LOCUS       NM_001388503            2995 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus thymine-DNA glycosylase (Tdg), transcript variant
            1, mRNA.
ACCESSION   NM_001388503
VERSION     NM_001388503.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Liu,Y., Sun,L.Y., Singer,D.V., Ginnan,R., Zhao,W.,
            Jourd'heuil,F.L., Jourd'heuil,D., Long,X. and Singer,H.A.
  TITLE     Thymine DNA glycosylase is a key regulator of CaMKIIgamma
            expression and vascular smooth muscle phenotype
  JOURNAL   Am J Physiol Heart Circ Physiol 317 (5), H969-H980 (2019)
   PUBMED   31518169
  REMARK    GeneRIF: Thymine DNA glycosylase plays a key role in regulating
            CaMKIIgamma expression in vascular smooth muscle cells through
            promoter cytosine methylation/demethylation.
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Maiti,A., Noon,M.S., MacKerell,A.D. Jr., Pozharski,E. and
            Drohat,A.C.
  TITLE     Lesion processing by a repair enzyme is severely curtailed by
            residues needed to prevent aberrant activity on undamaged DNA
  JOURNAL   Proc Natl Acad Sci U S A 109 (21), 8091-8096 (2012)
   PUBMED   22573813
REFERENCE   3  (bases 1 to 2995)
  AUTHORS   Zhang,L., Lu,X., Lu,J., Liang,H., Dai,Q., Xu,G.L., Luo,C., Jiang,H.
            and He,C.
  TITLE     Thymine DNA glycosylase specifically recognizes
            5-carboxylcytosine-modified DNA
  JOURNAL   Nat Chem Biol 8 (4), 328-330 (2012)
   PUBMED   22327402
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2995)
  AUTHORS   Maiti,A. and Drohat,A.C.
  TITLE     Thymine DNA glycosylase can rapidly excise 5-formylcytosine and
            5-carboxylcytosine: potential implications for active demethylation
            of CpG sites
  JOURNAL   J Biol Chem 286 (41), 35334-35338 (2011)
   PUBMED   21862836
REFERENCE   5  (bases 1 to 2995)
  AUTHORS   He,Y.F., Li,B.Z., Li,Z., Liu,P., Wang,Y., Tang,Q., Ding,J., Jia,Y.,
            Chen,Z., Li,L., Sun,Y., Li,X., Dai,Q., Song,C.X., Zhang,K., He,C.
            and Xu,G.L.
  TITLE     Tet-mediated formation of 5-carboxylcytosine and its excision by
            TDG in mammalian DNA
  JOURNAL   Science 333 (6047), 1303-1307 (2011)
   PUBMED   21817016
REFERENCE   6  (bases 1 to 2995)
  AUTHORS   Hardeland,U., Steinacher,R., Jiricny,J. and Schar,P.
  TITLE     Modification of the human thymine-DNA glycosylase by ubiquitin-like
            proteins facilitates enzymatic turnover
  JOURNAL   EMBO J 21 (6), 1456-1464 (2002)
   PUBMED   11889051
REFERENCE   7  (bases 1 to 2995)
  AUTHORS   Missero,C., Pirro,M.T., Simeone,S., Pischetola,M. and Di Lauro,R.
  TITLE     The DNA glycosylase T:G mismatch-specific thymine DNA glycosylase
            represses thyroid transcription factor-1-activated transcription
  JOURNAL   J Biol Chem 276 (36), 33569-33575 (2001)
   PUBMED   11438542
REFERENCE   8  (bases 1 to 2995)
  AUTHORS   Niederreither,K., Harbers,M., Chambon,P. and Dolle,P.
  TITLE     Expression of T:G mismatch-specific thymidine-DNA glycosylase and
            DNA methyl transferase genes during development and tumorigenesis
  JOURNAL   Oncogene 17 (12), 1577-1585 (1998)
   PUBMED   9794235
REFERENCE   9  (bases 1 to 2995)
  AUTHORS   Marietta,C., Palombo,F., Gallinari,P., Jiricny,J. and Brooks,P.J.
  TITLE     Expression of long-patch and short-patch DNA mismatch repair
            proteins in the embryonic and adult mammalian brain
  JOURNAL   Brain Res Mol Brain Res 53 (1-2), 317-320 (1998)
   PUBMED   9473709
REFERENCE   10 (bases 1 to 2995)
  AUTHORS   Neddermann,P., Gallinari,P., Lettieri,T., Schmid,D., Truong,O.,
            Hsuan,J.J., Wiebauer,K. and Jiricny,J.
  TITLE     Cloning and expression of human G/T mismatch-specific thymine-DNA
            glycosylase
  JOURNAL   J Biol Chem 271 (22), 12767-12774 (1996)
   PUBMED   8662714
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360172.1385494.1,
                                           SRR26360190.2949653.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               JAXUCZ010000007.1  22984901-22985100   c
            201-331             JAXUCZ010000007.1  22976186-22976316   c
            332-573             JAXUCZ010000007.1  22973611-22973852   c
            574-643             JAXUCZ010000007.1  22972867-22972936   c
            644-779             JAXUCZ010000007.1  22970752-22970887   c
            780-862             JAXUCZ010000007.1  22970510-22970592   c
            863-957             JAXUCZ010000007.1  22970334-22970428   c
            958-1129            JAXUCZ010000007.1  22968084-22968255   c
            1130-1255           JAXUCZ010000007.1  22967476-22967601   c
            1256-2995           JAXUCZ010000007.1  22965388-22967127   c
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q13"
     gene            1..2995
                     /gene="Tdg"
                     /note="thymine-DNA glycosylase"
                     /db_xref="GeneID:114521"
                     /db_xref="RGD:620959"
     exon            1..200
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..1410
                     /gene="Tdg"
                     /EC_number="3.2.2.-"
                     /note="isoform 1 is encoded by transcript variant 1; G/T
                     mismatch-specific thymine DNA glycosylase"
                     /codon_start=1
                     /product="G/T mismatch-specific thymine DNA glycosylase
                     isoform 1"
                     /protein_id="NP_001375432.1"
                     /db_xref="GeneID:114521"
                     /db_xref="RGD:620959"
                     /translation="MDAEAARSYSLEQVQALYSFPFQQMMAEAPNMADVAGQQMPAEA
                     PAQDPVPEAPKRRKRKTRAAEAQDPVEPKKPAASKKSGKSTKSKEKQEKITDTFKVKR
                     KVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLF
                     MSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKY
                     QPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFP
                     RAQDKVHYYIKLKDLRDQLKGIERSTDVQEVQYTFDLQLAQEDAKRTAVKEEKYDPGY
                     EAAYGGACGENPCNGEPCGFASNGLTANSAELGGESAPSDVPNGQWMAQSFAEQIPSF
                     NNCGTGEQEAGSHAGSHA"
     exon            201..331
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            332..573
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            574..643
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            644..779
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            780..862
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            863..957
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1129
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1255
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..2995
                     /gene="Tdg"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagggggac ggaagtgcac tgtggactcg ccgcggtgat gtagcagcgt gggaggggcc
       61 gaggccagcg ccatccgtcc accggccgcc cggatcctgt ggtgagtccg caggccgatc
      121 ggcagagcgg gcgcccgcgg gctgggcagg agtagccgca ctctcagcgg cgccgccatg
      181 gacgcggagg ccgcgcgcag ctactctctg gagcaagttc aagctctgta ttcatttcca
      241 ttccaacaaa tgatggcgga ggctccgaac atggcagacg tggccggaca gcagatgcct
      301 gcagaagctc ctgcccagga tcctgtgcca gaggctccaa agagaaggaa aaggaaaacc
      361 agggcagcag aggcccagga cccagtggag cccaaaaagc ctgctgcctc aaagaaatct
      421 ggcaagtcca caaaatcaaa agaaaagcaa gagaaaatca cagacacctt taaagtgaaa
      481 aggaaagtgg accggttcaa cggtgtctct gaagctgagc ttctgaccaa gactctccct
      541 gacattttga ccttcaatct ggatattgta attattggca tcaacccagg attaatggcc
      601 gcttacaaag ggcatcatta ccctgggccc ggaaatcact tctggaagtg tctgttcatg
      661 tctgggttga gcgaggtgca gctgaatcac atggatgacc acaccttacc tggaaaatac
      721 ggcatcggat tcaccaacat ggtggaacgg acaacgccag gcagcaagga tctgtctagt
      781 aaagaattcc gggaaggagg gcgtatccta gtgcagaaac tacagaaata tcagccacga
      841 atagcggtgt ttaatggaaa atgtatttat gagattttca gtaaagaagt ttttggagta
      901 aaggttaaga acttggaatt tgggcttcaa ccgcacaaga tcccagacac agaaactctc
      961 tgctacgtca tgccgtcctc cagtgcccgg tgcgctcagt tccctcgggc gcaggacaaa
     1021 gtccattact acattaagct gaaggacttg cgagaccagc tgaagggcat cgagcgcagc
     1081 acggacgttc aggaggtgca gtacacgttc gacctgcagc tcgcacaaga ggacgcaaag
     1141 aggacggctg ttaaggaaga aaagtatgac ccaggctatg aggcagcgta tggcggtgcc
     1201 tgtggtgaaa acccgtgtaa tggggaacct tgtggctttg cttcaaatgg gctaacagcc
     1261 aacagtgcgg agctgggagg agaatcagct ccgagcgacg ttccaaacgg gcagtggatg
     1321 gcgcagtcgt ttgcagagca gatcccttct ttcaataatt gtggcacagg agagcaggaa
     1381 gcaggaagcc atgcaggaag ccatgcttag cgaagatgcc tcagctctgg ctcagcgctg
     1441 cagttttagt gcagttgtca ggacacagag cctcagtttg ctaactgagg agttttagta
     1501 ttaatagtac tttacgctag tggtgtaact atatatggag cagttatatg gtatgaataa
     1561 tctgtgtagt ttggaaacca aaagtagggt gttttgtact tgagttttgt atttgaattg
     1621 cccatcattc cagcttttta tatactatat ttcatgtatg aagaaactgg ttttcttcga
     1681 ggagtcactt caatctgtga ttttagaaaa gcaacagtct gaatatttaa agttgattct
     1741 tcactgtgca tacctatcta tgccctcatc cctttagatg cctcagaaag gcatgctagt
     1801 aagagtcgac tcggtaaaaa tgcagctgtc agtctccagg tttgaagctg actaaccctc
     1861 cgtggaatca aagctggggc tgaaccagaa cccccttact ctcccagagg tgtgtgtggc
     1921 agatggttgg taagagaaga acggtgtggc catacttggt ggggattttg agccctgctg
     1981 ttgacctctg cctcttgtgt gttcgcccct cagaggcaga cacttctgct gagctggtag
     2041 gctcctgaga acacagtagg acttgtacag cctgccacgc gtctgtttta ctgaatccct
     2101 gtcagtgttc ctcactgttc tgattttaac cttgacatgg aaagtgataa gaaacgaact
     2161 ttccagtgag caatgcgagg gcgcaggtgc ttaatagtcc tgttccaact ggtgctatgt
     2221 acgtgggatg tcctgataca ggacgtttct tgctaggtaa agctaatcat tgatgaatga
     2281 tttaaaaatt attgtcactg ctattgatta ctgtggacta agtgagcctc atgaaaaagt
     2341 tgattggaat ttgtgccttt gtggttgcta atgcattttc cacagggcta cagacagccc
     2401 accagggctg gtggatatta accgtgggta ttcagaaatg cctggtgcat ccacgcctga
     2461 ccactctcga gttctctgac ttgatggtac tcctggagct cgctggtttt tcaatatgaa
     2521 atgtccacga gggttttgta tttcacagag gctatgtagc ctgttctatg aataatgaga
     2581 gaagcagttt cttggtagaa atttttaaca tgcctttagc tgtggcaatg atggactttc
     2641 cttttccttc tgtctgggtc aggctgtgta caagtcatgt gatttgaaag gggcactgca
     2701 ctgctgttta catgggcatt tcatgcgggt gctttgaagt gccttgcatc agggattaga
     2761 atcaattgaa ttattttttt tcatgggact gtaaagcatg tagtattgcc ttggtatgta
     2821 actattgtag ctttaccatg gagaaatacc tttaaattat gactggagag tgggtgcgtg
     2881 gaccctctga gtactgtcct ggtgcttttc cttgaacatg gtgagtagtg agtgctgtgc
     2941 accctgtact gacagggtgg gatttgtttt cattaaataa tactgagatt ttaaa
//
