LOCUS       NM_001106166            2389 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus thymidine kinase 2 (Tk2), mRNA.
ACCESSION   NM_001106166 XM_001057449 XM_226211
VERSION     NM_001106166.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wang,L., Sun,R. and Eriksson,S.
  TITLE     Basic biochemical characterization of cytosolic enzymes in
            thymidine nucleotide synthesis in adult rat tissues: implications
            for tissue specific mitochondrial DNA depletion and
            deoxynucleoside-based therapy for TK2-deficiency
  JOURNAL   BMC Mol Cell Biol 21 (1), 33 (2020)
   PUBMED   32345222
  REMARK    GeneRIF: Basic biochemical characterization of cytosolic enzymes in
            thymidine nucleotide synthesis in adult rat tissues: implications
            for tissue specific mitochondrial DNA depletion and
            deoxynucleoside-based therapy for TK2-deficiency.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wang,L., Sun,R. and Eriksson,S.
  TITLE     The kinetic effects on thymidine kinase 2 by enzyme-bound dTTP may
            explain the mitochondrial side effects of antiviral thymidine
            analogs
  JOURNAL   Antimicrob Agents Chemother 55 (6), 2552-2558 (2011)
   PUBMED   21444706
REFERENCE   3  (bases 1 to 2389)
  AUTHORS   Wang,L. and Eriksson,S.
  TITLE     Tissue specific distribution of pyrimidine deoxynucleoside salvage
            enzymes shed light on the mechanism of mitochondrial DNA depletion
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 29 (4-6), 400-403 (2010)
   PUBMED   20544526
  REMARK    GeneRIF: A cytosolic form of TK2 was identified in all tested
            tissues in addition to mitochondrial TK2. TK1 was detected in liver
            and spleen cytosolic extracts while dCK was found in liver, spleen
            and lung cytosolic extracts.
REFERENCE   4  (bases 1 to 2389)
  AUTHORS   Akman,H.O., Dorado,B., Lopez,L.C., Garcia-Cazorla,A., Vila,M.R.,
            Tanabe,L.M., Dauer,W.T., Bonilla,E., Tanji,K. and Hirano,M.
  TITLE     Thymidine kinase 2 (H126N) knockin mice show the essential role of
            balanced deoxynucleotide pools for mitochondrial DNA maintenance
  JOURNAL   Hum Mol Genet 17 (16), 2433-2440 (2008)
   PUBMED   18467430
REFERENCE   5  (bases 1 to 2389)
  AUTHORS   Zhou,X., Solaroli,N., Bjerke,M., Stewart,J.B., Rozell,B.,
            Johansson,M. and Karlsson,A.
  TITLE     Progressive loss of mitochondrial DNA in thymidine kinase
            2-deficient mice
  JOURNAL   Hum Mol Genet 17 (15), 2329-2335 (2008)
   PUBMED   18434326
REFERENCE   6  (bases 1 to 2389)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2389)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   8  (bases 1 to 2389)
  AUTHORS   Mancuso,M., Filosto,M., Bonilla,E., Hirano,M., Shanske,S., Vu,T.H.
            and DiMauro,S.
  TITLE     Mitochondrial myopathy of childhood associated with mitochondrial
            DNA depletion and a homozygous mutation (T77M) in the TK2 gene
  JOURNAL   Arch Neurol 60 (7), 1007-1009 (2003)
   PUBMED   12873860
REFERENCE   9  (bases 1 to 2389)
  AUTHORS   Wettin,K., Johansson,M., Zheng,X., Zhu,C. and Karlsson,A.
  TITLE     Cloning of mouse mitochondrial thymidine kinase 2 cDNA
  JOURNAL   FEBS Lett 460 (1), 103-106 (1999)
   PUBMED   10571069
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474006.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_226211.4, XM_001057449.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132266 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19p14"
     gene            1..2389
                     /gene="Tk2"
                     /note="thymidine kinase 2"
                     /db_xref="GeneID:291824"
                     /db_xref="RGD:1309279"
     exon            1..148
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..828
                     /gene="Tk2"
                     /EC_number="2.7.1.21"
                     /note="thymidine kinase 2, mitochondrial"
                     /codon_start=1
                     /product="thymidine kinase 2"
                     /protein_id="NP_001099636.1"
                     /db_xref="GeneID:291824"
                     /db_xref="RGD:1309279"
                     /translation="MLLRSLRSWAARSLRSMGPGSSGSPGSLDSGAGPLWAPRRACPP
                     DKDREKDKEKKAVVCIEGNIASGKTTCLEFFSNTTDVEVLMEPVLKWRNVHGHNPLSL
                     MYHNASRWGLTLQTYVQLTMLDQHTRPQMSPVRLMERSIYSARYIFVENLYRSGKMPE
                     VDYAILSEWFDWIIKNIDVSVDLIVYLRTTPEICYQRLKMRCREEEKVIPVEYLSAIH
                     HLYEEWLVTGSLFPAAAPVLVIEADHNLEKMLELFEQNRARILTPENWKHGP"
     exon            149..186
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            187..261
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            262..315
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            316..405
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            406..479
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            480..568
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            569..648
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..729
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..2389
                     /gene="Tk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 catgaaccag aagcaatgct gctgcggtcg ctccgtagct gggctgcccg gtcgctacgc
       61 agcatgggcc cggggagctc agggagcccc gggagtctcg attcgggcgc tgggccgctg
      121 tgggcacctc gccgcgcctg tcctccggat aaagatagag aaaaagataa ggagaaaaaa
      181 gcagtggttt gtattgaggg caatattgca agtggaaaga cgacgtgcct ggagtttttc
      241 tccaacacaa cagacgtcga ggtgttaatg gagcctgtgc tcaagtggag aaatgtccac
      301 ggtcataacc ctctgagtct catgtaccat aatgccagtc ggtggggcct cacgctgcag
      361 acatatgtgc agctcaccat gctggaccag cacactcgtc ctcagatgtc acctgtacgg
      421 ttgatggaaa ggtcaattta cagcgcaaga tacatttttg tagaaaacct gtatagaagt
      481 gggaagatgc ccgaggttga ctatgcgatt ctgtctgagt ggtttgactg gatcatcaag
      541 aacattgatg tctctgttga tctgatagtt tatcttcgaa ccactcctga aatctgctac
      601 cagagattaa agatgaggtg ccgggaagag gagaaagtca ttccggtgga atacctcagt
      661 gctattcacc acctctacga ggagtggctg gtcactggta gccttttccc agctgcagcc
      721 cctgttctgg tgattgaggc tgaccacaac ttggagaaaa tgttagaact ctttgagcaa
      781 aaccgggccc ggatattaac tccagagaat tggaagcatg ggccttagga ctgagcggtg
      841 tacaagccag tgtcaggaac agcccaggca gggccaggtc agctgctagg aacacttgga
      901 agaatctact ctcaaaaggg tctgtatctg atcagatctg ttttcacaat ggtcttttca
      961 ttgtagtaca aaccgctttt gtctgtcaga tccttgcaat atactttccc cgaggtcccc
     1021 tcctgacgct gaagctctgt gtaaaaacac ttgctgtatc cttgggggtt gttctggttc
     1081 ctatgcatca ggtcaccttg tggggtgagg gagcagtctc atcccaggta aggatttgaa
     1141 acccagagag gtgcagggac tcttcaaggt cacaaggcag gacatagtgg ggctgggacc
     1201 attgagatac ctggcttggg ctgcctcctc ctaagcagtt cccctcagcc cttctgttct
     1261 gagcgcccgc cggcccccac ctctgtgaga agagctggca gtttgtggat tctgtcatct
     1321 ttcggtaccg gacaagtttc aaggagacca gacatcacta ggcattggca cacctcttat
     1381 ttggcatgag cccagggttt cctggcatga aggtactgta acctctcttc ctttgtcatc
     1441 tggccctcag tgtccactgg ggaatcctgg accagtcttt gcacggtgcc agtgtccttg
     1501 aagatgaggt ctttcccaga ctctgccagt ttcatgtcat tctatgtcag aaaaaaaggg
     1561 cttatgggac ctggacactt taccctgtgc cgagggaggc caaaggcagc cagctgggct
     1621 tccaaggccc catcgctttc tcctgtcctt gtgtgaggac cagtgccctt gctccagtga
     1681 gagtgacaat ggccttaact cctttgaagt gttttcccat ctgagtggat gtcacggacc
     1741 acagagcatg aagtcatttc tattggatca gaaaatacct tagctttgct tggccataac
     1801 tgcaccaaag gtgcagctgt tacttgaacc agtcgagacc aagaaaaaga ccgacattcc
     1861 ctggacagtg agatggaaag tcatggctct ccaacaaagg catttaaggc cttgctgctc
     1921 ttgacattct gggcagatgg agcatctgga gaagactgga cccagatgat agaaggggac
     1981 gataactgta ctctggagag acacagcgtg gactgggcgg ggctgcagat gcatgtgtcc
     2041 agctggctct gctctgctct gctctgcctg tctgtaggtg cctgggatgc ctgcctggta
     2101 gctagaaaga gggccaagag tccagctcgt ggcctttcct gtcttggact tttctgttaa
     2161 tatgaagaag aatagcagcc agggggattg tttaccagca cctgcagtct agcaatggat
     2221 aaaccgtaaa cctgggaaag gcaggtttga accagcagtc ctgatggggc atctttagaa
     2281 ttagcaaagg aacagtccag tacttgcccc tttgcttaac cagagcacct gttttgggaa
     2341 gaaatgtgat gtttctcttg ctctatggta gcaaataaat tctgcagct
//
