LOCUS       NM_031693               3877 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus synaptotagmin 4 (Syt4), mRNA.
ACCESSION   NM_031693
VERSION     NM_031693.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Stucchi,R., Plucinska,G., Hummel,J.J.A., Zahavi,E.E., Guerra San
            Juan,I., Klykov,O., Scheltema,R.A., Altelaar,A.F.M. and
            Hoogenraad,C.C.
  TITLE     Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca2+/CaM
            Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to
            Postsynaptic Sites
  JOURNAL   Cell Rep 24 (3), 685-700 (2018)
   PUBMED   30021165
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Bharat,V., Siebrecht,M., Burk,K., Ahmed,S., Reissner,C.,
            Kohansal-Nodehi,M., Steubler,V., Zweckstetter,M., Ting,J.T. and
            Dean,C.
  TITLE     Capture of Dense Core Vesicles at Synapses by JNK-Dependent
            Phosphorylation of Synaptotagmin-4
  JOURNAL   Cell Rep 21 (8), 2118-2133 (2017)
   PUBMED   29166604
  REMARK    GeneRIF: neuronal activity increased dense core vesicle capture via
            JNK-dependent phosphorylation of the S135 site of Synaptotagmin-4
REFERENCE   3  (bases 1 to 3877)
  AUTHORS   Tratnjek,L., Glavan,G., Visnjar,T. and Zivin,M.
  TITLE     Upregulation and axonal transport of synaptotagmin-IV in the
            direct-pathway medium spiny neurons in hemi-parkinsonian rats
            induced by dopamine D1 receptor stimulation
  JOURNAL   Eur J Neurosci 43 (7), 885-898 (2016)
   PUBMED   26750488
REFERENCE   4  (bases 1 to 3877)
  AUTHORS   Yoo,J.C., Lim,Ty., Park,J.S., Hah,Y.S., Park,N., Hong,S.G.,
            Park,J.Y. and Yoon,T.J.
  TITLE     SYT14L, especially its C2 domain, is involved in regulating
            melanocyte differentiation
  JOURNAL   J Dermatol Sci 72 (3), 246-251 (2013)
   PUBMED   23999003
REFERENCE   5  (bases 1 to 3877)
  AUTHORS   Mesentier-Louro,L.A., Coronel,J., Zaverucha-do-Valle,C.,
            Mencalha,A., Paredes,B.D., Abdelhay,E., Mendez-Otero,R. and
            Santiago,M.F.
  TITLE     Cell therapy modulates expression of Tax1-binding protein 1 and
            synaptotagmin IV in a model of optic nerve lesion
  JOURNAL   Invest Ophthalmol Vis Sci 53 (8), 4720-4729 (2012)
   PUBMED   22695963
  REMARK    GeneRIF: The increased expression of Synaptotagmin IV in optic
            nerve crush eyes displays the possible neuroprotective effect of
            bone marrow mononuclear cell treatment
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3877)
  AUTHORS   Tocco,G., Bi,X., Vician,L., Lim,I.K., Herschman,H. and Baudry,M.
  TITLE     Two synaptotagmin genes, Syt1 and Syt4, are differentially
            regulated in adult brain and during postnatal development following
            kainic acid-induced seizures
  JOURNAL   Brain Res Mol Brain Res 40 (2), 229-239 (1996)
   PUBMED   8872307
REFERENCE   7  (bases 1 to 3877)
  AUTHORS   Fukuda,M., Kojima,T. and Mikoshiba,K.
  TITLE     Phospholipid composition dependence of Ca2+-dependent phospholipid
            binding to the C2A domain of synaptotagmin IV
  JOURNAL   J Biol Chem 271 (14), 8430-8434 (1996)
   PUBMED   8626542
REFERENCE   8  (bases 1 to 3877)
  AUTHORS   Li,C., Ullrich,B., Zhang,J.Z., Anderson,R.G., Brose,N. and
            Sudhof,T.C.
  TITLE     Ca(2+)-dependent and -independent activities of neural and
            non-neural synaptotagmins
  JOURNAL   Nature 375 (6532), 594-599 (1995)
   PUBMED   7791877
REFERENCE   9  (bases 1 to 3877)
  AUTHORS   Vician,L., Lim,I.K., Ferguson,G., Tocco,G., Baudry,M. and
            Herschman,H.R.
  TITLE     Synaptotagmin IV is an immediate early gene induced by
            depolarization in PC12 cells and in brain
  JOURNAL   Proc Natl Acad Sci U S A 92 (6), 2164-2168 (1995)
   PUBMED   7892240
REFERENCE   10 (bases 1 to 3877)
  AUTHORS   Ullrich,B., Li,C., Zhang,J.Z., McMahon,H., Anderson,R.G.,
            Geppert,M. and Sudhof,T.C.
  TITLE     Functional properties of multiple synaptotagmins in brain
  JOURNAL   Neuron 13 (6), 1281-1291 (1994)
   PUBMED   7993622
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB730279.1, CB786178.1, BG666223.1, U14398.1 and JAXUCZ010000018.1.
            
            On Jan 25, 2008 this sequence version replaced NM_031693.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ211592.1, L38247.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840122, SAMN12840129
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               CB730279.1         48-359
            313-462             CB786178.1         303-452
            463-624             BG666223.1         229-390
            625-1269            U14398.1           654-1298
            1270-3877           JAXUCZ010000018.1  23311454-23314061   c
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p12"
     gene            1..3877
                     /gene="Syt4"
                     /note="synaptotagmin 4"
                     /db_xref="GeneID:64440"
                     /db_xref="RGD:68397"
     exon            1..272
                     /gene="Syt4"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..1516
                     /gene="Syt4"
                     /note="sytIV; synaptotagmin IV"
                     /codon_start=1
                     /product="synaptotagmin-4"
                     /protein_id="NP_113881.1"
                     /db_xref="GeneID:64440"
                     /db_xref="RGD:68397"
                     /translation="MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAK
                     SNKTPPYKFVHVLKGVDIYPENLSSKKKFGGDDKSEAKRKAALPNLSLHLDLEKRDLN
                     GNFPKTNPKAGSSSDLENVTPKLFPETEKEAVSPESLKSSTSLTSEEKQEKLGTLFLS
                     LEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPV
                     FDETFTFYGVPYPHIQELSLHFTVLSFDRFSRDDVIGEVLVPLSGIELSDGKMLMTRE
                     IIKRNAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHA
                     KKRISKKKTHVKKCTPNAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLV
                     LGATAEGSGGGHWKEICDFPRRQIAKWHMLCDG"
     exon            273..1087
                     /gene="Syt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1208
                     /gene="Syt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..3877
                     /gene="Syt4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcaattcgg gtttcagcca cgtctggagt ctcggagaca agcttttcag aagacccaac
       61 aggaacaggg cagggtggca cattgctgat agagagcgcg gaccttcctc cagcccctcc
      121 cgctgccgct gccggtcccc ggatcccccg tgtttcccct ggaagccttg tttcagggtc
      181 cttccaggtg aatctgcctg gagttctttc agcacctcgg acagagcacg cagaaaacat
      241 ggctcctatc accaccagcc gcgtggaatt cgatgaaatt cccacagtgg tgggcatctt
      301 cagtgctttt ggcctggtct tcactgtgtc tctctttgcc tggatctgct gccagagaag
      361 atccgctaag tccaacaaga ctcctccata caagtttgtg cacgtgctta aaggagttga
      421 tatctaccca gaaaacctaa gtagcaaaaa gaagtttgga ggagatgaca agagtgaagc
      481 aaagcgtaaa gcagctttgc ctaacctttc cctgcacctt gatctcgaga aacgagacct
      541 caatggcaat ttccccaaaa ccaaccccaa agctggcagc tcttctgatc tggaaaatgt
      601 caccccaaag ctctttccgg agacggaaaa ggaggccgtc tcccctgaga gcttgaagtc
      661 cagcacttcc ctcacttcag aggagaaaca agagaagctg ggcactctct tcttgtctct
      721 agagtacaac ttcgagaaga aagcatttgt ggtgaacatc aaggaagccc agggcttacc
      781 agccatggat gaacaatcca tgacctctga cccatacatc aagatgacaa tcttaccaga
      841 gaagaagcac aaagtgaaaa ccagagtgct gaggaagacg ctggaccccg tgtttgacga
      901 aaccttcaca ttctatgggg tcccttatcc ccacatccaa gagctgtccc tgcacttcac
      961 cgtcctgagt tttgacaggt tctcaagaga tgatgtcatt ggagaagtcc tcgttcctct
     1021 ttcagggatt gaattgtcag acgggaaaat gttaatgacc agagagatca tcaagagaaa
     1081 tgctaagaag tcttctggac ggggtgaact tctggtctcc ctctgttatc agtccactac
     1141 aaacacgctc actgtggttg tcttaaaagc gcggcaccta ccgaaatctg atgtgtctgg
     1201 actttcagat ccctacgtca aagtgaacct gtaccatgcc aagaagagaa tctctaaaaa
     1261 gaagactcat gtgaaaaagt gcactcccaa cgcagtgttc aacgaactgt ttgtctttga
     1321 tattccttgt gagagtcttg aagaaataag tgttgaattt ttagttttgg attctgaaag
     1381 gggatcccga aatgaggtga tcgggcggtt ggtcctgggt gccacagcag aaggaagtgg
     1441 tggggggcac tggaaggaga tctgtgactt ccccaggaga caaattgcta agtggcatat
     1501 gctctgtgat ggttagcttc ctggccacca gctacaactt aaaggttttt actaggcgag
     1561 aagcaatttt ctttcttata cgcgattgta agcttgtggc ccagcaagct actttactat
     1621 ttatttgttt tggttagaag tggattaaat tagtagacca gaagatagct gtaaatgtgt
     1681 attatgataa tttcctcttt tattagaggc gttggccaag ttttcataag atactgtttc
     1741 cctctgtgtg tattaagtgg gccaaccaag cctgttatga gcattctagc ttaacctcaa
     1801 gtcacagatg tgtgttgtgg gtgatttcat tttttgcctt ggtagtggaa atcaggtgat
     1861 tgttaagagt taaaagtggg aaccacacct tttttgtatg aaaattaatc catgtttctt
     1921 caggttgggg agataggttt tatttaaatc caaagatatt tccaaaatgt cctcaagtct
     1981 gttttttagt taactatgtc atgtacaaat gattgtctta tatattagtg tatgctgtca
     2041 tttccactgt aattatttga tacaatctca tcataaaagg ggtagcttag gttcatttca
     2101 taaagacagc aaagaagttg agaaattaat ttattaaagg gctgaattga agaacactgt
     2161 ggttaaaatg agattttttt tgttccatcc catgtgagtc atgtaaaatt taacttcacc
     2221 taagaaccat ggtcttgaat tattcactgc ctgtcagaag cctcagtgtg gcctaagata
     2281 ctcctactta cctttgtgaa gttgtcagac tatttagtgg gaaaaaaaaa caaacctaag
     2341 ctagaatttc agttaggaat acagttgttt tttatataaa aatctatcag tgtacaagtg
     2401 tattttcaag attgtctttc cccctcacag tggacatggc cacctaagtt ttttaactta
     2461 ttctttccca ggaagaacta agctatttaa aaaggaacga aagaaaagaa aagaaaaact
     2521 tgtgaaattc atggtcaggt tgaagtcaac cacattatgt aaaagactga gacaagaaaa
     2581 gagtaagcct ttcagaattt acaaatattc acaaaatata gttaggctgt aaaaatggca
     2641 gacagtatgt tctccaactg tctttgggga ccagttcgtt ctgagctgtt atttttaaga
     2701 tagcttaaag aatcgtgtct cgtgccactc cccaatgcta agttctgaaa ttcttgcgaa
     2761 acttgtgcaa ggcaagcaga tttctggcat ttcctgggaa gacttagttc aatggtgatg
     2821 tctttatatt tgagtcattg gagactcatg agggtgactt aggtccagcc aaattattaa
     2881 atatgcgagt tagaggtaat gtctactgga aattttacct tcgagtcaag ttttgtgtca
     2941 gtgctttggc atttttgaga atgtgtttga tatcacagtg tttgtaaatt caatgaaaat
     3001 gtatttccga accacttgtc cgtgcttttt atgactacaa caaaagtccg actttctata
     3061 aggagatata tctcattctg tttgtacaaa agactttacg ctttccccac actcatcgtc
     3121 atccagtgac aatgtcatct gtcatctctg cctactcagc aggctgagac tatcatggag
     3181 tgagctgcgg tcgtgtggag ttggggaggc cactcgcctc catatgtgtg tacatttgat
     3241 gtcataggcc atgggatgta gtgtagctcg gagctctaat gtcaagaggc accatttcca
     3301 agtatcctac ggagcggcag gaagttgatg gtgatccagt ttacaggatg tttattggaa
     3361 aaggattttg ttttttttta aaagagaaca aatctgacaa caaatacttt taaaattgta
     3421 aatagtttta cgatttaaag tgttttgacg gtgtaagtcg attgaaacta tttatggctg
     3481 gttttaaggg aaaagaagag aaccgcaaag aaaggagaaa agcaagaaaa atattgaaac
     3541 aggcagggag atatgtgatc tcattacttc tgaaaattgt actgtttata gtgtattaca
     3601 gtatcaacgt gaatatccaa ttctgctaaa tttcctgcac tgtattaaac atgtaaatta
     3661 acttttttgt ctgcttagcc agtctaacca cccggacagg gaggttgcca tgtctgaaga
     3721 actgtgtgat tcagtgatga cttgttctga cttgttgtag ttcagtagtg ttcaggagga
     3781 aagcatggtg taagggagat gctgtctgtc cctttgtgcc agcactgtat gtttgcaaga
     3841 cacgaataaa ctgccgcttt tgcccaaacg atctcaa
//
