LOCUS       NM_173132               1367 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus testin gene (Testin), mRNA.
ACCESSION   NM_173132
VERSION     NM_173132.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Su,L., Wang,Z., Xie,S., Hu,D., Cheng,Y.C., Mruk,D.D. and Guan,Y.
  TITLE     Testin regulates the blood-testis barrier via disturbing
            occludin/ZO-1 association and actin organization
  JOURNAL   J Cell Physiol 235 (9), 6127-6138 (2020)
   PUBMED   31975378
  REMARK    GeneRIF: Testin regulates the blood-testis barrier via disturbing
            occludin/ZO-1 association and actin organization.
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Grima,J., Zhu,L.J., Zong,S.D., Catterall,J.F., Bardin,C.W. and
            Cheng,C.Y.
  TITLE     Rat testin is a newly identified component of the junctional
            complexes in various tissues whose mRNA is predominantly expressed
            in the testis and ovary
  JOURNAL   Biol Reprod 52 (2), 340-355 (1995)
   PUBMED   7711203
REFERENCE   3  (bases 1 to 1367)
  AUTHORS   Cheng,C.Y., Morris,I. and Bardin,C.W.
  TITLE     Testins are structurally related to the mouse cysteine proteinase
            precursor but devoid of any protease/anti-protease activity
  JOURNAL   Biochem Biophys Res Commun 191 (1), 224-231 (1993)
   PUBMED   8447824
REFERENCE   4  (bases 1 to 1367)
  AUTHORS   Cheng,C.Y., Grima,J., Stahler,M.S., Lockshin,R.A. and Bardin,C.W.
  TITLE     Testins are structurally related Sertoli cell proteins whose
            secretion is tightly coupled to the presence of germ cells
  JOURNAL   J Biol Chem 264 (35), 21386-21393 (1989)
   PUBMED   2592382
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from U16858.1.
            
            On Aug 30, 2012 this sequence version replaced NM_173132.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U16858.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1367              U16858.1           5-1371
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     gene            1..1367
                     /gene="Testin"
                     /note="testin gene"
                     /db_xref="GeneID:286916"
                     /db_xref="RGD:708447"
     exon            1..81
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            82..218
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1094
                     /gene="Testin"
                     /note="testin-2; CMB-23"
                     /codon_start=1
                     /product="testin-2 precursor"
                     /protein_id="NP_775155.1"
                     /db_xref="GeneID:286916"
                     /db_xref="RGD:708447"
                     /translation="MIAVLFLAILCLEVDSTAPTPDPSLDVEWNEWRTKHGKTYNMNE
                     ERLKRAVWEKNFKMIELHNWEYLEGRHDFTMAMNAFGDLTNIEFVKMMTGFQRQKIKK
                     THIFQDHQFLYVPKRVDWRQLGYVTPVKNQGHCASSWAFSATGSLEGQMFRKTERLIP
                     LSEQNLLDCMGSNVTHGCSGGFMQYAFQYVKDNGGLATEESYPYRGQGRECRYHAENS
                     AANVRDFVQIPGSEEALMKAVAKVGPISVAVDASHGSFQFYGSGIYYEPQCKRVHLNH
                     AVLVVGYGFEGEESDGNSFWLVKNSWGEEWGMKGYMKLAKDWSNHCGIATYSTYPIV"
     exon            219..341
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            342..488
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            489..713
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            714..876
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            877..994
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1364
                     /gene="Testin"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agctgaaaca gatctgtaga acccaagatt ctctgaaact caagtctgag gagatcatct
       61 gaggagcagc agacatccca ggtgtatgag acatgattgc tgttctcttc ctagcgattc
      121 tctgcttgga agttgactca actgctccaa caccagatcc cagtttggat gttgagtgga
      181 atgaatggag gacaaaacat gggaaaacat acaacatgaa tgaagaaaga ctgaagagag
      241 ctgtgtggga aaagaatttt aaaatgattg aactgcataa ttgggaatac cttgagggga
      301 ggcatgactt caccatggca atgaatgcct ttggtgactt gaccaatata gaatttgtga
      361 aaatgatgac aggctttcaa aggcagaaga tcaagaagac gcacatattc caggatcatc
      421 agtttcttta tgtcccaaaa cgtgtggatt ggagacaact tggctatgtg actcctgtga
      481 agaatcaggg tcattgtgcc tctagttggg cttttagtgc aactgggtcc ctagaaggac
      541 aaatgttcag gaaaacagaa agactgatcc ctctgagtga gcagaaccta ctggactgca
      601 tgggatctaa tgttacccat ggttgcagcg gtggcttcat gcagtatgcc ttccaatacg
      661 tgaaagacaa tggcggcctt gcaactgagg aatcctatcc ttacagagga caaggtagag
      721 agtgcaggta ccacgctgag aattctgcgg ctaatgtcag agattttgtg caaatcccag
      781 gaagcgagga agcccttatg aaggcagtgg ctaaagtggg gcccatctct gttgcagttg
      841 atgccagcca tggttccttc cagttctacg gtagtggcat ctattatgag ccacagtgta
      901 aaagggttca cctaaatcat gctgttctag tggtcggcta tggctttgaa ggagaagaat
      961 cagatggcaa cagtttttgg ctcgtcaaga acagctgggg tgaggaatgg ggcatgaagg
     1021 gctacatgaa gcttgccaaa gactggagca accactgcgg gattgctaca tattccacat
     1081 accccattgt atgagctact gcacagtgac aaggcaggag tggactggaa tgtactgagc
     1141 agttttctcc ttccacagac cagcctctgc tgtggggcaa aggggacagg ggtagtgaca
     1201 gctgagtcac tgaggatcca cacctgatga gaattaaact cataaacatt agtgccatta
     1261 acagctttgt aggacagact ctcatataaa aaaaaaatga attttcagtt taaaatgctg
     1321 tacaaattta catattcaaa ataaagctta atttcacaac ggtaaaa
//
