssdkhk dkhkdrdkek rkeekiraag dakikkeken gfsspprikd
      121 epeddgyfap pkedikplkr prdeddadyk pkkiktedik kekkrkleee edgklkkpkn
      181 kdkdkkvaep dnkkkkakke eeqkwkwwee erypegikwk flehkgpvfa ppyeplpegv
      241 kfyydgkvmk lspkaeevat ffakmldhey ttkeifrknf fkdwrkemtn dekntitnls
      301 kcdftqmsqy fkaqsearkq mskeeklkik eenekllkey gfcvmdnhre rianfkiepp
      361 glfrgrgnhp kmgmlkrrim pediiincsk dakvpspppg hkwkevrhdn kvtwlvswte
      421 niqgsikyim lnpssrikge kdwqkyetar rlkkcvdkir nqyredwksk emkvrqrava
      481 lyfidklalr agnekeeget adtvgccslr vehinlhpel dgqeyvvefd fpgkdsiryy
      541 nkvpvekrvf knlqlfmenk qpeddlfdrl ntgilnkhlq dlmegltakv frtynasitl
      601 qqqlkeltap denvpakils ynranravai lcnhqrappk tfeksmmnlq skidakkdql
      661 adarkdlksa kadakvmkda ktkkvveskk kavqrleeql mklevqatdr eenkqialgt
      721 sklnyldpri tvawckkwgv piekiynktq rekfawaidm tdedyef
//
