LOCUS       NM_172075               2408 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus thimet oligopeptidase 1 (Thop1), mRNA.
ACCESSION   NM_172075
VERSION     NM_172075.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Woitowich,N.C., Philibert,K.D., Leitermann,R.J., Wungjiranirun,M.,
            Urban,J.H. and Glucksman,M.J.
  TITLE     EP24.15 as a Potential Regulator of Kisspeptin Within the
            Neuroendocrine Hypothalamus
  JOURNAL   Endocrinology 157 (2), 820-830 (2016)
   PUBMED   26653570
  REMARK    GeneRIF: Coincubation of Kiss1 and EP24.15 demonstrated multiple
            cleavages of the peptide in vitro.  immunohistochemistry indicated
            coexpression of EP24.15 and Kiss1, implicating that the regulation
            of Kiss1 by EP24.15 could occur in vivo.
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Pereira,M.G., Souza,L.L., Becari,C., Duarte,D.A., Camacho,F.R.,
            Oliveira,J.A., Gomes,M.D., Oliveira,E.B., Salgado,M.C.,
            Garcia-Cairasco,N. and Costa-Neto,C.M.
  TITLE     Angiotensin II-independent angiotensin-(1-7) formation in rat
            hippocampus: involvement of thimet oligopeptidase
  JOURNAL   Hypertension 62 (5), 879-885 (2013)
   PUBMED   24041943
  REMARK    GeneRIF: we show that Ang-(1-7) is the main metabolite of
            angiotensin I in rat hippocampi, and, strikingly, that thimet
            oligopeptidase is the main enzyme involved in the generation of
            Ang-(1-7).
REFERENCE   3  (bases 1 to 2408)
  AUTHORS   Russo,L.C., Asega,A.F., Castro,L.M., Negraes,P.D., Cruz,L.,
            Gozzo,F.C., Ulrich,H., Camargo,A.C., Rioli,V. and Ferro,E.S.
  TITLE     Natural intracellular peptides can modulate the interactions of
            mouse brain proteins and thimet oligopeptidase with 14-3-3epsilon
            and calmodulin
  JOURNAL   Proteomics 12 (17), 2641-2655 (2012)
   PUBMED   22740335
REFERENCE   4  (bases 1 to 2408)
  AUTHORS   Dalio,F.M., Visniauskas,B., Bicocchi,E.S., Perry,J.C., Freua,R.,
            Gesteira,T.F., Nader,H.B., Machado,M.F., Tufik,S., Ferro,E.S.,
            Andersen,M.L., Toledo,C.A., Chagas,J.R. and Oliveira,V.
  TITLE     Acute cocaine treatment increases thimet oligopeptidase in the
            striatum of rat brain
  JOURNAL   Biochem Biophys Res Commun 419 (4), 724-727 (2012)
   PUBMED   22387539
  REMARK    GeneRIF: TOP changes in the brain could play important role in the
            balance of neuropeptide level correlated with cocaine effects.
REFERENCE   5  (bases 1 to 2408)
  AUTHORS   Pollio,G., Hoozemans,J.J., Andersen,C.A., Roncarati,R., Rosi,M.C.,
            van Haastert,E.S., Seredenina,T., Diamanti,D., Gotta,S.,
            Fiorentini,A., Magnoni,L., Raggiaschi,R., Rozemuller,A.J.,
            Casamenti,F., Caricasole,A. and Terstappen,G.C.
  TITLE     Increased expression of the oligopeptidase THOP1 is a
            neuroprotective response to Abeta toxicity
  JOURNAL   Neurobiol Dis 31 (1), 145-158 (2008)
   PUBMED   18571100
  REMARK    GeneRIF: increase in THOP1 expression might be part of a
            compensatory defense mechanism of the brain against an increased
            Abeta load
REFERENCE   6  (bases 1 to 2408)
  AUTHORS   McCool,S. and Pierotti,A.R.
  TITLE     Expression of the thimet oligopeptidase gene is regulated by
            positively and negatively acting elements
  JOURNAL   DNA Cell Biol 19 (12), 729-738 (2000)
   PUBMED   11177571
REFERENCE   7  (bases 1 to 2408)
  AUTHORS   Tullai,J.W., Cummins,P.M., Pabon,A., Roberts,J.L., Lopingco,M.C.,
            Shrimpton,C.N., Smith,A.I., Martignetti,J.A., Ferro,E.S. and
            Glucksman,M.J.
  TITLE     The neuropeptide processing enzyme EC 3.4.24.15 is modulated by
            protein kinase A phosphorylation
  JOURNAL   J Biol Chem 275 (47), 36514-36522 (2000)
   PUBMED   10969067
REFERENCE   8  (bases 1 to 2408)
  AUTHORS   Sleat,D.E., Sohar,I., Lackland,H., Majercak,J. and Lobel,P.
  TITLE     Rat brain contains high levels of mannose-6-phosphorylated
            glycoproteins including lysosomal enzymes and palmitoyl-protein
            thioesterase, an enzyme implicated in infantile neuronal
            lipofuscinosis
  JOURNAL   J Biol Chem 271 (32), 19191-19198 (1996)
   PUBMED   8702598
REFERENCE   9  (bases 1 to 2408)
  AUTHORS   McKie,N., Dando,P.M., Rawlings,N.D. and Barrett,A.J.
  TITLE     Thimet oligopeptidase: similarity to 'soluble angiotensin
            II-binding protein' and some corrections to the published amino
            acid sequence of the rat testis enzyme
  JOURNAL   Biochem J 295 (Pt 1) (Pt 1), 57-60 (1993)
   PUBMED   8216239
REFERENCE   10 (bases 1 to 2408)
  AUTHORS   Pierotti,A., Dong,K.W., Glucksman,M.J., Orlowski,M. and
            Roberts,J.L.
  TITLE     Molecular cloning and primary structure of rat testes
            metalloendopeptidase EC 3.4.24.15
  JOURNAL   Biochemistry 29 (45), 10323-10329 (1990)
   PUBMED   2261476
  REMARK    Erratum:[Biochemistry. 1994 Jan 18;33(2):622. doi:
            10.1021/bi00168a600.. PMID: 8286394]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB780843.1 and BC081706.1.
            
            On Mar 8, 2007 this sequence version replaced NM_172075.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081706.1, M61142.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                CB780843.1         21-93
            74-2408             BC081706.1         1-2335
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..2408
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /note="thimet oligopeptidase 1"
                     /db_xref="GeneID:64517"
                     /db_xref="RGD:68330"
     exon            1..150
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2198
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /EC_number="3.4.24.15"
                     /note="endopeptidase 24.15; PZ-peptidase;
                     endo-oligopeptidase A; soluble metallo-endopeptidase;
                     metalloendopeptidase"
                     /codon_start=1
                     /product="thimet oligopeptidase"
                     /protein_id="NP_742072.2"
                     /db_xref="GeneID:64517"
                     /db_xref="RGD:68330"
                     /translation="MKPPAACAGDVVDTVSPCSTVNHLRWDLSAQQIRALTTQLIEQT
                     KCVYDRVGAQDFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPNKDIRAASTEAD
                     KKLSEFDVEMSMRQDVYQRVVWLQEKIPKDSLKPEAARYLERLIKLGRRNGLHLPQDT
                     QEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLNSLEKTEDGKLKVT
                     LKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSNLLGFRTHA
                     DYVLEMNMAKTSQTVATFLDELARKLKPLGEQERAVILELKEAECAKRGLPFDGRIHA
                     WDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHE
                     DVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAIAAMVA
                     NFTKPTPDVPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQML
                     ENWVWEKEPLMRMSQHYRTGGEAPEDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHT
                     QTDVDPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFH
                     TRFKQEGVLSPKVGMDYRTSILRPGGSEDASTMLKQFLGRDPKQDAFLLSKGLQVEGC
                     EPPAC"
     exon            151..363
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            364..512
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            513..620
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            621..723
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            724..884
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1020
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1387
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1589
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1776
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1905
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2042
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2394
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggcggcagg cgcctcagtc tgagagccgc tgcggaggaa ccgactgagg tgaagcgagc
       61 tcccaaggag acccggaccg cacaggcgtg aggaccagag gagggcgtgc ccgtcccgcg
      121 cgcagcctgc cgccatgaag ccccccgcag cctgtgctgg ggacgtggtg gatacagtgt
      181 caccatgctc caccgtgaat catctgcgct gggacctgag cgcacagcag atcagagctc
      241 taaccacaca gctcatcgag cagaccaagt gtgtgtacga ccgtgtgggc gcccaggact
      301 tcgaggacgt gtcctatgag agcacactga aggcactggc tgacgtggag gtcacctaca
      361 cagtgcagag gaacattctc gacttccccc agcacgtgtc tccgaacaag gacatccgcg
      421 cagccagcac agaggctgac aagaagctct cagagtttga tgtggagatg agcatgaggc
      481 aggacgtgta ccagagggtc gtgtggctgc aggagaaaat cccgaaagac tcactgaagc
      541 ctgaggcagc tcgctacctg gagcggctca tcaagctggg ccggagaaac gggctccact
      601 tacctcagga cacacaggag aagatcaaga acatcaagaa gaggctgagc ctgctgtgca
      661 tcgacttcaa caagaacctg aatgaggaca ccaccttcct gcccttcacg agagaggagc
      721 tgggcgggct ccccgaggac ttcctgaact ccctggagaa gacagaggac ggcaaactga
      781 aggtcaccct caagtatcca cactatttcc cactgctgaa gaagtgccac gtgcccgaga
      841 cacgacgcct gttggaggag gccttcaact gtcgctgcaa ggaggagaac tgtgccatcc
      901 tgaaggagct agtgtccctg cgggcgcaga agtccaacct gctgggattc cgcacacacg
      961 cagactacgt cctggagatg aacatggcca agaccagtca gacagtagcc accttcctag
     1021 atgaactggc ccggaagctg aagccgctgg gtgagcagga gcgtgcggtg atcctggagc
     1081 tgaaggaggc agagtgcgcc aagcgcgggc tcccattcga tgggcgcatc cacgcatggg
     1141 acatgcggta ctacatgaac caggtggagg agacacgcta ccgcgtagac cagaacctgc
     1201 tgaaggagta cttccccatg caggtggtca cgcgtgggct gctggccatc taccaagagc
     1261 tgctggggct gaccttcacg ctggaggaag gtgccgccgc ctggcacgaa gacgtgcggc
     1321 tgtactctgt gcgtgacgcc gcctctggag aggagattgg caagttctac ctcgacctgt
     1381 accccaggga agggaagtat ggccacgcgg cctgcttcgg cctgcagcct ggctgcctac
     1441 ggcaggatgg cagccgacag ctggccatcg cagccatggt ggccaatttc accaagccca
     1501 cacctgacgt gccttccctg ctgcagcacg atgaggtgga gacctacttc cacgagttcg
     1561 ggcacgtcat gcaccagctc tgctcacagg cagagtttgc tatgttcagt gggacccacg
     1621 tggagcggga ctttgtggag gcaccgtcac agatgctgga gaactgggtg tgggaaaagg
     1681 agccactgat gcgcatgtcc cagcattacc gcacaggagg cgaggcccct gaggacctcc
     1741 tggagaagct catcaagtct cgccaggcca atgcaggtct cttcaacctt cggcagattg
     1801 tccttgccaa ggtagatcag gtcctgcaca cacagacaga tgtagaccca gctgaggaat
     1861 atgcccggct ctgccaggag atccttgggg tgccagccac accaggtacc aacatgccag
     1921 ccacttttgg ccacctcgct ggtggctacg acgctcagta ctatggctac ttgtggagtg
     1981 aggtgtactc catggacatg ttccacacac gcttcaagca ggagggtgtg cttagcccca
     2041 aggttggcat ggattaccgg accagcatcc tgaggccggg gggctctgag gatgccagca
     2101 ccatgctgaa gcagttcctg ggccgtgacc ccaagcaaga cgccttcctc ctgagcaagg
     2161 gtctgcaggt agagggctgc gagccacctg cgtgctgagc ggtccccagc tcgccctcct
     2221 gtgccttagc ctgccgggcg gcaaggtcag cagcccttgg gcgtacggat gagcctgggg
     2281 cagtttccat cctcgtcctc cccccagctc caggtccccg gcttcaggct gctgtgctct
     2341 ccttgcacta tcccctcccc aaactgatgt tggacattaa atttcacaca ggggaaaaaa
     2401 aaaaaaaa
//
