LOCUS       NM_053325               1461 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus synaptotagmin 8 (Syt8), mRNA.
ACCESSION   NM_053325
VERSION     NM_053325.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Monterrat,C., Boal,F., Grise,F., Hemar,A. and Lang,J.
  TITLE     Synaptotagmin 8 is expressed both as a calcium-insensitive soluble
            and membrane protein in neurons, neuroendocrine and endocrine cells
  JOURNAL   Biochim Biophys Acta 1763 (1), 73-81 (2006)
   PUBMED   16386321
  REMARK    GeneRIF: Endogenous cytosolic syt8 and transiently expressed
            syt8-C2AB-eGFP did not translocate upon a raise in cytosolic
            calcium in living cells.
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Hutt,D.M., Baltz,J.M. and Ngsee,J.K.
  TITLE     Synaptotagmin VI and VIII and syntaxin 2 are essential for the
            mouse sperm acrosome reaction
  JOURNAL   J Biol Chem 280 (21), 20197-20203 (2005)
   PUBMED   15774481
REFERENCE   3  (bases 1 to 1461)
  AUTHORS   Gut,A., Kiraly,C.E., Fukuda,M., Mikoshiba,K., Wollheim,C.B. and
            Lang,J.
  TITLE     Expression and localisation of synaptotagmin isoforms in endocrine
            beta-cells: their function in insulin exocytosis
  JOURNAL   J Cell Sci 114 (Pt 9), 1709-1716 (2001)
   PUBMED   11309201
REFERENCE   4  (bases 1 to 1461)
  AUTHORS   Kishore,B.K., Wade,J.B., Schorr,K., Inoue,T., Mandon,B. and
            Knepper,M.A.
  TITLE     Expression of synaptotagmin VIII in rat kidney
  JOURNAL   Am J Physiol 275 (1), F131-F142 (1998)
   PUBMED   9689015
REFERENCE   5  (bases 1 to 1461)
  AUTHORS   Li,C., Ullrich,B., Zhang,J.Z., Anderson,R.G., Brose,N. and
            Sudhof,T.C.
  TITLE     Ca(2+)-dependent and -independent activities of neural and
            non-neural synaptotagmins
  JOURNAL   Nature 375 (6532), 594-599 (1995)
   PUBMED   7791877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 5, 2020 this sequence version replaced NM_053325.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.5158.1,
                                           SRR26643299.5160.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06621351, SAMN11044071
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               JAXUCZ010000001.1  207017602-207017719
            119-297             JAXUCZ010000001.1  207020723-207020901
            298-467             JAXUCZ010000001.1  207021003-207021172
            468-566             JAXUCZ010000001.1  207021563-207021661
            567-725             JAXUCZ010000001.1  207021789-207021947
            726-893             JAXUCZ010000001.1  207022021-207022188
            894-999             JAXUCZ010000001.1  207022326-207022431
            1000-1133           JAXUCZ010000001.1  207022532-207022665
            1134-1461           JAXUCZ010000001.1  207022749-207023076
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..1461
                     /gene="Syt8"
                     /note="synaptotagmin 8"
                     /db_xref="GeneID:60566"
                     /db_xref="RGD:68399"
     exon            1..118
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            119..297
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..1367
                     /gene="Syt8"
                     /note="sytVIII; synaptotagmin VIII"
                     /codon_start=1
                     /product="synaptotagmin-8"
                     /protein_id="NP_445777.2"
                     /db_xref="GeneID:60566"
                     /db_xref="RGD:68399"
                     /translation="MRHVSNPLSTSAPVDATAGPNLIPDLITKIPWPRWILFIAILAA
                     GVLLVSCLLCVICYCCHRQRHRKQPKDKETVGLGSARNSTTTHLVQPDVECLEPCSGG
                     DQPWGQLLLSLEYDFGSQEIRVGLRQAKNLKAEGTADPYARVSVSTQAGRRHETKVHR
                     GTLCPMFEETCCFLVPPAELPKATLKVQLLDFKRFSEHEPLGELQLPLGTVDLQHVLE
                     SWYQLGPPGSTESEQMGELCFSLRYVPSSGRLTVVILEARGLNPGLAEAYVKVQLILN
                     QRKWKKNKTSSKKGTTNPYFNEAFVFLVPVSQLQSMDLVLAVWARGLQLLAEPVGKVL
                     LGPRASGQPLQHWADMLAHARRPIAQWHHLRSPREVDRALALQPRLPLLRPRS"
     exon            298..467
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            468..566
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            567..725
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            726..893
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            894..999
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1133
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1461
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcggtctc tgcagtgggc ttcaggtgct gcctgagagt ggacatccag cctcccagtg
       61 gctagggtct gagcgagcag gccagagccc accaatcaca ggaagcagta ggcaccaggc
      121 taccttcttg cttagtgtgg actcagtact ggctgtggcc tggattccac aattatttcc
      181 tcgaagccga taggagcacg aagatgcggc atgtctcaaa ccccctcagc acctctgccc
      241 cagttgatgc caccgctggg cccaatctca ttccagacct cattaccaag atcccctggc
      301 ctcgctggat actctttatt gccatccttg ctgctggagt cctcttagtc tcctgtctgc
      361 tctgtgtcat ctgttactgt tgccaccgcc aacgccacag gaagcaaccc aaggataaag
      421 agactgtggg cctgggcagt gctcggaaca gcaccactac tcacctggtt caaccagatg
      481 tggagtgcct agagccctgc tctggagggg accaaccgtg ggggcaactg ctgctctccc
      541 tggagtatga ttttggaagc caggagatta gagtgggcct gaggcaggct aaaaatctga
      601 aggctgaggg cacagcagac ccctacgccc gtgtcagtgt ttccacccag gccgggagaa
      661 gacatgagac aaaagtgcac cgtgggacac tctgtcccat gtttgaagag acatgctgct
      721 tcctggtccc accagcagag ctgcccaaag ccactctgaa ggtgcagcta ttggacttca
      781 agcggttttc agaacatgag ccactggggg agctccagct acccctgggc actgtggacc
      841 ttcagcatgt cctggagagc tggtaccagc taggccctcc aggttccact gagtctgagc
      901 agatggggga gctgtgcttc tcactacgct atgtgcccag ctcaggccgc ctgactgtgg
      961 tcatactgga ggctcggggc ctgaatccgg ggcttgcaga ggcctatgtg aaggtccagc
     1021 ttatattaaa ccagagaaag tggaagaaga acaaaacatc ctccaagaag ggcacaacta
     1081 acccctactt caacgaggcc ttcgtcttcc tggttcccgt tagccagctc cagagcatgg
     1141 acctggtgct ggctgtctgg gcccggggcc tgcagctcct ggctgagcct gtgggcaaag
     1201 tgttgctggg tccccgggct tcaggccaac ccctacagca ctgggcagac atgctggccc
     1261 atgctcggcg gcccatcgcc cagtggcacc acctgcggtc acccagggaa gtggatcgtg
     1321 ctctggccct gcagcctcgt cttcccctgc ttaggcctcg ctcctgaaag aaccgtaggt
     1381 ctcagctttc cccagtggag agctgtggcc atttgtgcag gctcttcgga tctctctgta
     1441 ataaatgcat cctctatcac a
//
