LOCUS       NM_001401677            1603 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus translocase of inner mitochondrial membrane 50
            (Timm50), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001401677 XM_003748806
VERSION     NM_001401677.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Wenz,L.S., Opalinski,L., Wiedemann,N. and Becker,T.
  TITLE     Cooperation of protein machineries in mitochondrial protein sorting
  JOURNAL   Biochim Biophys Acta 1853 (5), 1119-1129 (2015)
   PUBMED   25633533
  REMARK    Review article
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Schulz,C., Schendzielorz,A. and Rehling,P.
  TITLE     Unlocking the presequence import pathway
  JOURNAL   Trends Cell Biol 25 (5), 265-275 (2015)
   PUBMED   25542066
  REMARK    Review article
REFERENCE   3  (bases 1 to 1603)
  AUTHORS   Xu,H., Somers,Z.B., Robinson,M.L. 2nd and Hebert,M.D.
  TITLE     Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts
            with proteins involved in snRNP biogenesis
  JOURNAL   BMC Cell Biol 6 (1), 29 (2005)
   PUBMED   16008839
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1603)
  AUTHORS   Guo,Y., Cheong,N., Zhang,Z., De Rose,R., Deng,Y., Farber,S.A.,
            Fernandes-Alnemri,T. and Alnemri,E.S.
  TITLE     Tim50, a component of the mitochondrial translocator, regulates
            mitochondrial integrity and cell death
  JOURNAL   J Biol Chem 279 (23), 24813-24825 (2004)
   PUBMED   15044455
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 17, 2022 this sequence version replaced XM_003748806.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.2116.1,
                                           SRR26360194.658289.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               JAXUCZ010000001.1  92710176-92710311   c
            137-287             JAXUCZ010000001.1  92709010-92709160   c
            288-319             JAXUCZ010000001.1  92708514-92708545   c
            320-341             JAXUCZ010000001.1  92708307-92708328   c
            342-400             JAXUCZ010000001.1  92705753-92705811   c
            401-520             JAXUCZ010000001.1  92705546-92705665   c
            521-625             JAXUCZ010000001.1  92705139-92705243   c
            626-724             JAXUCZ010000001.1  92704951-92705049   c
            725-881             JAXUCZ010000001.1  92704200-92704356   c
            882-988             JAXUCZ010000001.1  92703800-92703906   c
            989-1603            JAXUCZ010000001.1  92702437-92703051   c
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1603
                     /gene="Timm50"
                     /note="translocase of inner mitochondrial membrane 50"
                     /db_xref="GeneID:687295"
                     /db_xref="RGD:1587684"
     exon            1..136
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1090
                     /gene="Timm50"
                     /note="translocase of inner mitochondrial membrane 50
                     homolog"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit TIM50"
                     /protein_id="NP_001388606.1"
                     /db_xref="GeneID:687295"
                     /db_xref="RGD:1587684"
                     /translation="MAASAALFSRLRSGLRVGARGLCTRLAPPPPRTPEQVTEIANRG
                     GSKTQGPQHQPGSEGPSYAKKVALWIAGLLGAGGTVSIVYIFGNNPVDENGTKIPDEF
                     DSDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLH
                     PEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRL
                     FRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVALRPWDGNSDDRVL
                     LDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNK
                     QGLFFGSLTSRLWPRSKQP"
     exon            137..287
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            288..319
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            320..341
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            342..400
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            401..520
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            521..625
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            626..724
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            725..881
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            882..988
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1603
                     /gene="Timm50"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggcggggcc gcgtggcgtc agcgcaagat ggcggcctcg gcagctctct tctcgcgctt
       61 gcggagcggg ctccgggtag gcgcgcgggg actgtgcacg cggctggcgc cgccacctcc
      121 ccgcactcca gaacaggtta ctgagatcgc caaccgtggg ggctccaaga cccaagggcc
      181 acagcatcag ccaggctcag agggtcccag ctatgccaaa aaagttgcac tgtggattgc
      241 tggtttgctt ggggctggtg ggactgtcag catcgtctac atttttggaa acaaccccgt
      301 ggatgaaaat ggcaccaaga ttcctgatga attcgacagc gatccaattc tggttcagca
      361 gttgcgccgg acatacaaat acttcaagga ttacagacag atgatcatcg agcccaccag
      421 tccctgcctt ctcccagacc ctctgcggga gccctactac cagccacctt atacactggt
      481 cctggagctc accggggtcc ttctgcatcc agagtggtcg ctggccaccg gctggaggtt
      541 taagaagcgc ccaggcatcg agaccttgtt ccagcagctt gcccctctgt atgaaatagt
      601 catcttcaca tcagagactg gcatgactgc atttccactc attgatagtg tggaccccca
      661 cggcttcatc tcttaccgtc tgttccggga tgccaccaga tatatggagg gacaccatgt
      721 gaaggacatc tcgtgtctga atcgggaccc agcccgagta gtagtcgtgg actgcaagaa
      781 ggaagctttc cgcctacagc ccttcaatgg tgtcgccttg cggccctggg atggcaactc
      841 agatgaccgg gtcttgctgg acctgtctgc cttcctcaag accatcgcgc tgaaccaagt
      901 agaggacgtc cggactgtgc tggagcacta tgctctagag gatgacccat tggaggcatt
      961 caaacagcgg cagagccggc ttgaacagga ggagcagcag cgcctggcgg agctctccaa
     1021 gtccaacaag cagggcctct tctttggctc actcaccagc cgcctgtggc ctcgatccaa
     1081 gcagccctga ctactaggct tcctcagaga cttggtgcct gggcctcagg ccccagccct
     1141 cagagcaagg tttggacagc tatgtgtcca ataaagcgag tcccagatgc cacaatgcct
     1201 gtgtccagag agtccagatg gggcgctggg gtgaggtctt ggactcggtc atcattcaca
     1261 gtgactgagc accaccacta ggctgggcac cgtgagtgcc tcgttggagt ggggctcctg
     1321 ccagcctgca actgaaaacc cgggtttctg ctgcacacgt cttcccgtat actccatgag
     1381 ctataaagaa aggcctctct ctctctctct ctctctctct ttctctctct ctctccctct
     1441 ctcctctccc ttctctcctc tcccctctcc tctttccctt tctcttctct cctctctccc
     1501 tccctcctac cctccctccc gccttggctc tggactcctt gtgtgctgtg gaaataaaga
     1561 gaaatatggc ctctggggag ccgcctcaag ggctgtcagc cta
//
