LOCUS       NM_012665               2544 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus synaptotagmin 2 (Syt2), mRNA.
ACCESSION   NM_012665
VERSION     NM_012665.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Flores,A., Ramirez-Franco,J., Desplantes,R., Debreux,K.,
            Ferracci,G., Wernert,F., Blanchard,M.P., Maulet,Y., Youssouf,F.,
            Sangiardi,M., Iborra,C., Popoff,M.R., Seagar,M., Fantini,J.,
            Leveque,C. and El Far,O.
  TITLE     Gangliosides interact with synaptotagmin to form the high-affinity
            receptor complex for botulinum neurotoxin B
  JOURNAL   Proc Natl Acad Sci U S A 116 (36), 18098-18108 (2019)
   PUBMED   31431523
  REMARK    GeneRIF: a point mutation on synaptotagmin, located outside of the
            BoNT/B-binding segment, inhibited GT1b binding and blocked
            GT1b-induced potentiation of BoNT/B binding to
            synaptotagmin-expressing cells.
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Tejero,R., Lopez-Manzaneda,M., Arumugam,S. and Tabares,L.
  TITLE     Synaptotagmin-2, and -1, linked to neurotransmission impairment and
            vulnerability in Spinal Muscular Atrophy
  JOURNAL   Hum Mol Genet 25 (21), 4703-4716 (2016)
   PUBMED   28173138
REFERENCE   3  (bases 1 to 2544)
  AUTHORS   Wilhelm,B.G., Mandad,S., Truckenbrodt,S., Krohnert,K., Schafer,C.,
            Rammner,B., Koo,S.J., Classen,G.A., Krauss,M., Haucke,V., Urlaub,H.
            and Rizzoli,S.O.
  TITLE     Composition of isolated synaptic boutons reveals the amounts of
            vesicle trafficking proteins
  JOURNAL   Science 344 (6187), 1023-1028 (2014)
   PUBMED   24876496
REFERENCE   4  (bases 1 to 2544)
  AUTHORS   Yoo,J.C., Lim,Ty., Park,J.S., Hah,Y.S., Park,N., Hong,S.G.,
            Park,J.Y. and Yoon,T.J.
  TITLE     SYT14L, especially its C2 domain, is involved in regulating
            melanocyte differentiation
  JOURNAL   J Dermatol Sci 72 (3), 246-251 (2013)
   PUBMED   23999003
REFERENCE   5  (bases 1 to 2544)
  AUTHORS   Berntsson,R.P., Peng,L., Svensson,L.M., Dong,M. and Stenmark,P.
  TITLE     Crystal structures of botulinum neurotoxin DC in complex with its
            protein receptors synaptotagmin I and II
  JOURNAL   Structure 21 (9), 1602-1611 (2013)
   PUBMED   23932591
  REMARK    GeneRIF: Crystal structure of the rat Syt2 protein botulinum
            neurotoxin DC complex.
REFERENCE   6  (bases 1 to 2544)
  AUTHORS   Fukuda,M., Kojima,T. and Mikoshiba,K.
  TITLE     Phospholipid composition dependence of Ca2+-dependent phospholipid
            binding to the C2A domain of synaptotagmin IV
  JOURNAL   J Biol Chem 271 (14), 8430-8434 (1996)
   PUBMED   8626542
REFERENCE   7  (bases 1 to 2544)
  AUTHORS   Li,C., Ullrich,B., Zhang,J.Z., Anderson,R.G., Brose,N. and
            Sudhof,T.C.
  TITLE     Ca(2+)-dependent and -independent activities of neural and
            non-neural synaptotagmins
  JOURNAL   Nature 375 (6532), 594-599 (1995)
   PUBMED   7791877
REFERENCE   8  (bases 1 to 2544)
  AUTHORS   Ullrich,B., Li,C., Zhang,J.Z., McMahon,H., Anderson,R.G.,
            Geppert,M. and Sudhof,T.C.
  TITLE     Functional properties of multiple synaptotagmins in brain
  JOURNAL   Neuron 13 (6), 1281-1291 (1994)
   PUBMED   7993622
REFERENCE   9  (bases 1 to 2544)
  AUTHORS   Fukuda,M., Aruga,J., Niinobe,M., Aimoto,S. and Mikoshiba,K.
  TITLE     Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of
            IP4BP/synaptotagmin II
  JOURNAL   J Biol Chem 269 (46), 29206-29211 (1994)
   PUBMED   7961887
REFERENCE   10 (bases 1 to 2544)
  AUTHORS   Geppert,M., Archer,B.T. 3rd and Sudhof,T.C.
  TITLE     Synaptotagmin II. A novel differentially distributed form of
            synaptotagmin
  JOURNAL   J Biol Chem 266 (21), 13548-13552 (1991)
   PUBMED   1856191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            [WARNING] On Apr 23, 2024 this sequence was replaced by
            NM_012665.3.
            
            On Jul 13, 2017 this sequence version replaced NM_012665.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.51256.1,
                                           SRR26643298.51251.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               JAXUCZ010000013.1  48737122-48737322
            202-368             JAXUCZ010000013.1  48738479-48738645
            369-488             JAXUCZ010000013.1  48740091-48740210
            489-656             JAXUCZ010000013.1  48740510-48740677
            657-824             JAXUCZ010000013.1  48740941-48741108
            825-942             JAXUCZ010000013.1  48742266-48742383
            943-1076            JAXUCZ010000013.1  48743393-48743526
            1077-2544           JAXUCZ010000013.1  48744231-48745698
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     gene            1..2544
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /note="synaptotagmin 2"
                     /db_xref="GeneID:24805"
                     /db_xref="RGD:3804"
     exon            1..201
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1283
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /note="sytII; synaptotagmin II"
                     /codon_start=1
                     /product="synaptotagmin-2"
                     /protein_id="NP_036797.2"
                     /db_xref="GeneID:24805"
                     /db_xref="RGD:3804"
                     /translation="MRNIFKRNQEPIVAPATTTATMPLAPAAPVDNSTESTGTGESQE
                     DMFAKLKDKFFNEINKIPLPPWALIAMAVVAGLLLLTCCFCICKKCCCKKKKNKKEKG
                     KGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLT
                     VGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQ
                     ELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGD
                     ICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKK
                     TLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWS
                     DMLANPRRPIAQWHSLKPEEEVDALLGKNK"
     exon            202..368
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            369..488
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            489..656
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            657..824
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            825..942
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1076
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..2544
                     /gene="Syt2"
                     /gene_synonym="RATSYNII; SYNII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcccctctgc caccatgaga aacatcttca agaggaacca ggagcccatt gtggctccgg
       61 ccaccaccac tgccacaatg cctctggcac ccgccgcacc tgtcgataac tctacagaga
      121 gcacgggcac cggggagagc caagaagaca tgttcgccaa gctgaaggac aaattcttca
      181 atgagatcaa caagatccct ttgcccccct gggctctgat tgccatggcc gtggttgctg
      241 gcctcctgct gctcacctgc tgcttctgca tctgtaagaa gtgctgctgc aagaagaaga
      301 aaaacaagaa ggagaagggc aaaggcatga agaacgccat gaacatgaag gacatgaagg
      361 ggggccagga tgatgacgac gcggagacag gcctgactga aggagaagga gaaggcgagg
      421 aggagaaaga gccggagaac ctgggcaaat tgcagttttc tctggactat gatttccaag
      481 ccaaccagct caccgtgggc gtcctgcagg ctgctgaact cccggccctg gacatgggtg
      541 gcacgtcaga cccttatgtc aaagtcttcc tcctcccaga caagaagaag aaatatgaga
      601 ccaaggtgca ccggaagaca ctgaacccag ccttcaacga aactttcacc ttcaaggtgc
      661 cataccagga gttaggaggc aaaaccctgg tgatggctat ctatgacttt gaccgcttct
      721 ctaagcatga catcatcggg gaggtgaaag tgcccatgaa cacggtggac cttggccagc
      781 ccatcgagga atggagagac ctacaaggcg gagagaagga agagccagag aagctgggtg
      841 acatctgtac ctccttgcgc tacgtgccca ctgctgggaa gctcaccgtc tgtatcctgg
      901 aggccaagaa cctgaagaag atggatgtgg ggggcctctc agacccctat gtgaagatcc
      961 acttgatgca gaatggcaag agactcaaga agaagaagac gacggtgaag aagaagacct
     1021 tgaaccccta cttcaatgag tcattcagct tcgagatccc ctttgagcag atccagaaag
     1081 tccaggtggt cgtcaccgtg ctagactatg acaaactggg caagaatgaa gccattggaa
     1141 agatcttcgt aggcagcaac gctacaggca cggagctgcg gcactggtcc gacatgctgg
     1201 cgaaccctcg gaggcccatc gcccagtggc actctctgaa gcctgaggaa gaagtggatg
     1261 ctcttctggg caagaacaag taggcagcgg cggctggggc cacgccccag aggacactga
     1321 cgagctccag agctatcaat acctcagtta tgcgacctta gaggtttctt catttgtttg
     1381 cggtgtgtcc tgttttcctt tcctttttct ttttttgtct ttttaaaaac caacttcctt
     1441 ttggtggcta tgtgaagagt cccctaagac gtgaaagaga agcctggctc tgttattgtc
     1501 ccaggagctg tccttgttgc atgccctatc acggtttccc ctcaccccaa gtggggccct
     1561 ctactgtcag agtggaagca cttcctgctt ttcctgggtt ttggaccaac aaagtggcaa
     1621 gcacattctg tgtctcgact gtgtgtgtgt gtgtgtgtgt gtacacgtgt gcccatccat
     1681 ccccaccttg cctctgtttg gaatatctct tcgtttctgg aatgagtcat ggacaatgat
     1741 gccgtgtgag agaggaaagt cttcaggtac tccgaggtga ggagagccca ctgcttaagt
     1801 ggtcagaggc cagaagctct catagtcctt gcgaaaggcc atttggaaga cgcaagatgt
     1861 gatactggat gtgttccgaa ctaggaccaa aggcttgatg ccatcccaga ctccctcttg
     1921 tcagtcatgg cttccccagg agtggggctt tgggatcatt catgaaaata aactatttac
     1981 tcgactggtc ggattcagcc agggaccgcc agctccagga tgtcattctt gttgacgaca
     2041 tcaaactttg aagaaacaga agtcccatta ctcagctctg gatctttgcc tcgtccagtg
     2101 ggaggcagat gcttcccccc tctgcagagt acaagcagtg cgttcatttg cattcacgca
     2161 ccatctgctt ttgcctctgt ttcccttttt gtgtaagtgg aaaaatacca tctgacgata
     2221 agtgctttgc acagagccag agacctatta gagggacgct tgggtgttta gttcccttga
     2281 ggtccaggta aggaggaggt gtcaagaagg ggagcgttgg tggacagtga caagctagac
     2341 attgcagagc tcctcacaac tcctattcct gaccctctgg accctttgac cctcagtgat
     2401 ggtagccgga gtagcccagg cagaccttag gagaggcccc gtccttccct tccttagaca
     2461 gttttctcag aatgccagga aacacagcgc attcatttca gatgggtggt ggagaaaatg
     2521 tgctaaggtt tgcaccctat gttc
//
