LOCUS       NM_053476               7147 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus synaptojanin 1 (Synj1), transcript variant 1,
            mRNA.
ACCESSION   NM_053476 XM_001073290 XM_573256
VERSION     NM_053476.4
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Hunn,B.H., Cragg,S.J., Bolam,J.P., Spillantini,M.G. and
            Wade-Martins,R.
  TITLE     Impaired intracellular trafficking defines early Parkinson's
            disease
  JOURNAL   Trends Neurosci 38 (3), 178-188 (2015)
   PUBMED   25639775
  REMARK    Review article
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Drouet,V. and Lesage,S.
  TITLE     Synaptojanin 1 mutation in Parkinson's disease brings further
            insight into the neuropathological mechanisms
  JOURNAL   Biomed Res Int 2014, 289728 (2014)
   PUBMED   25302295
  REMARK    Review article
REFERENCE   3  (bases 1 to 7147)
  AUTHORS   Saheki,Y. and De Camilli,P.
  TITLE     Synaptic vesicle endocytosis
  JOURNAL   Cold Spring Harb Perspect Biol 4 (9), a005645 (2012)
   PUBMED   22763746
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7147)
  AUTHORS   Cossec,J.C., Lavaur,J., Berman,D.E., Rivals,I., Hoischen,A.,
            Stora,S., Ripoll,C., Mircher,C., Grattau,Y., Olivomarin,J.C., de
            Chaumont,F., Lecourtois,M., Antonarakis,S.E., Veltman,J.A.,
            Delabar,J.M., Duyckaerts,C., Di Paolo,G. and Potier,M.C.
  TITLE     Trisomy for synaptojanin1 in Down syndrome is functionally linked
            to the enlargement of early endosomes
  JOURNAL   Hum Mol Genet 21 (14), 3156-3172 (2012)
   PUBMED   22511594
REFERENCE   5  (bases 1 to 7147)
  AUTHORS   Milosevic,I., Giovedi,S., Lou,X., Raimondi,A., Collesi,C., Shen,H.,
            Paradise,S., O'Toole,E., Ferguson,S., Cremona,O. and De Camilli,P.
  TITLE     Recruitment of endophilin to clathrin-coated pit necks is required
            for efficient vesicle uncoating after fission
  JOURNAL   Neuron 72 (4), 587-601 (2011)
   PUBMED   22099461
REFERENCE   6  (bases 1 to 7147)
  AUTHORS   Ringstad,N., Nemoto,Y. and De Camilli,P.
  TITLE     The SH3p4/Sh3p8/SH3p13 protein family: binding partners for
            synaptojanin and dynamin via a Grb2-like Src homology 3 domain
  JOURNAL   Proc Natl Acad Sci U S A 94 (16), 8569-8574 (1997)
   PUBMED   9238017
REFERENCE   7  (bases 1 to 7147)
  AUTHORS   de Heuvel,E., Bell,A.W., Ramjaun,A.R., Wong,K., Sossin,W.S. and
            McPherson,P.S.
  TITLE     Identification of the major synaptojanin-binding proteins in brain
  JOURNAL   J Biol Chem 272 (13), 8710-8716 (1997)
   PUBMED   9079704
REFERENCE   8  (bases 1 to 7147)
  AUTHORS   Ramjaun,A.R. and McPherson,P.S.
  TITLE     Tissue-specific alternative splicing generates two synaptojanin
            isoforms with differential membrane binding properties
  JOURNAL   J Biol Chem 271 (40), 24856-24861 (1996)
   PUBMED   8798761
REFERENCE   9  (bases 1 to 7147)
  AUTHORS   McPherson,P.S., Garcia,E.P., Slepnev,V.I., David,C., Zhang,X.,
            Grabs,D., Sossin,W.S., Bauerfeind,R., Nemoto,Y. and De Camilli,P.
  TITLE     A presynaptic inositol-5-phosphatase
  JOURNAL   Nature 379 (6563), 353-357 (1996)
   PUBMED   8552192
REFERENCE   10 (bases 1 to 7147)
  AUTHORS   McPherson,P.S., Takei,K., Schmid,S.L. and De Camilli,P.
  TITLE     p145, a major Grb2-binding protein in brain, is co-localized with
            dynamin in nerve terminals where it undergoes activity-dependent
            dephosphorylation
  JOURNAL   J Biol Chem 269 (48), 30132-30139 (1994)
   PUBMED   7982917
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Dec 20, 2022 this sequence version replaced NM_053476.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U45479.2, SRR26360176.709055.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               JAXUCZ010000011.1  43755201-43755526   c
            327-472             JAXUCZ010000011.1  43754153-43754298   c
            473-559             JAXUCZ010000011.1  43737853-43737939   c
            560-827             JAXUCZ010000011.1  43734681-43734948   c
            828-1053            JAXUCZ010000011.1  43731536-43731761   c
            1054-1137           JAXUCZ010000011.1  43730764-43730847   c
            1138-1199           JAXUCZ010000011.1  43726106-43726167   c
            1200-1296           JAXUCZ010000011.1  43725293-43725389   c
            1297-1466           JAXUCZ010000011.1  43724224-43724393   c
            1467-1548           JAXUCZ010000011.1  43723044-43723125   c
            1549-1701           JAXUCZ010000011.1  43720892-43721044   c
            1702-1858           JAXUCZ010000011.1  43718499-43718655   c
            1859-1882           JAXUCZ010000011.1  43716214-43716237   c
            1883-2074           JAXUCZ010000011.1  43713810-43714001   c
            2075-2159           JAXUCZ010000011.1  43712047-43712131   c
            2160-2300           JAXUCZ010000011.1  43708622-43708762   c
            2301-2493           JAXUCZ010000011.1  43708337-43708529   c
            2494-2652           JAXUCZ010000011.1  43707941-43708099   c
            2653-2809           JAXUCZ010000011.1  43705478-43705634   c
            2810-2927           JAXUCZ010000011.1  43704862-43704979   c
            2928-3143           JAXUCZ010000011.1  43704546-43704761   c
            3144-3222           JAXUCZ010000011.1  43702764-43702842   c
            3223-3385           JAXUCZ010000011.1  43699591-43699753   c
            3386-3595           JAXUCZ010000011.1  43693654-43693863   c
            3596-3730           JAXUCZ010000011.1  43692243-43692377   c
            3731-3769           JAXUCZ010000011.1  43691598-43691636   c
            3770-3808           JAXUCZ010000011.1  43689432-43689470   c
            3809-3879           JAXUCZ010000011.1  43689209-43689279   c
            3880-3988           JAXUCZ010000011.1  43687194-43687302   c
            3989-4206           JAXUCZ010000011.1  43686757-43686974   c
            4207-4233           JAXUCZ010000011.1  43683061-43683087   c
            4234-7147           JAXUCZ010000011.1  43678709-43681622   c
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q11"
     gene            1..7147
                     /gene="Synj1"
                     /note="synaptojanin 1"
                     /db_xref="GeneID:85238"
                     /db_xref="RGD:69434"
     exon            1..326
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     CDS             229..4227
                     /gene="Synj1"
                     /EC_number="3.1.3.36"
                     /note="isoform 1 is encoded by transcript variant 1;
                     synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1"
                     /codon_start=1
                     /product="synaptojanin-1 isoform 1"
                     /protein_id="NP_445928.3"
                     /db_xref="GeneID:85238"
                     /db_xref="RGD:69434"
                     /translation="MRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFS
                     KGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGL
                     LGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVR
                     KVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDW
                     LLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQV
                     IYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYG
                     KQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSVLK
                     PQVQKFLDYGFFYFDGSAVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALG
                     LAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFF
                     DSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYK
                     YSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDI
                     FAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFI
                     RPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKER
                     NEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGD
                     QLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWP
                     FDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVE
                     AEERQKIYKEVIAVQGPPDGTVLVSIKSSAQENTFFDDALIDELLQQFAHFGEVILIR
                     FVEDKMWVTFLEGSSALNVLSLNGKELLNRTITITLKSPDWIKTLEEEMSLEKISVTL
                     PSSTSSTLLGEDAEVSADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRT
                     SPCQSPTAPEYSAPSLPIRPSRAPSRTPGPLSSQGAPVDTQPAAQKESSQTIEPKRPP
                     PPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGL
                     AGPGPSGYGAARPTIPARAGVISAPQSQARVSAGRLTPESQSKPLETSKGPAVLPEPL
                     KPQAAFPPQPSLPTPAQKLQDPLVPIAAPMPPSIPQSNLETPPLPPPRSRSSQSLPSD
                     SSPQLQQEQPTG"
     exon            327..472
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..559
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            560..827
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1053
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1137
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1199
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1296
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1466
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1548
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1701
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1858
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1882
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2074
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2159
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2300
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2493
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2652
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2809
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2927
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..3143
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3144..3222
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3223..3385
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..3595
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3596..3730
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3769
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3770..3808
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3809..3879
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3880..3988
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            3989..4206
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            4207..4233
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
     exon            4234..7147
                     /gene="Synj1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagctagagg gctccacgcg aggaggccgg accctccctc tgccggctcc cctggccgtg
       61 cctcccgtcc actcccgggg tgaccgaggc ttcacggacc ctggtcgcat cggccgcaaa
      121 aactaggtgg ccagcaaacg cccggctccg ctgcacggtc ccgcgtgggc ggaaacggct
      181 ccgccccttc accccaaggg gcgggttttc cgccccggca gcggcgcaat gcggaagagc
      241 tggacctgct ggagcggaag tgacgcttcg ggcggctgta gcggcggcgg ctgcgggaga
      301 aggagaagga ggagccggag gaagagggct gcctctgaag aaaggagaat ggcgttcagc
      361 aaaggatttc gaatctacca caaattggat cccccaccgt tcagcctcat agtggaaact
      421 aggcataagg aagaatgtct catgttcgag tctggggccg tggcagtgct ctcatctgca
      481 gaaaaggagg ccattaaagg cacatatgcc aaagtgctgg atgcatatgg acttttggga
      541 gttttacgat taaatcttgg tgataccatg ttgcattatc tggtcctagt cactggatgc
      601 atgtctgttg ggaaaattca agaatctgaa gtcttccgag ttacttccac tgagtttata
      661 tcacttcgag ttgatgcttc agatgaggac cgcatttcag aagtacgaaa ggttttgaac
      721 tcaggaaact tttattttgc atggtctgca tctggagtca gcttagatct gagtctgaac
      781 gcacatcgaa gcatgcagga gcacacaact gacaataggt ttttctggaa tcagtctttg
      841 catctgcatc tcaagcacta tggtgtgaat tgtgatgact ggttgttacg gctcatgtgt
      901 ggaggagtag agattagaac aatttatgct gcccataaac aggcaaaagc ttgcctcatt
      961 tcaagactaa gctgtgagcg agctgggaca aggttcaacg tccggggaac aaatgatgac
     1021 ggtcatgttg ccaactttgt agagacagag caggttatat acttagatga ctgtgtctcc
     1081 tccttcatac aaatccgagg atctgttccg ttgttctggg agcaaccagg gttgcaagtg
     1141 ggatctcatc gtgttcgaat gtcaagggga tttgaagcca atgcgcctgc ctttgacagg
     1201 catttccgaa cccttaagga cttatatggt aaacaaatag tagtaaatct gcttggatct
     1261 aaggagggtg agcatatgct gagcaaggcc ttccagagtc acttgaaagc ctctgaacac
     1321 gcttctgata tccacatggt gtcctttgat tatcatcaaa tggttaaagg aggaaaggca
     1381 gaaaaattgc acagtgttct caaaccacaa gtccagaagt ttctagatta tggatttttt
     1441 tatttcgatg gcagtgcagt tcaaaggtgc cagagtggta cagttcggac aaactgcctg
     1501 gactgtctgg atagaacaaa cagtgtgcag gcgttccttg gcttagagat gctagctaaa
     1561 cagctggaag ctctgggctt ggctgaaaag cctcagttgg tgactcgctt tcaagaagtt
     1621 ttccggtcca tgtggtctgt gaatggcgat tcaatcagta aaatatatgc aggcaccgga
     1681 gccctggaag ggaaggccaa gttaaaagat ggtgcgcgat ctgtcaccag aaccattcag
     1741 aataacttct tcgacagctc caagcaagaa gccattgatg ttctgcttct gggaaacact
     1801 ctgaacagcg acttagccga caaagcccgg gcacttctaa ctactgggag tttgcgtgtg
     1861 tctgaacaga cattacagtc agcatcttcc aaagtcttaa agaacatgtg tgagaacttc
     1921 tacaagtact caaagcccaa gaagatccga gtgtgtgttg gaacctggaa tgtgaacggc
     1981 gggaaacagt tccgcagcat agcattcaag aaccagaccc tcaccgactg gcttcttgat
     2041 gcacccaagt tagctggcat ccaggagttt caagataaga gaagtaagcc aactgatata
     2101 tttgcaattg gctttgaaga aatggtggag ctgaatgctg ggaacattgt gaatgcaagc
     2161 acaacaaacc agaagctgtg ggcagtggaa ctgcagaaga ccatctccag agacaacaag
     2221 tatgttctcc tggcctcgga gcagttggtg ggtgtctgcc tgtttgtctt tatcagaccg
     2281 cagcatgctc cctttatcag ggatgttgca gtcgatactg tgaaaactgg gatgggaggc
     2341 gcgactggaa ataagggcgc tgttgcaatc cggatgctct tccacaccac cagcctttgc
     2401 tttgtctgca gtcactttgc tgcaggccag tcccaagtca aagagagaaa tgaagatttc
     2461 gtagaaatag cacgaaaatt gagtttcccc atgggaagaa tgctcttctc ccatgattac
     2521 gtattctggt gtggtgattt caactatcga atcgatcttc ctaatgaaga agttaaagag
     2581 ctcataagac agcaaaattg ggattctctt atagctggag atcaactcat caatcagaaa
     2641 aatgcaggac agatctttag aggattttta gagggaaaag taacgtttgc tccaacctat
     2701 aaatatgact tgttttccga agactatgac accagtgaaa agtgccgcac ccctgcttgg
     2761 acagaccgag tcctctggag aaggaggaag tggccttttg acagatcagc tgaagactta
     2821 gatcttctga atgctagttt ccaagatgaa agtaaaatcc tgtatacatg gactcctggc
     2881 accttgctgc actatggaag agccgagctg aagacttctg accacaggcc cgttgtcgcc
     2941 ctgatcgata tagatatatt tgaagttgaa gctgaagaga gacaaaaaat ttataaagaa
     3001 gttattgcag tccagggccc accggacggc acagtactgg tctcaatcaa aagttctgca
     3061 caagagaata ctttctttga cgatgctttg atcgatgagc ttctgcagca gtttgcacac
     3121 ttcggcgaag ttatacttat aagattcgta gaagataaaa tgtgggttac atttttggag
     3181 gggagctctg ccttgaatgt tctgagccta aatggtaaag agttattaaa tcggactata
     3241 acaattactt taaaaagtcc agactggatc aaaactttgg aagaagagat gagtttagag
     3301 aaaatcagtg tcacattgcc ctcatcaacc agttccaccc tgctcggtga agatgcagag
     3361 gtctcagcgg actttgacat ggaaggtgat gtcgacgact acagtgctga ggtggaggag
     3421 cttcttcctc agcacctcca gccatcctcc agctctggcc tcggcacctc tccaagctct
     3481 tcgccccgga ccagtccctg ccagtcaccc acagcaccag agtactccgc accttctctt
     3541 cccatcagac ctagtcgagc accatcaaga actccaggac ctctaagttc acagggtgct
     3601 ccagttgaca cccagccagc agcccagaag gagtcttccc agaccataga gcccaagagg
     3661 ccaccccctc cccgcccagt tgctcctcca gcacgtcctg ccccaccaca gaggccacct
     3721 ccaccttcag gggctaggag tccggcacct gctagaaaag aatttggagc acccaaaagc
     3781 ccgggaacag cacggaaaga taatataggg cgtaatcagc cttcccctca agccggactt
     3841 gcaggcccag gaccttctgg atatggtgca gctagaccga caattccagc tcgtgctgga
     3901 gtgatcagtg cccctcagag ccaggctcgg gtgtctgctg gaaggctgac tcctgaaagc
     3961 caaagcaaac ccttggagac atcgaaaggt cctgctgtcc ttccagaacc actgaagcct
     4021 caggctgcat ttcctccgca gccttctctg cccacacctg ctcaaaagtt acaggaccca
     4081 cttgtcccca tagcagcacc tatgcctccc tccattcccc aatcgaattt ggaaactcca
     4141 cccctgcccc cacctcggag caggtcatct caaagcttgc cttcagactc ctcaccacag
     4201 ctacaacagg agcaaccaac agggtaacag caggtaaaaa taaatggagc ttgtggtgtc
     4261 aaacaagagc caacattgaa gagtgaccca tttgaagacc tctcacttag tgtgcttgct
     4321 gtatccaagg ctcagccttc tgctcagatt tcacctgttc taaccccaga cccaaagatg
     4381 ttgattcagt tgccttctgc atcgcaaagt aaagttaact ctttgagttc tgtaagttgc
     4441 atgctaacca tgcctccagt tccagagcaa agcaagtcac aggaaagtgt ggggagttct
     4501 gcaaacccat ttcccagcct gcctaccagg aaccctttta cagacaggac tgctgcccct
     4561 ggaaacccat ttagagttca gtctcaagaa tcagaggcaa cttcttggct atccaaagaa
     4621 gagcctgttt ctaacagtcc tttccctcct ctcatgcctc tcagtcacga catgagcaag
     4681 ccttcaagtt cactagatgg ttttgaggac aattttgatc tgcagagcca gtctacagta
     4741 aaaacaagca accccaaagg atgggtaaca tttgacgaag atgaagactt tcctacaaaa
     4801 ggaaagtcaa ggtcggttta tccagactcc ctgggtaata cagcagcttc atttgatgat
     4861 gactggagta aaggtacaaa cgtttctttc tgtgtgctgc cagccagaag gccaccacct
     4921 ccaccacctc ctgtccctct gctaccacct ggcaccacct catctgcagg acctagcaca
     4981 accctgtcat ctaaggcatc tcccacattg gacttcacag aaagataatg acatggaggg
     5041 aagggagttt ctcctggtgt ggtgtgactg tttgtttctg tttgtcaggg tagacccaag
     5101 agaatggggc attaattagt tgttatgtgc aataaataat cagtaagtgc actgatatct
     5161 atataaaata ccactagagg atgtggacaa agctggagtt tgtgtatatc agaaatagca
     5221 aagacatcct cttaactgga gttctggcct ttctcttcag atgaatggaa gagaatagga
     5281 accattttta aaagtgctta aaactactct agaatagtcc ttactcagat actcacatag
     5341 caatcacgta aagcccacac aactgcagct gacagtactg ccagcccatt gtgctgttga
     5401 cgattggtat tcgtggatac ttaccaaaga gcttccagag ttctggtgag attgctttga
     5461 acggcattac tttagccaaa aggtctgtgt atatatacac tgtgtgtgta tgtctgtatg
     5521 tatgcacaaa cacacatgtg tacatccaca tacttataaa atctgtcttg atataattca
     5581 gatcaaattt tacctaacag ttctgacaat gtcctttttt tcaatatctc actatcaaga
     5641 agctggctgc cactgggtgt gcttcattac gtcactaact gcctcccaga atggaagggt
     5701 cagtcccttt acagagctgc catctcctgt tacgctgcac ctcagacctt cacattcatc
     5761 ctactacctt cctcctctgg tatgcctttc cctgggcatt tacaatgggc agtacagacg
     5821 tggtcagtca tgtgacagat actcaaaaca gtaggcacct gccaccagat tccaggagga
     5881 acactatcag tgtgctcctt tctctgcaca gccctttccc ttcagacctt tctgtttgcc
     5941 aattgcctgg agctttgctc tcagctgttg agtggtccga attaggaaca tgtccagtat
     6001 aggttctctc ccatgtaaga ggctacataa catatccatg atgctgcttt aaggttttag
     6061 aggctatacc tcaaattagc tgtggatttt gtctcctgca ctgccagtat gcaactgatc
     6121 ttgcatttat taattttgtg aaaaaataca tggaattttt acaaaaaaaa aaaaacaaaa
     6181 aacaaaaaac aaaaacaccc aaaaccatag tattttgata tcattaggta agccagtcag
     6241 aaaattccct aagctattgt tttgttggtt gtcagttgaa gttaaaatca tccttaccca
     6301 ttaagactta atgttctctt tatataaaaa gagttccatg cttacaccca agttcctagt
     6361 ctacatgaac ctttaaagat gtgtattgtg agattagaaa gaatttatgg ctgccctcac
     6421 ctctgtaaaa gcacagcaga ctgcattgca cttctatcat agagcagatc tgttaggaca
     6481 gagcaccttt gcaaatgctc agtgttatca aaccctgtat ttatagaagt aactctctgc
     6541 tacagccgta acttgctgtt agcaataact cttgtgagtt ttatattgtg cttgttgggg
     6601 ttctaaacat ttcagcagta gtagctttta aactgcagta ttactagagc agtgtacttc
     6661 aaaatgtgaa ttaatttgtg gagattgtgg ctgtaacatc tgtgtgagta gtgtgtatgg
     6721 tatccactcc ccattagcat aatccatttt aggaaactga agcagagtgt ggattgcagc
     6781 cccgagcagt gactccctcg ggtataaacc actgtaatgt ctgcagagca atagctcttc
     6841 cttatcagga agtctggtag ctgctgaaac gcacaaagga agttaaagtt tgctacaaag
     6901 aactcaacat tgtgtgtatt taccagctct ggtcaaagca gaaaacagac actaagaatc
     6961 tttgtgagtc tctgttctct ctagtagatt gctgtttata tgtatgtaag aggtttattg
     7021 cttatgtggc atacagtatt tataactata tactttatag aagtacagta ttaaagtcgc
     7081 tggtatacat tctgtacata tcctgtgaaa tgtgctgtca tatgaaataa atatgtcttt
     7141 accacta
//
