LOCUS       NM_052800               2263 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus thymidine kinase 1 (Tk1), mRNA.
ACCESSION   NM_052800 XM_001081763
VERSION     NM_052800.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Mutahir,Z., Clausen,A.R., Andersson,K.M., Wisen,S.M.,
            Munch-Petersen,B. and Piskur,J.
  TITLE     Thymidine kinase 1 regulatory fine-tuning through tetramer
            formation
  JOURNAL   FEBS J 280 (6), 1531-1541 (2013)
   PUBMED   23351158
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Skovgaard,T., Uhlin,U. and Munch-Petersen,B.
  TITLE     Comparative active-site mutation study of human and Caenorhabditis
            elegans thymidine kinase 1
  JOURNAL   FEBS J 279 (10), 1777-1787 (2012)
   PUBMED   22385435
REFERENCE   3  (bases 1 to 2263)
  AUTHORS   Vinayagam,A., Stelzl,U., Foulle,R., Plassmann,S., Zenkner,M.,
            Timm,J., Assmus,H.E., Andrade-Navarro,M.A. and Wanker,E.E.
  TITLE     A directed protein interaction network for investigating
            intracellular signal transduction
  JOURNAL   Sci Signal 4 (189), rs8 (2011)
   PUBMED   21900206
REFERENCE   4  (bases 1 to 2263)
  AUTHORS   Wang,L. and Eriksson,S.
  TITLE     Tissue specific distribution of pyrimidine deoxynucleoside salvage
            enzymes shed light on the mechanism of mitochondrial DNA depletion
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 29 (4-6), 400-403 (2010)
   PUBMED   20544526
  REMARK    GeneRIF: A cytosolic form of TK2 was identified in all tested
            tissues in addition to mitochondrial TK2. TK1 was detected in liver
            and spleen cytosolic extracts while dCK was found in liver, spleen
            and lung cytosolic extracts.
REFERENCE   5  (bases 1 to 2263)
  AUTHORS   Mirzaee,S., Eriksson,S. and Albertioni,F.
  TITLE     Differences in cytosolic and mitochondrial 5'-nucleotidase and
            deoxynucleoside kinase activities in Sprague-Dawley rat and CD-1
            mouse tissues: implication for the toxicity of nucleoside analogs
            in animal models
  JOURNAL   Toxicology 267 (1-3), 159-164 (2010)
   PUBMED   19913594
REFERENCE   6  (bases 1 to 2263)
  AUTHORS   Arcot,S.S., Traina-Dorge,V.L. and Deininger,P.L.
  TITLE     The rat thymidine kinase gene 5' region: evolution of a promoter
  JOURNAL   DNA Seq 2 (2), 129-131 (1991)
   PUBMED   1777676
REFERENCE   7  (bases 1 to 2263)
  AUTHORS   Murphy,A.J. and Efstratiadis,A.
  TITLE     Cloning vectors for expression of cDNA libraries in mammalian cells
  JOURNAL   Proc Natl Acad Sci U S A 84 (23), 8277-8281 (1987)
   PUBMED   3479791
REFERENCE   8  (bases 1 to 2263)
  AUTHORS   Sakai,K., Murata,N. and Yamane,Y.
  TITLE     Effect of copper and cadmium pretreatments on DNA synthesis and
            thymidine kinase activity in the liver of
            dimethylnitrosamine-treated and partially hepatectomized rats
  JOURNAL   Gan 74 (5), 663-670 (1983)
   PUBMED   6642140
REFERENCE   9  (bases 1 to 2263)
  AUTHORS   Kozak,C.A. and Ruddle,F.H.
  TITLE     Assignment of the genes for thymidine kinase and galactokinase to
            Mus musculus chromosome 11 and the preferential segregation of this
            chromosome in Chinese hamster/mouse somatic cell hybrids
  JOURNAL   Somatic Cell Genet 3 (2), 121-133 (1977)
   PUBMED   204065
REFERENCE   10 (bases 1 to 2263)
  AUTHORS   Herzfeld,A., Rosenoer,V.M. and Raper,S.M.
  TITLE     Glutamate dehydrogenase, alanine aminotransferase, thymidine
            kinase, and arginase in fetal and adult human and rat liver
  JOURNAL   Pediatr Res 10 (12), 960-964 (1976)
   PUBMED   995499
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK471539.1, M22642.1, CV072953.1, EV770793.1, AW919734.1,
            BF547747.1 and BP495650.1.
            
            On Oct 13, 2007 this sequence version replaced XM_001081763.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ229684.1, EV773050.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760476, SAMEA5760494
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 CK471539.1         1-6
            7-425               M22642.1           4-422
            426-773             CV072953.1         425-772
            774-1365            EV770793.1         89-680
            1366-1485           AW919734.1         531-650
            1486-1702           BF547747.1         50-266
            1703-2263           BP495650.1         1-561               c
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.2"
     gene            1..2263
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /note="thymidine kinase 1"
                     /db_xref="GeneID:24834"
                     /db_xref="RGD:621014"
     exon            1..128
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..761
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /EC_number="2.7.1.21"
                     /note="thymidine kinase 1, soluble"
                     /codon_start=1
                     /product="thymidine kinase, cytosolic"
                     /protein_id="NP_434687.1"
                     /db_xref="GeneID:24834"
                     /db_xref="RGD:621014"
                     /translation="MSYINLPTVLPISPSKTRGQIQVILGPMFSGKSTELMRRVRRFQ
                     IAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLKDVAQEALGVAVIGIDEGQFF
                     PDIVDFCETMANTGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREA
                     SYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTADNKENYSVLGQPIEIPAVRK
                     LFAPQQILQCNSAN"
     exon            129..160
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     exon            161..271
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     exon            272..365
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     exon            366..455
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     exon            456..575
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
     exon            576..2263
                     /gene="Tk1"
                     /gene_synonym="Tk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgttgtgtg tgactaacgt actccactca aggacccggg tgctaggatt taccccatcg
       61 ccatgagcta catcaacctg cccaccgtgc tgcccatctc ccccagcaag acccgggggc
      121 agatccaggt gattctcggg cccatgttct cagggaaaag cacggagctg atgaggagag
      181 taaggcgctt ccagatcgcc cagtacaagt gcctggtcat caagtatgcc aaagacacgc
      241 gctatagcaa cagcttctcc acacatgacc ggaacaccat ggatgcactg ccggcctgca
      301 tgctcaagga tgtggcccag gaggccttgg gtgtggctgt cattggcatc gatgaagggc
      361 agtttttccc tgacattgtg gatttctgtg aaacgatggc caatacaggc aagacagtga
      421 ttgtggcggc gctggacgga accttccaga ggaaggcttt cggcagcatc ttgaacctgg
      481 tgcccttggc cgagagtgtg gtgaagctca ccgctgtgtg catggagtgc tttcgagaag
      541 cttcctacac caagaggctg ggcctggaga aagaggtgga ggtgattggc ggagcggaca
      601 agtaccactc tgtttgccgc ctgtgctact ttaagaagtc ttcagcccag actgcagaca
      661 acaaagagaa ctactcagtg ctggggcagc ctatagagat cccggctgtc aggaagctct
      721 ttgcccctca gcaaatccta cagtgtaact ctgccaactg aggggacccg gggcctgcca
      781 gctccttccc caggttggac tcccagagag cagggaaagg ggcggggcct gccattccta
      841 ctggacattg caccttggct tatctgcttg ttgaaacctt tcttagttcc cttcttataa
      901 tggttgtgag cccccaaccc aacgcttctc tcttaaaccc cgtgattcag gcaacatcat
      961 actttactgg tcttgcttca ctagagagct gcatggggag accttgctgg aaattgaact
     1021 tgggacctct ggaagagtgg tcagtcctct taacccctga gctatcattc cagcaccttt
     1081 agctcccttc ttggttgcca agacacctca gcacagactg gagcccagac cctgcctggt
     1141 ggctcgggtc tcacattcag ccaatggtga gaacagagcc acccagcatt gtgacattgg
     1201 tggtgctagc ttcttccttg ttggtggctt tcactgctgg gtttccgttt cagccccaca
     1261 ctttaccaag gctctagcct tctcacagct ctcccatata tgtatccctt gacccctact
     1321 gccattatag ctccaatcct tctctgctca gctctcagta agccaaggga gtatgtttct
     1381 tgtgccccat tacttataac ttacttagcc tgggtttccc taaatcttgc aactagcacc
     1441 cacatgactt gggggtagaa atttcttctg tagtactaat ggtctccacc acagcccaag
     1501 aggaacagtc acccacattt ctatctggtt ggtttgggga caaaaatggc aaaggcacct
     1561 gccaggagag tcaggagatg gtgtttctag ctggcacgga aggtcaggtt ttgtgagacc
     1621 gatacctgat agattgagtt gaggaaacgg ctcagatggc cttggaaagt gggcagggtg
     1681 acacttgagg accagagacc tgggtctaag gtgtggggtg ctgctggtgc catgcccaac
     1741 tgctcaaggc atgctctcca gaggaggccc acagctcgga gcccggcctg tcagtttata
     1801 cttcagattt tgaaagacgc ggccagcggc tctccacagc cccattattg cacaagagtt
     1861 tacctcaggg gcatggctgc ttcctgacag agatgggtcc actccctgcc aagtccctgg
     1921 aaggtgtcaa gaggggccag agtcaacctc agaggggcaa cacccagggg cccctggcag
     1981 gcatcggaaa cctggccagc tgtccagaac aatccgcctc acaagctgtc atggtggctg
     2041 ttttaggatg cagtagaagg cagtgtgaag actgagaccc cactagggaa gctgccaccc
     2101 caaacttaaa gaacatggtc taactgggca tggtggtctg cagctttacc cagtactcaa
     2161 accacatgtt ggctcccaac catctgtaag gggattcaat gcctcctctt gtgtggatat
     2221 aaataaaaca aaaataaatc ttaaaaaaac aaaacaaaac aaa
//
