LOCUS       NM_022592               2086 bp    mRNA    linear   ROD 20-MAR-2025
DEFINITION  Rattus norvegicus transketolase (Tkt), transcript variant 4, mRNA.
ACCESSION   NM_022592
VERSION     NM_022592.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Zhao,Y., Wu,Y., Hu,H., Cai,J., Ning,M., Ni,X. and Zhong,C.
  TITLE     Downregulation of transketolase activity is related to inhibition
            of hippocampal progenitor cell proliferation induced by thiamine
            deficiency
  JOURNAL   Biomed Res Int 2014, 572915 (2014)
   PUBMED   25028661
  REMARK    GeneRIF: results suggest that modulation of transketolase activity
            might be one of the mechanisms by which thiamine regulates the
            proliferation of hippocampal progenitor cells.
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   3  (bases 1 to 2086)
  AUTHORS   Mitschke,L., Parthier,C., Schroder-Tittmann,K., Coy,J., Ludtke,S.
            and Tittmann,K.
  TITLE     The crystal structure of human transketolase and new insights into
            its mode of action
  JOURNAL   J Biol Chem 285 (41), 31559-31570 (2010)
   PUBMED   20667822
REFERENCE   4  (bases 1 to 2086)
  AUTHORS   Kesimer,M., Scull,M., Brighton,B., DeMaria,G., Burns,K., O'Neal,W.,
            Pickles,R.J. and Sheehan,J.K.
  TITLE     Characterization of exosome-like vesicles released from human
            tracheobronchial ciliated epithelium: a possible role in innate
            defense
  JOURNAL   FASEB J 23 (6), 1858-1868 (2009)
   PUBMED   19190083
REFERENCE   5  (bases 1 to 2086)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   6  (bases 1 to 2086)
  AUTHORS   Wang,J.J., Martin,P.R. and Singleton,C.K.
  TITLE     Aspartate 155 of human transketolase is essential for thiamine
            diphosphate-magnesium binding, and cofactor binding is required for
            dimer formation
  JOURNAL   Biochim Biophys Acta 1341 (2), 165-172 (1997)
   PUBMED   9357955
REFERENCE   7  (bases 1 to 2086)
  AUTHORS   McCool,B.A., Plonk,S.G., Martin,P.R. and Singleton,C.K.
  TITLE     Cloning of human transketolase cDNAs and comparison of the
            nucleotide sequence of the coding region in Wernicke-Korsakoff and
            non-Wernicke-Korsakoff individuals
  JOURNAL   J Biol Chem 268 (2), 1397-1404 (1993)
   PUBMED   8419340
REFERENCE   8  (bases 1 to 2086)
  AUTHORS   Abedinia,M., Layfield,R., Jones,S.M., Nixon,P.F. and Mattick,J.S.
  TITLE     Nucleotide and predicted amino acid sequence of a cDNA clone
            encoding part of human transketolase
  JOURNAL   Biochem Biophys Res Commun 183 (3), 1159-1166 (1992)
   PUBMED   1567394
REFERENCE   9  (bases 1 to 2086)
  AUTHORS   Bublitz,C. and Steavenson,S.
  TITLE     The pentose phosphate pathway in the endoplasmic reticulum
  JOURNAL   J Biol Chem 263 (26), 12849-12853 (1988)
   PUBMED   2843500
REFERENCE   10 (bases 1 to 2086)
  AUTHORS   Casazza,J.P. and Veech,R.L.
  TITLE     The interdependence of glycolytic and pentose cycle intermediates
            in ad libitum fed rats
  JOURNAL   J Biol Chem 261 (2), 690-698 (1986)
   PUBMED   3079759
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Nov 26, 2020 this sequence version replaced NM_022592.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U09256.1, SRR26643295.51883.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                JAXUCZ010000016.1  5730232-5730308
            78-210              JAXUCZ010000016.1  5730932-5731064
            211-328             JAXUCZ010000016.1  5740422-5740539
            329-442             JAXUCZ010000016.1  5741405-5741518
            443-540             JAXUCZ010000016.1  5742133-5742230
            541-732             JAXUCZ010000016.1  5747104-5747295
            733-851             JAXUCZ010000016.1  5748625-5748743
            852-1045            JAXUCZ010000016.1  5749659-5749852
            1046-1210           JAXUCZ010000016.1  5750484-5750648
            1211-1367           JAXUCZ010000016.1  5751274-5751430
            1368-1498           JAXUCZ010000016.1  5751544-5751674
            1499-1582           JAXUCZ010000016.1  5752336-5752419
            1583-1676           JAXUCZ010000016.1  5752527-5752620
            1677-1799           JAXUCZ010000016.1  5753474-5753596
            1800-2086           JAXUCZ010000016.1  5754882-5755168
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p16"
     gene            1..2086
                     /gene="Tkt"
                     /note="transketolase"
                     /db_xref="GeneID:64524"
                     /db_xref="RGD:621036"
     exon            1..77
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1975
                     /gene="Tkt"
                     /EC_number="2.2.1.1"
                     /note="isoform 4 is encoded by transcript variant 4; TK"
                     /codon_start=1
                     /product="transketolase isoform 4"
                     /protein_id="NP_072114.2"
                     /db_xref="GeneID:64524"
                     /db_xref="RGD:621036"
                     /translation="MDPVRQIQCDREGKRFPFLSAPPASTSSPDRAMEGYHKPDQQKL
                     QALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRNPHNDR
                     FVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQG
                     LGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDINR
                     LGQSDPAPLQHQVDVYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFK
                     GRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIR
                     MPTPPNYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFI
                     ECYIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCG
                     VSIGEDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPE
                     NAIIYSNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAEMLKKEKIGVRVLDP
                     FTIKPLDKKLILDCARATKGRILTVEDHYYEGGIGEAVSAAVVGEPGVTVTRLAVSQV
                     PRSGKPAELLKMFGIDKDAIVQAVKGLVTKG"
     exon            78..210
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            211..328
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            329..442
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            443..540
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            541..732
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            733..851
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1045
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1210
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1367
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1498
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1582
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1676
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1799
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..2086
                     /gene="Tkt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaatgtcatg gatccagtga gacagatcca gtgcgaccgt gaaggcaaac gctttccgtt
       61 cctctcagct ccaccagctt ccacgtcctc gcccgaccgc gccatggagg gttaccataa
      121 gccagatcag cagaagctcc aggccctgaa ggacacagcc aatcgcctgc gcatcagctc
      181 catccaggcc accaccgcgg caggctcggg acaccccaca tcatgctgca gcgctgccga
      241 gatcatggct gtcctgtttt tccataccat gcgctacaag gccctggatc cccgaaaccc
      301 tcacaatgat cgctttgtgc tctccaaggg ccatgcagct cccatcttat atgcagtctg
      361 ggctgaagct ggcttcctgc ctgaggcaga gctgctgaac ctgaggaaaa tcagctctga
      421 cttggatggg catcctgtcc cgaaacaagc cttcaccgat gtggccactg gctccctggg
      481 ccaggggctg ggagctgcgt gcgggatggc atacacaggc aaatacttcg acaaagccag
      541 ctaccgagtc tattgcatgc tgggagacgg ggaggtgtcc gagggctccg tttgggaggc
      601 catggccttc gctggaattt acaagctgga caacctcgtt gccatttttg acatcaaccg
      661 tctgggccag agcgacccag ccccgctgca gcaccaagtg gacgtctacc agaagcgctg
      721 tgaggccttt ggctggcatg ccatcatcgt ggatgggcac agtgtggagg agctgtgcaa
      781 ggcctttggt caggccaagc accaaccaac agccatcatt gccaagacct tcaagggccg
      841 cgggatcaca gggattgaag acaaggaggc gtggcatggg aagcccctcc ccaaaaacat
      901 ggctgagcag attatccagg agatttacag ccaggttcag agcaaaaaga agatcctcgc
      961 cacgccccct caggaggatg ccccttccgt ggacattgcc aacatccgaa tgcctacccc
     1021 acccaactac aaagtggggg acaagatagc cacacggaaa gcctatggat tggcccttgc
     1081 caagctgggc cacgccagtg accgcatcat cgccctggat ggagacacca aaaattccac
     1141 cttctcagag ctcttcaaaa aggagcaccc agaccgtttc atcgagtgct acattgctga
     1201 gcagaacatg gtgagcattg ctgtgggctg tgccacacgt gacaggacag tgcccttctg
     1261 cagcactttt gcggccttct tcacacgcgc cttcgaccag atccgcatgg ccgccatctc
     1321 cgagagcaac atcaacctct gtggctccca ttgcggcgtg tccattgggg aagacgggcc
     1381 ctcgcagatg gccctggaag acctggccat gtttcggtcg gtccctatgt ccaccgtctt
     1441 ttacccaagt gatggagttg ccacagagaa ggcagtggaa ttagcagcca atacaaaggg
     1501 catctgcttc attcggacca gccgcccaga aaatgccatt atctatagca acaacgagga
     1561 tttccaggtt ggccaagcca aggtggtcct gaagagcaag gacgaccaag tgacggtgat
     1621 cggggctggc gtaactctgc acgaggctct ggctgctgca gagatgttga agaaagagaa
     1681 gatcggcgtc cgtgtactgg accccttcac catcaagccc ctggacaaaa agctcattct
     1741 cgactgtgcc agagcaacca aaggcaggat cctcaccgtg gaggaccact actatgaagg
     1801 tggcataggc gaggcagtat ctgctgcggt agtgggcgaa cctggagtca cagtcactcg
     1861 cctggcggtc agccaagtac cacgaagtgg gaagccagct gagctgctga agatgtttgg
     1921 tattgacaaa gacgccattg tgcaagctgt gaagggcctt gtcaccaagg gctaggaagg
     1981 acatgggatg ccgggtgggt gaactacaca ttccagggat gttctggcaa aggtgctcaa
     2041 gggtgtaccg agtggaaagg taaatatatg ttttgagaaa aaaatt
//
