LOCUS       NM_001399216            2579 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus threonyl-tRNA synthetase 1 (Tars1), transcript
            variant 1, mRNA.
ACCESSION   NM_001399216 XM_006232068
VERSION     NM_001399216.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Chen,Y., Ruan,Z.R., Wang,Y., Huang,Q., Xue,M.Q., Zhou,X.L. and
            Wang,E.D.
  TITLE     A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and
            editing activities
  JOURNAL   Nucleic Acids Res 46 (7), 3643-3656 (2018)
   PUBMED   29579307
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Fang,P., Yu,X., Jeong,S.J., Mirando,A., Chen,K., Chen,X., Kim,S.,
            Francklyn,C.S. and Guo,M.
  TITLE     Structural basis for full-spectrum inhibition of translational
            functions on a tRNA synthetase
  JOURNAL   Nat Commun 6, 6402 (2015)
   PUBMED   25824639
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2579)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   4  (bases 1 to 2579)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   5  (bases 1 to 2579)
  AUTHORS   Freist,W. and Gauss,D.H.
  TITLE     Threonyl-tRNA synthetase
  JOURNAL   Biol Chem Hoppe Seyler 376 (4), 213-224 (1995)
   PUBMED   7626230
  REMARK    Review article
REFERENCE   6  (bases 1 to 2579)
  AUTHORS   Ogata,K., Kurahashi,A., Nishiyama,C. and Terao,K.
  TITLE     Presence of role of the 5SrRNA-L5 protein complex (5SRNP) in the
            threonyl- and histidyl-tRNA synthetase complex in rat liver cytosol
  JOURNAL   Biochim Biophys Acta 1218 (3), 388-400 (1994)
   PUBMED   8049265
REFERENCE   7  (bases 1 to 2579)
  AUTHORS   Cruzen,M.E. and Arfin,S.M.
  TITLE     Nucleotide and deduced amino acid sequence of human threonyl-tRNA
            synthetase reveals extensive homology to the Escherichia coli and
            yeast enzymes
  JOURNAL   J Biol Chem 266 (15), 9919-9923 (1991)
   PUBMED   2033077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Jan 4, 2022 this sequence version replaced XM_006232068.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643290.7505.1,
                                           SRR26643287.4954.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760393,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               JAXUCZ010000002.1  62114641-62114802   c
            163-243             JAXUCZ010000002.1  62112652-62112732   c
            244-434             JAXUCZ010000002.1  62109747-62109937   c
            435-558             JAXUCZ010000002.1  62108731-62108854   c
            559-680             JAXUCZ010000002.1  62108386-62108507   c
            681-798             JAXUCZ010000002.1  62107829-62107946   c
            799-863             JAXUCZ010000002.1  62107569-62107633   c
            864-942             JAXUCZ010000002.1  62107413-62107491   c
            943-1089            JAXUCZ010000002.1  62106678-62106824   c
            1090-1188           JAXUCZ010000002.1  62105973-62106071   c
            1189-1355           JAXUCZ010000002.1  62104480-62104646   c
            1356-1518           JAXUCZ010000002.1  62102869-62103031   c
            1519-1656           JAXUCZ010000002.1  62102650-62102787   c
            1657-1734           JAXUCZ010000002.1  62102272-62102349   c
            1735-1835           JAXUCZ010000002.1  62102044-62102144   c
            1836-1940           JAXUCZ010000002.1  62101850-62101954   c
            1941-2013           JAXUCZ010000002.1  62100430-62100502   c
            2014-2128           JAXUCZ010000002.1  62098433-62098547   c
            2129-2579           JAXUCZ010000002.1  62095978-62096428   c
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q16"
     gene            1..2579
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /note="threonyl-tRNA synthetase 1"
                     /db_xref="GeneID:294810"
                     /db_xref="RGD:1359527"
     exon            1..162
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..2277
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /EC_number="6.1.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     threonyl-tRNA synthetase, cytoplasmic; thrRS;
                     threonine--tRNA ligase, cytoplasmic; threonine--tRNA
                     ligase 1, cytoplasmic"
                     /codon_start=1
                     /product="threonine--tRNA ligase 1, cytoplasmic isoform 1"
                     /protein_id="NP_001386145.1"
                     /db_xref="GeneID:294810"
                     /db_xref="RGD:1359527"
                     /translation="MSEEKASSPSGKMDGEKPVDASEEKRKEGGKKRNKDGGGDGGRA
                     ELNPWPEYINTRLDMYHKLKAEHDSILAEKAAKDSKPIKVTLPDGKQVDAESWKTTPY
                     QIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIM
                     GEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERL
                     EVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKI
                     HKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFF
                     HELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYS
                     ENMFSFEVEKEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTG
                     LTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDI
                     EIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQL
                     PIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVP
                     VGPTCDEYAQKVRQEFHDAKFMVDIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKAS
                     GTVNIRTRDNKVHGERTVGETVERLQQLKQLRSKQAEEEF"
     exon            163..243
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            244..434
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            435..558
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            559..680
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            681..798
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            799..863
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            864..942
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1089
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1188
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1355
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1518
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1656
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1734
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1835
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1940
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2013
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2128
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2579
                     /gene="Tars1"
                     /gene_synonym="Tars"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgggccacg tggcgcacgc gcagtgcaga attgcaagtc ttcccccgcc ggctttaggg
       61 tcttttgtgc cgtgtagttc ttggattttg tctctcagcg gtccttctat gtcggaggag
      121 aaggctagca gcccttcggg gaagatggac ggcgagaagc cggtggatgc cagcgaggag
      181 aagcgaaaag aaggaggcaa gaagaggaac aaagatggag gtggagacgg aggtcgagca
      241 gagttgaatc cctggccgga gtacattaac acacgccttg atatgtacca caagctcaaa
      301 gcagagcatg attccatcct ggctgaaaag gcagcaaaag acagcaagcc gattaaagtc
      361 accctgcccg acggcaagca agtggatgct gagtcctgga aaaccacgcc gtatcagatt
      421 gcatgtggca ttagccaggg cctggctgac aacaccgttg ttgctaaggt gaacaaggtt
      481 gtttgggacc tggaccgccc gctggagaca gactgcactc tggagctgct caagtttgag
      541 gacgaggaag cacaggcagt atattggcac tctagtgccc acataatggg tgaggccatg
      601 gaaagagtct acggtggatg cttatgttac ggccctccaa tagaaaacgg cttctattat
      661 gatatgtacc ttgaagaagg gggtgtgtcc agcaatgatt tctcttccct ggaaactttg
      721 tgtaagaaaa tcattaaaga gaaacaaact tttgaaagat tggaagttaa aaaggaaaca
      781 ttactagaaa tgttcaagta caacaagttc aagtgccgga tattgaatga gaaagtgaat
      841 actccgacca cgactgtcta tagatgtggc cccttgatag atctctgccg tggtcctcac
      901 gtccgacaca ctggcaagat taagactctg aaaatacaca agaattcctc cacgtactgg
      961 gaaggcaaag cggacatgga aacgctccag aggatttatg ggatttcatt ccccgacccc
     1021 aaactactga aagagtggga gaagttccaa gaggaagcca agaaccgaga ccataggaag
     1081 attgggcggg accaagaact atatttcttc cacgaactca gccctggaag ttgctttttc
     1141 ctgccaaaag gagcctacat ttataataca ctgatggaat tcatcaggag tgaatatagg
     1201 aaaagagggt tccaggaggt cgtcaccccc aacatcttca atagccggct ctggatgacc
     1261 tctggccact ggcagcacta cagtgaaaac atgttctcct ttgaggtgga gaaggagcag
     1321 tttgccctca agcccatgaa ctgcccagga cactgcctga tgttcgatca tcggccgagg
     1381 tcctggcgag agctgcccct gcggttagct gattttggag tgcttcacag gaacgagctc
     1441 tcaggggctc tcactggact cacacgggtc cgaagattcc agcaggatga cgcacacata
     1501 ttctgtgcca tggagcagat tgaagatgag atcaaaggtt gtttggattt tcttcgcacg
     1561 gtatatagtg tctttggatt ttcatttaaa ctgaatcttt ctactcgccc agaaaaattc
     1621 ctgggagata ttgaaatatg gaaccaagct gagaaacaac ttgaaaacag tttgaacgaa
     1681 tttggtgaga agtgggagct aaatcctggc gatggagcct tctatggtcc aaagattgat
     1741 atacagataa aggacgctat tggccgctac caccagtgtg cgaccatcca gctggacttt
     1801 cagttgccca tcaggtttaa cctcacttac gtcagccatg atggcgacga taagaagagg
     1861 ccagtgatcg ttcaccgagc catcctgggg tcagtggaga gaatgattgc catcctcacg
     1921 gaaaactatg ggggcaaatg gcctttctgg ctctctcctc gccaggtgat ggtggtccca
     1981 gtgggaccga cctgtgatga atatgcccaa aaggtacggc aagaattcca cgatgctaag
     2041 ttcatggtgg acattgacct ggatccaggc tgtaccttga ataagaagat cagaaatgca
     2101 cagttggcac agtataactt catccttgtt gttggtgaaa aagagaaagc cagtggcact
     2161 gtgaacatcc gcacgagaga caacaaggtc cacggagagc ggactgtggg ggaaacagtg
     2221 gagcggctgc agcagctcaa gcagttgcgc agtaagcagg cagaggagga gttttaacag
     2281 gcacatggcc cgagctggct cagtgtagag gaagatgggt gcccatcacg tgaccttgtg
     2341 cactgggaaa catggcttca tgatgagcaa ggagtggttc tgccgagtct ttgtggatca
     2401 gcctttctgg tctggacaag tcttagagaa tgctgttaga tgggggctaa gaagagaacc
     2461 attaaatgtc tctctccaaa atggaaaatc atggaggatg ttttctgtac ccaggttatt
     2521 ttgtatgaat tcaatcaatt agaaaataaa aaaattttaa atgtgcctaa aaaaaaaaa
//
