LOCUS       NM_181092               5868 bp    mRNA    linear   ROD 09-OCT-2024
DEFINITION  Rattus norvegicus synaptic Ras GTPase activating protein 1
            (Syngap1), transcript variant 1, mRNA.
ACCESSION   NM_181092
VERSION     NM_181092.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Katsanevaki,D., Till,S.M., Buller-Peralta,I., Nawaz,M.S.,
            Louros,S.R., Kapgal,V., Tiwari,S., Walsh,D., Anstey,N.J.,
            Petrovic,N.G., Cormack,A., Salazar-Sanchez,V., Harris,A.,
            Farnworth-Rowson,W., Sutherland,A., Watson,T.C., Dimitrov,S.,
            Jackson,A.D., Arkell,D., Biswal,S., Dissanayake,K.N., Mizen,L.A.M.,
            Perentos,N., Jones,M.W., Cousin,M.A., Booker,S.A., Osterweil,E.K.,
            Chattarji,S., Wyllie,D.J.A., Gonzalez-Sulser,A., Hardt,O.,
            Wood,E.R. and Kind,P.C.
  TITLE     Key roles of C2/GAP domains in SYNGAP1-related pathophysiology
  JOURNAL   Cell Rep 43 (9), 114733 (2024)
   PUBMED   39269903
  REMARK    GeneRIF: Key roles of C2/GAP domains in SYNGAP1-related
            pathophysiology.
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Qvist,J.S., Scherma,M., Jayaram-Lindstrom,N., Fratta,W.,
            Kandel,D.B., Kandel,E.R., Fadda,P. and Melas,P.A.
  TITLE     Synaptoproteomic Analysis of the Prefrontal Cortex Reveals
            Spatio-Temporal Changes in SYNGAP1 Following Cannabinoid Exposure
            in Rat Adolescence
  JOURNAL   Int J Mol Sci 24 (1), 698 (2022)
   PUBMED   36614142
  REMARK    GeneRIF: Synaptoproteomic Analysis of the Prefrontal Cortex Reveals
            Spatio-Temporal Changes in SYNGAP1 Following Cannabinoid Exposure
            in Rat Adolescence.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5868)
  AUTHORS   Zeng,M., Shang,Y., Araki,Y., Guo,T., Huganir,R.L. and Zhang,M.
  TITLE     Phase Transition in Postsynaptic Densities Underlies Formation of
            Synaptic Complexes and Synaptic Plasticity
  JOURNAL   Cell 166 (5), 1163-1175 (2016)
   PUBMED   27565345
REFERENCE   4  (bases 1 to 5868)
  AUTHORS   Codocedo,J.F., Montecinos-Oliva,C. and Inestrosa,N.C.
  TITLE     Wnt-related SynGAP1 is a neuroprotective factor of glutamatergic
            synapses against Abeta oligomers
  JOURNAL   Front Cell Neurosci 9, 227 (2015)
   PUBMED   26124704
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5868)
  AUTHORS   Song,B., Meng,F., Yan,X., Guo,J. and Zhang,G.
  TITLE     Cerebral ischemia immediately increases serine phosphorylation of
            the synaptic RAS-GTPase activating protein SynGAP by
            calcium/calmodulin-dependent protein kinase II alpha in hippocampus
            of rats
  JOURNAL   Neurosci Lett 349 (3), 183-186 (2003)
   PUBMED   12951199
REFERENCE   6  (bases 1 to 5868)
  AUTHORS   Kim,J.H., Lee,H.K., Takamiya,K. and Huganir,R.L.
  TITLE     The role of synaptic GTPase-activating protein in neuronal
            development and synaptic plasticity
  JOURNAL   J Neurosci 23 (4), 1119-1124 (2003)
   PUBMED   12598599
REFERENCE   7  (bases 1 to 5868)
  AUTHORS   Komiyama,N.H., Watabe,A.M., Carlisle,H.J., Porter,K.,
            Charlesworth,P., Monti,J., Strathdee,D.J., O'Carroll,C.M.,
            Martin,S.J., Morris,R.G., O'Dell,T.J. and Grant,S.G.
  TITLE     SynGAP regulates ERK/MAPK signaling, synaptic plasticity, and
            learning in the complex with postsynaptic density 95 and NMDA
            receptor
  JOURNAL   J Neurosci 22 (22), 9721-9732 (2002)
   PUBMED   12427827
REFERENCE   8  (bases 1 to 5868)
  AUTHORS   Li,W., Okano,A., Tian,Q.B., Nakayama,K., Furihata,T., Nawa,H. and
            Suzuki,T.
  TITLE     Characterization of a novel synGAP isoform, synGAP-beta
  JOURNAL   J Biol Chem 276 (24), 21417-21424 (2001)
   PUBMED   11278737
REFERENCE   9  (bases 1 to 5868)
  AUTHORS   Chen,H.J., Rojas-Soto,M., Oguni,A. and Kennedy,M.B.
  TITLE     A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by
            CaM kinase II
  JOURNAL   Neuron 20 (5), 895-904 (1998)
   PUBMED   9620694
  REMARK    Erratum:[Neuron 2002 Jan 3;33(1):151]
REFERENCE   10 (bases 1 to 5868)
  AUTHORS   Kim,J.H., Liao,D., Lau,L.F. and Huganir,R.L.
  TITLE     SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90
            protein family
  JOURNAL   Neuron 20 (4), 683-691 (1998)
   PUBMED   9581761
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000020.1.
            
            On Nov 8, 2016 this sequence version replaced NM_181092.4.
            
            Summary: This gene encodes a Ras GTPase activating protein that is
            a member of the N-methyl-D-aspartate receptor complex. The
            N-terminal domain of the protein contains a Ras-GAP domain, a
            pleckstrin homology domain, and a C2 domain that may be involved in
            binding of calcium and phospholipids. The C-terminal domain
            consists of a ten histidine repeat region, serine and tyrosine
            phosphorylation sites, and a T/SXV motif required for postsynaptic
            scaffold protein interaction. The encoded protein negatively
            regulates Ras, Rap and
            alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor
            trafficking to the postsynaptic membrane to regulate synaptic
            plasticity and neuronal homeostasis. Homozygous null mutations in
            mice result in early post-embryonic lethality, while heterozygous
            mutant mice display a variety of phenotypes that include learning
            and memory defects, hyperactivity, and audiogenic seizures.
            [provided by RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF048976.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               JAXUCZ010000020.1  5028226-5028360
            136-257             JAXUCZ010000020.1  5031576-5031697
            258-363             JAXUCZ010000020.1  5033506-5033611
            364-455             JAXUCZ010000020.1  5039363-5039454
            456-577             JAXUCZ010000020.1  5039823-5039944
            578-731             JAXUCZ010000020.1  5041416-5041569
            732-830             JAXUCZ010000020.1  5041772-5041870
            831-1454            JAXUCZ010000020.1  5043710-5044333
            1455-1599           JAXUCZ010000020.1  5044443-5044587
            1600-1744           JAXUCZ010000020.1  5044784-5044928
            1745-1981           JAXUCZ010000020.1  5046327-5046563
            1982-2183           JAXUCZ010000020.1  5046649-5046850
            2184-2362           JAXUCZ010000020.1  5047059-5047237
            2363-2404           JAXUCZ010000020.1  5047874-5047915
            2405-3476           JAXUCZ010000020.1  5048243-5049314
            3477-3650           JAXUCZ010000020.1  5049789-5049962
            3651-3862           JAXUCZ010000020.1  5051210-5051421
            3863-3953           JAXUCZ010000020.1  5052938-5053028
            3954-5868           JAXUCZ010000020.1  5056605-5058519
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..5868
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /note="synaptic Ras GTPase activating protein 1"
                     /db_xref="GeneID:192117"
                     /db_xref="RGD:621090"
     exon            1..135
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..3995
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /note="isoform 1 is encoded by transcript variant 1; ras
                     GTPase-activating protein SynGAP; p135 SynGAP; neuronal
                     RasGAP; synaptic Ras-GAP 1; ras/Rap GTPase-activating
                     protein SynGAP"
                     /codon_start=1
                     /product="ras/Rap GTPase-activating protein SynGAP isoform
                     1"
                     /protein_id="NP_851606.3"
                     /db_xref="GeneID:192117"
                     /db_xref="RGD:621090"
                     /translation="MSRSRASIHRGSIPAMSYAPFRDVRGPPMHRTQYVHSPYDRPGW
                     NPRFCIISGNQLLMLDEDEIHPLLIRDRRSESSRNKLLRRTVSVPVEGRPHGEHEYHL
                     GRSRRKSVPGGKQYSMEAAPAAPFRPSQGFLSRRLKSSIKRTKSQPKLDRTSSFRQIL
                     PRFRSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVHSSILGQEF
                     CFEVTTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPP
                     KKRYYCELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKK
                     RKKDKAGYVGLVTVPVATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGK
                     GKGGCPAVRLKARYQTMSILPMELYKEFAEYVTNHYRMLCAVLEPALNVKGKEEVASA
                     LVHILQSTGKAKDFLSDMAMSEVDRFMEREHLIFRENTLATKAIEEYMRLIGQKYLKD
                     AIGEFIRALYESEENCEVDPIKCTASSLAEHQANLRMCCELALCKVVNSHCVFPGELK
                     EVFASWRLRCAERGREDIADRLISASLFLRFLCPAIMSPSLFGLMQEYPDEQTSRTLT
                     LIAKVIQNLANFSKFTSKEDFLGFMNEFLELEWGSMQQFLYEISNLDTLTNSSSFEGY
                     IDLGRELSTLHALLWEVLPQLSKEALLKLGPLPRLLSDISTALRNPNIQRQPSRQSER
                     ARSQPMVLRGPSAEMQGYMMRDLNSSIDLQSFMARGLNSSMDMARLPSPTKEKPPPPP
                     PGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKSVSMLDLQGDGPGGRLN
                     SSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLSQMGVTTD
                     GVPAQQLRIPLSFQNPLFHMAADGPGPPAGHGGSSGHGPPSSHHHHHHHHHHRGGEPP
                     GDTFAPFHGYSKSEDLSTGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQDNLQH
                     MLSPPQITIGPQRPAPSGPGGGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKPRPSS
                     GNLLQSPEPSYGPARPRQQSLSKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPSTLNP
                     TMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLDRVKEYEEEIHS
                     LKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQVEKDSQIKSI
                     IGRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV"
     exon            136..257
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            258..363
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            364..455
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            456..577
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            578..731
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            732..830
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1454
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1599
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1744
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1981
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2183
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2362
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2404
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..3476
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3650
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3862
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3863..3953
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..5868
                     /gene="Syngap1"
                     /gene_synonym="Syngap"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctttctctcc ccctctttct ctcttctttt tcttccccgt cccccccgcc ccctccccct
       61 caggcctgat gagcaggtct cgagcctcca tccatcgggg gagcatcccc gcgatgtcct
      121 atgccccctt cagagatgta cggggacccc ctatgcaccg aacccaatac gttcattccc
      181 cgtatgaccg tcccggttgg aacccccggt tctgcatcat ctctgggaac cagctgctca
      241 tgctggatga ggatgagata cacccccttc tgatccgcga ccggaggagc gagtccagcc
      301 gaaacaaact gctgagacgc accgtctctg tgccagtgga ggggcggccc cacggcgagc
      361 atgaatacca cttgggtcgc tcgaggagga agagtgtccc cggggggaaa cagtacagca
      421 tggaagccgc ccccgctgcg cccttccggc cctcgcaagg cttcctgagc cggaggctaa
      481 aaagctccat caaacgtaca aagtcacaac ccaaacttga ccggaccagc agctttcgac
      541 agatcctgcc tcgcttccga agtgctgacc atgaccgggc ccggctgatg cagagcttca
      601 aggagtctca ctcccatgag tccctgctga gtcccagcag tgctgctgag gccctggagc
      661 tcaacctgga tgaagactcc attatcaagc cagtacacag ctccatcctg ggccaggagt
      721 tctgctttga ggtaacaaca tcgtctggga caaaatgttt tgcctgtcgg tctgcagccg
      781 aaagggacaa atggattgag aatctacaga gggctgtgaa acccaacaag gacaacagcc
      841 gccgggtaga taacgtgctg aaactatgga tcatagaagc tcgagagctg ccccccaaga
      901 agcgatatta ctgcgagtta tgcctggacg acatgctcta tgcacggacc acttccaagc
      961 cccgctcagc ctcaggagac actgtctttt ggggcgagca cttcgagttt aacaacctgc
     1021 ctgctgtccg ggcgctgcgg ctgcatctgt accgtgactc ggacaaaaag cggaagaagg
     1081 acaaggcagg ctacgttggc ctggtgactg ttccagtggc caccctggct gggcgccact
     1141 tcacagagca gtggtacccc gtgaccctgc caacaggaag tgggggctct gggggtatgg
     1201 gctcgggggg aggagggggg tcagggggcg gctcaggggg caaagggaaa ggaggctgtc
     1261 ctgctgtgcg gctgaaggcc cgttaccaga caatgagtat cctgcccatg gagctatata
     1321 aggagtttgc agaatatgtg accaaccact accgcatgct gtgtgccgtg ctggagcccg
     1381 ccctcaatgt caagggcaag gaggaggtcg ctagtgcact ggttcacatc ctgcaaagca
     1441 caggcaaggc caaggacttc ctttcagaca tggccatgtc agaggtagac cggttcatgg
     1501 agcgggaaca cctcatattc cgcgagaaca cgctcgccac taaagccata gaagagtata
     1561 tgagactgat tggccagaaa tacctcaagg atgccattgg ggagttcatc cgggctctgt
     1621 atgaatctga ggagaactgt gaagtagacc ccatcaagtg cacagcgtcc agtctggcag
     1681 agcaccaggc caacctgcgg atgtgctgtg agttggccct gtgcaaggtg gtcaactccc
     1741 attgcgtgtt cccgggggag ctgaaggagg tgtttgcatc atggcggctg cgctgtgcag
     1801 agcggggccg ggaggacatt gctgacaggc tgatcagcgc ctcgctcttc ctgcgcttcc
     1861 tctgcccggc catcatgtcg cccagtctgt ttggactgat gcaggagtac ccagatgagc
     1921 agacctcacg aaccctcacc ctcatcgcca aggttatcca gaacctggcc aacttttcca
     1981 agtttacctc aaaggaggac ttcctgggct tcatgaacga gtttctggag ctggagtggg
     2041 gttctatgca gcaattcttg tatgagatat ccaacctgga cacactgacc aacagcagca
     2101 gttttgaggg ctacatagac ttgggccgcg agctctccac acttcacgcc ctgctctggg
     2161 aggtgctgcc ccagctcagc aaggaagccc tcctgaagct gggcccgctg ccccggctcc
     2221 tcagcgacat cagcacagcc ctgaggaacc ctaacatcca aaggcagccg agccgccaga
     2281 gcgagcgcgc tcggtctcag cccatggtgc tgcgcgggcc gtcagccgag atgcagggct
     2341 acatgatgcg ggacctcaac agctccatcg accttcagtc cttcatggct cgaggcctca
     2401 acagctctat ggacatggct cgcctcccct ccccaaccaa ggagaaaccc ccgccgcccc
     2461 ctcccggtgg gggtaaagac ctgttctatg tgagccggcc accactggcc cggtcctccc
     2521 cagcatactg cacgagcagc tcggacatca cagagccgga gcagaagatg ctgagtgtca
     2581 acaagagtgt gtccatgctg gacctgcagg gcgacgggcc tgggggccgc cttaacagca
     2641 gtagtgtttc caacctggca gctgttgggg acctgttgca ctcaagccag gcttcactga
     2701 cagcagcctt ggggttgcgg cctgcacctg ccgggcgcct ctcccaaggg agtggctctt
     2761 ccatcacagc agccggcatg cgcctcagcc agatgggtgt cactacggat ggtgtccccg
     2821 cccagcaact gcgcatccct ctttccttcc agaaccctct cttccatatg gctgccgatg
     2881 gaccagggcc cccagcaggc catggaggga gcagtggcca tggtccacct tcctcccatc
     2941 accaccacca ccaccatcac catcaccgag ggggagaacc cccaggggac acttttgccc
     3001 cgttccatgg ctatagcaag agcgaggacc tctctacagg ggtccctaag ccccctgcgg
     3061 cctccatcct tcacagccac agctacagtg atgagtttgg accctctggt actgatttta
     3121 cccgtcggca gctctcactt caggacaacc tacagcacat gctctccccg ccccagatca
     3181 ccatcggtcc ccagaggcca gctccctcag ggccaggagg gggcagtggt gggggcagtg
     3241 gtgggggcgg tgggggccag ccacctccct tgcagagggg caaatctcag cagttgacag
     3301 tgagtgctgc ccagaaaccc cggccgtcca gcgggaacct attgcagtcc ccggaaccaa
     3361 gttatggtcc tgcccgtcca cggcaacaga gcctcagcaa agagggcagc attgggggca
     3421 gcgggggcag cggtggcgga gggggtgggg ggctcaagcc ctccatcacc aagcagcatt
     3481 cccagactcc atccacgctg aaccccacga tgccggcctc ggagcggact gtagcctggg
     3541 tgtccaatat gcctcacctg tccgctgaca tcgagagtgc acacattgag cgggaagagt
     3601 acaagctgaa ggagtactcg aagtccatgg acgagagccg actggacagg gtgaaggagt
     3661 acgaggagga gatccactca ctgaaggaaa ggctacacat gtccaaccgg aagctggaag
     3721 agtacgagcg gaggctgctg tcccaggaag agcagaccag caagatcctg atgcagtacc
     3781 aagcccgcct ggagcagagc gagaagcgct tgaggcagca gcaggtggag aaggactccc
     3841 agatcaagag catcattggc aggctgatgc tggtggagga ggagctgcgc cgggaccacc
     3901 ccgccatggc tgagccgctg cctgaaccca agaagaggct gctcgacgct cagagaggca
     3961 gcttcccccc ttgggtccaa caaacccgcg tgtgacgctg gccccacctt ggaacggcct
     4021 ggccccccca gccccacccc ccccaccccg gctgcagatc acagagaacg gcgagttccg
     4081 gaacaccgca gaccactagc ccacccagca tcacagacct ccttccctgt gcaccctacc
     4141 ccggcccacc cagcgtcaca gacctccttc ccagtgcacc cgaccctgga acatcaccaa
     4201 ccaccaggac tggacgtcac caagggacag cgggattgtc tcccttaacg cctccttggg
     4261 gcacccatct gtcaacccca ctgctccatt ccaggaggga gagtgggacc ctcagctgcc
     4321 ctctcacccc aggacaccac ctaccccaca cagacccctt cactctgggg tgctatcccc
     4381 atcctctgcc tcacagttcc cctgagcact gggggacaga ccctcacccc cacactgggg
     4441 gtgtggcacc tccaaacttt caacttcagg gtgatttttt tagcagtaac cagagctgac
     4501 aatctataac ccccctctac caccccattt tggcctcccc tgcccccctt gttatgggga
     4561 ggggaccccg ggtgaggggg ccctattacc ccttgatttc tcaggagcgt ctgggggggc
     4621 tcagcacgca caactccttc tcctctttcc actcttacat taactctccc cacccagaac
     4681 ccagatgggg tggagggggc caccggggca gggagggggc ggcaaggggg gaatgggagt
     4741 tgcctcccct tcttcccaca cctgatctgc tcttggctgg tcccagagcg gggtgagggg
     4801 gcttatgccc ccccctcccc cagtgtgttg ggtggggtgg aattgaggtt cggatgaggg
     4861 ggttagggtt taggaggcca tgtgtgttgg gtcgtagagg acagattagc tgatctgccc
     4921 tactccgaca cacagccagt cctctacccc ttccctctct tgtctctact cccatgggga
     4981 ggggggaact tactctagga aaagccatgt ctctctcccc caggatgggg ggacctgtgt
     5041 tggaggatgg gtgtttgggg gcccccttac atgactctgt cccctggggg aggtaggaca
     5101 gggctgggct tccctctcac cctccccctc acccagtctc cctccctccc gccagctcca
     5161 cgctttttcg gtgttttctg tacatagctc tctggcggaa caggggaggt aggatggatg
     5221 gggtctgggt gggtaaacca tgaaggggag aaacccctcc tgggtacccc tcttctctga
     5281 ctgctgccct ctacccagcc ttgtctcttc accccttctt gcgtttggta ttgagaattc
     5341 ttcccagact ccagactcga cctttctttt gtatgaacag ctctccttca aacttacccc
     5401 tctacataca cccacccagc cggggccaaa tttatactta tataaaagtt gtaaatatgt
     5461 gaaattttat cccattccct ctcctctgct tccctacatc aggccttacc cttggacctc
     5521 ctccctcctc tttggctgtt gtaattatct ggggtttgta ctgtacataa ttggggtggg
     5581 gggctgggag gcgacccctc tgtacagagc ttcctggctc cctagcccca cccctcattt
     5641 ccttccccac ccaccacctt aagagtaggg agaagatctt cttactcatt ttttttgctg
     5701 tttgtttact cgttctcctt tctgctcctg ctcccagact cttggtcccc acccccatcc
     5761 ccccacaccc cctcttttct cccactcccg ggtccatttc cctttttttt tctggagtgt
     5821 gtggtgaaac agaaaaatca tgtttaataa acggagattg ttctttta
//
