LOCUS       NM_001033680            1896 bp    mRNA    linear   ROD 20-AUG-2024
DEFINITION  Rattus norvegicus synaptotagmin 1 (Syt1), mRNA.
ACCESSION   NM_001033680 XM_343205
VERSION     NM_001033680.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Mehta,N., Mondal,S., Watson,E.T., Cui,Q. and Chapman,E.R.
  TITLE     The juxtamembrane linker of synaptotagmin 1 regulates Ca2+ binding
            via liquid-liquid phase separation
  JOURNAL   Nat Commun 15 (1), 262 (2024)
   PUBMED   38177243
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Chen,Y., Hu,S., Wu,X., Xie,Z., Wang,Y., Wang,B., Li,X., Pei,Y.,
            Gu,Y., Huang,K., Huo,J., Wei,A., Bi,C., Lu,Z., Song,Q., Xu,H.,
            Kang,X., Shao,S., Long,J., Liu,J., Zhou,Z., Huang,R., Chai,Z. and
            Wang,C.
  TITLE     Synaptotagmin-1 is a bidirectional Ca2+ sensor for neuronal
            endocytosis
  JOURNAL   Proc Natl Acad Sci U S A 119 (20), e2111051119 (2022)
   PUBMED   35537054
  REMARK    GeneRIF: Synaptotagmin-1 is a bidirectional Ca(2+) sensor for
            neuronal endocytosis.
REFERENCE   3  (bases 1 to 1896)
  AUTHORS   Barbier,E., Barchiesi,R., Domi,A., Chanthongdee,K., Domi,E.,
            Augier,G., Augier,E., Xu,L., Adermark,L. and Heilig,M.
  TITLE     Downregulation of Synaptotagmin 1 in the Prelimbic Cortex Drives
            Alcohol-Associated Behaviors in Rats
  JOURNAL   Biol Psychiatry 89 (4), 398-406 (2021)
   PUBMED   33160605
  REMARK    GeneRIF: Downregulation of Synaptotagmin 1 in the Prelimbic Cortex
            Drives Alcohol-Associated Behaviors in Rats.
REFERENCE   4  (bases 1 to 1896)
  AUTHORS   Nyenhuis,S.B., Karandikar,N., Kiessling,V., Kreutzberger,A.J.B.,
            Thapa,A., Liang,B., Tamm,L.K. and Cafiso,D.S.
  TITLE     Conserved arginine residues in synaptotagmin 1 regulate fusion pore
            expansion through membrane contact
  JOURNAL   Nat Commun 12 (1), 761 (2021)
   PUBMED   33536412
  REMARK    GeneRIF: Conserved arginine residues in synaptotagmin 1 regulate
            fusion pore expansion through membrane contact.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1896)
  AUTHORS   Stepien,K.P. and Rizo,J.
  TITLE     Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion
            with a few neuronal SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   33468652
  REMARK    GeneRIF: Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent
            liposome fusion with a few neuronal SNAREs.
REFERENCE   6  (bases 1 to 1896)
  AUTHORS   McMahon,H.T., Missler,M., Li,C. and Sudhof,T.C.
  TITLE     Complexins: cytosolic proteins that regulate SNAP receptor function
  JOURNAL   Cell 83 (1), 111-119 (1995)
   PUBMED   7553862
REFERENCE   7  (bases 1 to 1896)
  AUTHORS   Li,C., Ullrich,B., Zhang,J.Z., Anderson,R.G., Brose,N. and
            Sudhof,T.C.
  TITLE     Ca(2+)-dependent and -independent activities of neural and
            non-neural synaptotagmins
  JOURNAL   Nature 375 (6532), 594-599 (1995)
   PUBMED   7791877
REFERENCE   8  (bases 1 to 1896)
  AUTHORS   Sutton,R.B., Davletov,B.A., Berghuis,A.M., Sudhof,T.C. and
            Sprang,S.R.
  TITLE     Structure of the first C2 domain of synaptotagmin I: a novel
            Ca2+/phospholipid-binding fold
  JOURNAL   Cell 80 (6), 929-938 (1995)
   PUBMED   7697723
REFERENCE   9  (bases 1 to 1896)
  AUTHORS   Perin,M.S., Fried,V.A., Mignery,G.A., Jahn,R. and Sudhof,T.C.
  TITLE     Phospholipid binding by a synaptic vesicle protein homologous to
            the regulatory region of protein kinase C
  JOURNAL   Nature 345 (6272), 260-263 (1990)
   PUBMED   2333096
REFERENCE   10 (bases 1 to 1896)
  AUTHORS   Matthew,W.D., Tsavaler,L. and Reichardt,L.F.
  TITLE     Identification of a synaptic vesicle-specific membrane protein with
            a wide distribution in neuronal and neurosecretory tissue
  JOURNAL   J Cell Biol 91 (1), 257-269 (1981)
   PUBMED   7298720
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AJ617621.1, AA924659.1, CB731032.1, CO398869.1 and CB615786.1.
            
            On May 26, 2007 this sequence version replaced NM_001033680.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-682               AJ617621.1         1-682
            683-911             AA924659.1         11-239              c
            912-983             CB731032.1         285-356
            984-1592            CO398869.1         44-652
            1593-1896           CB615786.1         267-570
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q21"
     gene            1..1896
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /note="synaptotagmin 1"
                     /db_xref="GeneID:25716"
                     /db_xref="RGD:3803"
     exon            1..162
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            163..263
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            264..325
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            326..505
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     CDS             343..1608
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /note="sytI; synaptotagmin I"
                     /codon_start=1
                     /product="synaptotagmin-1"
                     /protein_id="NP_001028852.2"
                     /db_xref="GeneID:25716"
                     /db_xref="RGD:3803"
                     /translation="MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQ
                     KFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAIN
                     MKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLV
                     GIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSE
                     LGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDI
                     CFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNT
                     LNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSD
                     MLANPRRPIAQWHTLQVEEEVDAMLAVKK"
     exon            506..690
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            691..813
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            814..981
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1149
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1267
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1401
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1896
                     /gene="Syt1"
                     /gene_synonym="P65"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctggtggccc tagaaaacct cacccacacc cacacacccc ttttgtgttg caggctgccc
       61 ctctgagagc ggaggcagcg agagtactcg cgtgcctcgc accggtccgc ggtgagagct
      121 gcggggacca agactcgcac cacctcccgg tcctcgctcc aggaaaagaa gacttgaaag
      181 cgcttgagca accaacatcc gcagtcagat cggaagactt tgccctggcc atccccagag
      241 cgccaccaga acgtctcatt aagattgaag aaagattccg agaagaacaa aaccccccaa
      301 atactccata ataccctgca gaacatttca cttgaaccaa aaatggtgag tgccagtcat
      361 cctgaggccc tggccgcccc tgtcaccact gttgcgaccc ttgtcccaca caatgccact
      421 gagccagcca gtcctgggga agggaaggaa gatgcctttt ccaagctgaa gcagaagttt
      481 atgaatgagc tgcataaaat tccattgcca ccgtgggcct taatagccat agccatagtt
      541 gcggtccttt tagtcgtaac ctgctgcttt tgtgtctgta agaaatgttt gttcaaaaag
      601 aaaaacaaga agaaggggaa ggaaaaggga ggaaagaacg ccattaacat gaaagacgtg
      661 aaagacttag ggaagaccat gaaggatcag gcccttaagg atgacgatgc tgaaaccgga
      721 ctgactgatg gagaagaaaa ggaagagccc aaggaagagg agaaactggg aaagctccaa
      781 tattcactgg actatgactt ccagaataac cagctgttgg tgggaatcat ccaggctgct
      841 gaactgcccg ccctggacat ggggggtaca tccgatccat acgtcaaagt cttcctgctg
      901 cctgacaaaa agaagaaatt tgagactaaa gtccaccgga aaaccctcaa tccagtcttc
      961 aatgaacaat ttactttcaa ggtgccctac tcggaattag gtggcaaaac cctggtgatg
     1021 gctgtgtatg actttgatcg cttctccaag catgacatca tcggagagtt caaagttcct
     1081 atgaacaccg tggattttgg ccatgtgacc gaggagtggc gtgatctcca gagcgctgag
     1141 aaagaagagc aagagaaact gggtgacatc tgcttctccc tccgctacgt ccctactgcc
     1201 ggcaaactga ctgttgtcat tctggaagcc aagaacctga agaagatgga tgtgggtggc
     1261 ttatctgatc cctacgtgaa gattcacctg atgcagaacg gtaagaggct gaagaagaaa
     1321 aagacgacga ttaagaagaa cacactcaac ccctactaca acgagtcctt cagctttgaa
     1381 gttccgttcg agcaaatcca gaaagtgcaa gtggtggtaa ctgttttgga ctatgacaag
     1441 attggcaaga acgacgccat cggcaaagtc ttcgttggtt acaacagcac tggggcggag
     1501 ctgcgacact ggtcagacat gctggccaac ccccggcgac ccatcgcaca gtggcacact
     1561 ctgcaggtag aggaggaggt tgatgccatg ctggctgtca agaagtaaag ggaaaacgaa
     1621 gcctttctgc atctgcccac atagtgctct ttagccagta tctgtaaata cctcagtaat
     1681 atgggtcctt ttggtttcca gccatgcatt cctgatacaa tccagtggta cttcaaatcc
     1741 tgttttaatt tgcacaaatt taagtgtaga aagcccttat gccctccatc ataccactgc
     1801 cctccaaatc tactcttctt ttaagcaata tgatgtgtag atagagcatg actgaaatta
     1861 tgtattgtat cacactgttg tatataccag tatgct
//
