LOCUS       NM_030988               8461 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus thyroglobulin (Tg), transcript variant 1, mRNA.
ACCESSION   NM_030988
VERSION     NM_030988.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Siffo,S., Gomes Pio,M., Martinez,E.B., Lachlan,K., Walker,J.,
            Weill,J., Gonzalez-Sarmiento,R., Rivolta,C.M. and Targovnik,H.M.
  TITLE     The p.Pro2232Leu variant in the ChEL domain of thyroglobulin gene
            causes intracellular transport disorder and congenital
            hypothyroidism
  JOURNAL   Endocrine 80 (1), 47-53 (2023)
   PUBMED   36547798
  REMARK    GeneRIF: The p.Pro2232Leu variant in the ChEL domain of
            thyroglobulin gene causes intracellular transport disorder and
            congenital hypothyroidism.
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Zhang,X., Malik,B., Young,C., Zhang,H., Larkin,D., Liao,X.H.,
            Refetoff,S., Liu,M. and Arvan,P.
  TITLE     Maintaining the thyroid gland in mutant thyroglobulin-induced
            hypothyroidism requires thyroid cell proliferation that must
            continue in adulthood
  JOURNAL   J Biol Chem 298 (7), 102066 (2022)
   PUBMED   35618019
  REMARK    GeneRIF: Maintaining the thyroid gland in mutant
            thyroglobulin-induced hypothyroidism requires thyroid cell
            proliferation that must continue in adulthood.
REFERENCE   3  (bases 1 to 8461)
  AUTHORS   Citterio,C.E., Siffo,S., Moya,C.M., Pio,M.G., Molina,M.F.,
            Scheps,K.G., Rey,O.A., Arvan,P., Rivolta,C.M. and Targovnik,H.M.
  TITLE     p.L571P in the linker domain of rat thyroglobulin causes
            intracellular retention
  JOURNAL   Mol Cell Endocrinol 505, 110719 (2020)
   PUBMED   31972331
  REMARK    GeneRIF: p.L571P in the linker domain of rat thyroglobulin causes
            intracellular retention.
REFERENCE   4  (bases 1 to 8461)
  AUTHORS   Coscia,F., Taler-Vercic,A., Chang,V.T., Sinn,L., O'Reilly,F.J.,
            Izore,T., Renko,M., Berger,I., Rappsilber,J., Turk,D. and Lowe,J.
  TITLE     The structure of human thyroglobulin
  JOURNAL   Nature 578 (7796), 627-630 (2020)
   PUBMED   32025030
REFERENCE   5  (bases 1 to 8461)
  AUTHORS   Chen,Y., Wang,N., Chen,Y., Li,Q., Han,B., Chen,C., Zhai,H. and
            Lu,Y.
  TITLE     The association of non-alcoholic fatty liver disease with thyroid
            peroxidase and thyroglobulin antibody: A new insight from
            SPECT-China study
  JOURNAL   Autoimmunity 51 (5), 238-244 (2018)
   PUBMED   30016121
REFERENCE   6  (bases 1 to 8461)
  AUTHORS   Sugisaki,T., Beamer,W.G. and Noguchi,T.
  TITLE     Microcephalic cerebrum with hypomyelination in the congenital
            goiter mouse (cog)
  JOURNAL   Neurochem Res 17 (10), 1037-1040 (1992)
   PUBMED   1508304
REFERENCE   7  (bases 1 to 8461)
  AUTHORS   Ieiri,T., Cochaux,P., Targovnik,H.M., Suzuki,M., Shimoda,S.,
            Perret,J. and Vassart,G.
  TITLE     A 3' splice site mutation in the thyroglobulin gene responsible for
            congenital goiter with hypothyroidism
  JOURNAL   J Clin Invest 88 (6), 1901-1905 (1991)
   PUBMED   1752952
REFERENCE   8  (bases 1 to 8461)
  AUTHORS   Graves,P.N. and Davies,T.F.
  TITLE     A second thyroglobulin messenger RNA species (rTg-2) in rat
            thyrocytes
  JOURNAL   Mol Endocrinol 4 (1), 155-161 (1990)
   PUBMED   2325666
REFERENCE   9  (bases 1 to 8461)
  AUTHORS   Uller,R.P. and Van Herle,A.J.
  TITLE     Effect of therapy on serum thyroglobulin levels in patients with
            Graves' disease
  JOURNAL   J Clin Endocrinol Metab 46 (5), 747-755 (1978)
   PUBMED   95586
REFERENCE   10 (bases 1 to 8461)
  AUTHORS   Berg,G. and Ekholm,R.
  TITLE     Electron microscopy of low iodinated thyroglobulin molecules
  JOURNAL   Biochim Biophys Acta 386 (2), 422-431 (1975)
   PUBMED   1138879
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Jul 31, 2012 this sequence version replaced NM_030988.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB035201.1, AB679745.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               JAXUCZ010000007.1  100307349-100307458
            111-219             JAXUCZ010000007.1  100307900-100308008
            220-317             JAXUCZ010000007.1  100308991-100309088
            318-521             JAXUCZ010000007.1  100310013-100310216
            522-681             JAXUCZ010000007.1  100310540-100310699
            682-788             JAXUCZ010000007.1  100318165-100318271
            789-932             JAXUCZ010000007.1  100318941-100319084
            933-1118            JAXUCZ010000007.1  100319279-100319464
            1119-2216           JAXUCZ010000007.1  100322711-100323808
            2217-2804           JAXUCZ010000007.1  100324468-100325055
            2805-3044           JAXUCZ010000007.1  100326088-100326327
            3045-3182           JAXUCZ010000007.1  100329572-100329709
            3183-3260           JAXUCZ010000007.1  100330056-100330133
            3261-3373           JAXUCZ010000007.1  100330674-100330786
            3374-3476           JAXUCZ010000007.1  100333122-100333224
            3477-3677           JAXUCZ010000007.1  100334083-100334283
            3678-3887           JAXUCZ010000007.1  100335459-100335668
            3888-4042           JAXUCZ010000007.1  100336833-100336987
            4043-4199           JAXUCZ010000007.1  100337206-100337362
            4200-4415           JAXUCZ010000007.1  100339189-100339404
            4416-4565           JAXUCZ010000007.1  100346220-100346369
            4566-4736           JAXUCZ010000007.1  100349072-100349242
            4737-4853           JAXUCZ010000007.1  100353603-100353719
            4854-4969           JAXUCZ010000007.1  100355632-100355747
            4970-5078           JAXUCZ010000007.1  100358833-100358941
            5079-5270           JAXUCZ010000007.1  100364384-100364575
            5271-5438           JAXUCZ010000007.1  100369327-100369494
            5439-5504           JAXUCZ010000007.1  100374094-100374159
            5505-5585           JAXUCZ010000007.1  100374588-100374668
            5586-5723           JAXUCZ010000007.1  100375886-100376023
            5724-5900           JAXUCZ010000007.1  100377281-100377457
            5901-6012           JAXUCZ010000007.1  100378876-100378987
            6013-6092           JAXUCZ010000007.1  100380537-100380616
            6093-6236           JAXUCZ010000007.1  100381317-100381460
            6237-6302           JAXUCZ010000007.1  100388220-100388285
            6303-6437           JAXUCZ010000007.1  100396962-100397096
            6438-6602           JAXUCZ010000007.1  100398256-100398420
            6603-6825           JAXUCZ010000007.1  100402265-100402487
            6826-6919           JAXUCZ010000007.1  100403940-100404033
            6920-7079           JAXUCZ010000007.1  100406334-100406493
            7080-7282           JAXUCZ010000007.1  100411324-100411526
            7283-7447           JAXUCZ010000007.1  100469274-100469438
            7448-7615           JAXUCZ010000007.1  100470490-100470657
            7616-7797           JAXUCZ010000007.1  100480122-100480303
            7798-7905           JAXUCZ010000007.1  100481261-100481368
            7906-8040           JAXUCZ010000007.1  100488766-100488900
            8041-8228           JAXUCZ010000007.1  100491144-100491331
            8229-8461           JAXUCZ010000007.1  100492014-100492246
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q33"
     gene            1..8461
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /note="thyroglobulin"
                     /db_xref="GeneID:24826"
                     /db_xref="RGD:3848"
     exon            1..110
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..8347
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="thyroglobulin isoform 1 precursor"
                     /protein_id="NP_112250.2"
                     /db_xref="GeneID:24826"
                     /db_xref="RGD:3848"
                     /translation="MMTLVLWVSTLLSSVCLVAANIFEYQVDAQPLRPCELQREKAFL
                     KQDEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEVPGSRQLGRPTACLSFCQLHKQ
                     RILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYGTRQQGRPTR
                     CPRSCEIRSRRLLHGVGDKSPPQCDADGEFMPVQCKFVNTTDMMIFDLIHNYNRFPDA
                     FVTFSAFRNRFPEVSGYCYCADSQGRELAETGLELLLDEIYDTIFAGLDQASTFTQST
                     MYRILQRRFLAIQLVISGRFRCPTKCEVEQFTATSFGHPYIPSCHRDGHYQTVQCQME
                     RMCWCVDAQGIEIPGTRQQGQPLFCAKDQSCASERQQALSRLYFETPGYFSPQDLLSS
                     EDRLVPVSGARLDISCPPRIKELFVDSGLLRSIAVERYQQLSESRSLLREAIRAIFPS
                     RELAGLALQFTTNPKRLQQNLFGGTFLVNAAQLNLSGALGTRSTFNFSQFFQQFGLPG
                     FLVRDRATDLAKLLPVSLDSSPTPVPLRVPEKRVAMNKSVVGTFGFKVNLQENQDALK
                     FLVSLMELPEFLVFLQRAVSVPEDRARDLGDVMEMVFSAQACKQTSGRFFVPSCTAEG
                     SYEDIQCYAGECWCVNSQGKEVEGSRVSGGHPRCPTKCEKQRAQMQNLAGAQPAGSSF
                     FVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPGTQSTIGEPKLCPSVCQLQAEQAFLG
                     VVGVLLSNSSMVPPISSVYIPQCSTSGQWMPVQCDGPHEQVFEWYERWNTQNSDGQEL
                     TTATLLMKLMSYREVASTNFSLFLQSLYDAGQQSIFPVLAQYPSLQDVPQVVLEGATI
                     QPGENIFLDPYIFWQILNGQLSQYPGPYSDFSMPLEHFNLRSCWCVDEAGQELDGTRT
                     RAGEIPACPGPCEEVKFRVLKFIKETEEIVSASNASSFPLGESFLVAKGIQLTSEELG
                     LPPLYPSREAFSEKFLRGSEYAIRLAAQSTLTFYQKLRASLGESNGTASLLWSGPYMP
                     QCNTIGGWEPVQCHPGTGQCWCVDGWGELIPGSLMARSSQMPQCPTSCELSRANGLIS
                     AWKQAGHQRNPGPGDLFTPVCLQTGEYVRQQTSGTGAWCVDPSSGEGVPTNTNSSAQC
                     PGLCDALKSRVLSRKVGLGYTPVCEALDGGFSPVQCDLAQGSCWCVLASGEEVPGTRV
                     VGTQPACESPQCPLPFSGSDVTDGVVFCETASSSGVTTVQQCQLFCRQGLWNVFSPGP
                     LICNLESQRWVTLPLPRACQRPQLWQTMQTQAHFQLLLPPGKMCSIDYSGLLQAFQVF
                     ILDELITRGFCQIQVKTFGTLVSRTVCDNSSIQVGCLTAERLGVNATWKLQLEDISVG
                     SLPNLHSIERALMGQDLLGRFANLIQSGKFQLHLDSKTFSADTILYFLNGDRFVTSPM
                     TQLGCLEGFYRVSTTSQDPLGCVKCPEGSFSQDGKCTPCPAGTYQGQAGSSACIPCPR
                     GRTTTITGAFSKTHCVTDCQRDEAGLQCDQNGQYQANQKDMDSGEVFCVDSEGQRLQW
                     LQTEAGLSESQCLMMRKFEKAPESKVIFDASSPVIVKSRVPSANSPLVQCLADCADDE
                     ACSFVTVSSMSSEVSCDLYSWTRDNFACVTSDQEEDAVDSLKETSFGSLRCQVKVRNS
                     GKDSLAVYVKKGHEFTASGQKSFEPTGFQNVLSGLYSSVVFSALGTNLTDTHLFCLLA
                     CDQDSCCDGFIVTQVKEGPTICGLLSAPDILVCHINDWRDASDTQANGTCAGVTYDQG
                     SRQMTMSLGGQEFLQGLTLLEGTQDSFISFQQVYLWKDSDIGSRPESMGCGRGMVPKS
                     EAPEGADMATELFSPVDITQVIVNTSHSLPSQQYWLSTHLFSAEQANLWCLSRCAQEP
                     VFCQLADIMESSSLYFTCSLYPEAQVCDNDVESNAKNCSQILPRQPTALFQRKVVLND
                     RVKNFYTRLPFQKLSGISIRDRIPMSEKLISNGFFECERLCDRDPCCTGFGFLNVSQM
                     QGGEMTCLTLNSMGIQTCSEENGATWRILDCGSEDTEVHTYPFGWYQKPAVWSDAPSF
                     CPSAALQSLTEEKVALDSWQTLALSSVIIDPSIKHFDVAHISISATRNFSLAQDFCLQ
                     ECSRHQDCLVTTLQIQQGVVRCVFYPDIQSCEHSLRSKTCWLLLHEEAAYIYRKSGAP
                     LHQSDGISTPSVHIDSFGQLQGGSQVVKVGTAWKQVYQFLGVPYAAPPLAENRFQAPE
                     VLNWTGSWDATKLRSSCWQPGTRTPTPPQISEDCLYLNVFVPENLVSNASVLVFFHNT
                     VEMEGSGGQLNIDGSILAAVGNLIVVTANYRLGVFGFLSSGSDEVAGNWGLLDQVAAL
                     TWVQTHIGAFGGDPQRVTLAADRGGADVASIHLLITRPTRLQLFRKALLMGGSALSPA
                     AIISPDRAQQQAAALAKEVGCPNSSVQEVVSCFRQKPANILNEAQTKLLAVSGPFHYW
                     GPVVDGQYLRELPSRRLKRPLPVKVDLLIGGSQDDGLINRAKAVKQFEESQGRTNSKT
                     AFYQALQNSLGGEDSDARILAAAIWYYSLEHSTDDYASFSRALENATRDYFIICPIVN
                     MASLWARRTRGNVFMYHVPESYGHGSLELLADVQYAFGLPFYSAYQGYFSMEEQSLSL
                     KVMQYFSNFIRSGNPNYPHEFSQKAAEFATPWPDFVPGAGGESYKELSAQLPNRQGLK
                     KADCSFWSKYIQTLKDADGAKDGQLTKSGEEDLEVGPGSEEDFSGSLEPVPKSYSK"
     exon            111..219
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            220..317
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            318..521
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            522..681
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            682..788
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            789..932
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1118
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..2216
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2804
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..3044
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3182
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3260
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3261..3373
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3374..3476
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3677
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3678..3887
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..4042
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4043..4199
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4200..4415
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4416..4565
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4566..4736
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4737..4853
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4854..4969
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            4970..5078
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5079..5270
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5271..5438
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5439..5504
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5505..5585
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5586..5723
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5724..5900
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            5901..6012
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6013..6092
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6093..6236
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6237..6302
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6303..6437
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6438..6602
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6603..6825
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6826..6919
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            6920..7079
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7080..7282
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7283..7447
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7448..7615
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7616..7797
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7798..7905
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            7906..8040
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            8041..8228
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
     exon            8229..8461
                     /gene="Tg"
                     /gene_synonym="Tgn"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggacactgg tcctacccca tcatttgagt aggggacagg atgatgacct tggtcttgtg
       61 ggtctcgact ttgttgagct cagtctgcct ggtagcagcc aacatctttg agtaccaagt
      121 ggatgcacag ccactccgcc cctgtgagct gcaaagggag aaggcctttc tgaagcagga
      181 tgaatatgtt ccacagtgct ctgaagatgg aagtttccag acagttcagt gccaaaatga
      241 tggccaatct tgctggtgtg tggattctga tggcacggaa gtacctggca gcaggcagct
      301 gggaaggcca acagcatgtc tgtccttctg ccagctacac aagcaacgaa tcctgctgag
      361 cagctacatt aacagcacag atgctctcta ccttcctcag tgccaggatt cagggaacta
      421 tgcaccagtg cagtgcgacc tgcagcaagt gcaatgctgg tgtgtagaca cagaggggat
      481 ggaggtgtat ggcacccgcc agcaggggag gccaacacgg tgtccaagga gctgtgagat
      541 aagaagccgc cgtctcctcc atggagtagg agacaaatcg ccaccccagt gcgatgcaga
      601 tggggagttt atgcctgtcc agtgcaagtt tgtcaacacc acagacatga tgatttttga
      661 tctgattcac aactacaaca ggtttccaga tgcatttgtg accttcagtg ccttccggaa
      721 caggttccct gaagtgtctg ggtactgtta ctgtgctgac agccagggcc gagagctggc
      781 tgagacagga ctggagttgt tactggatga aatttatgac accatttttg ctggcttgga
      841 ccaggcctcc accttcactc aaagcaccat gtaccggata ttgcagagac gattcctggc
      901 cattcaatta gtcatctctg gcagattccg atgtcccacc aagtgtgaag tggaacagtt
      961 tactgccacc agctttggtc atccatatat cccaagctgc cacagagatg ggcactacca
     1021 aacagtgcag tgccagatgg aaaggatgtg ctggtgtgtg gatgctcagg gaatagagat
     1081 ccctggcact cggcagcaag ggcagccatt gttttgcgct aaagatcaat cgtgtgcctc
     1141 agaaaggcag caggctttgt ctaggctcta ctttgaaacc ccaggctact tcagtcctca
     1201 ggacctgttg tcttctgagg acaggctggt ccctgtgagt ggtgccagat tggatatatc
     1261 ctgcccaccc agaatcaagg aactctttgt cgactctgga ctcctacgtt ctattgctgt
     1321 ggagcgctac caacaactct ctgaatccag aagtctactt agagaagcca tcagagcaat
     1381 tttcccctcc cgagagctgg cagggcttgc ccttcagttc accaccaacc ccaagcgact
     1441 ccagcaaaac ctctttggag ggacattttt ggtgaatgct gcccagctaa acttgtcagg
     1501 agcccttggt acaagaagca catttaactt cagtcagttt ttccagcagt ttggactccc
     1561 aggcttcctg gttagagaca gagctactga tctagccaaa ctactccctg tgagtttgga
     1621 ctccagtcct accccagtac ctctccgtgt gcctgagaag agagttgcta tgaataagtc
     1681 agttgtgggt acctttggct tcaaagtcaa cctacaagag aaccaagatg ccttaaaatt
     1741 ccttgtttct ctcatggaac ttccagaatt tcttgtcttc ttgcagcgtg ctgtttctgt
     1801 acctgaagat agagcaagag atttaggaga tgtgatggaa atggtgttca gtgctcaggc
     1861 ctgtaaacag acatctggga ggttctttgt cccatcatgc actgctgaag gaagctatga
     1921 agatatccag tgctatgctg gagaatgctg gtgtgtgaat tcccaaggca aagaagttga
     1981 aggctcaaga gtcagtgggg gacacccaag atgtcccacc aagtgtgaga agcaaagagc
     2041 tcagatgcaa aaccttgcag gcgctcagcc tgctggctcc agtttttttg ttcctacgtg
     2101 tactagtgag ggatattttc tgcccgtcca gtgcttcaac tcagaatgtt actgtgttga
     2161 tgctgaaggc caggttattc ctggaactca aagtacaatt ggtgaaccca agctgtgtcc
     2221 tagtgtctgc cagttacagg ctgaacaagc tttccttggg gtagtagggg ttctgctctc
     2281 caactccagt atggtgcctc ctatctccag tgtctacatt ccccaatgca gtaccagtgg
     2341 ccagtggatg cctgtgcagt gtgatggacc ccatgagcag gtctttgagt ggtatgaacg
     2401 atggaacact cagaacagtg atggccagga actgactaca gccacactgc tgatgaagct
     2461 catgagctac agagaagtag cttccacaaa cttcagcctt tttcttcaaa gtctctatga
     2521 tgctggccag caaagcatct tcccagtgct agcacagtac ccatcccttc aggatgttcc
     2581 acaggtagtg ttggagggag ccacaattca gcctggagag aacattttcc tggatccata
     2641 catcttttgg cagatcctaa atggccagct cagccaatac ccaggaccct actcagactt
     2701 tagcatgcct ttggaacact tcaacctcag gagctgctgg tgtgtggatg aggctggtca
     2761 ggaattggat ggaactcgga ctagggctgg cgagatacca gcatgccctg gcccctgtga
     2821 ggaagtgaaa tttcgtgtcc tgaaatttat caaggaaaca gaagagatcg tctctgcttc
     2881 caatgcttct tcatttccac ttggagagag tttcttagta gccaagggaa tccagctgac
     2941 cagtgaggag cttggccttc ctccactcta cccatctcgt gaggctttct ctgagaagtt
     3001 tctacgtggg agtgaatatg ccattcgttt ggctgctcag tccaccttga ccttctatca
     3061 gaaactccgt gcttccctgg gagagtccaa tggaacagcc agtcttctat ggtcaggacc
     3121 ctatatgcca caatgcaata ctattggagg ctgggagcct gtgcagtgcc atcctgggac
     3181 aggacagtgt tggtgtgtgg atgggtgggg agagctcatc ccaggctcac tgatggcacg
     3241 ctcctcccag atgccccagt gtcccacaag ctgtgagcta tcccgagcca atgggttgat
     3301 ttctgcatgg aaacaggctg gacaccaaag aaacccaggt ccaggagatc tgtttacacc
     3361 agtctgccta cagacaggag agtatgtcag acaacagaca tcaggaacag gagcttggtg
     3421 tgtggatcca tcttcaggag aaggggtgcc aacaaacaca aacagcagtg ctcagtgtcc
     3481 tggcctctgt gatgccctca agagtagggt cctgtccagg aaagtcggtc ttggctacac
     3541 tccagtctgt gaggcattgg atgggggctt ttccccagtg cagtgtgact tggcccaggg
     3601 cagttgctgg tgtgtcctgg caagtggaga ggaagtgcct gggactcgtg tggtaggcac
     3661 ccagcctgcc tgtgaaagcc cacagtgtcc actgccattc tctgggtcag atgtgactga
     3721 tggagtggtc ttctgtgaga cagcctctag ctcaggagtg accactgttc aacagtgcca
     3781 gctgttctgc cgccagggcc tctggaacgt cttttcacca gggccactga tttgtaacct
     3841 ggagagtcag cgctgggtga cactgccact acccagagcc tgtcagaggc ctcagctgtg
     3901 gcagaccatg caaactcaag cacacttcca gctcctgctc ccaccaggca agatgtgcag
     3961 tattgactac tctggcttgc tgcaggcctt ccaggtcttc atactggatg agctgataac
     4021 ccgtggcttc tgccagatcc aggtaaagac atttgggacc ctggtttcca ggactgtctg
     4081 tgacaactcc tcaatacagg tggggtgtct gactgcagag cgtttaggag taaatgctac
     4141 atggaagtta cagcttgagg acatctcagt gggctcactt ccaaatttgc acagcattga
     4201 aagggcatta atgggccagg atctccttgg gcgctttgca aatctgatac aaagtggcaa
     4261 gttccagctt catctggact ctaagacatt ctcagctgat accatcttgt actttctcaa
     4321 tggggacaga tttgtcacct ctcccatgac tcagcttgga tgcttggaag gattttacag
     4381 agtttcaacc accagtcagg accctctggg ctgtgtgaaa tgccctgaag gaagcttctc
     4441 tcaggatgga aaatgcactc cctgtcctgc tggcacctac caagggcagg caggcagctc
     4501 agcttgcatc ccatgcccaa ggggcagaac aaccaccatc actggagctt tcagcaagac
     4561 ccattgtgtc actgactgtc agagggatga ggccggccta cagtgtgacc agaatggtca
     4621 gtaccaagcc aaccagaagg acatggacag tggagaagtt ttctgcgtgg atagcgaggg
     4681 gcagagacta cagtggttac agacagaggc tggactctca gaatcccagt gtctgatgat
     4741 gcggaaattt gagaaagctc cagaatccaa ggttatcttt gatgccagtt ctcctgtgat
     4801 agtcaagtcc agagtcccca gtgccaactc cccactggtg cagtgcttag cagattgtgc
     4861 agatgatgaa gcctgcagct tcgtcacagt gtcctcaatg tcatcagagg tttcatgtga
     4921 tctttattct tggacaagag acaactttgc ctgtgtgact tctgatcagg aagaagatgc
     4981 tgtggacagc ttgaaggaaa ccagctttgg aagccttagg tgccaggtga aagtgaggaa
     5041 tagtggcaag gattctctgg ctgtgtatgt gaagaaaggc catgaattta ctgcatctgg
     5101 tcagaagagc ttcgaaccaa caggttttca aaatgtgctc tcggggttgt atagctctgt
     5161 ggtgttttca gccttgggaa ccaatctgac agatacacac ctcttctgtc tgcttgcctg
     5221 tgaccaggat tcctgctgcg atggcttcat cgtcacacag gtcaaagaag ggcctaccat
     5281 ctgtggactt ctcagtgctc ctgacattct ggtttgccac atcaatgact ggagggatgc
     5341 ctctgatact caggccaatg gtacatgtgc tggggtgacg tatgaccaag ggagccgcca
     5401 gatgaccatg agtctgggag gccaagaatt cttgcagggt ctgacgctct tggagggaac
     5461 tcaagacagc tttatcagct tccagcaggt ctacctctgg aaagattctg acatagggtc
     5521 tcggcctgaa tctatgggat gtggaagggg aatggtgccc aagtcagaag ctccagaagg
     5581 agcagatatg gcaacagaac ttttctcccc tgtggacatt acacaggtca ttgtcaatac
     5641 aagccactca ttgcccagcc agcagtactg gctctccaca cacctgtttt cagcagagca
     5701 agcaaacctg tggtgccttt ctcggtgtgc ccaggagcct gttttctgtc aacttgcaga
     5761 cataatggaa agctcatctt tgtatttcac atgctcccta tacccagaag ctcaggtctg
     5821 tgacaatgat gtggaatcta atgccaagaa ctgcagtcag attctgcccc gccaaccaac
     5881 agccctcttc cagaggaaag ttgtgctgaa tgaccgagtg aagaactttt atactcgcct
     5941 gccattccaa aagctctcag ggatttccat cagagacaga atccccatgt ctgaaaaact
     6001 catttccaat gggttctttg agtgtgaacg cctctgtgac agagacccat gctgcactgg
     6061 ctttggtttt ctaaatgtct cccagatgca aggaggagag atgacctgct tgactctgaa
     6121 cagcatgggg attcaaacat gcagtgagga gaatggagca acctggcgta ttttggactg
     6181 tggctctgaa gacactgaag tccacaccta tccctttgga tggtaccaaa agcctgctgt
     6241 gtggagtgat gctcccagct tctgcccatc tgctgccctg caatcactca ctgaggagaa
     6301 ggttgctttg gactcctggc agactctggc cctatcttca gtgattatag atccatccat
     6361 caagcacttt gatgttgccc acatcagcat ttcagccacc aggaacttct ccctggccca
     6421 agacttctgc ttacaggaat gttcccgtca tcaggactgc cttgtcacta ccctgcagat
     6481 ccagcaaggg gttgtgaggt gtgtgttcta ccctgatata cagagctgcg aacacagtct
     6541 acggagcaag acctgctggc ttctgctcca tgaagaggct gcctacatct accggaaatc
     6601 tggagcccct ttgcaccaat ctgatggaat atccacacct tctgtacaca ttgattcctt
     6661 tggccagctt cagggtgggt cccaggtcgt caaggtgggc actgcctgga aacaagtata
     6721 tcaattcctt ggagttccat atgctgcccc acccttggca gaaaaccgtt tccaggcacc
     6781 agaggtcttg aattggacag gctcctggga tgccaccaag ctaaggtcca gctgctggca
     6841 gccaggtacc cgtaccccaa cacctcctca aatcagtgaa gactgcttat atctcaacgt
     6901 gtttgtccct gagaacctgg tctccaatgc atcagttctg gtgttcttcc acaatactgt
     6961 ggagatggaa ggcagtggag gtcagctgaa cattgatggc tccattctgg ctgcagttgg
     7021 caacctcatc gtagtcactg ctaactacag actgggggtc tttggcttct tgagctcagg
     7081 ctctgatgaa gtagctggca actgggggct actggaccaa gtggcagctc taacatgggt
     7141 gcagacccac attggagcat ttggtgggga ccctcaacgt gtgacactgg cagcagaccg
     7201 tggtggggct gatgtggcca gcatacacct tctcatcacc agacctacca gactccagct
     7261 cttcaggaag gctctgctta tgggaggctc agcattgtca ccggctgcaa ttatcagccc
     7321 agatagggcc caacagcaag ctgcagcctt ggcaaaggag gttggatgcc ctaactcatc
     7381 cgtccaggaa gtggtctcct gcttccgcca gaagcctgcc aacattctca atgaagctca
     7441 gaccaagctc ctggctgtga gtggcccttt ccactactgg ggtcctgtgg tcgatggcca
     7501 gtacctacgt gaacttcctt ccagacggtt gaagaggcct ctgccagtga aggtggatct
     7561 gctcatagga ggttctcagg acgatgggct tatcaacagg gcaaaggctg tgaagcaatt
     7621 tgaagaaagc caaggccgaa ccaacagtaa aacagccttt taccaggcac tgcagaattc
     7681 actaggcggt gaggactcag acgcccgcat ccttgctgct gccatatggt attactccct
     7741 ggagcactcc acagatgact atgcctcctt ctctcgggca ctggagaatg ccacccggga
     7801 ctactttatc atctgtccca ttgtcaacat ggccagcctc tgggcaagga gaacccgtgg
     7861 aaatgtcttc atgtaccatg tccccgaaag ctatggccat ggcagcctag aattgctggc
     7921 agatgttcag tatgcttttg gactgccctt ctactctgcc taccagggct acttctcaat
     7981 ggaggagcag agcctctctc tgaaagtcat gcagtatttc tccaacttca tcagatcagg
     8041 aaatcctaac tacccacatg agttctcaca gaaagcagct gaatttgcca caccctggcc
     8101 agactttgtt cctggagctg gaggagagag ctacaaggag ctcagtgccc agcttcccaa
     8161 tcggcagggc ctcaaaaaag ctgactgttc cttctggtcc aagtacatcc agactctgaa
     8221 ggatgcagat ggagccaagg atggacagtt aaccaagagt ggagaggagg acttggaagt
     8281 ggggcctggg tcagaagaag acttctcagg ctcactggag cctgtcccca agagctacag
     8341 caaatgacca accctgaggt ctcccccacc ttcgctgcca gttctacccc aaactgcctc
     8401 ttgtggtggt tttactatat acagcaacca tttgctttca ataaagtatt tgcaaacaac
     8461 a
//
