LOCUS       NM_017133               3221 bp    mRNA    linear   ROD 20-AUG-2024
DEFINITION  Rattus norvegicus thrombospondin 4 (Thbs4), mRNA.
ACCESSION   NM_017133 XM_001066338 XM_003753507 XM_342172
VERSION     NM_017133.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Zeng,H., Lan,B., Li,B., Xie,H., Zhao,E., Liu,X., Xue,X., Sun,J.,
            Su,L. and Zhang,Y.
  TITLE     The role and mechanism of thrombospondin-4 in pulmonary arterial
            hypertension associated with congenital heart disease
  JOURNAL   Respir Res 25 (1), 313 (2024)
   PUBMED   39154161
  REMARK    GeneRIF: The role and mechanism of thrombospondin-4 in pulmonary
            arterial hypertension associated with congenital heart disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Meng,X.M., Pang,Q.Y., Zhou,Z.F., Yuan,J.H., You,L., Feng,Q.P. and
            Zhu,B.M.
  TITLE     Histone methyltransferase MLL4 protects against pressure
            overload-induced heart failure via a THBS4-mediated protection in
            ER stress
  JOURNAL   Pharmacol Res 205, 107263 (2024)
   PUBMED   38876442
  REMARK    GeneRIF: Histone methyltransferase MLL4 protects against pressure
            overload-induced heart failure via a THBS4-mediated protection in
            ER stress.
REFERENCE   3  (bases 1 to 3221)
  AUTHORS   Guo,Y., Zhang,Z., Wu,H.E., Luo,Z.D., Hogan,Q.H. and Pan,B.
  TITLE     Increased thrombospondin-4 after nerve injury mediates disruption
            of intracellular calcium signaling in primary sensory neurons
  JOURNAL   Neuropharmacology 117, 292-304 (2017)
   PUBMED   28232180
  REMARK    GeneRIF: Results show that TSP4 regulates Ca2+ homeostasis in
            primary sensory neurons by affecting Ca2+ influx and buffering,
            including SERCA and PMCA, via PKC signaling after binding to
            Cavalpha2delta1. Elevated TSP4 expression may be a key factor that
            disrupts Ca2+ homeostasis after neuronal injury, and may serve as a
            valuable target for treatment of neuropathic pain.
REFERENCE   4  (bases 1 to 3221)
  AUTHORS   Yang,H.J., Ma,S.P., Ju,F., Zhang,Y.P., Li,Z.C., Zhang,B.B.,
            Lian,J.J., Wang,L., Cheng,B.F., Wang,M. and Feng,Z.W.
  TITLE     Thrombospondin-4 Promotes Neuronal Differentiation of NG2 Cells via
            the ERK/MAPK Pathway
  JOURNAL   J Mol Neurosci 60 (4), 517-524 (2016)
   PUBMED   27647309
  REMARK    GeneRIF: TSP4 promoted neuronal differentiation of NG2 cells by
            inhibiting ERK/MAPK signaling.
REFERENCE   5  (bases 1 to 3221)
  AUTHORS   Krawczyk,K.K., Ekman,M., Rippe,C., Grossi,M., Nilsson,B.O.,
            Albinsson,S., Uvelius,B. and Sward,K.
  TITLE     Assessing the contribution of thrombospondin-4 induction and
            ATF6alpha activation to endoplasmic reticulum expansion and
            phenotypic modulation in bladder outlet obstruction
  JOURNAL   Sci Rep 6, 32449 (2016)
   PUBMED   27581066
  REMARK    GeneRIF: The findings demonstrate reciprocal regulation of the
            unfolded protein response, including ATF6alpha activation and
            endoplasmic reticulum expansion, and reduced contractile
            differentiation in bladder outlet obstruction occurring
            independently of thrombospondin-4, which however is a sensitive
            indicator of obstruction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3221)
  AUTHORS   Stenina,O.I., Desai,S.Y., Krukovets,I., Kight,K., Janigro,D.,
            Topol,E.J. and Plow,E.F.
  TITLE     Thrombospondin-4 and its variants: expression and differential
            effects on endothelial cells
  JOURNAL   Circulation 108 (12), 1514-1519 (2003)
   PUBMED   12952849
REFERENCE   7  (bases 1 to 3221)
  AUTHORS   Narouz-Ott,L., Maurer,P., Nitsche,D.P., Smyth,N. and Paulsson,M.
  TITLE     Thrombospondin-4 binds specifically to both collagenous and
            non-collagenous extracellular matrix proteins via its C-terminal
            domains
  JOURNAL   J Biol Chem 275 (47), 37110-37117 (2000)
   PUBMED   10956668
REFERENCE   8  (bases 1 to 3221)
  AUTHORS   Arber,S. and Caroni,P.
  TITLE     Thrombospondin-4, an extracellular matrix protein expressed in the
            developing and adult nervous system promotes neurite outgrowth
  JOURNAL   J Cell Biol 131 (4), 1083-1094 (1995)
   PUBMED   7490284
REFERENCE   9  (bases 1 to 3221)
  AUTHORS   Lawler,J., McHenry,K., Duquette,M. and Derick,L.
  TITLE     Characterization of human thrombospondin-4
  JOURNAL   J Biol Chem 270 (6), 2809-2814 (1995)
   PUBMED   7852353
REFERENCE   10 (bases 1 to 3221)
  AUTHORS   Adams,J.C. and Lawler,J.
  TITLE     Cell-type specific adhesive interactions of skeletal myoblasts with
            thrombospondin-1
  JOURNAL   Mol Biol Cell 5 (4), 423-437 (1994)
   PUBMED   7519904
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before Sep 1, 2012 this sequence version replaced
            XM_001066338.3, XM_003753507.1, XM_342172.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643296.5055.1,
                                           SRR26643299.8155.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676784 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               JAXUCZ010000002.1  25760084-25760345   c
            263-466             JAXUCZ010000002.1  25756105-25756308   c
            467-714             JAXUCZ010000002.1  25742484-25742731   c
            715-823             JAXUCZ010000002.1  25741726-25741834   c
            824-906             JAXUCZ010000002.1  25741118-25741200   c
            907-958             JAXUCZ010000002.1  25740810-25740861   c
            959-1161            JAXUCZ010000002.1  25740396-25740598   c
            1162-1299           JAXUCZ010000002.1  25738955-25739092   c
            1300-1368           JAXUCZ010000002.1  25736078-25736146   c
            1369-1521           JAXUCZ010000002.1  25733269-25733421   c
            1522-1626           JAXUCZ010000002.1  25729564-25729668   c
            1627-1734           JAXUCZ010000002.1  25729217-25729324   c
            1735-1894           JAXUCZ010000002.1  25728803-25728962   c
            1895-2013           JAXUCZ010000002.1  25727812-25727930   c
            2014-2066           JAXUCZ010000002.1  25726892-25726944   c
            2067-2260           JAXUCZ010000002.1  25724250-25724443   c
            2261-2439           JAXUCZ010000002.1  25722425-25722603   c
            2440-2488           JAXUCZ010000002.1  25721338-25721386   c
            2489-2685           JAXUCZ010000002.1  25721057-25721253   c
            2686-2858           JAXUCZ010000002.1  25720302-25720474   c
            2859-2998           JAXUCZ010000002.1  25718969-25719108   c
            2999-3221           JAXUCZ010000002.1  25718219-25718441   c
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..3221
                     /gene="Thbs4"
                     /note="thrombospondin 4"
                     /db_xref="GeneID:29220"
                     /db_xref="RGD:62046"
     exon            1..262
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..3060
                     /gene="Thbs4"
                     /codon_start=1
                     /product="thrombospondin-4 precursor"
                     /protein_id="NP_058829.1"
                     /db_xref="GeneID:29220"
                     /db_xref="RGD:62046"
                     /translation="MLAPRAAAFLLLHLVLQPWQRAGAQATPQVFDLLPSSSQRLNPA
                     ALQPVLTDPTLHELYVISTFKLQSKSSATIFGLYSSSDNSKYFEFTVMGRLNKAILRY
                     LKDDGKIHLVVFNNLQLADGRRHRILLRLSNLQRGAGSVELYLDCVQVDSVNNLPRAF
                     SGLTQNPQAIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEPLAATGTGD
                     FNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLSFQSPTPNTLVP
                     IAPPAPPTRPTRRCDSSPCFRGVRCTDTRDGFQCGPCPDGYTGNGITCSDVDECKYHP
                     CYPGVRCTNLAPGFRCDACPVGFTGPMVQGVGINFAKTNKQVCTDVDECRNGACVLNS
                     ICINTLGSYRCGPCKPGYTGDQTRGCRTERSCRNPEQNPCSVHAQCIEERQGDVTCVC
                     GVGWAGDGYVCGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACD
                     EDADGDGILNEQDNCVLTHNVDQRNTDKDIFGDACDNCRGVLNNDQKDTDGDGKGDAC
                     DDDMDGDGIKNILDNCPRVPNRDQQDRDGDGVGDACDSCPDVSNPNQSDVDNDLVGDS
                     CDTNQDSDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDN
                     CRLVPNPAQEDSNNDGVGDICEADFDQDKVIDRIDVCPENAEITLTDFRAYQTVVLDP
                     EGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGF
                     IFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHT
                     GDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDT
                     TMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQIQTFDRLDN"
     exon            263..466
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            467..714
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            715..823
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            824..906
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            907..958
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1161
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1299
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1368
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1521
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1626
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1734
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1894
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2013
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2066
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2260
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2439
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2488
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2685
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2858
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2998
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3221
                     /gene="Thbs4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggctgcgg ccgcggaacc cagcagccag cgcccctccg acggctgcgc gctgccctgc
       61 tcgccacctg ctccgctcac tgcccgaggc cagctgacag cgccctaccc cggcagagcc
      121 acacagaggc tgtcgctgtc gcctgtacag ccttcgggga gcagaagagt gaccatgcta
      181 gccccacgcg cggccgcctt cctcctactg cacctggtcc tgcagccgtg gcagcgggcg
      241 ggcgcccagg ccacacccca ggtctttgac cttctgccat cctccagcca gaggctgaac
      301 ccggccgccc tgcagccagt cctgacagac cccaccctgc acgagctcta tgtcatctcc
      361 accttcaagc tgcagagtaa gagctcagcc accatctttg gcctctactc ctcgagtgac
      421 aacagcaaat actttgaatt cactgtgatg ggacgcctga acaaagccat cctccgttac
      481 ctaaaggatg atgggaagat tcatctggtg gttttcaaca acctgcagct ggcggatggg
      541 aggcgccacc ggattctcct gagactcagc aatttgcaga gaggggccgg ctccgtagaa
      601 ctctacttgg actgtgtcca ggttgattca gtgaacaacc tccccagagc cttctctggt
      661 ctcacccaga acccacaggc cattgaattg agaacattcc agaggaagcc ccaggacttc
      721 ttggaagagc tgaagctggt ggtgaggggc tctctgttcc aggtggccag cctacaagac
      781 tgtttcctgc agcagagtga gccgctggct gccacaggca caggagactt taatcgacag
      841 tttttgggtc aaatgacaca attaaaccag ctccttggag aggtgaaaga tcttctgagg
      901 cagcaggtca aggaaacatc atttttgcga aacactattg ctgagtgcca ggcttgtggc
      961 cctctcagct tccagtcccc aaccccaaac acgctggtgc ccatagctcc tccagcaccc
     1021 ccaacccgac caacacgtcg ctgtgactcc agcccttgtt tccgaggtgt ccgatgcacg
     1081 gacaccaggg acggcttcca atgtggaccc tgcccagatg gctacacggg gaacgggatc
     1141 acctgttctg acgtggatga gtgtaaatac catccctgct accctggtgt gcgctgcaca
     1201 aatttggctc cgggtttcag atgtgatgcc tgtccagtag gcttcacggg gcccatggtg
     1261 cagggcgtgg ggatcaactt tgccaaaaca aacaagcagg tgtgcactga tgttgacgag
     1321 tgtcggaacg gagcgtgtgt tctgaactcc atctgcatta acactctggg ctcttaccgc
     1381 tgtgggcctt gcaagccagg gtacactggt gaccagacga ggggatgcag gaccgaaagg
     1441 agctgccgga acccagagca gaacccgtgc agtgtgcatg cgcagtgcat tgaggaacgg
     1501 caaggggatg tgacgtgtgt gtgcggtgtt ggttgggccg gcgacggcta tgtctgtgga
     1561 aaggatgtgg acatcgacag ttaccccgat gaagaactgc cgtgctcagc caggaactgc
     1621 aagaaggata actgcaagta tgtgccaaac tccggccaag aagacgcaga cagagatggc
     1681 attggagacg cctgtgatga ggatgcggat ggagacggga tcttgaatga gcaggacaac
     1741 tgtgtcctga ctcacaatgt ggaccagagg aacactgata aagatatctt tggggatgcc
     1801 tgtgacaact gccggggcgt cctgaataat gaccagaagg acactgacgg ggatgggaaa
     1861 ggagacgcct gtgatgacga catggatgga gacggaataa aaaacatttt ggacaactgt
     1921 ccccgagttc ccaaccgcga ccaacaggat cgggatggcg atggtgtcgg agacgcctgt
     1981 gacagctgtc ctgatgtcag taaccctaac cagtcggatg tggataacga tctggttggg
     2041 gactcctgtg acaccaacca agacagtgat ggggatggcc accaggacag cacagacaac
     2101 tgccccaccg tcattaacag cgcccagctg gacaccgaca aggacgggat tggcgatgaa
     2161 tgcgatgacg atgacgataa tgacggcatc ccagacctgg ttccccctgg cccagacaac
     2221 tgcaggcttg ttcccaaccc agcccaggaa gatagcaaca atgatggagt aggagatatc
     2281 tgtgaagctg acttcgacca ggacaaggtc attgatcgca ttgatgtgtg tcctgagaat
     2341 gcggagatca ccctgactga cttcagggcc tatcagactg tggtcctgga ccctgaaggg
     2401 gatgcccaga tcgaccccaa ctgggtggtc ctgaaccagg gcatggagat tgtgcagacc
     2461 atgaacagtg accctggcct agcagtcggg tacacggcat ttaatggagt tgactttgaa
     2521 gggaccttcc acgtaaacac ccagacagat gacgactatg ccggctttat ttttggttac
     2581 caagacagtt ccagcttcta tgtggtcatg tggaaacaga cagagcagac atactggcag
     2641 gctacgccat tccgtgcagt agcagaacct ggtattcagc tcaaggccgt gaagtctaaa
     2701 actggcccag gggaacatct ccgaaactcc ctgtggcaca caggggacac cagcgaccag
     2761 gtcaggctgc tgtggaagga ctccaggaat gtgggctgga aggacaaggt gtcctaccgc
     2821 tggttcctgc agcataggcc ccaggtgggc tacatcaggg tgcggtttta tgaaggctca
     2881 gagttggtgg ctgactccgg ggtcactata gacaccacca tgcgcggagg ccgactgggc
     2941 gtgttctgct tctctcagga aaacatcatc tggtccaacc tcaagtaccg ctgtaacgac
     3001 accataccag aggacttcca ggagttccag atccagacct ttgaccgcct ggataattaa
     3061 accaagaagc aatctcacaa ctgccttctc aaacgcccaa accaacatct tttgtttaaa
     3121 acttcctata attctcttta tattaccgtg gcttttcttt taccaaccca aatacttcaa
     3181 aactttttat acgaaataaa gaagagacta tttctaataa c
//
